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1. 发明授权

US5789191A Method of detecting and counting microorganisms 失效
标题翻译：微生物检测和计数方法
公开(公告)号：US5789191A
公开(公告)日：1998-08-04
申请号：US602748
申请日：1996-02-21
申请人： Bianca Mayer , Gerhard Sauermann , Bernd Traupe , Florian Wolf
发明人： Bianca Mayer , Gerhard Sauermann , Bernd Traupe , Florian Wolf
IPC分类号： C12N1/38 , C12Q1/04 , C12Q1/06 , C12Q1/02 , C12Q1/18
CPC分类号： C12N1/38 , C12Q1/04 , C12Q2304/46 , Y10S435/848 , Y10S435/849 , Y10S435/882 , Y10S435/883 , Y10S435/884 , Y10S435/921 , Y10S435/922 , Y10S435/923
摘要： The invention provides a cosmetic or dermatological method for detecting and/or selectively quantifying individual microorganisms, and/or whole groups of microorganisms, which are present on human or animal skin, comprising the steps of removing a sample of the microflora of the human or animal skin, treating the sample with a deinhibiting medium, adding the treated sample to a culture medium which exhibits favorable growth conditions for a defined group of microorganisms but unfavorable growth conditions for other microorganisms, to produce a selective culture, and incubating the selective culture over a sufficiently long period of time, to allow only the group of microorganisms for which the culture medium exhibits favorable growth conditions the opportunity to multiply, in association with metabolic products, in particular CO.sub.2, produced which collect either in the culture medium itself or in a test vessel which is provided for the purpose and which contains an indicator medium, and the concentration of the metabolic products is elucidated by means of the change in the alternating current (AC) resistance of the culture medium and/or the indicator medium in the test vessel and, after appropriate calibration, is correlated by means of arithmetical methods with the number of microorganisms in the selective medium.
摘要翻译： PCT No.PCT / DE94 / 00953 Sec。 371日期1996年2月21日 102（e）日期1996年2月21日PCT 1994年8月17日PCT公布。公开号WO95 / 06133 日期1995年3月2日本发明提供了用于检测和/或选择性定量存在于人或动物皮肤上的个体微生物和/或整组微生物的化妆品或皮肤病学方法，包括以下步骤：除去微生物群落样品的人或动物皮肤，用去绝菌培养基处理样品，将经处理的样品加入培养基中，所述培养基对于所定义的微生物组，对其它微生物表现出良好的生长条件，但不利于其它微生物的生长条件，以产生选择性培养物，并孵育在足够长的时间段内的选择性培养，仅允许培养基显示有利生长条件的一组微生物与在培养基中收集的代谢产物特别是二氧化碳相结合的机会倍增本身或在为该目的而提供并且包含标记的试验容器中通过培养基和/或测试容器中的指示剂介质的交流（AC）电阻的变化来阐明代谢产物的浓度，并且在适当的校准之后，通过具有选择性培养基中微生物数量的算术方法。

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