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    • 1. 发明申请
    • Imaging
    • 成像
    • US20060124870A1
    • 2006-06-15
    • US10531007
    • 2003-10-15
    • Fedja BobanovicJohn PhillipsShab LadhaPatrick Courtney
    • Fedja BobanovicJohn PhillipsShab LadhaPatrick Courtney
    • G21G4/00
    • G02B21/0044G02B21/0064G02B21/0076G02B21/008
    • A method of imaging light from a specimen in which excitation light passes to the specimen via a scanning system and light emitted by luminescence of the specimen passes in another direction via the scanning system to an image capture device having a sensor having discrete spatially distinct light sensitive regions. The scanning system is operated so as to scan the whole of an area of interest of the specimen, and the excitation light and/or the image capture device are controlled so that light emitted from the specimen is only incident on the image capture device sensor for a specific time period equal to that required for scanning the whole of the area of interest n times (where n is a whole number equal to or greater than 1). The scanning system is a confocal system, and in one example comprises a rotating Nipkow disc scanner and the pattern of openings in the disc is such that rotation of the disc through A° results in scanning the whole of the area of interest and the said specific time period is selected to correspond to nA° of disc rotation. Apparatus for performing the method to produce a video signal for creating an image in a display device or for processing and analysis by a computer, includes control means adapted to control the excitation light and/or the image capture device so that light from the specimen is incident on the image capture device for the specific period of time, and the image capture device is a CCD camera.
    • 一种对来自试样的光进行成像的方法,其中激发光通过扫描系统传递到样本,并且通过扫描系统的发光发射的光通过扫描系统的另一方向到具有离散空间上不同光敏感的传感器的图像捕获装置 地区。 操作扫描系统以扫描样本的整个感兴趣区域,并且控制激发光和/或图像捕获装置,使得从样本发射的光仅入射到图像捕获装置传感器上 与扫描整个感兴趣区域n次(其中n是等于或大于1的整数)所需的特定时间段相等。 扫描系统是共焦系统,并且在一个示例中包括旋转的Nipkow盘扫描器,并且盘中的开口图案使得盘通过A°的旋转导致扫描感兴趣的整个区域,并且所述特定 选择时间段以对应于盘旋转的nA°。 用于执行产生用于在显示装置中创建图像或由计算机进行处理和分析的视频信号的方法的装置包括适于控制激发光和/或图像捕获装置的控制装置,使得来自标本的光为 在特定时间段内对图像捕获装置发生事件,并且图像捕获装置是CCD照相机。
    • 2. 发明授权
    • Imaging
    • 成像
    • US08289620B2
    • 2012-10-16
    • US10531007
    • 2003-10-15
    • Fedja BobanovicJohn PhillipsShab LadhaPatrick Courtney
    • Fedja BobanovicJohn PhillipsShab LadhaPatrick Courtney
    • G02B21/00
    • G02B21/0044G02B21/0064G02B21/0076G02B21/008
    • A method of imaging light from a specimen in which excitation light passes to the specimen via a scanning system and light emitted by luminescence of the specimen passes in another direction via the scanning system to an image capture device having a sensor having discrete spatially distinct light sensitive regions. The scanning system is operated to scan the whole of an area of interest of the specimen, and the excitation light and/or the image capture device are controlled so that light emitted from the specimen in only incident on the image capture device sensor for a specific time period equal to that required for scanning the whole of the area of interest n time. The scanning system is a confocal system. Apparatus is provided for performing the method to produce a video signal for creating an image in a display device or for processing and analysis by a computer.
    • 一种对来自试样的光进行成像的方法,其中激发光通过扫描系统传递到样本,并且通过扫描系统的发光发射的光通过扫描系统的另一方向到具有离散空间上不同光敏感的传感器的图像捕获装置 地区。 操作扫描系统以扫描样本的整个感兴趣区域,并且控制激发光和/或图像捕获装置,使得从样本发射的光仅仅入射到图像捕获装置传感器上用于特定的 时间段等于扫描整个感兴趣区域所需的时间。 扫描系统是共焦系统。 提供了用于执行产生用于在显示装置中创建图像或由计算机进行处理和分析的视频信号的方法的装置。