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1. 发明授权

US06846679B1 Characterizing polypeptides through cleavage and mass spectrometry 失效
标题翻译：通过切割和质谱鉴定多肽
公开(公告)号：US06846679B1
公开(公告)日：2005-01-25
申请号：US09806564
申请日：1999-10-01
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： G01N27/62 , C07K1/12 , C12Q1/02 , G01N33/68 , G01N33/50
CPC分类号： G01N33/6848 , C07K1/12 , G01N33/6842 , Y10T436/24
摘要： Provided is a method for characterizing a polypeptide or a population of polypeptides, which method comprises: (a) contacting a sample comprising one or more polypeptides with a first cleavage agent to generate polypeptide fragments; (b) isolating one or more polypeptide fragments, each fragment comprising the N-terminus or the C-terminus of the polypeptide from which it was fragmented; (c) identifying the isolated fragments by mass spectrometry; (d) repeating steps (a)-(c) on the sample using a second cleavage agent that cleaves at a difference site from the first cleavage agent; and (e) characterizing the one or more polypeptides in the sample from the fragments identified in steps (c) and (d).
摘要翻译：提供了表征多肽或多肽群体的方法，该方法包括：（a）使包含一种或多种多肽的样品与第一切割剂接触以产生多肽片段; （b）分离一个或多个多肽片段，每个片段包含多肽片段的N末端或C末端; （c）通过质谱鉴定分离的片段; （d）使用在与第一切割剂的差异位点切割的第二切割剂对样品重复步骤（a） - （c）; 和（e）从步骤（c）和（d）中鉴定的片段表征样品中的一种或多种多肽。

2. 发明授权

US06391560B1 Screening for functional antisense agents 失效
标题翻译：筛选功能性反义试剂
公开(公告)号：US06391560B1
公开(公告)日：2002-05-21
申请号：US09623390
申请日：2001-03-26
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6811 , C12Q1/6837 , C12Q2521/301
摘要： Provided is a method for identifying a functional antisense agent, which method comprises contacting an RNA with an oligonucleotide probe to form a duplex-containing RNA, contacting the duplex-containing RNA with an RNAase to cleave the duplex-containing RNA, and measuring the kinetics of cleavage.
摘要翻译：提供了用于鉴定功能性反义剂的方法，该方法包括使RNA与寡核苷酸探针接触以形成含双链体的RNA，使含双链体的RNA与RNA酶接触以切割含双链体的RNA，并测量动力学的切割。

3. 发明授权

US06270976B1 Characterizing nucleic acid by mass spectrometry 失效
标题翻译：通过质谱鉴定核酸
公开(公告)号：US06270976B1
公开(公告)日：2001-08-07
申请号：US09508584
申请日：2000-05-04
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6869 , C12Q2525/125 , C12Q2537/143 , C12Q2565/501 , Y10T436/24 , C12Q2563/167 , C12Q2535/101
摘要： The present invention provides a method for analyzing a population of nucleic acid fragments each labeled with a mass label, which method comprises: i) ionizing the population; ii) sorting the ionized population in a mass spectrometer according to mass into sub-populations each containing at least one labeled fragment; iii) cleaving each sub-population to release the mass label associated with each labeled fragment; iv) determining the mass of each released mass label by mass spectroscopy; and v) assigning each mass label to its associated fragment.
摘要翻译：本发明提供了一种用于分析每个用质量标签标记的核酸片段群的方法，该方法包括：i）电离群体; ii）根据质量将质谱仪中的离子化群体分选成各自含有至少一个标记片段的亚群; iii）切割每个亚群以释放与每个标记片段相关联的质量标签; iv）通过质谱确定每个释放的质量标签的质量; 和v）将每个质量标签分配给其相关片段。

4. 发明申请

US20080145850A1 Mass Labels 有权
标题翻译：批量标签
公开(公告)号：US20080145850A1
公开(公告)日：2008-06-19
申请号：US11894735
申请日：2007-08-20
申请人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
发明人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
IPC分类号： C12Q1/68 , G01N37/00 , C40B30/10 , G01N33/53 , G01N33/487 , G01N30/02
CPC分类号： C07H21/00 , C07K1/1077 , C12Q1/6872 , C40B40/00 , G01N33/6803 , G01N2458/15 , Y10T436/13
摘要： Provided herein are sets of mass labels. Each mass label in a set includes: 1) a mass marker moiety; 2) a mass normalisation moiety; and 3) a cleavable linker connecting the mass marker moiety to the mass normalisation moiety. Each mass marker moiety is characterized as having a mass different from that of all other mass marker moieties in the set as determined by mass spectrometry. Further, each mass normalisation moiety ensures that each mass label in the set has substantially the same mass as determined by mass spectrometry.
摘要翻译：本文提供了一组质量标签。一组中的每个质量标签包括：1）质量标记部分; 2）质量归一化部分; 和3）将质量标记部分连接到质量标准化部分的可切割的连接体。每个质量标记物部分的特征在于质量不同于通过质谱法测定的组中所有其它质量标记物部分的质量。此外，每个质量归一化部分确保组中的每个质量标签具有与通过质谱法测定的质量基本相同的质量。

5. 发明授权

US07294456B2 Mass labels 有权
标题翻译：批量标签
公开(公告)号：US07294456B2
公开(公告)日：2007-11-13
申请号：US10221666
申请日：2001-03-14
申请人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
发明人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
IPC分类号： C12Q1/00
CPC分类号： C07H21/00 , C07K1/1077 , C12Q1/6872 , C40B40/00 , G01N33/6803 , G01N2458/15 , Y10T436/13
摘要： Provided herein are sets of mass labels. Each mass label in a set includes 1) a mass marker moiety; 2) a mass normalisation moiety; and 3) a cleavable linker connecting the mass marker moiety to the mass normalisation moiety. Each mass marker moiety is characterised as having a mass different from that of all other mass marker moieties in the set as determined by mass spectrometry. Further, each mass normalisation moiety ensures that each mass label in the set has substantially the same mass as determined by mass spectrometry.
摘要翻译：本文提供了一组质量标签。一组中的每个质量标签包括1）质量标记部分; 2）质量归一化部分; 和3）将质量标记部分连接到质量标准化部分的可切割的连接体。每个质量标记物部分的特征在于质量不同于通过质谱法测定的组中所有其它质量标记物部分的质量。此外，每个质量归一化部分确保组中的每个质量标签具有与通过质谱法测定的质量基本相同的质量。

6. 发明授权

US06649354B1 Catalytically generated mass labels 失效
标题翻译：催化生成大量标签
公开(公告)号：US06649354B1
公开(公告)日：2003-11-18
申请号：US09508049
申请日：2000-05-03
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： G01N3353
CPC分类号： G01N33/531 , C12Q1/6872 , G01N33/58 , Y10S435/814 , Y10S436/805 , Y10S436/823 , Y10S436/824 , Y10S530/81 , Y10S530/811
摘要： The present invention involves a method for assaying a substance. The method of the present invention comprises contacting the substance with an assay agent comprising a catalytic agent to associate the substance with the catalytic agent, contacting the resulting associated substance with a label precursor capable of reacting catalytically with the catalytic agent to release the label, and detecting a mass label. The present invention also involves a kit for assaying a substance. The kit of the present invention comprises an assay agent comprising a catalytic agent, and a label precursor capable of reacting catalytically with the catalytic agent to release a mass label.
摘要翻译：本发明涉及一种用于测定物质的方法。本发明的方法包括使物质与包含催化剂的测定试剂接触以将物质与催化剂缔合，使所得的相关物质与能够与催化剂催化反应的标记前体接触以释放标记;以及检测质量标签。本发明还涉及用于测定物质的试剂盒。本发明的试剂盒包括含有催化剂的测定试剂和能够与催化剂催化反应以释放大量标签的标记前体。

7. 发明授权

US06613511B1 Characterizing DNA 失效
标题翻译：表征DNA
公开(公告)号：US06613511B1
公开(公告)日：2003-09-02
申请号：US09402542
申请日：1999-12-29
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6827 , C12Q1/6837 , C12Q1/6853
摘要： The present invention is drawn to a method for characterizing cDNA, wherein said method produces a sequence signature having the following structure: Adaptor Sequence-Restriction Site-Known Length-Nw-Second Known Length-Nx-Poly-A tail (Known Length).
摘要翻译：本发明涉及用于表征cDNA的方法，其中所述方法产生具有以下结构的序列特征：适配序列 - 限制性位点 - 已知长度-Nw-第二已知长度-Nx-聚A尾（已知长度）。

8. 发明授权

US07825069B2 Mass labels 有权
标题翻译：批量标签
公开(公告)号：US07825069B2
公开(公告)日：2010-11-02
申请号：US11894735
申请日：2007-08-20
申请人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
发明人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
IPC分类号： C40B40/00
CPC分类号： C07H21/00 , C07K1/1077 , C12Q1/6872 , C40B40/00 , G01N33/6803 , G01N2458/15 , Y10T436/13
摘要： Provided herein are sets of mass labels. Each mass label in a set includes: 1) a mass marker moiety; 2) a mass normalization moiety; and 3) a cleavable linker connecting the mass marker moiety to the mass normalization moiety. Each mass marker moiety is characterized as having a mass different from that of all other mass marker moieties in the set as determined by mass spectrometry. Further, each mass normalization moiety ensures that each mass label in the set has substantially the same mass as determined by mass spectrometry.
摘要翻译：本文提供了一组质量标签。一组中的每个质量标签包括：1）质量标记部分; 2）质量归一化部分; 和3）将质量标记部分连接到质量标准化部分的可切割的连接体。每个质量标记物部分的特征在于质量不同于通过质谱法测定的组中所有其它质量标记物部分的质量。此外，每个质量归一化部分确保组中的每个质量标签具有与通过质谱法测定的质量基本相同的质量。

9. 发明授权

US07816304B2 Mass labels 有权
标题翻译：批量标签
公开(公告)号：US07816304B2
公开(公告)日：2010-10-19
申请号：US11841707
申请日：2007-08-20
申请人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
发明人： Gunter Schmidt , Andrew Hugin Thompson , Robert Alexander Walker Johnstone
IPC分类号： C40B40/00
CPC分类号： C07H21/00 , C07K1/1077 , C12Q1/6872 , C40B40/00 , G01N33/6803 , G01N2458/15 , Y10T436/13
摘要： Provided herein are sets of mass labels. Each mass label in a set includes: 1) a mass marker moiety; 2) a mass normalisation moiety; and 3) a cleavable linker connecting the mass marker moiety to the mass normalisation moiety. Each mass marker moiety is characterized as having a mass different from that of all other mass marker moieties in the set as determined by mass spectrometry. Further, each mass normalization moiety ensures that each mass label in the set has substantially the same mass as determined by mass spectrometry.
摘要翻译：本文提供了一组质量标签。一组中的每个质量标签包括：1）质量标记部分; 2）质量归一化部分; 和3）将质量标记部分连接到质量标准化部分的可切割的连接体。每个质量标记物部分的特征在于质量不同于通过质谱法测定的组中所有其它质量标记物部分的质量。此外，每个质量归一化部分确保组中的每个质量标签具有与通过质谱法测定的质量基本相同的质量。

10. 发明授权

US06416951B1 Screening for functional antisense agents 失效
标题翻译：筛选功能性反义试剂
公开(公告)号：US06416951B1
公开(公告)日：2002-07-09
申请号：US09623385
申请日：2001-03-26
申请人： Gunter Schmidt , Andrew Hugin Thompson
发明人： Gunter Schmidt , Andrew Hugin Thompson
IPC分类号： C12Q168
CPC分类号： C12Q1/6816 , C12Q1/6837 , Y10S977/924 , C12Q2565/601 , C12Q2563/173 , C12Q2525/207 , C12Q2565/515
摘要： Provided is a method for identifying a functional antisense agent, which method comprises hybridizing an RNA with an aligonucleotide probe and measuring in real time the kinetics of hybridization, wherein the kinetics are measured by either hybridizing in the presence of an intercalation dye and recording a change in the spectroscopic properties of the dye as hybridizing proceeds, or incorporating a label in either the RNA or the probe, attaching the non-labelled RNA or non-labelled probe to a solid support, generating an evanescent wave in the proximity of the non-labelled RNA or non-labelled probe and recording the increase in a signal generated by interaction of the evanescent wave with the label, as hybridization proceeds.
摘要翻译：提供了一种用于鉴定功能性反义剂的方法，该方法包括将RNA与寡核苷酸探针杂交并实时测量杂交的动力学，其中通过在插入染料存在下杂交并记录变化来测量动力学在作为杂交进行的染料的光谱性质中，或者在RNA或探针中掺入标记，将非标记的RNA或未标记的探针附着到固体支持物上，在非接触的情况下产生ev逝波，标记的RNA或非标记的探针，并记录随着杂交进行而通过ev逝波的相互作用产生的信号与标记的增加。

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