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1. 发明授权

US5352603A Highly alkaline proteases 失效
标题翻译：高碱性蛋白酶
公开(公告)号：US5352603A
公开(公告)日：1994-10-04
申请号：US661378
申请日：1991-02-28
申请人： Roman Vetter , Detlef Wilke , Antoine Amory , Andre Clippe , Dietmar Schomburg , Wolfgang Aehle
发明人： Roman Vetter , Detlef Wilke , Antoine Amory , Andre Clippe , Dietmar Schomburg , Wolfgang Aehle
IPC分类号： C12N1/21 , C12N9/54 , C12N15/57 , C11D3/386 , C11D7/42 , C12N9/52
CPC分类号： C12N9/54
摘要： Novel, optimized highly alkaline proteases which are suitable for use in detergent formulations are prepared by employing microorganisms transformed by mutated DNA sequences. The mutated sequences are obtained starting from DNA sequences which code for highly alkaline protease usually produced by Bacillus species by altering these DNA sequences in defined positions by directed mutagenesis (point mutation) in such a way that the codon in which the point mutation is located now codes for an amino acid which is more strongly basic than the original amino acid. The result is highly alkaline proteases in which original amino acids have been replaced by more strongly basic amino acids, preferably by the amino acids lysine or arginine. Synthetic oligonucleotides, DNA sequences, vectors and transformed microorganisms which are used for generating and obtaining the optimized highly alkaline protease are also described.
摘要翻译：通过使用通过突变的DNA序列转化的微生物来制备适用于洗涤剂制剂的新型优化的高碱性蛋白酶。突变序列从编码通过芽孢杆菌属物种生产的高度碱性蛋白酶的DNA序列开始获得，通过定点诱变（点突变）以定点诱变（点突变）改变这些DNA序列，使得点突变位于的密码子现在编码比原始氨基酸更强碱性的氨基酸。结果是高碱性蛋白酶，其中原始氨基酸已被更强碱性氨基酸替代，优选由氨基酸赖氨酸或精氨酸代替。还描述了用于产生和获得优化的高碱性蛋白酶的合成寡核苷酸，DNA序列，载体和转化微生物。

2. 发明授权

US5453372A Stabilized enzymes and process for preparing them 失效
标题翻译：稳定的酶及其制备方法
公开(公告)号：US5453372A
公开(公告)日：1995-09-26
申请号：US918318
申请日：1992-07-27
申请人： Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
发明人： Roman Vetter , Ingo Muecke , Detlef Wilke , Amory Antoine , Wolfgang Aehle , Harald Sobek , Dietmar Schomburg , Andre Clippe
IPC分类号： C11D3/386 , C12N9/00 , C12N9/20 , C12N9/54 , C12N15/57 , C12N9/50 , C12N9/56
CPC分类号： C11D3/38663 , C11D3/386 , C12N9/00 , C12N9/20 , C12N9/54
摘要： Enzymes stabilized against the destabilizing effect of ionic surfactants and a method of producing such stabilized enzymes in which DNA sequences which code for the enzymes are modified by directed mutation in defined positions which correlate with defined surface regions of the enzyme, in such a way that the codon in which the mutation is located now codes for an amino acid differing from the original amino acid.
摘要翻译：针对离子表面活性剂的不稳定作用而进行的酶稳定化，以及生产这样的稳定化酶的方法，其中编码酶的DNA序列通过与定义的酶的表面区域相关的限定位置的定向突变来修饰，突变位于的密码子现在编码与原始氨基酸不同的氨基酸。

3. 发明授权

US09469848B2 Variants of glucoamylase 有权
标题翻译：葡糖淀粉酶的变体
公开(公告)号：US09469848B2
公开(公告)日：2016-10-18
申请号：US13806398
申请日：2011-06-30
申请人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
发明人： Peter Edvard Degn , Richard R. Bott , Casper Willem Vroemen , Martijn Silvan Scheffers , Wolfgang Aehle , Elin Petersen
IPC分类号： C12P19/20 , C12N9/34 , C07H21/04 , C07K1/00 , C12N9/26 , A23L1/09 , C12C5/00 , C12C7/04 , C12P19/02 , C13K1/06
CPC分类号： C12N9/2408 , A23L29/35 , C12C5/004 , C12C7/04 , C12N9/2428 , C12P19/02 , C12P19/20 , C12Y302/01003 , C13K1/06 , Y02P20/52
摘要： The present invention relates to combinatorial variants of a parent glucoamylase that have altered properties for reducing the synthesis of condensation products during hydrolysis of starch. Accordingly, the variants of a parent glucoamylase are suitable such as for use within brewing and glucose syrup production. Also disclosed are DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译：本发明涉及母体葡糖淀粉酶的组合变体，其在淀粉水解期间具有降低缩合产物合成的性质。因此，母体葡糖淀粉酶的变体是适合的，例如用于酿造和葡萄糖浆生产中。还公开了编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。

4. 发明申请

US20120149087A1 GLUCOAMYLASE VARIANTS WITH ALTERED PROPERTIES 有权
标题翻译：具有更改属性的葡萄糖酶变体
公开(公告)号：US20120149087A1
公开(公告)日：2012-06-14
申请号：US13251116
申请日：2011-09-30
申请人： Wolfgang Aehle , Richard R. Bott , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
发明人： Wolfgang Aehle , Richard R. Bott , Martijn Scheffers , Piet Van Solingen , Casper Vroemen
IPC分类号： C12N9/34 , C12N15/63 , C12N1/19 , C12N1/15 , C12N5/10 , C12N15/56 , C12N1/21
CPC分类号： C12N9/2428 , C12P7/06 , C12P19/02 , C12P19/14 , Y02E50/17
摘要： The present disclosure relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present disclosure provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions and cleaning compositions. The disclosure also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译：本公开涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。特别地，本公开提供了包含变体葡糖淀粉酶的组合物，包括淀粉水解组合物和清洁组合物。本公开还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。

5. 发明申请

US20100267114A1 Glucoamylase Variants with Altered Properties 有权
标题翻译：具有改变特性的葡糖淀粉酶变体
公开(公告)号：US20100267114A1
公开(公告)日：2010-10-21
申请号：US12443255
申请日：2007-10-09
申请人： Wolfgang Aehle , Piet Van Solingen , Martin S. Scheffers , Richard R. Bott
发明人： Wolfgang Aehle , Piet Van Solingen , Martin S. Scheffers , Richard R. Bott
IPC分类号： C12N9/24 , C07H21/04 , C12N1/21 , C12N1/15 , C12N1/19 , C12N5/10
CPC分类号： C12N9/2428 , C12N15/09 , C12Y302/01003 , Y02E50/17
摘要： The present invention relates to variants of a parent glucoamylase having altered properties (e.g., improved thermostability and/or specific activity). In particular, the present invention provides compositions comprising the variant glucoamylases, including starch hydrolyzing compositions, animal feed compositions and cleaning compositions. The invention also relates to DNA constructs encoding the variants and methods of producing the glucoamylase variants in host cells.
摘要翻译：本发明涉及具有改变的性质（例如改善的热稳定性和/或比活性）的母体葡糖淀粉酶的变体。特别地，本发明提供包含变体葡糖淀粉酶，包括淀粉水解组合物，动物饲料组合物和清洁组合物的组合物。本发明还涉及编码在宿主细胞中产生葡糖淀粉酶变体的变体和方法的DNA构建体。

6. 发明申请

US20080063774A1 Multiple mutation variants of serine protease 有权
标题翻译：丝氨酸蛋白酶的多重突变变体
公开(公告)号：US20080063774A1
公开(公告)日：2008-03-13
申请号：US11809104
申请日：2007-05-31
申请人： Wolfgang Aehle , David Estell , Ronaldus Hommes , Brian Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran Poulose , Andrew Shaw , Wilhelmus Van Der Kley , Leonardus Van Marrewijk
发明人： Wolfgang Aehle , David Estell , Ronaldus Hommes , Brian Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran Poulose , Andrew Shaw , Wilhelmus Van Der Kley , Leonardus Van Marrewijk
IPC分类号： A23K1/00 , C07H21/00 , C11D7/42 , C12N1/00 , C12N1/15 , C12N1/21 , C12N15/63 , C12N9/50 , D06M16/00
CPC分类号： C12N9/52 , A23K20/189 , A23K50/75 , C11D3/386 , C12N9/96 , C12Y304/21 , D06M16/003
摘要： The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
摘要翻译：本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。特别地，本发明提供了具有多个取代的丝氨酸蛋白酶，编码这些蛋白酶的DNA，包含编码蛋白酶的DNA的载体，用载体DNA转化的宿主细胞和由宿主细胞产生的酶。本发明还提供了清洁组合物（例如洗涤剂组合物），动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。在特别优选的实施方案中，本发明提供衍生自本文所述的野生型蛋白酶的突变体（即，变体）蛋白酶。这些变体蛋白酶也可用于许多应用。

7. 发明授权

US6072015A Lignin based paint 有权
标题翻译：木质素基油漆
公开(公告)号：US6072015A
公开(公告)日：2000-06-06
申请号：US207302
申请日：1998-12-08
申请人： Rob Bolle , Wolfgang Aehle
发明人： Rob Bolle , Wolfgang Aehle
IPC分类号： C09D197/00 , C08H5/02 , C08K5/00
CPC分类号： C09D197/005
摘要： A method is provided for producing a painted article comprising: (a) preparing a lignin solution comprising lignin and a dye or pigment; (b) mixing the lignin solution with a phenol oxidizing enzyme; (c) incubating the mixture from said step (b) under conditions and for a time sufficient to form a solution of a desired viscosity; (d) contacting or spreading the mixture from the step (c) on an article to be painted; and (e) allowing the mixture to set onto the article by subjecting the article to conditions and for a time sufficient to form a paint on the surface of the article.
摘要翻译：提供了一种用于生产涂漆制品的方法，包括：（a）制备包含木质素和染料或颜料的木质素溶液; （b）将木质素溶液与酚氧化酶混合; （c）将所述步骤（b）的混合物在足以形成所需粘度的溶液的条件下培养一段时间; （d）将来自步骤（c）的混合物接触或铺展在待涂漆的物品上; 和（e）通过使制品经受物品表面上足以形成油漆的条件和时间使混合物置于物品上。

8. 发明授权

US08679816B2 Cellulase variants with improved expression, activity and stability, and use thereof 有权
标题翻译：具有改善的表达，活性和稳定性的纤维素酶变体及其用途
公开(公告)号：US08679816B2
公开(公告)日：2014-03-25
申请号：US13321928
申请日：2010-06-03
申请人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W. J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
发明人： Wolfgang Aehle , Richard R. Bott , Benjamin Bower , Jonathan Caspi , David A. Estell , Frits Goedegebuur , Ronaldus W. J. Hommes , Thijs Kaper , Bradley Kelemen , Slavko Kralj , Johan Van Lieshout , Igor Nikolaev , Sander Van Stigt Thans , Louise Wallace , Gudrun Vogtentanz , Mats Sandgren
IPC分类号： C12N9/42 , C12P19/00 , C12P19/02 , C07H21/04
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/17
摘要： The present disclosure relates to cellulase variants. In particular the present disclosure relates to cellulase variants having improved expression, activity and/or stability. Also described are nucleic acids encoding the cellulase variants, compositions comprising the cellulase variants, and methods of use thereof.
摘要翻译：本公开涉及纤维素酶变体。特别地，本公开涉及具有改善的表达，活性和/或稳定性的纤维素酶变体。还描述了编码纤维素酶变体的核酸，包含纤维素酶变体的组合物及其使用方法。

9. 发明授权

US08455234B2 Multiple mutation variants of serine protease 失效
标题翻译：丝氨酸蛋白酶的多重突变变体
公开(公告)号：US08455234B2
公开(公告)日：2013-06-04
申请号：US13162104
申请日：2011-06-16
申请人： Wolfgang Aehle , David A. Estell , Ronaldus W. J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A. H. van der Kley , Leonardus P. M. van Marrewijk
发明人： Wolfgang Aehle , David A. Estell , Ronaldus W. J. Hommes , Brian E. Jones , Marc Kolkman , Chris Leeflang , Hiroshi Oh , Ayrookaran J. Poulose , Andrew Shaw , Wilhelmus A. H. van der Kley , Leonardus P. M. van Marrewijk
IPC分类号： C12N9/48
CPC分类号： C12N9/52 , A23K20/189 , A23K50/75 , C11D3/386 , C12N9/96 , C12Y304/21 , D06M16/003
摘要： The present invention provides novel Micrococcineae spp serine proteases having multiple substitutions. In particular, the present invention provides serine proteases having multiple substitutions, DNA encoding these proteases, vectors comprising the DNA encoding the proteases, host cells transformed with the vector DNA, and enzymes produced by the host cells. The present invention also provides cleaning compositions (e.g., detergent compositions), animal feed compositions, and textile and leather processing compositions comprising these serine protease variants. In particularly preferred embodiments, the present invention provides mutant (i.e., variant) proteases derived from the wild-type proteases described herein. These variant proteases also find use in numerous applications.
摘要翻译：本发明提供了具有多个取代的新型微球菌spp丝氨酸蛋白酶。特别地，本发明提供了具有多个取代的丝氨酸蛋白酶，编码这些蛋白酶的DNA，包含编码蛋白酶的DNA的载体，用载体DNA转化的宿主细胞和由宿主细胞产生的酶。本发明还提供了清洁组合物（例如洗涤剂组合物），动物饲料组合物以及包含这些丝氨酸蛋白酶变体的纺织和皮革加工组合物。在特别优选的实施方案中，本发明提供衍生自本文所述的野生型蛋白酶的突变体（即，变体）蛋白酶。这些变体蛋白酶也可用于许多应用。

10. 发明申请

US20120164695A1 COMBINATORIAL VARIANTS OF GLUCOAMYLASE WITH IMPROVED SPECIFIC ACTIVITY AND/OR THERMOSTABILITY 审中-公开
标题翻译：具有改善的特异活性和/或热稳定性的葡萄糖酸酶的组合变体
公开(公告)号：US20120164695A1
公开(公告)日：2012-06-28
申请号：US13390381
申请日：2010-08-18
申请人： Wolfgang Aehle , Richard R. Bott , Martijn Silvan Scheffers , Casper Vroeman
发明人： Wolfgang Aehle , Richard R. Bott , Martijn Silvan Scheffers , Casper Vroeman
IPC分类号： C12N9/34 , C12N15/63 , C12P19/14 , C12N5/10 , C12N1/15 , C12N1/21 , C12N1/19 , C12N15/56 , C12P7/02
CPC分类号： C12P19/14 , A23L29/35 , C12N9/2428 , C12P7/06 , C12Y302/01003 , C13K1/06 , Y02E50/17
摘要： Presently provided are variant glucoamylases displaying altered properties, such as improved thermostability and/or specific activity. Also disclosed are DNA sequences coding for the variants, vectors and host cells incorporating the DNA sequence, enzyme compositions, and methods of using the variants in various applications.
摘要翻译：目前提供的是显示改变的性质的变体葡糖淀粉酶，例如改善的热稳定性和/或比活性。还公开了编码掺入DNA序列的变体，载体和宿主细胞的DNA序列，酶组合物和在各种应用中使用变体的方法。

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