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1. 发明授权

US07371533B2 Methods for separation of polymeric compounds 有权
标题翻译：聚合物分离方法
公开(公告)号：US07371533B2
公开(公告)日：2008-05-13
申请号：US11241990
申请日：2005-10-04
申请人： Gary W. Slater , Laurette C. McCormick , Annelise E Barron , Robert J. Meagher
发明人： Gary W. Slater , Laurette C. McCormick , Annelise E Barron , Robert J. Meagher
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04
CPC分类号： C07K1/13 , C07K1/26 , C12Q1/6816 , C12Q2525/185 , C12Q2565/125
摘要： Recently two techniques using free solution electrophoresis to separate charged-uncharged polymer conjugates have proven successful: End Labeled Free Solution Electrophoresis (ELFSE) for DNA sequencing, and Free Solution Conjugate Electrophoresis (FSCE) for molar mass profiling of uncharged polymers. Previous attempts have been made to analyze experimental data generated by these new techniques for the electrophoresis of molecules with varying charge distributions. However, the importance of the ends of the polymers in determining the polymer's overall mobility was neglected in previous work. Through a careful investigation and a reanalysis of the experimental data, it is determined here that this “end effect” critically impacts the behavior of polymers and charged-uncharged polymer conjugates during electrophoresis. In this way, the invention provides for methods that exploit this “end effect” for the separation of polymeric molecules on the basis of size, including for example DNA separation and sequencing techniques.
摘要翻译：最近，使用自由溶液电泳分离带电荷的聚合物共轭物的两种技术已被证明是成功的：用于DNA测序的末端标记的游离溶液电泳（ELFSE）和用于不带电聚合物的摩尔质谱分析的游离溶液共轭电泳（FSCE）。以前曾经尝试分析由这些新技术产生的用于具有不同电荷分布的分子的电泳产生的实验数据。然而，聚合物末端在确定聚合物整体流动性方面的重要性在以前的工作中被忽略。通过对实验数据的仔细研究和重新分析，这里确定，这种“最终效应”严重影响聚合物和带电荷电聚合物共轭物在电泳过程中的行为。以这种方式，本发明提供了利用基于大小（包括例如DNA分离和测序技术）分离聚合物分子的这种“最终效应”的方法。

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