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1. 发明申请

US20170240931A1 METHODS AND ORGANISMS FOR THE GROWTH-COUPLED PRODUCTION OF 1,4-BUTANEDIOL 审中-公开
公开(公告)号：US20170240931A1
公开(公告)日：2017-08-24
申请号：US15424723
申请日：2017-02-03
申请人： Genomatica, Inc.
发明人： Anthony P. BURGARD , Stephen J. VAN DIEN , Mark J. BURK
IPC分类号： C12P7/18
CPC分类号： C12P7/18 , B01D3/002 , C12N9/0006
摘要： The invention provides a non-naturally occurring microorganism comprising one or more gene disruptions, the one or more gene disruptions occurring in genes encoding an enzyme obligatory to coupling 1,4-butanediol production to growth of the microorganism when the gene disruption reduces an activity of the enzyme, whereby the one or more gene disruptions confers stable growth-coupled production of 1,4-butanediol onto the non-naturally occurring microorganism. The microorganism can further comprise a gene encoding an enzyme in a 1,4-butanediol (BDO) biosynthetic pathway. The invention additionally relates to methods of using microorganisms to produce BDO.

2. 发明申请

US20150329885A1 MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING ADIPATE, 6-AMINOCAPROATE, HEXAMETHYLENEDIAMINE OR CAPROLACTAM RELATED THERETO 审中-公开
标题翻译：用于提高甲醇存在下降低等效物的可行性的微生物和方法，以及用于生产与其相关的二胺，6-氨基己酸，十六烷二胺或维生素C
公开(公告)号：US20150329885A1
公开(公告)日：2015-11-19
申请号：US14652727
申请日：2013-12-16
申请人： GENOMATICA, INC.
发明人： Anthony P. BURGARD , Robin E. OSTERHOUT , Stephen J. VAN DIEN , Cara Ann TRACEWELL , Priti PHARKYA , Stefan ANDRAE
IPC分类号： C12P17/10 , C12P13/00 , C08G69/08 , C08G63/78 , C08G73/02 , C08G69/16 , C12P7/44 , C12N9/04
CPC分类号： C12P17/10 , A23L29/065 , C08G63/78 , C08G69/08 , C08G69/16 , C08G73/0213 , C12N9/0006 , C12N15/52 , C12P7/24 , C12P7/44 , C12P13/001 , C12P13/005 , C12Y101/01244
摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as adipate, 6-aminocaproate, hexamethylenediamine or caprolactam. Also provided herein are methods for using such an organism to produce adipate, 6-aminocaproate, hexamethylenediamine or caprolactam.
摘要翻译：本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。这样的还原当量可以用于提高微生物生物体如己二酸，6-氨基己酸，六亚甲基二胺或己内酰胺产生的有机化合物的产物产率。本文还提供了使用这种生物体生产己二酸，6-氨基己酸酯，六亚甲基二胺或己内酰胺的方法。

3. 发明申请

US20170362565A1 MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS 审中-公开
公开(公告)号：US20170362565A1
公开(公告)日：2017-12-21
申请号：US15585078
申请日：2017-05-02
申请人： Genomatica, Inc.
发明人： Priti PHARKYA , Anthony P. BURGARD , Stephen J. VAN DIEN , Robin E. OSTERHOUT , Mark J. BURK , John D. TRAWICK , Michael P. KUCHINSKAS , Brian STEER
IPC分类号： C12N1/20 , C12P7/18 , C12N15/63 , C12N15/52 , C12N9/18 , C07C31/20 , C08G63/181 , C08G63/16 , C08G63/06 , C08G18/32 , C08G18/28 , C07C59/01 , C12P7/42 , C08G69/02
CPC分类号： C12N1/20 , C07C31/207 , C07C59/01 , C08G18/284 , C08G18/3206 , C08G63/06 , C08G63/16 , C08G63/181 , C08G69/02 , C12N9/18 , C12N15/52 , C12N15/63 , C12P7/18 , C12P7/42 , C12Y301/01 , Y02P20/52
摘要： The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate or related products using the microbial organisms.

4. 发明申请

US20160326553A1 METHODS AND ORGANISMS WITH INCREASED CARBON FLUX EFFICIENCIES 审中-公开
标题翻译：具有增加碳通量效率的方法和有机体
公开(公告)号：US20160326553A1
公开(公告)日：2016-11-10
申请号：US15108259
申请日：2014-12-23
申请人： GENOMATICA, INC.
发明人： Anthony P. BURGARD , Robin E. OSTERHOUT , Stephen J. VAN DIEN , Priti PHARKYA , Tae Hoon YANG , Jungik CHOI
IPC分类号： C12P7/18 , C12N9/02 , C12N15/52 , C12N9/52 , C12N9/88 , C12N9/16 , C12N9/12
CPC分类号： C12P7/18 , C07K14/245 , C12N1/20 , C12N9/0036 , C12N9/0057 , C12N9/1205 , C12N9/16 , C12N9/52 , C12N9/88 , C12N9/93 , C12N15/52 , C12P7/42 , C12Y106/01002 , C12Y110/03 , C12Y207/0104 , C12Y301/00 , C12Y301/02 , C12Y301/02002 , C12Y304/21092 , C12Y401/01031 , C12Y401/01032
摘要： The invention is directed to a non-naturally occurring microbial organism comprising a first attenuation of a succinyl-CoA synthetase or transferase and at least a second attenuation of a succinyl-CoA converting enzyme or a gene encoding a succinate producing enzyme within a multi-step pathway having a net conversion of succinyl-CoA to succinate.
摘要翻译：本发明涉及一种非天然存在的微生物生物体，其包含琥珀酰-CoA合成酶或转移酶的第一次衰减，以及琥珀酰-CoA转化酶或编码多步骤中产琥珀酸酶的基因的至少第二次衰减途径具有琥珀酰-CoA向琥珀酸盐的净转化。

5. 发明申请

US20170088840A1 COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
公开(公告)号：US20170088840A1
公开(公告)日：2017-03-30
申请号：US15270578
申请日：2016-09-20
申请人： Genomatica, Inc.
发明人： Mark J. BURK , Stephen J. VAN DIEN , Anthony P. BURGARD , Wei NIU
IPC分类号： C12N15/52 , C12N9/04 , C12N15/70 , C12N9/02 , C12N9/00 , C12N15/81 , C12P7/42 , C12N9/88
CPC分类号： C12N15/52 , B01D3/002 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/70 , C12N15/81 , C12P7/18 , C12P7/42 , C12P7/52 , C12P17/04 , C12Y101/01061 , C12Y102/01076 , C12Y401/01071 , C12Y602/01004 , Y02P20/52
摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided are methods for the production of 4-HB and BDO.

6. 发明申请

US20170022524A1 COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
公开(公告)号：US20170022524A1
公开(公告)日：2017-01-26
申请号：US15056914
申请日：2016-02-29
申请人： Genomatica, Inc.
发明人： Mark J. BURK , Stephen J. VAN DIEN , Anthony P. BURGARD , Wei NIU
IPC分类号： C12P7/18 , C12P17/04 , C12N9/88 , C12N9/00 , C12N9/02 , C12P7/42 , C12N9/04
CPC分类号： C12N15/52 , B01D3/002 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/70 , C12N15/81 , C12P7/18 , C12P7/42 , C12P7/52 , C12P17/04 , C12Y101/01061 , C12Y102/01076 , C12Y401/01071 , C12Y602/01004 , Y02P20/52
摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.

7. 发明申请

US20160083752A1 MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, AND FOR PRODUCING SUCCINATE RELATED THERETO 审中-公开
标题翻译：用于提高甲醇存在下减少等效物的可行性的微生物和方法以及用于生产与其相关的成分
公开(公告)号：US20160083752A1
公开(公告)日：2016-03-24
申请号：US14436722
申请日：2013-10-21
申请人： Genomatica, Inc.
发明人： Anthony P. BURGARD , Robin E. OSTERHOUT , Stephen J. VAN DIEN , Cara Ann TRACEWELL , Priti PHARKYA , Stefan ANDRAE
IPC分类号： C12P7/46 , C12P7/24 , C12N15/81 , C12N15/74 , C12N15/77 , C07C59/235 , C12N15/70
CPC分类号： C12P7/46 , C07C55/10 , C07C59/235 , C08G63/00 , C12N9/0006 , C12N9/0028 , C12N9/1007 , C12N15/70 , C12N15/74 , C12N15/77 , C12N15/81 , C12N15/815 , C12P7/24 , C12Y101/01244 , C12Y105/0102
摘要： Provided herein is a non-naturally occurring microbial organism (NNOMO) having a methanol metabolic pathway (MMP) that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as succinate. Also provided herein are methods for using such an organism to produce succinate.
摘要翻译：本文提供了具有甲醇代谢途径（MMP）的非天然存在的微生物生物体（NNOMO），其可以在甲醇存在下增强还原当量的可用性。这种还原当量可以用于增加由微生物生物如琥珀酸酯产生的有机化合物的产物产率。本文还提供了使用这种生物体来生产琥珀酸盐的方法。

8. 发明申请

US20150267229A1 COMPOSITIONS AND METHODS FOR THE BIOSYNTHESIS OF 1,4-BUTANEDIOL AND ITS PRECURSORS 审中-公开
公开(公告)号：US20150267229A1
公开(公告)日：2015-09-24
申请号：US14530530
申请日：2014-10-31
申请人： GENOMATICA, INC.
发明人： Mark J. BURK , Stephen J. VAN DIEN , Anthony P. BURGARD , Wei NIU
IPC分类号： C12P7/42 , C12N9/00 , C12P7/18 , C12N9/88 , C12N9/04 , C12P17/04 , C12N9/02
CPC分类号： C12N15/52 , B01D3/002 , C12N9/0006 , C12N9/0008 , C12N9/88 , C12N9/93 , C12N15/70 , C12N15/81 , C12P7/18 , C12P7/42 , C12P7/52 , C12P17/04 , C12Y101/01061 , C12Y102/01076 , C12Y401/01071 , C12Y602/01004 , Y02P20/52
摘要： The invention provides a non-naturally occurring microbial biocatalyst including a microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway having at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, or α-ketoglutarate decarboxylase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce monomeric 4-hydroxybutanoic acid (4-HB). Also provided is a non-naturally occurring microbial biocatalyst including a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways include at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-butyrate kinase, phosphotransbutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase, wherein the exogenous nucleic acid is expressed in sufficient amounts to produce 1,4-butanediol (BDO). Additionally provided is a method for the production of 4-HB. The method includes culturing a non-naturally occurring microbial organism having a 4-hydroxybutanoic acid (4-HB) biosynthetic pathway including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase or α-ketoglutarate decarboxylase under substantially anaerobic conditions for a sufficient period of time to produce monomeric 4-hydroxybutanoic acid (4-HB). Further provided is a method for the production of BDO. The method includes culturing a non-naturally occurring microbial biocatalyst, comprising a microbial organism having 4-hydroxybutanoic acid (4-HB) and 1,4-butanediol (BDO) biosynthetic pathways, the pathways including at least one exogenous nucleic acid encoding 4-hydroxybutanoate dehydrogenase, succinyl-CoA synthetase, CoA-dependent succinic semialdehyde dehydrogenase, 4-hydroxybutyrate:CoA transferase, 4-hydroxybutyrate kinase, phosphotranshydroxybutyrylase, α-ketoglutarate decarboxylase, aldehyde dehydrogenase, alcohol dehydrogenase or an aldehyde/alcohol dehydrogenase for a sufficient period of time to produce 1,4-butanediol (BDO). The 4-HB and/or BDO products can be secreted into the culture medium.

9. 发明申请

US20170183694A1 MICROORGANISMS AND METHODS FOR PRODUCTION OF 4-HYDROXYBUTYRATE, 1,4-BUTANEDIOL AND RELATED COMPOUNDS 审中-公开
公开(公告)号：US20170183694A1
公开(公告)日：2017-06-29
申请号：US15191421
申请日：2016-06-23
申请人： Genomatica, Inc.
发明人： Priti PHARKYA , Anthony P. BURGARD , Stephen J. VAN DIEN , Robin E. OSTERHOUT , Mark J. BURK , John D. TRAWICK , Michael P. KUCHINSKAS , Brian STEER , Stefan ANDRAE , Amit SHAH
IPC分类号： C12P7/18 , C12P7/42 , C12N1/20 , C12P17/04 , C07C211/09 , C12P13/00 , C07C47/19 , C07C31/20 , C07D307/33 , C12N15/52 , C12N9/10
CPC分类号： C12P7/18 , C07C31/207 , C07C47/19 , C07C211/09 , C07D307/33 , C12N1/20 , C12N9/0006 , C12N9/13 , C12N15/52 , C12P7/24 , C12P7/42 , C12P7/62 , C12P13/001 , C12P13/005 , C12P17/04 , C12P19/32 , C12Y208/03
摘要： The invention provides non-naturally occurring microbial organisms having a 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine pathway and being capable of producing 4-hydroxybutyrate, wherein the microbial organism comprises one or more genetic modifications. The invention additionally provides methods of producing 4-hydroxybutyrate, gamma-butyrolactone, 1,4-butanediol, 4-hydroxybutanal, 4-hydroxybutyryl-CoA and/or putrescine or related products using the microbial organisms.

10. 发明申请

US20170073691A1 MICROORGANISMS AND METHODS FOR ENHANCING THE AVAILABILITY OF REDUCING EQUIVALENTS IN THE PRESENCE OF METHANOL, FOR PRODUCING METHACRYLIC ACID 审中-公开
标题翻译：用于提高甲醇存在下生产甲基丙酸的减少等效物的可行性的微生物和方法
公开(公告)号：US20170073691A1
公开(公告)日：2017-03-16
申请号：US15133150
申请日：2016-04-19
申请人： Genomatica, Inc.
发明人： Anthony P. BURGARD , Robin E. OSTERHOUT , Stephen J. VAN DIEN , Cara Ann TRACEWELL , Priti PHARKYA , Stefan ANDRAE
IPC分类号： C12N15/52 , C12N9/10 , C12N9/06 , C12P7/62 , C12N9/04
CPC分类号： C12N15/52 , C08F120/06 , C08G63/664 , C12N9/0006 , C12N9/0028 , C12N9/1007 , C12N15/63 , C12P7/24 , C12P7/40 , C12P7/42 , C12P7/62 , C12Y101/01244 , C12Y105/0102 , C12Y201/0109
摘要： Provided herein is a non-naturally occurring microbial organism having a methanol metabolic pathway that can enhance the availability of reducing equivalents in the presence of methanol. Such reducing equivalents can be used to increase the product yield of organic compounds produced by the microbial organism, such as 3-hydroxyisobutyrate or MAA. Also provided herein are methods for using such an organism to produce 3-hydroxyisobutyrate or MAA.
摘要翻译：本文提供了具有甲醇代谢途径的非天然存在的微生物生物，其可以在甲醇存在下增强还原当量的可用性。这种还原当量可用于增加由微生物生物体如3-羟基异丁酸酯或MAA产生的有机化合物的产物产率。本文还提供了使用这种生物体产生3-羟基异丁酸酯或MAA的方法。

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