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    • 6. 发明授权
    • Method of detecting and counting microorganisms
    • 微生物检测和计数方法
    • US5789191A
    • 1998-08-04
    • US602748
    • 1996-02-21
    • Bianca MayerGerhard SauermannBernd TraupeFlorian Wolf
    • Bianca MayerGerhard SauermannBernd TraupeFlorian Wolf
    • C12N1/38C12Q1/04C12Q1/06C12Q1/02C12Q1/18
    • C12N1/38C12Q1/04C12Q2304/46Y10S435/848Y10S435/849Y10S435/882Y10S435/883Y10S435/884Y10S435/921Y10S435/922Y10S435/923
    • The invention provides a cosmetic or dermatological method for detecting and/or selectively quantifying individual microorganisms, and/or whole groups of microorganisms, which are present on human or animal skin, comprising the steps of removing a sample of the microflora of the human or animal skin, treating the sample with a deinhibiting medium, adding the treated sample to a culture medium which exhibits favorable growth conditions for a defined group of microorganisms but unfavorable growth conditions for other microorganisms, to produce a selective culture, and incubating the selective culture over a sufficiently long period of time, to allow only the group of microorganisms for which the culture medium exhibits favorable growth conditions the opportunity to multiply, in association with metabolic products, in particular CO.sub.2, produced which collect either in the culture medium itself or in a test vessel which is provided for the purpose and which contains an indicator medium, and the concentration of the metabolic products is elucidated by means of the change in the alternating current (AC) resistance of the culture medium and/or the indicator medium in the test vessel and, after appropriate calibration, is correlated by means of arithmetical methods with the number of microorganisms in the selective medium.
    • PCT No.PCT / DE94 / 00953 Sec。 371日期1996年2月21日 102(e)日期1996年2月21日PCT 1994年8月17日PCT公布。 公开号WO95 / 06133 日期1995年3月2日本发明提供了用于检测和/或选择性定量存在于人或动物皮肤上的个体微生物和/或整组微生物的化妆品或皮肤病学方法,包括以下步骤:除去微生物群落样品 的人或动物皮肤,用去绝菌培养基处理样品,将经处理的样品加入培养基中,所述培养基对于所定义的微生物组,对其它微生物表现出良好的生长条件,但不利于其它微生物的生长条件,以产生选择性培养物,并孵育 在足够长的时间段内的选择性培养,仅允许培养基显示有利生长条件的一组微生物与在培养基中收集的代谢产物特别是二氧化碳相结合的机会倍增 本身或在为该目的而提供并且包含标记的试验容器中 通过培养基和/或测试容器中的指示剂介质的交流(AC)电阻的变化来阐明代谢产物的浓度,并且在适当的校准之后,通过 具有选择性培养基中微生物数量的算术方法。
    • 9. 发明授权
    • Cosmetic and skin care sticks with high water contents
    • 化妆品和护肤品含有高含水量
    • US06613338B1
    • 2003-09-02
    • US09284369
    • 1999-04-14
    • Jörg SchreiberKhiet Hien DiecFlorian WolfManfred Klier
    • Jörg SchreiberKhiet Hien DiecFlorian WolfManfred Klier
    • A61K600
    • A61Q19/00A61K8/02A61K8/0229A61K8/39A61K8/86A61Q1/06A61Q1/10A61Q17/04Y10S514/938Y10S514/941
    • Lipsticks, antiacne sticks, sunscreen sticks and eyeshadow sticks, with a high water content, characterized in that they comprise (a) a lipid phase, which comprises (a1) at least one oil component (a2) at least one wax component (a3) optionally other substances soluble or dispersible in the lipid phase, (b) an aqueous phase, which comprises (b1) from 30 to 85% by weight of water, based on the total weight of the stick composition and (b2) if desired, substances soluble or dispersible in water, (c) at least one active ingredient or several chosen from the active ingredients known for lipsticks, antiacne sticks, sunscreen sticks and eyeshadow sticks, (d) a W/O emulsifier or a mixture of two or more W/O emulsifiers, (e) one or more stabilizers, chosen from the group of substances of the general structure A—B—A′, where A and A′ are identical or different hydrophobic organic radicals, and B is a hydrophilic group, (f) if desired, further surface-active substances as coemulsifiers, and also, if desired, stabilizers and further customary cosmetic and/or pharmaceutical auxiliaries, active ingredients and/or additives.
    • 具有高水分含量的唇膏,抗痤疮棒,防晒霜和眼影棒,其特征在于它们包含(a)脂质相,其包含(a1)至少一种油组分(a2)至少一种蜡组分(a3) 任选的其它可溶于或可分散在脂质相中的物质,(b)水相,其包含(b1)30至85重量%的水,基于棒组合物的总重量,和(b2)如果需要,物质 (c)至少一种活性成分或几种,其选自已知用于唇膏,抗痤疮棒,防晒霜和眼影棒的活性成分,(d)W / O乳化剂或两种或更多种W的混合物 / O乳化剂,(e)一种或多种选自一般结构AB-A'的物质的稳定剂,其中A和A'是相同或不同的疏水性有机基团,B是亲水基团,(f) 如果需要,可以使用作为辅助乳化剂的更多的表面活性物质,以及如果需要的话 ,稳定剂和进一步的常规化妆品和/或药物助剂,活性成分和/或添加剂。