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    • 1. 发明授权
    • Positioning method, positioning apparatus and sequencing system
    • US11421272B2
    • 2022-08-23
    • US17417035
    • 2019-09-29
    • GeneMind Biosciences Company Limited
    • Ruitao SunSongzhen ZhangZefei JiangZhiliang ZhouQin Yan
    • C12Q1/6869G01N21/01G02B21/26G03B5/00B01J19/18G01N35/00
    • Disclosed are a positioning method, a positioning apparatus and a sequencing system (300). The sequencing system (300) comprises an imaging assembly (110) and a mobile platform (102), wherein the imaging assembly (110) comprises an optical axis (OP), the mobile platform (102) bears a reaction apparatus (200), the stroke of the mobile platform (102) in a first direction is controlled by means of a limiting switch, and the stroke comprises a first limit. The method comprises the steps: (a) moving, by a first step distance, the mobile platform (102) in a direction approaching the first limit, until the distance between a current position of the reaction apparatus (200) and the previous position of the reaction apparatus (200) is less than the first step distance; (b) moving the mobile platform (102) in a direction that is the opposite of the moving direction in step (a); (c) moving the mobile platform (102) in the moving direction in step (a), until the mobile platform (102) reaches the first limit; and (d) moving, by a preset distance, the mobile platform (102) in the direction that is the opposite of the moving direction in step (a), so that the positions of the optical axis (OP) and the reaction apparatus (200) satisfy a preset relationship. According to the method, the quick and precise positioning of the mobile platform (102) can be realized by means of moving back and forth in the first direction, such that the positions of the optical axis (OP) and the reaction apparatus (200) satisfy a preset relationship.
    • 3. 发明授权
    • Alignment methods, devices and systems
    • US11482304B2
    • 2022-10-25
    • US16635996
    • 2017-08-02
    • GENEMIND BIOSCIENCES COMPANY LIMITED
    • Weibin XuHuan JinQin YanZefei JiangZhiliang Zhou
    • G01N33/48G16B30/10G16B35/10
    • The disclosure discloses an alignment method, device, and system. The alignment method includes: converting each read into a set of short fragments corresponding to the read to obtain a plurality of sets of short fragments; determining a corresponding position of the short fragment in a reference library to obtain a first positioning result, wherein the reference library is a hash table constructed based on a reference sequence, the reference library includes a plurality of entries, one entry of the reference library corresponds to one seed sequence, and the seed sequence is capable of matching at least one sequence on the reference sequence, a distance between two seed sequences corresponding to two adjacent entries of the reference library on the reference sequence is less than a length of the short fragment; removing a short fragment positioned on any one of the adjacent entries of the reference library in the first positioning result to obtain a second positioning result; and extending based on short fragments from the same read in the second positioning result to obtain an alignment result of the read. The alignment method can efficiently and accurately process and position sequencing data.