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1. 发明申请

US20080068589A1 OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS 有权
标题翻译：光学显微镜与光电性能光标签
公开(公告)号：US20080068589A1
公开(公告)日：2008-03-20
申请号：US11944289
申请日：2007-11-21
申请人： Harald Hess , Robert Betzig
发明人： Harald Hess , Robert Betzig
IPC分类号： G01N1/00
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6458 , G01N21/648 , G01N33/582 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58
摘要： Spatially-structured activation radiation having relatively high- and low-intensity regions is provided to a sample that includes phototransformable optical labels (“PTOLs”). A subset of the PTOLs located predominately at relatively high intensity regions of the spatially-structured activation radiation is activated. Spatially-structured excitation radiation is provided to the activated PTOLs, and the excitation radiation is structured so that one or more relatively high intensity regions of the excitation radiation at least partially overlap one or more relatively high intensity regions of the activating radiation to excite PTOLs located at the relatively high intensity excitation radiation regions. Radiation emitted from the activated and excited PTOLs is detected with imaging optics. Intensities and spatial structures of activating radiation and excitation radiation are controlled so radiation emitted from activated and excited PTOLs is emitted substantially from a volume that is comparable to or less than a diffraction-limited resolution volume (“DLRV”) of the imaging optics.
摘要翻译：将具有相对高和低强度区域的空间结构的激活辐射提供给包括光变形光学标签（“PTOL”）的样品。主要位于空间结构的激活辐射的相对高强度区域的PTOL的子集被激活。空间结构的激发辐射被提供给激活的PTOL，并且激发辐射被构造成使得激发辐射的一个或多个相对较高强度的区域至少部分地与激活辐射的一个或多个相对高强度的区域重叠以激发所定位的PTOL 在相对高强度的激发辐射区域。使用成像光学元件检测从激活和激发的PTOL发射的辐射。激活辐射和激发辐射的强度和空间结构被控制，使得从激活和激发的PTOL发射的辐射基本上从与成像光学器件的衍射限制分辨率体积（“DLRV”）相当或小于的体积发射。

2. 发明申请

US20080068588A1 OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS 有权
标题翻译：光学显微镜与光电性能光标签
公开(公告)号：US20080068588A1
公开(公告)日：2008-03-20
申请号：US11944285
申请日：2007-11-21
申请人： Harald Hess , Robert Betzig
发明人： Harald Hess , Robert Betzig
IPC分类号： G01N1/00
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6458 , G01N21/648 , G01N33/582 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58
摘要： A method of imaging with an optical system characterized by a diffraction-limited resolution volume is disclosed. In a sample that includes a plurality of phototransformable optical labels (“PTOLs”) distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system, a first subset of the PTOLs in the sample are activated, and the density of the activated PTOLs in the first subset is less than the inverse of the diffraction-limited resolution volume. A first portion of the PTOLs in the first subset of PTOLs is excited. Radiation emitted from the activated and excited PTOLs in the first portion of PTOLs is detected with the imaging optics, and locations of activated and excited PTOLs in the first portion of PTOLs is determined with a sub-diffraction-limited accuracy based on the detected radiation emitted from the activated and excited PTOLs.
摘要翻译：公开了一种以衍射极限分辨率体积为特征的光学系统成像的方法。在包含分布在样品中的密度大于光学系统的衍射限制分辨率体积的倒数的多个光变形光学标签（“PTOL”）的样品中，样品中的PTOL的第一子集被激活，并且第一子集中激活的PTOL的密度小于衍射限制分辨率体积的倒数。 PTOL的第一子集中的PTOL的第一部分被激发。使用成像光学元件检测PTOL第一部分中激活和激发的PTOL发射的辐射，并且PTOL的第一部分中激活和激发的PTOL的位置基于检测到的辐射发射的亚衍射极限精度来确定从激活和兴奋的PTOL。

3. 发明申请

US20080070323A1 OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS 有权
标题翻译：光学显微镜与光电性能光标签
公开(公告)号：US20080070323A1
公开(公告)日：2008-03-20
申请号：US11944321
申请日：2007-11-21
申请人： Harald Hess , Robert Betzig
发明人： Harald Hess , Robert Betzig
IPC分类号： G01N33/558
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6458 , G01N21/648 , G01N33/582 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58
摘要： An apparatus includes a position-sensitive detector to detect intensities of radiation as a function of position on the detector, and an optical system, characterized by a diffraction-limited resolution volume, adapted for imaging light emitted from activated and excited phototransformable optical labels (“PTOLs”) in a sample onto the position sensitive-detector. A first light source provides activation radiation to the sample to activate a subset of the PTOLs that are distributed in the sample with a density greater than an inverse of the diffraction-limited resolution volume of the optical system. A second light source provides excitation radiation to the sample to excite a portion of the PTOLs in the subset of the PTOLs. A controller controls one both of the activation radiation and the excitation radiation provided to the sample such that a density of PTOLs in the portion of the PTOLs is less than the inverse of the diffraction-limited resolution volume.
摘要翻译：一种装置包括：位置敏感检测器，用于检测作为检测器上的位置的函数的辐射强度，以及光学系统，其特征在于衍射限制分辨率体积，其适于对从激活和激发的光变换光学标签（“ PTOL“）放置在位置敏感检测器上。第一光源向样品提供激活辐射以激活分布在样品中的密度大于光学系统的衍射限制分辨率体积的倒数的PTOL子集。第二光源向样品提供激发辐射以激发PTOL的子集中的PTOL的一部分。控制器控制提供给样品的激活辐射和激发辐射之一，使得PTOL部分中的PTOL的密度小于衍射限制分辨率体积的倒数。

4. 发明申请

US20080070322A1 OPTICAL MICROSCOPY WITH PHOTOTRANSFORMABLE OPTICAL LABELS 有权
标题翻译：光学显微镜与光电性能光标签
公开(公告)号：US20080070322A1
公开(公告)日：2008-03-20
申请号：US11944280
申请日：2007-11-21
申请人： Harald Hess , Robert Betzig
发明人： Harald Hess , Robert Betzig
IPC分类号： G01N33/558
CPC分类号： G01N21/6428 , G01N21/64 , G01N21/6458 , G01N21/648 , G01N33/582 , G01N2021/6419 , G01N2021/6421 , G01N2021/6439 , G01N2021/6441 , G02B21/16 , G02B21/367 , G02B27/58
摘要： First activation radiation is provided to a sample that includes fluorescent proteins (“FPs”) to activate a first subset of the FPs in the sample. First excitation radiation is provided to the first subset of FPs in the sample to excite at least some of the activated FPs, and radiation emitted from activated and excited FPs within the first subset of FPs is detecting with imaging optics. The first activation radiation is controlled such that the mean volume per activated FPs in the first subset is greater than or approximately equal to a diffraction-limited resolution volume (“DLRV”) of the imaging optics.
摘要翻译：第一激活辐射被提供给包括荧光蛋白（“FP”）的样品，以激活样品中FP的第一子集。第一激发辐射被提供给样本中的FP的第一子集，以激励至少一些激活的FP，并且从FP的第一子集内的激活和激发的FP发射的辐射是用成像光学器件进行检测的。控制第一激活辐射，使得第一子集中每个激活的FP的平均体积大于或近似等于成像光学器件的衍射限制分辨率体积（“DLRV”）。

5. 发明申请

US20080271302A1 Machining center 审中-公开
标题翻译：加工中心
公开(公告)号：US20080271302A1
公开(公告)日：2008-11-06
申请号：US11799818
申请日：2007-05-03
申请人： Robert Betzig , Brian Singleton
发明人： Robert Betzig , Brian Singleton
IPC分类号： B23P21/00
CPC分类号： B23Q39/024 , B23Q2039/006 , Y10T29/5124 , Y10T408/3806 , Y10T408/3833 , Y10T408/385 , Y10T409/305264 , Y10T409/30532
摘要： A machining center is provided enabling a high degree of machining automation. The machining center includes a master machining head including multiple spindles mounted fixedly to the machining base. A workpiece transport system transports workpieces between machining stations and also moves it toward and away from the machining stations which are preferable fixedly mounted. Registration pins mounted to the master machine head register with a receiving socket carried by the workpiece platform or associated workpiece fixture. A robot actuator is provided which loads and unloads workpieces with the machining center 10 and can also potentially provide other functions such as tool inspection tool changing, workpiece repositioning.
摘要翻译：提供加工中心，可实现高度的加工自动化。加工中心包括主加工头，包括固定在加工基座上的多个主轴。工件运输系统在加工站之间输送工件，并且将其移动到远离加工站，优选地固定地安装。安装在主机头上的定位销用工件平台或相关工件夹具承载的接收插座。提供了一种机器人致动器，其利用加工中心10加载和卸载工件，并且还可以潜在地提供其他功能，例如刀具检查工具更换，工件重新定位。

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