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1. 发明授权

US06271342B1 Sugar-modified cytostatics 失效
标题翻译：糖改性细胞抑制剂
公开(公告)号：US06271342B1
公开(公告)日：2001-08-07
申请号：US08930546
申请日：1997-09-25
申请人： Hans-Georg Lerchen , Karsten von dem Bruch , Uwe Petersen , Jörg Baumgarten , Norbert Piel , Horst-Peter Antonicek , Walter Weichel , Michael Sperzel , Klaus Dieter Bremm
发明人： Hans-Georg Lerchen , Karsten von dem Bruch , Uwe Petersen , Jörg Baumgarten , Norbert Piel , Horst-Peter Antonicek , Walter Weichel , Michael Sperzel , Klaus Dieter Bremm
IPC分类号： A61K3814
CPC分类号： C07D401/04 , A61K31/02 , C07D487/04 , C07H15/203 , C07K9/001 , Y02P20/55
摘要： The invention relates to cytostatics which, by modification with sugar, are tumor-specific. Suitable spacers ensure serum stability and at the same time an intracellular action.
摘要翻译：本发明涉及通过用糖改性的肿瘤特异性的细胞抑制剂。合适的间隔物确保血清稳定性，并同时进行细胞内作用。

2. 发明申请

US20060263838A1 Glucose dehydrogenase and production thereof 失效
标题翻译：葡萄糖脱氢酶及其生产
公开(公告)号：US20060263838A1
公开(公告)日：2006-11-23
申请号：US10555903
申请日：2004-04-27
申请人： Ruth Meissner , Walter Weichel , Rainhard Koch , Irina Bachmatova , Liucija Marcinkeviciene , Rita Meskiene , Rasa Semenaite , Vida Casaite , Rolandas Meskys
发明人： Ruth Meissner , Walter Weichel , Rainhard Koch , Irina Bachmatova , Liucija Marcinkeviciene , Rita Meskiene , Rasa Semenaite , Vida Casaite , Rolandas Meskys
IPC分类号： C12Q1/54 , C12P21/06 , C12N9/02 , C12N15/74 , C12N1/21
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , Y10T436/144444
摘要： The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from Acinetobacter and to a process for their preparation by overexpression in suitable microbial expression systems.
摘要翻译：本发明涉及来自不动杆菌的新型PQQ依赖性可溶性葡萄糖脱氢酶（sPQQGDH）及其在合适的微生物表达系统中通过过表达的制备方法。

3. 发明授权

US08580547B2 Variants of PQQ-dependent glucose dehydrogenase having improved substrate specificity 失效
标题翻译：具有改善的底物特异性的PQQ依赖性葡萄糖脱氢酶的变体
公开(公告)号：US08580547B2
公开(公告)日：2013-11-12
申请号：US12999465
申请日：2009-06-17
申请人： Walter Weichel , Volker Möhrle , Markus Schindler , Rainhard Koch , Rolandas Meskys
发明人： Walter Weichel , Volker Möhrle , Markus Schindler , Rainhard Koch , Rolandas Meskys
IPC分类号： C12Q1/32 , C12N9/00 , C12N9/04 , C12N1/20 , C07H21/04
CPC分类号： C12Q1/006 , C12N9/0006 , C12Q1/32 , C12Y101/01069 , C12Y101/05002
摘要： The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) which have an increased substrate specificity compared with the wild type, and also to methods for production and identification thereof.
摘要翻译：本发明涉及与野生型相比具有增加的底物特异性的新型PQQ依赖性可溶性葡萄糖脱氢酶（sPQQGDH），以及其生产和鉴定方法。

4. 发明授权

US5837200A Sorting device for biological cells or viruses 失效
标题翻译：用于生物细胞或病毒的分选装置
公开(公告)号：US5837200A
公开(公告)日：1998-11-17
申请号：US653143
申请日：1996-05-24
申请人： Edgar Diessel , Walter Weichel
发明人： Edgar Diessel , Walter Weichel
IPC分类号： C12M1/00 , B01L3/00 , B03C1/035 , C12M1/42 , C12Q1/04 , C12Q1/24 , C12Q1/70 , F15C1/08 , G01N15/14 , G01N33/569 , G01N33/00
CPC分类号： F15C1/08 , B01L3/502761 , B03C1/035 , B01L2200/0647 , B01L2300/0864 , B01L2400/043 , B01L2400/0439 , G01N2015/149 , Y10S209/906 , Y10S435/808
摘要： The sorting device is able to sort biological objects, particularly cells or viruses, in liquids as a function of physically measurable criteria or properties. The sorting apparatus is provided with a feed inlet (1) for the biological liquid which branches into a microstructured system of multi-parallel main channels (3) and sorting modules (4) each with a switch unit for distribution to two different outlet channels (19,20). Inside a sorting module (4), at least one sensor (9,10) is arranged on each main channel, and a sorting actuator (12) controlled by the relevant sensor is arranged on each switch unit (11). Finally, each outlet channel (20) is connected to a collecting channel (6) for the unselected biological cells or viruses, and each outlet channel (19) is connected to a collecting channel (5) for the selected cells or viruses. If the cells or viruses are magnetically marked, it is possible to dispense with the sensor (9,10) on the main channels (3). In that case, the sorting actuators arranged on the switch units are designed as magnetic deflection modules (37,38). In this case, the magnetic deflection modules undertake direct classification or selection as a function of the magnetic properties of the biological objects to be sorted.
摘要翻译：分选装置能够根据物理上可测量的标准或性质将生物体，特别是细胞或病毒分离在液体中。分拣装置设置有用于生物液体的进料口（1），其分支成多平行主通道（3）和分拣模块（4）的微结构化系统，每个分配模块具有开关单元，用于分配到两个不同的出口通道 19,20）。在分拣模块（4）内，在每个主通道上布置有至少一个传感器（9,10），并且在每个开关单元（11）上布置由相关传感器控制的分拣机构（12）。最后，每个出口通道（20）连接到用于未选择的生物细胞或病毒的收集通道（6），并且每个出口通道（19）连接到用于所选细胞或病毒的收集通道（5）。如果细胞或病毒被磁性标记，则可以省去主通道（3）上的传感器（9,10）。在这种情况下，布置在开关单元上的排序致动器被设计为磁偏转模块（37,38）。在这种情况下，磁偏转模块根据要分选的生物体的磁特性进行直接分类或选择。

5. 发明申请

US20110236920A1 NOVEL VARIANTS OF PQQ-DEPENDENT GLUCOSE DEHYDROGENASE HAVING IMPROVED SUBSTRATE SPECIFICITY 失效
标题翻译：具有提高基质特性的PQQ依赖性葡萄糖脱氢酶的新变化
公开(公告)号：US20110236920A1
公开(公告)日：2011-09-29
申请号：US12999465
申请日：2009-06-17
申请人： Walter Weichel , Volker Möhrle , Markus Schindler , Rainhard Koch , Rolandas Meskys
发明人： Walter Weichel , Volker Möhrle , Markus Schindler , Rainhard Koch , Rolandas Meskys
IPC分类号： C12Q1/32 , C12N9/04 , C07H21/00 , C12N15/63
CPC分类号： C12Q1/006 , C12N9/0006 , C12Q1/32 , C12Y101/01069 , C12Y101/05002
摘要： The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) which have an increased substrate specificity compared with the wild type, and also to methods for production and identification thereof.
摘要翻译：本发明涉及与野生型相比具有增加的底物特异性的新型PQQ依赖性可溶性葡萄糖脱氢酶（sPQQGDH），以及其生产和鉴定方法。

6. 发明授权

US07432096B2 Glucose dehydrogenase and production thereof 失效
标题翻译：葡萄糖脱氢酶及其生产
公开(公告)号：US07432096B2
公开(公告)日：2008-10-07
申请号：US10555903
申请日：2004-04-27
申请人： Ruth Meissner , Walter Weichel , Rainhard Koch , Irina Bachmatova , Liucija Marcinkeviciene , Rita Meskiene , Rasa Semenaite , Vida Casaite , Rolandas Meskys
发明人： Ruth Meissner , Walter Weichel , Rainhard Koch , Irina Bachmatova , Liucija Marcinkeviciene , Rita Meskiene , Rasa Semenaite , Vida Casaite , Rolandas Meskys
IPC分类号： C12N9/02 , C12N1/12 , C12Q1/26 , C12P21/06 , G01N33/00 , C07H21/02 , C07H21/04
CPC分类号： C12N9/0006 , C12Q1/006 , C12Q1/32 , Y10T436/144444
摘要： The invention relates to novel PQQ-dependent soluble glucose dehydrogenases (sPQQGDH) from Acinetobacter and to a process for their preparation by overexpression in suitable microbial expression systems.
摘要翻译：本发明涉及来自不动杆菌的新型PQQ依赖性可溶性葡萄糖脱氢酶（sPQQGDH）及其在合适的微生物表达系统中通过过表达的制备方法。

7. 发明授权

US06713264B2 Process and device for the screening of molecules with regard to their individual binding behaviour towards at least one given ligand 失效
标题翻译：关于它们对至少一个给定配体的单独结合行为的分子筛选的方法和装置
公开(公告)号：US06713264B2
公开(公告)日：2004-03-30
申请号：US08891778
申请日：1997-07-14
申请人： Klaus Luttermann , Edgar Diessel , Winfried Kosch , Walter Weichel
发明人： Klaus Luttermann , Edgar Diessel , Winfried Kosch , Walter Weichel
IPC分类号： G01N3353
CPC分类号： C12M47/04 , B01J2219/00659 , B01J2219/00707 , B01J2219/0072 , G01N15/1468 , Y10S435/808
摘要： The process is used for the screening of molecules from molecule libraries with regard to their individual binding behavior towards at least one given ligand. For this purpose the ligands labelled with a fluorescent dye are mixed with the molecule library which is in the form of a suspension. The mixture is plated out on a two-dimensional substrate (2) after the excess, unbound ligands have been washed out. Then the local fluorescence intensities on the substrate are electro-optically identified in a fluorescence microscope (5) and electronically discriminated in accordance with given selection criteria. The objects selected and localised in this way are then sequentially positioned exactly by a displacement, the coordinates of which are controlled by the image calculator, between the substrate (2) and a separation actuator (20, 21) and are spatially separated from the substrate (2) by the separation actuator (20, 21).
摘要翻译：该方法用于从分子文库筛选针对至少一种给定配体的单个结合行为的分子。为此，用荧光染料标记的配体与悬浮液形式的分子文库混合。在过量的未结合配体被洗掉之后，将混合物镀在二维底物（2）上。然后在荧光显微镜（5）中对基底上的局部荧光强度进行电光学鉴定，并根据给定的选择标准进行电子鉴别。以这种方式选择和定位的物体然后通过基板（2）和分离致动器（20,21）之间的位移（其坐标由图像计算器控制）精确地顺序地定位并且在空间上与基板分离（2）通过分离致动器（20,21）。

8. 发明授权

US5693539A Methods for coating metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same 失效
标题翻译：用于生物材料的高梯度磁选分离的金属基质的涂覆方法及其涂布方法
公开(公告)号：US5693539A
公开(公告)日：1997-12-02
申请号：US251973
申请日：1994-05-31
申请人： Stefan Miltenyi , Andreas Radbruch , Walter Weichel , Werner Muller , Christoph Gottlinger , Klaus Ludwig Meyer
发明人： Stefan Miltenyi , Andreas Radbruch , Walter Weichel , Werner Muller , Christoph Gottlinger , Klaus Ludwig Meyer
IPC分类号： B01J20/32 , B03C1/034 , G01N30/02 , G01N30/52 , G01N30/56 , G01N30/88 , G01N33/543 , G01N33/553 , C12Q1/68 , G01N21/00 , G01N33/552
CPC分类号： B03C1/034 , B01J20/0229 , B01J20/06 , B01J20/24 , B01J20/28004 , B01J20/28007 , B01J20/28009 , B01J20/286 , B01J20/3028 , B01J20/3071 , B01J20/3078 , B01J20/3085 , B01J20/3092 , B01J20/3204 , B01J20/3274 , B01J20/328 , B01J20/3287 , B82Y30/00 , G01N30/52 , G01N33/54326 , B01J2220/54 , B01J2220/58 , G01N2030/027 , G01N2030/567 , G01N2030/8813 , G01N2400/22 , G01N2446/20 , G01N2446/30 , G01N2446/90 , G01N30/02 , Y10S209/932 , Y10S435/803 , Y10S435/824 , Y10S436/803 , Y10S436/807
摘要： Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.
摘要翻译：公开了用于进行高梯度磁选（HGMS）的现有程序和材料的改进。优选的超顺磁性颗粒，任选地涂覆有多糖或其它通常为有机的材料，可以以均匀磁化的均匀组合物制备。该涂层可以方便地缀合到与其纯化或分离期望的生物材料互补的特异性结合部分。此外，公开了形成优异的磁梯度增强载体的塑料涂层基质，以及用于进行HGMS的改进的方法和装置。

9. 发明授权

US5385707A Metal matrices for use in high gradient magnetic separation of biological materials and method for coating the same 失效
标题翻译：用于生物材料的高梯度磁选分离的金属基质及其涂覆方法
公开(公告)号：US5385707A
公开(公告)日：1995-01-31
申请号：US6709
申请日：1993-01-21
申请人： Stefan Miltenyi , Andreas Radbruch , Walter Weichel , Werner Muller , Christoph Gottlinger , Klaus L. Meyer
发明人： Stefan Miltenyi , Andreas Radbruch , Walter Weichel , Werner Muller , Christoph Gottlinger , Klaus L. Meyer
IPC分类号： B01J20/32 , B03C1/034 , G01N30/02 , G01N30/52 , G01N30/56 , G01N30/88 , G01N33/543 , G01N30/48
CPC分类号： B03C1/034 , B01J20/0229 , B01J20/06 , B01J20/24 , B01J20/28004 , B01J20/28007 , B01J20/28009 , B01J20/286 , B01J20/3028 , B01J20/3071 , B01J20/3078 , B01J20/3085 , B01J20/3092 , B01J20/3204 , B01J20/3274 , B01J20/328 , B01J20/3287 , B82Y30/00 , G01N30/52 , G01N33/54326 , B01J2220/54 , B01J2220/58 , G01N2030/027 , G01N2030/567 , G01N2030/8813 , G01N2400/22 , G01N2446/20 , G01N2446/30 , G01N2446/90 , G01N30/02 , Y10S209/932 , Y10S435/803 , Y10S435/824 , Y10S436/803 , Y10S436/807
摘要： Improvements in the existing procedures and materials for conduct of high gradient magnetic separation (HGMS) are disclosed. Superior superparamagnetic particles, optionally coated with a polysaccharide or other, usually organic, materials can be prepared in uniform compositions with homogeneous magnetizations. The coating can conveniently be conjugated to a specific binding moiety complementary to a biological material whose purification or separation is desired. In addition, plastic coated matrices which form superior magnetic gradient-intensifying supports are disclosed, along with improved methods and apparatus to conduct HGMS.
摘要翻译：公开了用于进行高梯度磁选（HGMS）的现有程序和材料的改进。优选的超顺磁性颗粒，任选地涂覆有多糖或其它通常为有机的材料，可以以均匀磁化的均匀组合物制备。该涂层可以方便地缀合到与其纯化或分离期望的生物材料互补的特异性结合部分。此外，公开了形成优异的磁梯度增强载体的塑料涂层基质，以及用于进行HGMS的改进的方法和装置。

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