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    • 4. 发明授权
    • Highly alkaline proteases
    • 高碱性蛋白酶
    • US5352603A
    • 1994-10-04
    • US661378
    • 1991-02-28
    • Roman VetterDetlef WilkeAntoine AmoryAndre ClippeDietmar SchomburgWolfgang Aehle
    • Roman VetterDetlef WilkeAntoine AmoryAndre ClippeDietmar SchomburgWolfgang Aehle
    • C12N1/21C12N9/54C12N15/57C11D3/386C11D7/42C12N9/52
    • C12N9/54
    • Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.
    • 通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。 突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得,通过定点诱变(点突变)以定点诱变(点突变)改变这些DNA序列,使得点突变位于的密码子现在 编码比原始氨基酸更强碱性的氨基酸。 结果是高碱性蛋白酶,其中原始氨基酸已被更强碱性氨基酸替代,优选由氨基酸赖氨酸或精氨酸代替。 还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸,DNA序列,载体和转化微生物。