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1. 发明授权

US4971671A Processes for separation of DNA fragments 失效
标题翻译：分离DNA片段的方法
公开(公告)号：US4971671A
公开(公告)日：1990-11-20
申请号：US20401
申请日：1987-03-02
申请人： Gary W. Slater , Jaan Noolandi
发明人： Gary W. Slater , Jaan Noolandi
IPC分类号： B01D57/02 , C12Q1/68 , G01N27/447
CPC分类号： C12Q1/68 , B01D57/02 , G01N27/44773
摘要： A process for enabling the separation of a mixture of DNA fragments comprising (1) providing an electrophoresis devive; (2) adding to the device a solution mixture containing DNA fragments of different lengths; (3) energizing the device, thereby creating a sequence of unidirectional uniform electric field pulses therein, said sequence of field pulses alternating between primary positive voltage pulses and secondary pulses of a negative or a positive polarity with less voltage than the primary pulses; (4) calculating the time duration and the field strength required for the primary and secondary field pulses to enable resolution of the fragments into separate and distinct groups corresponding their lengths; and (5) applying in the device the selected primary and secondary fields with intensities and durations corresponding to the size of fragments to be separated. Another embodiment of the invention comprises a computer program that facilitates a DNA gel electrophoresis process.
摘要翻译：一种能够分离DNA片段的混合物的方法，包括（1）提供电泳变体; （2）向装置中加入含有不同长度的DNA片段的溶液混合物; （3）给器件通电，由此在其中产生单向均匀电场脉冲序列，所述场脉冲序列在初级正电压脉冲与负脉冲或正极性的次级脉冲之间交替，电压小于主脉冲; （4）计算一次和二次场脉冲所需的持续时间和场强使得能够将片段分解成与其长度对应的分离和不同组; 以及（5）在所述设备中应用所选择的一次和二次场，其强度和持续时间对应于要分离的片段的大小。本发明的另一个实施方案包括促进DNA凝胶电泳过程的计算机程序。

2. 发明授权

US5544890A Golf ball dimple patterns 失效
标题翻译：高尔夫球凹坑图案
公开(公告)号：US5544890A
公开(公告)日：1996-08-13
申请号：US479549
申请日：1995-06-07
申请人： Michael Shaw , Gary W. Slater
发明人： Michael Shaw , Gary W. Slater
IPC分类号： A63B37/00 , A63B37/14
CPC分类号： A63B37/0004 , A63B37/0006 , A63B37/0018 , A63B37/0021 , A63B37/0053 , A63B37/0074 , A63B37/0075
摘要： A dimpled golf ball is provided with a regular repeating dimple pattern by projecting on to the surface of the ball the edges of a regular octahedron, thereby forming eight equilateral triangles on the surface. Each of the eight equilateral triangles can then be divided into sub-triangles so that there are four, six, seven or nine dimple-free great circles on the ball.
摘要翻译：凹凸高尔夫球通过在正面八面体的边缘上投射到球的表面上，从而在表面上形成八个等边三角形而设置有规则的重复凹坑图案。然后可以将八个等边三角形中的每一个分成三角形，使得球上有四个，六个，七个或九个无凹坑的大圆。

3. 发明授权

US07371533B2 Methods for separation of polymeric compounds 有权
标题翻译：聚合物分离方法
公开(公告)号：US07371533B2
公开(公告)日：2008-05-13
申请号：US11241990
申请日：2005-10-04
申请人： Gary W. Slater , Laurette C. McCormick , Annelise E Barron , Robert J. Meagher
发明人： Gary W. Slater , Laurette C. McCormick , Annelise E Barron , Robert J. Meagher
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C07K1/13 , C07K1/26 , C12Q1/6816 , C12Q2525/185 , C12Q2565/125
摘要： Recently two techniques using free solution electrophoresis to separate charged-uncharged polymer conjugates have proven successful: End Labeled Free Solution Electrophoresis (ELFSE) for DNA sequencing, and Free Solution Conjugate Electrophoresis (FSCE) for molar mass profiling of uncharged polymers. Previous attempts have been made to analyze experimental data generated by these new techniques for the electrophoresis of molecules with varying charge distributions. However, the importance of the ends of the polymers in determining the polymer's overall mobility was neglected in previous work. Through a careful investigation and a reanalysis of the experimental data, it is determined here that this “end effect” critically impacts the behavior of polymers and charged-uncharged polymer conjugates during electrophoresis. In this way, the invention provides for methods that exploit this “end effect” for the separation of polymeric molecules on the basis of size, including for example DNA separation and sequencing techniques.
摘要翻译：最近，使用自由溶液电泳分离带电荷的聚合物共轭物的两种技术已被证明是成功的：用于DNA测序的末端标记的游离溶液电泳（ELFSE）和用于不带电聚合物的摩尔质谱分析的游离溶液共轭电泳（FSCE）。以前曾经尝试分析由这些新技术产生的用于具有不同电荷分布的分子的电泳产生的实验数据。然而，聚合物末端在确定聚合物整体流动性方面的重要性在以前的工作中被忽略。通过对实验数据的仔细研究和重新分析，这里确定，这种“最终效应”严重影响聚合物和带电荷电聚合物共轭物在电泳过程中的行为。以这种方式，本发明提供了利用基于大小（包括例如DNA分离和测序技术）分离聚合物分子的这种“最终效应”的方法。

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