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1. 发明申请

US20080163398A1 GENES AND VECTORS FOR CONFERRING HERBICIDE RESISTANCE IN PLANTS 失效
标题翻译：在植物中鉴定抗除草剂的基因和载体
公开(公告)号：US20080163398A1
公开(公告)日：2008-07-03
申请号：US12027011
申请日：2008-02-06
申请人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
发明人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
IPC分类号： A01H5/00 , C07H21/04 , C12N15/82 , C12N5/10 , C12N15/63
CPC分类号： C12N15/8278 , C12N9/88
摘要： Genomic and cDNA sequences and plant expression vectors encoding for an eukaryotic AHAS small subunit protein are disclosed. The DNA sequences and vectors are used to transform plants to produce transgenic plants which possess elevated levels of tolerance or resistance to herbicides, such as imidazolinones.
摘要翻译：公开了编码真核AHAS小亚基蛋白的基因组和cDNA序列和植物表达载体。 DNA序列和载体用于转化植物以产生对除草剂如咪唑啉酮具有较高耐受性或抗性的转基因植物。

2. 发明授权

US07351880B2 Genes and vectors for conferring herbicide resistance in plants 失效
标题翻译：在植物中赋予除草剂抗性的基因和载体
公开(公告)号：US07351880B2
公开(公告)日：2008-04-01
申请号：US10970515
申请日：2004-10-21
申请人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
发明人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
IPC分类号： C12N15/29 , C12N15/82 , A01H5/00
CPC分类号： C12N15/8278 , C12N9/88
摘要： Genomic and cDNA sequences and plant expression vectors encoding for an eukaryotic AHAS small subunit protein are disclosed. The DNA sequences and vectors are used to transform plants to produce transgenic plants which possess elevated levels of tolerance or resistance to herbicides, such as imidazolinones.
摘要翻译：公开了编码真核AHAS小亚基蛋白的基因组和cDNA序列和植物表达载体。 DNA序列和载体用于转化植物以产生对除草剂如咪唑啉酮具有较高耐受性或抗性的转基因植物。

3. 发明申请

US20050108787A1 Genes and vectors for conferring herbicide resistance in plants 失效
标题翻译：在植物中赋予除草剂抗性的基因和载体
公开(公告)号：US20050108787A1
公开(公告)日：2005-05-19
申请号：US10970515
申请日：2004-10-21
申请人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
发明人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
IPC分类号： A01H5/00 , A01N63/00 , C12N5/10 , C12N9/88 , C12N15/09 , C12N15/29 , C12N15/60 , C12N15/82 , C12R1/91 , A01H1/00 , C12N5/04 , C12Q1/68
CPC分类号： C12N15/8278 , C12N9/88
摘要： Genomic and cDNA sequences and plant expression vectors encoding for an eukaryotic AHAS small subunit protein are disclosed. The DNA sequences and vectors are used to transform plants to produce transgenic plants which possess elevated levels of tolerance or resistance to herbicides, such as imidazolinones.
摘要翻译：公开了编码真核AHAS小亚基蛋白的基因组和cDNA序列和植物表达载体。 DNA序列和载体用于转化植物以产生对除草剂如咪唑啉酮具有较高耐受性或抗性的转基因植物。

4. 发明授权

US07498429B2 AHAS small subunit promoter 失效
标题翻译： AHAS小亚基启动子
公开(公告)号：US07498429B2
公开(公告)日：2009-03-03
申请号：US12027011
申请日：2008-02-06
申请人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
发明人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
IPC分类号： C12N15/11 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8278 , C12N9/88
摘要： A promoter sequence and plant expression vectors encoding for an eukaryotic AHAS small subunit protein are disclosed. The DNA sequences and vectors are used to transform plants to produce transgenic plants which possess elevated levels of tolerance or resistance to herbicides, such as imidazolinones.
摘要翻译：公开了编码真核AHAS小亚基蛋白的启动子序列和植物表达载体。 DNA序列和载体用于转化植物以产生对除草剂如咪唑啉酮具有较高耐受性或抗性的转基因植物。

5. 发明授权

US06825399B2 Genes and vectors for conferring herbicide resistance in plants 失效
标题翻译：在植物中赋予除草剂抗性的基因和载体
公开(公告)号：US06825399B2
公开(公告)日：2004-11-30
申请号：US09997900
申请日：2001-11-30
申请人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
发明人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
IPC分类号： A01H500
CPC分类号： C12N15/8278 , C12N9/88
摘要： Genomic and cDNA sequences and plant expression vectors for encoding an eukaryotic AHAS small subunit are disclosed. The DNA sequences and vectors are used to transform plants to produce transgenic plants which possess elevated levels of tolerance or resistance to herbicides, such as imidazolinones.
摘要翻译：公开了用于编码真核AHAS小亚基的基因组和cDNA序列和植物表达载体。 DNA序列和载体用于转化植物以产生对除草剂如咪唑啉酮具有较高耐受性或抗性的转基因植物。

6. 发明授权

US06348643B1 DNA sequences encoding the arabidopsis acetohydroxy-acid synthase small subunit and methods of use 失效
标题翻译：编码拟南芥乙酰羟酸合酶小亚基的DNA序列及其使用方法
公开(公告)号：US06348643B1
公开(公告)日：2002-02-19
申请号：US09426568
申请日：1999-10-22
申请人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
发明人： Genichi Kakefuda , Colleen Costello , Ming Sun , Weiming Hu
IPC分类号： C12N1560
CPC分类号： C12N15/8278 , C12N9/88
摘要： Genomic and cDNA sequences and plant expression vectors encoding for an arabidopsis AHAS small subunit protein are disclosed. The DNA sequences and vectors are used to transform plants to produce transgenic plants which possess elevated levels of tolerance or resistance to herbicides, such as imidazolinones.
摘要翻译：公开了编码拟南芥AHAS小亚基蛋白的基因组和cDNA序列和植物表达载体。 DNA序列和载体用于转化植物以产生对除草剂如咪唑啉酮具有较高耐受性或抗性的转基因植物。

7. 发明申请

US20070250946A1 Monocot Ahass Sequences and Methods of Use 审中-公开
标题翻译： Monocot Ahass序列和使用方法
公开(公告)号：US20070250946A1
公开(公告)日：2007-10-25
申请号：US11659129
申请日：2005-08-04
申请人： Robert Ascenzi , Gregory Budziszewski , Genichi Kakefuda , Bijay Singh
发明人： Robert Ascenzi , Gregory Budziszewski , Genichi Kakefuda , Bijay Singh
IPC分类号： A01H1/00 , A01H5/00 , A01N43/50 , C07H21/04 , C07K1/00 , C12N15/00 , C12N5/00
CPC分类号： C12Y202/01006 , C12N9/88 , C12N15/8274 , C12N15/8278
摘要： Isolated polynucleotides that encode acetohydroxyacid synthase small subunit (AHASS) polypeptides, and the amino acid sequences encoding these polypeptides, are described. Expression cassettes and expression vectors comprising the polynucleotides of the invention, as well as plants and host cells transformed with the polynucleotides, expression cassettes, and expression vectors, are described. Methods of using the polynucleotides to enhance the resistance of plants to herbicides are also described.
摘要翻译：描述了编码乙酰羟酸合酶小亚基（AHASS）多肽的分离的多核苷酸，以及编码这些多肽的氨基酸序列。描述了包含本发明的多核苷酸的表达盒和表达载体，以及用多核苷酸，表达盒和表达载体转化的植物和宿主细胞。还描述了使用多核苷酸增强植物对除草剂的抗性的方法。

8. 发明授权

US07083967B1 Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation 失效
标题翻译：编码可用于通过核或plastome转化来控制植物性状的蛋白质的蓝细菌核酸片段
公开(公告)号：US07083967B1
公开(公告)日：2006-08-01
申请号：US09893033
申请日：2001-06-27
申请人： Genichi Kakefuda , Hans Koop , Stephen Sturner , Rui-Guang Zhen
发明人： Genichi Kakefuda , Hans Koop , Stephen Sturner , Rui-Guang Zhen
IPC分类号： C12N9/88 , C12N1/20 , C12N15/00 , C07H21/04 , A01H1/00
CPC分类号： C12N15/8278 , C12N9/0004 , C12N9/88 , C12Y202/01006
摘要： This invention provides an alternative source of ahas and pds nucleic acids for plant transformation and selection. In particular, the invention provides ahas and pds nucleic acids from cyanobacteria, for example, Synechocystis, and expression elements of these genes for control of expression in plastids. The invention further provides nucleic acids encoding the acetolactate synthase (ahas) large subunit and the ahas small subunit which were found to provide herbicide resistance to plants. The present invention also provides a novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6-[(alpha, alpha, alpha,-trfluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improved methods involving cyanobacteria for the screening of compounds, including a new high throughput protocol that is a rapid and cost effective way to identify target site genes.
摘要翻译：本发明提供用于植物转化和选择的ahas和pds核酸的替代来源。特别地，本发明提供了来自蓝细菌的Ahas和pds核酸，例如集胞藻，以及用于控制质体中表达的这些基因的表达元件。本发明还提供了编码乙酰乳酸合酶（ahas）大亚基的核酸和被发现提供对植物的除草剂抗性的小亚单位。本发明还提供了赋予对4'-氟-6 - [（α，α，α， - 氟 - 间甲苯基）氧基] - 甲酰胺酰胺（漂白除草剂）的抗性的新颖的集胞藻突变体植物衍生物去饱和酶（PDS）基因。本发明提供了涉及用于筛选化合物的蓝细菌的改进方法，包括新的高通量方案，其是识别靶位点基因的快速和成本有效的方法。

9. 发明授权

US07285701B2 Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation 有权
标题翻译：编码可用于通过核或plastome转化来控制植物性状的蛋白质的蓝细菌核酸片段
公开(公告)号：US07285701B2
公开(公告)日：2007-10-23
申请号：US11244732
申请日：2005-10-06
申请人： Genichi Kakefuda , Rui-Guang Zhen
发明人： Genichi Kakefuda , Rui-Guang Zhen
IPC分类号： A01H1/00 , C12N9/00 , C12N15/00
CPC分类号： C07K14/195 , C12Q1/689
摘要： This invention provides cyanobacteria as an alternative source of ahas and pds genes for plant transformations and for selectable markers. In particular, it provides for cyanobacteria, for example, Synechocystis, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (ahas) large subunit and the ahas small subunit, were found to provide herbicide resistance. Also, the present invention provides novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6[(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes.
摘要翻译：本发明提供蓝藻作为植物转化和可选择标记的ahas和pds基因的替代来源。特别地，其提供蓝细菌，例如集胞藻，作为编码除草剂不敏感蛋白的基因的来源，以及用于控制质体中表达的基因的元件。发现核酸片段，乙酰乳酸合酶（ahas）大亚基和ahas小亚单位，提供除草剂抗性。此外，本发明提供了赋予对4'-氟-6 [（α，α，α， - 三氟 - 间甲苯基）氧基] - 甲酰胺酰胺（漂白除草剂）的抗性的新颖的集胞藻突变体八氢番茄红素去饱和酶（PDS）基因。本发明提供了涉及用于筛选化合物的蓝细菌的方法的改进，包括新的高通量方案，其是鉴定靶位点基因的快速和成本有效的方法。

10. 发明申请

US20060156427A1 Structure-based designed herbicide resistant products 审中-公开
公开(公告)号：US20060156427A1
公开(公告)日：2006-07-13
申请号：US11033687
申请日：2005-01-12
申请人： Genichi Kakefuda , Karl-Heinz Ott , Jae-Gyu Kwagh , Gerald Stockton
发明人： Genichi Kakefuda , Karl-Heinz Ott , Jae-Gyu Kwagh , Gerald Stockton
IPC分类号： C12N15/82 , G06G7/48 , G06G7/58 , A01H1/00
CPC分类号： C12N9/88 , C12N15/8278 , G16B15/00
摘要： Disclosed herein are structure-based modelling methods for the preparation of acetohydroxy acid synthase (AHAS) variants, including those that exhibit selectively increased resistance to herbicides such as imidazoline herbicides and AHAS inhibiting herbicides. The invention encompasses isolated DNAs encoding such variants, vectors that include the DNAs, and methods for producing the variant polypeptides and herbicide resistant plants containing specific AHAS gene mutations. Methods for weed control in crops are also provided.

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