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    • 6. 发明授权
    • Cyanobacterial nucleic acid fragments encoding proteins useful for controlling plant traits via nuclear or plastome transformation
    • 编码可用于通过核或plastome转化来控制植物性状的蛋白质的蓝细菌核酸片段
    • US07285701B2
    • 2007-10-23
    • US11244732
    • 2005-10-06
    • Genichi KakefudaRui-Guang Zhen
    • Genichi KakefudaRui-Guang Zhen
    • A01H1/00C12N9/00C12N15/00
    • C07K14/195C12Q1/689
    • This invention provides cyanobacteria as an alternative source of ahas and pds genes for plant transformations and for selectable markers. In particular, it provides for cyanobacteria, for example, Synechocystis, as a source of genes encoding herbicide insensitive proteins, and elements of genes for control of expression in plastids. Nucleic acid fragments, both the acetolactate synthase (ahas) large subunit and the ahas small subunit, were found to provide herbicide resistance. Also, the present invention provides novel Synechocystis mutant phytoene desaturase (PDS) gene conferring resistance to 4′-fluoro-6[(alpha,alpha,alpha,-trifluoro-m-tolyl)oxy]-picolinamide, a bleaching herbicide. The present invention provides improvements to method involving cyanobacteria for the screening of compounds, including a new high-through-put protocol that is a rapid and cost effective way to identify target site genes.
    • 本发明提供蓝藻作为植物转化和可选择标记的ahas和pds基因的替代来源。 特别地,其提供蓝细菌,例如集胞藻,作为编码除草剂不敏感蛋白的基因的来源,以及用于控制质体中表达的基因的元件。 发现核酸片段,乙酰乳酸合酶(ahas)大亚基和ahas小亚单位,提供除草剂抗性。 此外,本发明提供了赋予对4'-氟-6 [(α,α,α, - 三氟 - 间甲苯基)氧基] - 甲酰胺酰胺(漂白除草剂)的抗性的新颖的集胞藻突变体八氢番茄红素去饱和酶(PDS)基因。 本发明提供了涉及用于筛选化合物的蓝细菌的方法的改进,包括新的高通量方案,其是鉴定靶位点基因的快速和成本有效的方法。