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1. 发明授权

US06777218B1 Subtilase enzymes having an improved wash performance on egg stains 失效
标题翻译：在蛋渍上具有改善的洗涤性能的副溶酶酶
公开(公告)号：US06777218B1
公开(公告)日：2004-08-17
申请号：US09533480
申请日：2000-03-23
申请人： Frank Mikkelsen , Tina Sejersgård Fanø , Jon E. Ness , Mark D. Welch , Lorraine J. Giver , Jeremy S. Minshull , Torben V. Borchert , Joel Cherry
发明人： Frank Mikkelsen , Tina Sejersgård Fanø , Jon E. Ness , Mark D. Welch , Lorraine J. Giver , Jeremy S. Minshull , Torben V. Borchert , Joel Cherry
IPC分类号： C12N952
CPC分类号： C12Y304/21062 , C12N9/50 , C12N9/54
摘要： The present invention relates to novel subtilases having an improved wash performance on egg stains. The present invention also relates to isolated nucleic acid sequences encoding the subtilases, nucleic acid constructs, recombinant expression vectors, host cells comprising the nucleic acid construct, and methods for producing and using the subtilases of the invention. Further, the present invention relates to cleaning and detergent compositions comprising the subtilase enzymes of the invention as well as to use of such enzymes in detergent compositions and for removal of egg stains.
摘要翻译：本发明涉及具有改善的蛋渍洗涤性能的新型脱水酶。本发明还涉及编码枯草芽孢杆菌，核酸构建体，重组表达载体，包含核酸构建体的宿主细胞的分离的核酸序列，以及用于产生和使用本发明的辅酶的方法。此外，本发明涉及包含本发明的脱羧酶的清洁和洗涤剂组合物，以及在洗涤剂组合物中使用这些酶并除去蛋渍。

2. 发明申请

US20100105599A1 SUBTILISIN VARIANTS 审中-公开
公开(公告)号：US20100105599A1
公开(公告)日：2010-04-29
申请号：US12562517
申请日：2009-09-18
申请人： Jon E. Ness , Mark Welch , Lorraine J. Giver , Joel R. Cherry , Torben V. Borchert , Jeremy S. Minshull
发明人： Jon E. Ness , Mark Welch , Lorraine J. Giver , Joel R. Cherry , Torben V. Borchert , Jeremy S. Minshull
IPC分类号： C12N9/56 , C11D7/42
CPC分类号： C12N9/6408 , C12N9/54
摘要： New subtilisin homologues (both nucleic acids and proteins) are provided. Compositions which include these new proteins, recombinant cells, shuffling methods involving the new homologues, antibodies to the new homologues, and methods of using the homologues are also provided.
摘要翻译：提供了新的枯草杆菌蛋白酶同源物（核酸和蛋白质）。还提供了包括这些新蛋白质，重组细胞，涉及新同系物的洗牌方法，新同系物的抗体以及使用同源物的方法的组合物。

3. 发明授权

US08014961B2 Integrated systems and methods for diversity generation and screening 有权
标题翻译：用于多样化生成和筛选的综合系统和方法
公开(公告)号：US08014961B2
公开(公告)日：2011-09-06
申请号：US11677505
申请日：2007-02-21
申请人： Steven H. Bass , Simon Christopher Davis , Phillip A. Patten , Matthew Tobin , Jeremy S. Minshull , Mark Welch , Claus Gustafsson , Brian Carr , Stephan J. Jenne , Sun Ai Raillard , Andreas Crameri , Willem P. C. Stemmer , Robin Emig , Pascal Longchamp , Stanley Goldman , Lorraine J. Giver , Joseph A. Affholter
发明人： Steven H. Bass , Simon Christopher Davis , Phillip A. Patten , Matthew Tobin , Jeremy S. Minshull , Mark Welch , Claus Gustafsson , Brian Carr , Stephan J. Jenne , Sun Ai Raillard , Andreas Crameri , Willem P. C. Stemmer , Robin Emig , Pascal Longchamp , Stanley Goldman , Lorraine J. Giver , Joseph A. Affholter
IPC分类号： G01N33/48
CPC分类号： C12N15/1031 , B01J19/0046 , B01J2219/00378 , B01J2219/00585 , B01J2219/00659 , B01J2219/00675 , B01J2219/00689 , B01J2219/00695 , B01J2219/007 , B01J2219/00722 , B82Y30/00 , C12N15/1027 , C40B60/04 , G06F19/18 , Y02A90/22
摘要： Integrated systems and methods for diversity generation and screening are provided. The systems use common fluid and array handling components to provide nucleic acid diversification, transcription, translation, product screening and subsequent diversification reactions.
摘要翻译：提供了用于多样化生成和筛选的集成系统和方法。该系统使用普通液体和阵列处理组分来提供核酸多样化，转录，翻译，产物筛选和随后的多样化反应。

4. 发明授权

US08825411B2 Design, synthesis and assembly of synthetic nucleic acids 有权
标题翻译：合成核酸的设计，合成和装配
公开(公告)号：US08825411B2
公开(公告)日：2014-09-02
申请号：US11579704
申请日：2005-05-04
申请人： Sridhar Govindarajan , Jeremy S. Minshull , Jon E. Ness
发明人： Sridhar Govindarajan , Jeremy S. Minshull , Jon E. Ness
IPC分类号： G06F19/00 , G06G7/48 , C40B30/00 , C12N15/10 , C12Q1/68 , G06F19/20
CPC分类号： C12N15/1027 , C12N15/1089 , C12Q1/6806 , G06F19/20
摘要： Methods of synthesizing oligonucleotides with high coupling efficiency (>99.5%) are provided. Methods for purification of synthetic oligonucleotides are also provided. Instrumentation configurations for oligonucleotide synthesis are also provided. Methods of designing and synthesizing polynucleotides are also provided. Polynucleotide design is optimized for subsequent assembly from shorter oligonucleotides. Modifications of phosphoramidite chemistry to improve the subsequent assembly of polynucleotides are provided. The design process also incorporates codon biases into polynucleotides that favor expression in defined hosts. Design and assembly methods are also provided for the efficient synthesis of sets of polynucleotide variants. Software to automate the design and assembly process is also provided.
摘要翻译：提供了具有高耦合效率的寡核苷酸（> 99.5％）的方法。还提供了纯化合成寡核苷酸的方法。也提供寡核苷酸合成的仪器配置。还提供了设计和合成多核苷酸的方法。多核苷酸设计被优化用于从较短的寡核苷酸的后续装配。提供亚磷酰胺化学改性以改进随后的多核苷酸组装。设计过程还将密码子偏差结合到有利于在定义的宿主中表达的多核苷酸。还提供了设计和组装方法以有效合成多组DNA变体组。还提供了自动化设计和组装过程的软件。

5. 发明授权

US08323930B2 Methods, compositions and kits for one-step DNA cloning using DNA topoisomerase 有权
标题翻译：使用DNA拓扑异构酶进行一步DNA克隆的方法，组合物和试剂盒
公开(公告)号：US08323930B2
公开(公告)日：2012-12-04
申请号：US12178506
申请日：2008-07-23
申请人： Jon E. Ness , Jeremy S. Minshull
发明人： Jon E. Ness , Jeremy S. Minshull
IPC分类号： C12P19/34 , C12Q1/68
CPC分类号： C12N15/66 , C12N9/90 , C12N15/10 , C12N15/64
摘要： Provided are methods, compositions, and kits for molecular cloning of DNA using DNA topoisomerase. The methods comprise (I) combining into a mixture (A) a first polynucleotide, comprising an origin of replication, a selectable marker, two topoisomerase recognition sequences, and two nicking agent recognition sequences, each of the topoisomerase recognition sequences being within 50 nucleotides of at least one of the nicking agent recognition sequences and each of two nicking agent recognition sequences being nicked, with (B) a sequence-specific topoisomerase and (C) a second polynucleotide, having a 5′ hydroxyl on each end; and (II) transforming the mixture into a host organism, thereby cloning the second polynucleotide. Formation or purification of a DNA-protein adduct prior to the addition of the second polynucleotide is not required. Also provided are vector sequences to facilitate performance of the methods and methods for modifying a vector of interest to render it useful in the disclosed methods.
摘要翻译：提供了使用DNA拓扑异构酶分子克隆DNA的方法，组合物和试剂盒。方法包括（I）将混合物（A）组合成包含复制起点的第一多核苷酸，选择性标记，两个拓扑异构酶识别序列和两个切口识别序列，每个拓扑异构酶识别序列在50个核苷酸内具有（B）序列特异性拓扑异构酶的切割剂识别序列和两个切口剂识别序列中的至少一个，（C）第二多核苷酸，每个末端具有5'羟基; 和（II）将混合物转化为宿主生物体，从而克隆第二多核苷酸。不需要在加入第二个多核苷酸之前形成或纯化DNA-蛋白质加合物。还提供了用于促进用于修饰感兴趣的载体以使其在所公开的方法中有用的方法和方法的性能的载体序列。

6. 发明申请

US20090317873A1 Design, synthesis and assembly of synthetic nucleic acids 审中-公开
标题翻译：合成核酸的设计，合成和装配
公开(公告)号：US20090317873A1
公开(公告)日：2009-12-24
申请号：US11579568
申请日：2005-05-04
申请人： Sridhar Govindarajan , Nicolay V. Kulikov , Jeremy S. Minshull , Jon E. Ness
发明人： Sridhar Govindarajan , Nicolay V. Kulikov , Jeremy S. Minshull , Jon E. Ness
IPC分类号： C12P19/34 , C12M1/00
CPC分类号： C07H21/00
摘要： Methods of synthesizing oligonucleotides with high coupling efficiency (>99.5%) are provided. Methods for purification of synthetic oligonucleotides are also provided. Instrumentation configurations for oligonucleotide synthesis are also provided. Methods of designing and synthesizing polynucleotides are also provided. Polynucleotide design is optimized for subsequent assembly from shorter oligonucleotides. Modifications of phosphoramidite chemistry to improve the subsequent assembly of polynucleotides are provided. The design process also incorporates codon biases into polynucleotides that favor expression in defined hosts. Design and assembly methods are also provided for the efficient synthesis of sets of polynucleotide variants. Software to automate the design and assembly process is also provided.
摘要翻译：提供了具有高耦合效率的寡核苷酸（> 99.5％）的方法。还提供了纯化合成寡核苷酸的方法。也提供寡核苷酸合成的仪器配置。还提供了设计和合成多核苷酸的方法。多核苷酸设计被优化用于从较短的寡核苷酸的后续装配。提供亚磷酰胺化学改性以改进随后的多核苷酸组装。设计过程还将密码子偏差结合到有利于在定义的宿主中表达的多核苷酸。还提供了设计和组装方法以有效合成多组DNA变体组。还提供了自动化设计和组装过程的软件。

7. 发明申请

US20130196864A1 DESIGN, SYNTHESIS AND ASSEMBLY OF SYNTHETIC NUCLEIC ACIDS 审中-公开
标题翻译：合成核酸的设计，合成和组装
公开(公告)号：US20130196864A1
公开(公告)日：2013-08-01
申请号：US13763529
申请日：2013-02-08
申请人： Sridhar Govindarajan , Jeremy S. Minshull , Jon E. Ness
发明人： Sridhar Govindarajan , Jeremy S. Minshull , Jon E. Ness
IPC分类号： C12N15/10
CPC分类号： C12N15/1027 , C12N15/1089 , C12Q1/6806 , G16B25/00
摘要： Methods of synthesizing oligonucleotides with high coupling efficiency (>99.5%) are provided. Methods for purification of synthetic oligonucleotides are also provided. Instrumentation configurations for oligonucleotide synthesis are also provided. Methods of designing and synthesizing polynucleotides are also provided. Polynucleotide design is optimized for subsequent assembly from shorter oligonucleotides. Modifications of phosphoramidite chemistry to improve the subsequent assembly of polynucleotides are provided. The design process also incorporates codon biases into polynucleotides that favor expression in defined hosts. Design and assembly methods are also provided for the efficient synthesis of sets of polynucleotide variants. Software to automate the design and assembly process is also provided.
摘要翻译：提供了具有高耦合效率的寡核苷酸（> 99.5％）的方法。还提供了纯化合成寡核苷酸的方法。也提供寡核苷酸合成的仪器配置。还提供了设计和合成多核苷酸的方法。多核苷酸设计被优化用于从较短的寡核苷酸的后续装配。提供亚磷酰胺化学改性以改进随后的多核苷酸组装。设计过程还将密码子偏差结合到有利于在定义的宿主中表达的多核苷酸。还提供了设计和组装方法以有效合成多组DNA变体组。还提供了自动化设计和组装过程的软件。

8. 发明授权

US08377681B2 Evolution of whole cells and organisms by recursive sequence recombination 有权
公开(公告)号：US08377681B2
公开(公告)日：2013-02-19
申请号：US13310332
申请日：2011-12-02
申请人： Stephen DelCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen DelCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
IPC分类号： C12N1/16 , C12N1/18
CPC分类号： C12N15/102 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12P17/162 , C12Q1/68 , C12Q1/6811 , C12Q1/6827 , C12R1/465
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

9. 发明授权

US07873477B1 Method and system using systematically varied data libraries 有权
标题翻译：使用系统化的数据库的方法和系统
公开(公告)号：US07873477B1
公开(公告)日：2011-01-18
申请号：US10225564
申请日：2002-08-20
申请人： Claes Gustafsson , Sridhar Govindarajan , Jeremy S. Minshull , Jon E. Ness , Robin A. Emig
发明人： Claes Gustafsson , Sridhar Govindarajan , Jeremy S. Minshull , Jon E. Ness , Robin A. Emig
IPC分类号： G06F19/00 , C40B50/00 , C40B50/02
CPC分类号： C40B50/02 , G06F19/14 , G06F19/18 , G06F19/28
摘要： Methods and systems for providing biological results in the form of systematically varied libraries of sequences or as data representing sequences or physical preparations of systematically varied libraries and/or selections from systematically varied libraries.
摘要翻译：用于以系统变化的序列库的形式提供生物结果的方法和系统，或作为代表系统变化的文库的序列或物理制备物的数据和/或来自系统变化的文库的选择的方法和系统。

10. 发明授权

US07629170B2 Evolution of whole cells and organisms by recursive sequence recombination 有权
公开(公告)号：US07629170B2
公开(公告)日：2009-12-08
申请号：US11506215
申请日：2006-08-17
申请人： Stephen delCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
发明人： Stephen delCardayre , Matthew B. Tobin , Willem P. C. Stemmer , Jon E. Ness , Jeremy S. Minshull , Phillip A. Patten , Venkiteswatan Mani Subramanian , Linda A. Castle , Claus M. Krebber , Steven H. Bass , Ying-Xin Zhang , Anthony R. Cox , Gjalt W. Huisman , Ling Yuan , Joseph A. Affholter
IPC分类号： C12N15/00 , C12N15/87 , C12N15/63
CPC分类号： C12R1/465 , A01K2267/02 , C07K14/245 , C12N15/1027 , C12N15/8271 , C12N15/8273 , C12N15/8279 , C12N15/90 , C12P17/162 , C12Q1/6811 , C12Q1/6827
摘要： The invention provides methods employing iterative cycles of recombination and selection/screening for evolution of whole cells and organisms toward acquisition of desired properties. Examples of such properties include enhanced recombinogenicity, genome copy number, and capacity for expression and/or secretion of proteins and secondary metabolites.

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