专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08476019B2 Enhanced gene expression in algae 有权
标题翻译：藻类中增强的基因表达
公开(公告)号：US08476019B2
公开(公告)日：2013-07-02
申请号：US13504741
申请日：2010-11-01
申请人： George Oyler , Julian Rosenberg
发明人： George Oyler , Julian Rosenberg
IPC分类号： C12Q1/68
CPC分类号： C12N15/79 , C07K14/415 , C12N15/8216
摘要： The invention provides a system of enhancing the expression of transgenes in algae. Transgenes are engineered to have a binding site for certain proteins in proximity to their promoter, for example a LexA binding site. The algae is also engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.
摘要翻译：本发明提供增强藻类中转基因表达的系统。转基因被设计为具有接近其启动子的某些蛋白质的结合位点，例如LexA结合位点。藻类也被设计成表达与蛋白质融合的核小体变异蛋白质，其与DNA结合位点具有亲和性，起协调作用。一个例子是LexA-p300融合蛋白，其中p300衍生自衣藻。 LexA结合结构域将p300引导到结合位点，p300通过在转基因附近乙酰化组蛋白来松开核小体结构，从而重建局部染色质结构以允许高水平表达。

2. 发明申请

US20120208201A1 ENHANCED GENE EXPRESSION IN ALGAE 有权
标题翻译：在ALGAE中增强基因表达
公开(公告)号：US20120208201A1
公开(公告)日：2012-08-16
申请号：US13504741
申请日：2010-11-01
申请人： George Oyler , Julian Rosenberg
发明人： George Oyler , Julian Rosenberg
IPC分类号： C12Q1/68 , C12N1/12
CPC分类号： C12N15/79 , C07K14/415 , C12N15/8216
摘要： The invention provides a system of enhancing the expression of transgenes in algae. Transgenes are engineered to have a binding site for certain proteins in proximity to their promoter, for example a LexA binding site. The algae is also engineered to express a nucleosome alteration protein fused to a protein with affinity to the DNA binding site acting in coordination. An example is a LexA-p300 fusion protein, where the p300 is derived from Chlamydomonas. The LexA binding domain guides the p300 to the binding site and the p300 loosens the nucleosome structure by acetylating histones within proximity of the transgene, thus remodeling the local chromatin structure to allow for high-level expression.
摘要翻译：本发明提供增强藻类中转基因表达的系统。转基因被设计为具有接近其启动子的某些蛋白质的结合位点，例如LexA结合位点。藻类也被设计成表达与蛋白质融合的核小体变异蛋白质，其与DNA结合位点具有亲和性，起协调作用。一个例子是LexA-p300融合蛋白，其中p300衍生自衣藻。 LexA结合结构域将p300引导到结合位点，p300通过在转基因附近乙酰化组蛋白来松开核小体结构，从而重建局部染色质结构以允许高水平表达。

3. 发明授权

US07632917B2 Methods for identifying inhibitors of botulinum neurotoxins 失效
标题翻译：鉴定肉毒杆菌神经毒素抑制剂的方法
公开(公告)号：US07632917B2
公开(公告)日：2009-12-15
申请号：US11095055
申请日：2005-03-31
申请人： Randall L. Kincaid , George Oyler , Yen Che Tsai , Paul S. Fishman
发明人： Randall L. Kincaid , George Oyler , Yen Che Tsai , Paul S. Fishman
IPC分类号： C07K14/00 , A61K39/08
CPC分类号： C07K14/705 , C12Q1/37 , G01N33/5014 , G01N2500/04
摘要： A system and method for identifying a botulinum neurotoxin inhibitor employing a botulinum neurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. The botulinum neurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express a botulinum neurotoxin substrate complex. The methods of the present invention are adapted for cell based screening to monitor the catalytic activity of a BoNT in living cells and to identify molecules that inhibit the catalytic activity of a BoNT in living cells. Also provided are novel stable cell lines that express the botulinum toxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.
摘要翻译：一种使用具有肽底物的肉毒杆菌神经毒素底物复合物鉴定肉毒杆菌神经毒素抑制剂的系统和方法，优选SNAP-25，所述肽底物一侧的报道结构域和所述肽底物相反侧的固定结构域。肉毒杆菌神经毒素抑制剂通过其降低切割复合物的相对量的能力来鉴定，通过测量与固体支持物结合的复合物的降低来检测。本发明的方法还利用表达肉毒神经毒素底物复合物的新细胞。本发明的方法适用于基于细胞的筛选以监测活细胞中BoNT的催化活性并鉴定抑制活细胞中BoNT的催化活性的分子。还提供了表达肉毒杆菌毒素底物复合物的新型稳定细胞系和能够在哺乳动物细胞内有效表达BoNT活性轻链的病毒载体。

4. 发明授权

US09005911B2 Methods for identifying inhibitors of botulinum neurotoxins 有权
标题翻译：鉴定肉毒杆菌神经毒素抑制剂的方法
公开(公告)号：US09005911B2
公开(公告)日：2015-04-14
申请号：US13345691
申请日：2012-01-07
申请人： Randall L. Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
发明人： Randall L. Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
IPC分类号： G01N33/53 , A61K39/08 , C07K14/705 , C12Q1/37 , G01N33/50
CPC分类号： C07K14/705 , C12Q1/37 , G01N33/5014 , G01N2500/04
摘要： A system and method for identifying a botulinum neurotoxin inhibitor employing a botulinum neurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. The botulinum neurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express a botulinum neurotoxin substrate complex. Also provided are novel stable cell lines that express the botulinum toxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.
摘要翻译：一种使用具有肽底物的肉毒杆菌神经毒素底物复合物鉴定肉毒杆菌神经毒素抑制剂的系统和方法，优选SNAP-25，所述肽底物一侧的报道结构域和所述肽底物相反侧的固定结构域。肉毒杆菌神经毒素抑制剂通过其降低切割复合物的相对量的能力来鉴定，通过测量与固体支持物结合的复合物的降低来检测。本发明的方法还利用表达肉毒神经毒素底物复合物的新细胞。还提供了表达肉毒杆菌毒素底物复合物的新型稳定细胞系和能够在哺乳动物细胞内有效表达BoNT活性轻链的病毒载体。

5. 发明申请

US20120237955A1 METHODS FOR IDENTIFYING INHIBITORS OF BOTULINUM NEUROTOXINS 审中-公开
标题翻译：用于鉴定嗜碱性粒细胞毒素抑制剂的方法
公开(公告)号：US20120237955A1
公开(公告)日：2012-09-20
申请号：US13345691
申请日：2012-01-07
申请人： Randall L. Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
发明人： Randall L. Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
IPC分类号： G01N21/64
CPC分类号： C07K14/705 , C12Q1/37 , G01N33/5014 , G01N2500/04
摘要： A system and method for identifying a botulinum neurotoxin inhibitor employing a botulinum neurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. The botulinum neurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express a botulinum neurotoxin substrate complex. Also provided are novel stable cell lines that express the botulinum toxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.
摘要翻译：一种使用具有肽底物的肉毒杆菌神经毒素底物复合物鉴定肉毒杆菌神经毒素抑制剂的系统和方法，优选SNAP-25，所述肽底物一侧的报道结构域和所述肽底物相反侧的固定结构域。肉毒杆菌神经毒素抑制剂通过其降低切割复合物的相对量的能力来鉴定，通过测量与固体支持物结合的复合物的降低来检测。本发明的方法还利用表达肉毒神经毒素底物复合物的新细胞。还提供了表达肉毒杆菌毒素底物复合物的新型稳定细胞系和能够在哺乳动物细胞内有效表达BoNT活性轻链的病毒载体。

6. 发明授权

US08093044B2 Methods for identifying inhibitors of botulinum neurotoxins 有权
标题翻译：鉴定肉毒杆菌神经毒素抑制剂的方法
公开(公告)号：US08093044B2
公开(公告)日：2012-01-10
申请号：US12630336
申请日：2009-12-03
申请人： Randall Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
发明人： Randall Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
IPC分类号： C07K14/00 , A61K39/08 , C12N5/10
CPC分类号： C07K14/705 , C12Q1/37 , G01N33/5014 , G01N2500/04
摘要： A system and method for identifying a botulinum neurotoxin inhibitor employing a botulinum neurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. The botulinum neurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express a botulinum neurotoxin substrate complex. The methods of the present invention are adapted for cell based screening to monitor the catalytic activity of a BoNT in living cells and to identify molecules that inhibit the catalytic activity of a BoNT in living cells. Also provided are novel stable cell lines that express the botulinum toxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.
摘要翻译：一种使用具有肽底物的肉毒杆菌神经毒素底物复合物鉴定肉毒杆菌神经毒素抑制剂的系统和方法，优选SNAP-25，所述肽底物一侧的报道结构域和所述肽底物相反侧的固定结构域。肉毒杆菌神经毒素抑制剂通过其降低切割复合物的相对量的能力来鉴定，通过测量与固体支持物结合的复合物的降低来检测。本发明的方法还利用表达肉毒神经毒素底物复合物的新细胞。本发明的方法适用于基于细胞的筛选以监测活细胞中BoNT的催化活性并鉴定抑制活细胞中BoNT的催化活性的分子。还提供了表达肉毒杆菌毒素底物复合物的新型稳定细胞系和能够在哺乳动物细胞内有效表达BoNT活性轻链的病毒载体。

7. 发明申请

US20110143362A1 METHOD FOR IDENTIFICATION OF PROTEASE ACTIVITY INHIBITORS AND ASSAYING THE PRESENCE OF PROTEASE ACTIVITY 审中-公开
标题翻译：鉴定蛋白酶活性抑制剂的方法和测定蛋白酶活性的存在
公开(公告)号：US20110143362A1
公开(公告)日：2011-06-16
申请号：US12962610
申请日：2010-12-07
申请人： George Oyler , Yung-Nien Chang , Yien Che Tsai
发明人： George Oyler , Yung-Nien Chang , Yien Che Tsai
IPC分类号： C12Q1/68 , C12N15/63
CPC分类号： C12Q1/37 , C12N15/1034 , C12N15/1086 , C12N15/635 , C12Q1/6897 , C12Q2522/101 , C12Q2521/537
摘要： A system for the identification of proteases and protease inhibitors is provided. The system has at least two components. The first component is a reporter construct with at least one binding site, a transcriptional promoter, an inducible promoter region, and at least one reporter gene, all functionally connected for expression of the reporter gene(s) in functional coordination with a transcriptional activation agent. The second component is a transcriptional activation agent comprising a nucleic acid binding domain, at least one protease substrate domain, and at least one transcriptional activation domain for an inducible promoter. The system allows detection and evaluation of agents affecting protease activity directed to the protease substrate domain. The system also allows for the detection of the presence of proteases in environmental samples.
摘要翻译：提供了用于鉴定蛋白酶和蛋白酶抑制剂的系统。该系统至少有两个组件。第一组分是具有至少一个结合位点，转录启动子，诱导型启动子区和至少一个报告基因的报道构建体，其全部功能连接用于与转录激活剂的功能性配位的报道基因的表达。第二组分是包含核酸结合结构域，至少一个蛋白酶底物结构域和用于诱导型启动子的至少一个转录激活结构域的转录激活剂。该系统允许检测和评估影响针对蛋白酶底物结构域的蛋白酶活性的试剂。该系统还允许检测环境样品中蛋白酶的存在。

8. 发明申请

US20100204054A1 METHODS FOR IDENTIFYING INHIBITORS OF BOTULINUM NEUROTOXINS 有权
标题翻译：用于鉴定嗜碱性粒细胞毒素抑制剂的方法
公开(公告)号：US20100204054A1
公开(公告)日：2010-08-12
申请号：US12630336
申请日：2009-12-03
申请人： Randall L. Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
发明人： Randall L. Kincaid , George Oyler , Yien Che Tsai , Paul S. Fishman
IPC分类号： C40B30/04 , C12N1/20 , G01N33/53
CPC分类号： C07K14/705 , C12Q1/37 , G01N33/5014 , G01N2500/04
摘要： A system and method for identifying a botulinum neurotoxin inhibitor employing a botulinum neurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. The botulinum neurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express a botulinum neurotoxin substrate complex. The methods of the present invention are adapted for cell based screening to monitor the catalytic activity of a BoNT in living cells and to identify molecules that inhibit the catalytic activity of a BoNT in living cells. Also provided are novel stable cell lines that express the botulinum toxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.
摘要翻译：一种使用具有肽底物的肉毒杆菌神经毒素底物复合物鉴定肉毒杆菌神经毒素抑制剂的系统和方法，优选SNAP-25，所述肽底物一侧的报道结构域和所述肽底物相反侧的固定结构域。肉毒杆菌神经毒素抑制剂通过其降低切割复合物的相对量的能力来鉴定，通过测量与固体支持物结合的复合物的降低来检测。本发明的方法还利用表达肉毒神经毒素底物复合物的新细胞。本发明的方法适用于基于细胞的筛选以监测活细胞中BoNT的催化活性并鉴定抑制活细胞中BoNT的催化活性的分子。还提供了表达肉毒杆菌毒素底物复合物的新型稳定细胞系和能够在哺乳动物细胞内有效表达BoNT活性轻链的病毒载体。

9. 发明申请

US20060233836A1 Methods for identifying inhibitors of botulinum neurotoxins 失效
公开(公告)号：US20060233836A1
公开(公告)日：2006-10-19
申请号：US11095055
申请日：2005-03-31
申请人： Randall Kincaid , George Oyler , Yien Tsai , Paul Fishman
发明人： Randall Kincaid , George Oyler , Yien Tsai , Paul Fishman
IPC分类号： A61K39/08 , G01N33/554 , G01N33/569 , C07K14/33
CPC分类号： C07K14/705 , C12Q1/37 , G01N33/5014 , G01N2500/04
摘要： A system and method for identifying a botulinum neurotoxin inhibitor employing a botulinum neurotoxin substrate complex having a peptide substrate, preferably SNAP-25, a reporter domain on one side of said peptide substrate and an immobilization domain on the opposite side of said peptide substrate. The botulinum neurotoxin inhibitor is identified by its ability to decrease the relative amount of cleaved complex, detected through measuring a decrease in complex bound to a solid support. The method of the present invention also utilizes novel cells that express a botulinum neurotoxin substrate complex. The methods of the present invention are adapted for cell based screening to monitor the catalytic activity of a BoNT in living cells and to identify molecules that inhibit the catalytic activity of a BoNT in living cells. Also provided are novel stable cell lines that express the botulinum toxin substrate complex and viral vectors capable of efficiently expressing an active light chain of the BoNT within mammalian cells.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式