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1. 发明申请

US20180057791A1 METHODS AND KITS FOR CELL ACTIVATION 审中-公开
公开(公告)号：US20180057791A1
公开(公告)日：2018-03-01
申请号：US15245584
申请日：2016-08-24
申请人： General Electric Company
发明人： Ernest William Kovacs , Anup Sood , Reginald Donovan Smith , Evelina Roxana Loghin , Padmaparna Chadhuri , Vandana Keskar , Chrystal Mae Chadwick , Martin James Brown
IPC分类号： C12N5/0783 , C07K16/28
CPC分类号： C12N5/0636 , C07K16/2809 , C07K16/2818 , C12N2501/51 , C12N2501/515
摘要： Provided are methods and kits for activating T cells, the method comprising providing a population of T cells, adding a plurality of first agents, where the first agent comprises a T-cell activator and a first binder moiety, and adding a second agent comprising a plurality of capture oligomers, where at least a segment of at least one of the plurality of capture oligomers is capable of associating with the first binder moiety. The method further comprises incubating the population of T cells, whereby at least a portion of the population of T cells is activated.

2. 发明申请

US20160376550A1 CELL CULTURE SUPPORT AND ASSOCIATED METHOD FOR CELL GROWTH AND RELEASE 审中-公开
标题翻译：细胞培养支持和细胞生长和释放的相关方法
公开(公告)号：US20160376550A1
公开(公告)日：2016-12-29
申请号：US15262644
申请日：2016-09-12
申请人： General Electric Company
发明人： Reginald Donovan Smith , Prameela Susarla , Slawomir Rubinsztajn , Brian David Polizzotti , Anton Beletskii
IPC分类号： C12N5/00 , C12M1/26 , C08F8/12 , C12M1/00
CPC分类号： C12N5/0075 , C08F8/12 , C08F293/005 , C12M23/20 , C12M33/00 , C12N5/0068 , C12N2533/30 , C12N2539/10
摘要： A cell culture support comprising a substrate, and a dual stimuli responsive block copolymer immobilized on the substrate, wherein the dual stimuli responsive block copolymer is both thermoresponsive and pH responsive. A method of culturing cells comprising the cell culture support having a dual stimuli responsive copolymer immobilized on a substrate, wherein the dual stimuli responsive copolymer is thermoresponsive and pH responsive; and growing the cells on the cell culture support. By lowering the temperature, cells are released from the cell culture support.
摘要翻译：一种细胞培养支持物，其包含底物和固定在底物上的双重刺激响应性嵌段共聚物，其中所述双重刺激响应性嵌段共聚物是热反应性和pH响应性的。一种培养细胞的方法，所述细胞包含具有固定在底物上的双重刺激响应共聚物的细胞培养支持物，其中所述双重刺激响应共聚物是热反应性和pH响应性的; 并在细胞培养支持下培养细胞。通过降低温度，细胞从细胞培养支持物释放。

3. 发明授权

US09677975B2 Systems and methods for aseptic sampling 有权
公开(公告)号：US09677975B2
公开(公告)日：2017-06-13
申请号：US14529203
申请日：2014-10-31
申请人： General Electric Company
发明人： Chengkun Zhang , Kenneth Roger Conway , Donald Joseph Buckley , Eugene Pauling Boden , Weston Blaine Griffin , Anshika Bajaj , Reginald Donovan Smith
IPC分类号： G01N1/18 , C12M1/00 , C12M1/32 , C12M1/26 , C12M1/12 , C12M1/34
CPC分类号： G01N1/18 , C12M23/00 , C12M23/40 , C12M33/00 , C12M33/06 , C12M37/02 , C12M41/00
摘要： A sampling assembly configured to be coupled to a sample source and facilitate aseptic sampling at one or more instances in time is provided. Further, the sampling assembly includes a first conduit having first and second ports, where the first port is configured to be coupled to the sample source. The sampling assembly also includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connector junctions. Also, each of the sub-ports is in fluidic communication with the first conduit. The sampling assembly also includes a plurality of sampling kits and one or more pumping devices. Further, each sampling kit is operatively coupled to a respective sub-port of a corresponding sub-conduit. Moreover, the one or more pumping devices are operatively and aseptically coupled to the second port of the first conduit.

4. 发明申请

US20160122798A1 SYSTEMS AND METHODS FOR ASEPTIC SAMPLING 有权
标题翻译：用于自由采样的系统和方法
公开(公告)号：US20160122798A1
公开(公告)日：2016-05-05
申请号：US14529203
申请日：2014-10-31
申请人： General Electric Company
发明人： Chengkun Zhang , Kenneth Roger Conway , Donald Joseph Buckley , Eugene Pauling Boden , Weston Blaine Griffin , Anshika Bajaj , Reginald Donovan Smith
IPC分类号： C12Q1/24 , G01N1/14
CPC分类号： G01N1/18 , C12M23/00 , C12M23/40 , C12M33/00 , C12M33/06 , C12M37/02 , C12M41/00
摘要： A sampling assembly configured to be coupled to a sample source and facilitate aseptic sampling at one or more instances in time is provided. Further, the sampling assembly includes a first conduit having first and second ports, where the first port is configured to be coupled to the sample source. The sampling assembly also includes a plurality of sub-conduits having corresponding sub-ports, where each of the plurality of sub-conduits is operatively coupled to the first conduit at respective connector junctions. Also, each of the sub-ports is in fluidic communication with the first conduit. The sampling assembly also includes a plurality of sampling kits and one or more pumping devices. Further, each sampling kit is operatively coupled to a respective sub-port of a corresponding sub-conduit. Moreover, the one or more pumping devices are operatively and aseptically coupled to the second port of the first conduit.
摘要翻译：提供了一种采样组件，其被配置为耦合到采样源并且在一个或多个时间上促进无菌采样。此外，采样组件包括具有第一和第二端口的第一导管，其中第一端口被配置为耦合到采样源。采样组件还包括具有对应的子端口的多个子管道，其中多个子导管中的每一个在相应的连接器接合处可操作地联接到第一导管。此外，每个子端口与第一导管流体连通。采样组件还包括多个采样套件和一个或多个抽吸装置。此外，每个采样套件可操作地耦合到相应子导管的相应子端口。此外，一个或多个泵送装置可操作地和无菌地联接到第一管道的第二端口。

5. 发明申请

US20150299657A1 GAMMA STABILIZED DEXTRAN SOLUTIONS AND METHODS OF USE 审中-公开
标题翻译： GAMMA稳定的德克萨斯解决方案和使用方法
公开(公告)号：US20150299657A1
公开(公告)日：2015-10-22
申请号：US14254152
申请日：2014-04-16
申请人： GENERAL ELECTRIC COMPANY
发明人： Patrick Joseph McCloskey , James Edward Pickett , Gregory Daryll Goddard , Reginald Donovan Smith , Anshika Bajaj
IPC分类号： C12N5/078
CPC分类号： C12N5/0087 , C12N2500/50 , G01N33/491
摘要： Provided herein are gamma stable dextran solutions and kits that increase the efficiency of blood separation by allowing the dextran solution to be sterilized by exposure to gamma radiation and maintain sufficient molecular weight to act as a red blood cell aggregant. Further provided are methods of use.
摘要翻译：本文提供了γ稳定的葡聚糖溶液和试剂盒，其通过使葡聚糖溶液通过暴露于γ辐射而被消毒并且保持足够的分子量以充当红细胞聚集体来提高血液分离的效率。进一步提供使用的方法。

6. 发明申请

US20130171729A1 METHODS AND KITS FOR CELL RELEASE 有权
标题翻译：细胞释放的方法和工具
公开(公告)号：US20130171729A1
公开(公告)日：2013-07-04
申请号：US13776053
申请日：2013-02-25
申请人： GENERAL ELECTRIC COMPANY
发明人： Slawomir Rubinsztajn , Prameela Susarla , Reginald Donovan Smith , Evelina Roxana Loghin
IPC分类号： C12N5/00
CPC分类号： C12N5/0068 , C12M23/20 , C12M33/00 , C12N2533/32 , C12N2539/10
摘要： Methods and kits of releasing cells are provided. The method comprises the steps of providing cultured cells on a cell culture support comprising a multi layer polyelectrolyte coating immobilized on a substrate, and releasing the cultured cells from the cell culture support by a releasing solution comprising DMSO. The kit comprises a cell culture support and a releasing solution. The releasing solution comprises DMSO.
摘要翻译：提供了释放细胞的方法和试剂盒。该方法包括以下步骤：在包含固定在底物上的多层聚电解质涂层的细胞培养载体上提供培养的细胞，以及通过包含DMSO的释放溶液从细胞培养支持物释放培养的细胞。该试剂盒包括细胞培养支持物和释放溶液。释放溶液包含DMSO。

7. 发明申请

US20190249130A1 CELL EXPANSION VESSEL SYSTEMS AND METHODS 审中-公开
公开(公告)号：US20190249130A1
公开(公告)日：2019-08-15
申请号：US15893336
申请日：2018-02-09
申请人： General Electric Company
发明人： Weston Blaine Griffin , Xiaohua Zhang , Reginald Donovan Smith , Vandana Keskar
IPC分类号： C12M1/00 , C12N5/0783 , C12M1/04 , C12M1/12 , C12M1/26 , C12M1/34
CPC分类号： C12M29/04 , C12M23/24 , C12M25/02 , C12M25/06 , C12M29/26 , C12M33/00 , C12M41/46 , C12N5/0636
摘要： A method includes introducing a suspension including cells suspended in a cell culture medium through a feed port or a drain port into a cavity of a cell culture vessel, the suspension being in an amount sufficient to cover a gas permeable, liquid impermeable membrane positioned at a bottom of the cell culture vessel, the feed port being disposed through a surface of the cell culture vessel and configured to permit additional cell culture medium into the cavity, and the drain port being disposed through the surface of the cell culture vessel and configured to permit removal of the cells, cell culture medium, and used cell culture medium from the cavity, allowing the cells to settle on the gas permeable, liquid impermeable membrane by gravity, removing the used cell culture medium through the drain port and introducing the additional cell culture medium through the feed port such that a constant volume is maintained in the cell culture vessel until the cells expand to a desired cell density, wherein the removing and introducing are performed subsequent to allowing the cells to settle on the gas permeable, liquid impermeable membrane, resuspending the cells in the cell culture medium in the cell culture vessel, wherein the resuspending is performed after the desired cell density is attained, and removing the resuspended cells and the cell culture medium through the drain port.

8. 发明授权

US09587215B2 Devices, systems and methods for automated transfer of a sample 有权
标题翻译：用于自动传送样品的设备，系统和方法
公开(公告)号：US09587215B2
公开(公告)日：2017-03-07
申请号：US14453683
申请日：2014-08-07
申请人： General Electric Company
发明人： Ying Mao , Kenneth Roger Conway , Weston Blaine Griffin , Reginald Donovan Smith , Evelina Roxana Loghin , Vandana Keskar , Chengkun Zhang , Zhipeng Zhang
IPC分类号： C12M1/26 , C12M1/34 , A01N1/02 , B01L7/00 , C12M1/00
CPC分类号： C12M33/00 , A01N1/0236 , A01N1/0268 , B01L7/00 , B01L2300/1827 , C12M1/26 , C12M33/04 , C12M41/12 , C12M45/20 , C12M45/22
摘要： A coupling device configured to form a sample access assembly is provided. The sample access assembly is configured to house a sample. The coupling device includes a heating component and a separating component. Further, the separating component is configured to separate portions of first and second containers that form first and second compartments of the sample access assembly. Moreover, the heating component is configured to heat at least a portion of the sample.
摘要翻译：提供了构造成形成样品存取组件的联接装置。样品存取组件被配置为容纳样品。联接装置包括加热部件和分离部件。此外，分离部件构造成分离形成样品进入组件的第一和第二隔室的第一和第二容器的部分。此外，加热部件构造成加热样品的至少一部分。

9. 发明申请

US20170058252A1 SYSTEM AND METHOD FOR INITIATING A CELL CULTURE 有权
标题翻译：用于启动细胞培养的系统和方法
公开(公告)号：US20170058252A1
公开(公告)日：2017-03-02
申请号：US14841072
申请日：2015-08-31
申请人： General Electric Company
发明人： Chengkun Zhang , Reginald Donovan Smith , Kenneth Roger Conway , Anshika Bajaj
IPC分类号： C12M1/02 , C12M3/00 , C12M1/00 , C12Q3/00
CPC分类号： C12M41/22 , C12M21/08 , C12M23/14 , C12M23/38 , C12M23/48 , C12M27/16 , C12M29/24 , C12M41/48 , C12Q3/00
摘要： System and methods are provided for initiating a cell culture. The systems and methods include a first enclosure containing a thermal mass positioned on a surface area of a platform. The systems and methods include a second enclosure, containing a culture fluid and a gas mixture, placed on the first enclosure such that the first enclosure is positioned between the second enclosure and the platform. The systems and methods further include a cover secured on the second enclosure, and generating first thermal energy on the surface area of the platform and second thermal energy from the cover.
摘要翻译：提供了用于启动细胞培养的系统和方法。该系统和方法包括一个包含位于平台表面区域上的热质量的第一外壳。所述系统和方法包括放置在第一外壳上的包含培养液和气体混合物的第二外壳，使得第一外壳位于第二外壳和平台之间。所述系统和方法还包括固定在第二外壳上的盖，并且在平台的表面区域上产生第一热能并且从盖产生第二热能。

10. 发明申请

US20160123848A1 SEMI-AUTOMATED SAMPLING SYSTEM FOR ASEPTIC SAMPLING 审中-公开
标题翻译：用于自动采样的半自动采样系统
公开(公告)号：US20160123848A1
公开(公告)日：2016-05-05
申请号：US14529201
申请日：2014-10-31
申请人： General Electric Company
发明人： Weston Blaine Griffin , Chengkun Zhang , Kenneth Roger Conway , Reginald Donovan Smith
IPC分类号： G01N1/14 , G01N33/48 , G01N35/10
CPC分类号： G01N1/14 , C12M29/12 , C12M33/06 , C12M37/02 , C12M41/00
摘要： A semi-automated sampling assembly configured for aseptic sampling at one or more instances from a sample source having a biological inoculum is provided. The semi-automated sampling assembly includes a sampling conduit, a recovery conduit, one or more sampling kits, and a pumping device. The sampling conduit includes a first port and a second port, where the first port of the sampling conduit is configured to be operatively coupled to the sample source. Further, the recovery conduit includes a first port and a second port, where the first port of the recovery conduit is configured to be operatively coupled to the sample source. Also, the second port of the recovery conduit is operatively coupled to at least a portion of the sampling conduit. Moreover, the one or more sampling kits are operatively coupled to the sampling conduit, and the pumping device is operatively coupled to the sampling conduit.
摘要翻译：提供了一种半自动采样组件，其被配置为在具有生物接种物的样品源的一个或多个实例处进行无菌取样。半自动采样组件包括采样导管，回收管道，一个或多个采样套件以及泵送装置。采样导管包括第一端口和第二端口，其中采样导管的第一端口被配置为可操作地耦合到采样源。此外，回收管道包括第一端口和第二端口，其中回收管道的第一端口被配置为可操作地耦合到采样源。此外，回收管道的第二端口可操作地联接到采样管道的至少一部分。此外，一个或多个采样套件可操作地耦合到采样导管，并且泵送装置可操作地耦合到采样导管。

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