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1. 发明授权

US06312916B1 Recombinant inactive core streptavidin mutants 失效
标题翻译：重组非活性核心链霉亲和素突变体
公开(公告)号：US06312916B1
公开(公告)日：2001-11-06
申请号：US08831399
申请日：1997-04-01
申请人： Erhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
发明人： Erhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
IPC分类号： G01N3353
CPC分类号： G01N33/5306 , A61K38/00 , C07K14/36 , C07K14/465
摘要： The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.
摘要翻译：本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白，其对生物素的结合亲和力降低，以及它们在测定分析物的方法中用作干扰消除试剂。在诊断测试中，例如免疫测定和核酸杂交测定。此外，本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白作为能够再生以结合生物素的体系的用途。用于分析生物素化分子，用于检查受体配体相互作用以及生物素化分子的亲和纯化。

2. 发明授权

US06391571B1 Recombinant inactive avidin mutants 有权
标题翻译：重组无活性抗生物素蛋白突变体
公开(公告)号：US06391571B1
公开(公告)日：2002-05-21
申请号：US09366862
申请日：1999-08-04
申请人： Erhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
发明人： Erhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
IPC分类号： G01N3353
CPC分类号： C07K14/36 , A61K38/00 , C07K14/465 , G01N33/5306
摘要： The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.
摘要翻译：本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白，其对生物素的结合亲和力降低，以及它们在测定分析物的方法中用作干扰消除试剂。在诊断测试中，例如免疫测定和核酸杂交测定。此外，本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白作为能够再生以结合生物素的体系的用途。用于分析生物素化分子，用于检查受体配体相互作用以及生物素化分子的亲和纯化。

3. 发明授权

US06417331B1 Recombinant inactive core streptavidin mutants 有权
标题翻译：重组非活性核心链霉亲和素突变体
公开(公告)号：US06417331B1
公开(公告)日：2002-07-09
申请号：US09368772
申请日：1999-08-05
申请人： Eberhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
发明人： Eberhard Kopetzki , Rainer Müller , Richard Engh , Urban Schmitt , Arno Deger , Hans Brandstetter
IPC分类号： C07K1400
CPC分类号： G01N33/5306 , A61K38/00 , C07K14/36 , C07K14/465
摘要： The present invention concerns muteins of avidin and streptavidin with a reduced binding affinity for biotin as well as their use as interference elimination reagents in methods for the determination of an analyte e.g. in diagnostic tests such as for example immunoassays and nucleic acid hybridization assays. In addition the invention concerns the use of muteins of avidin and streptavidin as systems capable of regeneration for binding biotin e.g. for the analysis of biotinylated molecules, for examining receptor ligand interactions as well as for the affinity purification of biotinylated molecules.
摘要翻译：本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白，其对生物素的结合亲和力降低，以及它们在测定分析物的方法中用作干扰消除试剂。在诊断测试中，例如免疫测定和核酸杂交测定。此外，本发明涉及抗生物素蛋白和链霉抗生物素蛋白的突变蛋白作为能够再生以结合生物素的体系的用途。用于分析生物素化分子，用于检查受体配体相互作用以及生物素化分子的亲和纯化。

4. 发明授权

US09261510B2 Detection of primary infections with pathogens 有权
标题翻译：检测病原体原发感染
公开(公告)号：US09261510B2
公开(公告)日：2016-02-16
申请号：US12104751
申请日：2008-04-17
申请人： Christian Schotz , Elke Faatz , Peter Schaarschmidt , Urban Schmitt
发明人： Christian Schotz , Elke Faatz , Peter Schaarschmidt , Urban Schmitt
IPC分类号： C07K14/20 , C07K14/245 , C07K14/45 , C07K14/47 , C12N15/62 , G01N33/68
CPC分类号： G01N33/6854 , C07K14/20 , C07K14/245 , C07K14/45 , C07K14/47 , C07K2319/70 , C12N15/62 , C12N2710/16122 , C12N2710/16134 , G01N2333/02 , G01N2333/035 , G01N2333/04 , G01N2333/045 , G01N2333/05 , G01N2333/19 , G01N2333/45
摘要： The present invention relates to fusion proteins suitable as test antigens in the detection of infections with pathogens, particularly of primary infections with pathogens. Further, the invention relates to methods for detecting and differentially determining antibodies, particularly IgM antibodies resulting from an infection with a pathogenic organism. Furthermore, test reagents for carrying out these methods are provided.
摘要翻译：本发明涉及适合作为检测抗原的融合蛋白，用于检测病原体的感染，特别是病原体的初次感染。此外，本发明涉及用于检测和差异测定抗体的方法，特别是由致病性生物体的感染产生的IgM抗体。此外，提供了用于实施这些方法的测试试剂。

5. 发明授权

US08790659B2 SlpA as a tool for recombinant protein and enzyme technology 有权
标题翻译： SlpA作为重组蛋白和酶技术的工具
公开(公告)号：US08790659B2
公开(公告)日：2014-07-29
申请号：US13569353
申请日：2012-08-08
申请人： Christian Scholz , Elke Faatz , Urban Schmitt , Peter Schaarschmidt
发明人： Christian Scholz , Elke Faatz , Urban Schmitt , Peter Schaarschmidt
IPC分类号： A61K39/245
CPC分类号： C12N15/62 , C12N9/90 , G01N33/536 , G01N33/54393 , Y02A50/57
摘要： Disclosed are a recombinant DNA molecule encoding a fusion protein comprising a SlpA chaperone and a target polypeptide wherein human FK506 binding proteins (FKBPs) are excluded as target polypeptides, a corresponding expression vector encoding said fusion protein as well as host cells transformed with said expression vector. Also disclosed are a method for producing the fusion protein, a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide. A further aspect of the invention is the use of the recombinantly produced fusion protein, and a reagent kit containing a recombinantly produced fusion protein comprising a SlpA chaperone and a target polypeptide.
摘要翻译：公开了编码包含SlpA伴侣和靶多肽的融合蛋白的重组DNA分子，其中人FK506结合蛋白（FKBP）被排除为靶多肽，编码所述融合蛋白的相应表达载体以及用所述表达载体转化的宿主细胞。还公开了产生融合蛋白的方法，重组产生的包含SlpA伴侣和靶多肽的融合蛋白。本发明的另一方面是使用重组产生的融合蛋白，以及含有重组产生的包含SlpA伴侣和靶多肽的融合蛋白的试剂盒。

6. 发明授权

US6096319A Recombinant antigen from the NS3 region of the hepatitis C virus 失效
标题翻译：来自丙型肝炎病毒NS3区域的重组抗原
公开(公告)号：US6096319A
公开(公告)日：2000-08-01
申请号：US511759
申请日：1995-08-07
申请人： Christoph Seidel , Ursula-Henrike Wienhues , Urban Schmitt , Manfred Motz , Michael Wiedmann , Barbara Upmeier , Erwin Soutschek
发明人： Christoph Seidel , Ursula-Henrike Wienhues , Urban Schmitt , Manfred Motz , Michael Wiedmann , Barbara Upmeier , Erwin Soutschek
IPC分类号： G01N33/53 , A61K38/00 , A61K39/29 , A61P1/16 , A61P31/12 , A61P31/14 , C07H21/04 , C07K14/02 , C07K14/10 , C07K14/18 , C07K16/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/40 , C12N15/51 , C12P21/02 , C12P21/04 , C12R1/19 , G01N33/532 , G01N33/537 , G01N33/569 , G01N33/576 , G01N33/68 , C12Q1/70
CPC分类号： C07K14/005 , G01N33/56983 , G01N33/5767 , A61K38/00 , C12N2770/24222 , Y10S436/82
摘要： The present invention concerns a polypeptide which is composed of the amino acids 1207.+-.10 to 1488.+-.10 of a hepatitis C virus and of less than 20 foreign amino acids and the use of this polypeptide as an antigen in an immunological test.
摘要翻译：本发明涉及由丙型肝炎病毒的氨基酸1207 +/- 10〜1488 +/- 10和少于20种外来氨基酸组成的多肽，以及在免疫学试验中使用该多肽作为抗原。

7. 发明授权

US5277589A Process for the determination of antibodies 失效
标题翻译：抗体测定方法
公开(公告)号：US5277589A
公开(公告)日：1994-01-11
申请号：US663759
申请日：1991-03-01
申请人： Urban Schmitt , Wolfgang Rudinger , Gertraud Ehrlich-Weinreich
发明人： Urban Schmitt , Wolfgang Rudinger , Gertraud Ehrlich-Weinreich
IPC分类号： G01N33/68 , G01N33/543 , C07K13/00 , C12Q1/00 , G01N33/564
CPC分类号： G01N33/686 , Y10S530/81 , Y10S530/866
摘要： For the determination of antibodies based on an immunoassay technique by incubation with at least three receptors R.sub.1, R.sub.2 and R.sub.3 which are present dissolved in a liquid phase and of which R.sub.1 is an antigen which is capable of being specifically bound to the antibody to be determined, R.sub.2 mediates the binding to the solid phase and R.sub.3 carries a label, separation of the complex which forms from the solution by binding to a solid phase and measurement of the label in one of the phases, a conjugate is used as the receptor R.sub.2 composed of a receptor capable of specific binding to R.sub.1 and a substance S.sub.1, which can be specifically bound, and a conjugate of a receptor which can specifically bind to R.sub.1 and a label is used as R.sub.3, wherein the immobilization of the complex which forms is mediated by binding to a component of the solid phase which can specifically bind S.sub.1.
摘要翻译：对于基于免疫测定技术的抗体的测定，其通过与存在于液相中的至少三种受体R1，R2和R3温育，其中R1是能够特异性结合待测定的抗体的抗原，R2介导与固相的结合，R3携带标记，通过结合固相分离与溶液形成的络合物，并在其中一个相中测量标记，使用缀合物作为受体R2组成可以特异性结合R1的受体和可以特异性结合的物质S1和可以特异性结合R1的受体的结合物和标记物用作R3，其中形成的络合物的固定被介导通过与可以特异性结合S1的固相的组分结合。

8. 发明授权

US07947494B2 Fusion protein comprising an E. coli chaperone protein and a human chaperone protein 有权
标题翻译：包含大肠杆菌伴侣蛋白和人伴侣蛋白的融合蛋白
公开(公告)号：US07947494B2
公开(公告)日：2011-05-24
申请号：US12166574
申请日：2008-07-02
申请人： Christian Scholz , Elke Faatz , Peter Schaarschmidt , Urban Schmitt
发明人： Christian Scholz , Elke Faatz , Peter Schaarschmidt , Urban Schmitt
IPC分类号： C12N15/00 , C07H21/04
CPC分类号： C12N9/90 , C07K2319/35
摘要： The invention discloses the cloning, expression and uses of a chimeric fusion protein with superior chaperone and folding activities compared to the wild type chaperones. This invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for an FK506 binding protein (FKBP) or an FK506-binding-protein-like domain (FKBP-like domain). In particular, this invention relates to a chimeric fusion protein encoded by a recombinant DNA molecule comprising nucleotide sequences coding for a polypeptide binding segment of a non-human chaperone protein and nucleotide sequences coding for a human FKBP type peptidyl-prolyl-cis/trans isomerase (PPIase), methods of producing these chimeric fusion proteins and their uses as folding helpers in the production of other proteins and in the process of the production of vaccines or pharmaceuticals, and as folding helpers for performing immunoassays.
摘要翻译：本发明公开了与野生型伴侣相比，具有优异的伴侣和折叠活性的嵌合融合蛋白的克隆，表达和用途。本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码FK506结合蛋白（FKBP）或FK506-结合蛋白 - 像域（FKBP样域）。特别地，本发明涉及由重组DNA分子编码的嵌合融合蛋白，其包含编码非人伴侣蛋白多肽结合片段的核苷酸序列和编码人FKBP型肽基 - 脯氨酰 - 顺/反异构酶的核苷酸序列（PPIase），生产这些嵌合融合蛋白的方法及其在生产其他蛋白质和疫苗或药物生产过程中作为折叠助剂的用途，以及用于进行免疫测定的折叠助剂。

9. 发明申请

US20050095584A1 Method for determining early HCV seroconversion 审中-公开
标题翻译：确定早期HCV血清学转换的方法
公开(公告)号：US20050095584A1
公开(公告)日：2005-05-05
申请号：US10802891
申请日：2004-03-17
申请人： Christoph Seidel , Ursula-Henrike Wienhues , Urban Schmitt , Manfred Motz , Michael Wiedmann , Barbara Upmeier , Erwin Soutschek
发明人： Christoph Seidel , Ursula-Henrike Wienhues , Urban Schmitt , Manfred Motz , Michael Wiedmann , Barbara Upmeier , Erwin Soutschek
IPC分类号： G01N33/53 , A61K38/00 , A61K39/29 , A61P1/16 , A61P31/12 , A61P31/14 , C07H21/04 , C07K14/02 , C07K14/10 , C07K14/18 , C07K16/00 , C12N1/15 , C12N1/19 , C12N1/21 , C12N5/10 , C12N15/09 , C12N15/40 , C12N15/51 , C12P21/02 , C12P21/04 , C12R1/19 , G01N33/532 , G01N33/537 , G01N33/569 , G01N33/576 , G01N33/68 , C12Q1/70 , C12N15/86 , G01N33/48 , G01N33/50 , G06F19/00
CPC分类号： C07K14/005 , A61K38/00 , C12N2770/24222 , G01N33/56983 , G01N33/5767 , Y10S436/82
摘要： The present invention concerns a polypeptide which is composed of the amino acids 1207±10 to 1488±10 of a hepatitis C virus and of less than 20 foreign amino acids and the use of this polypeptide as an antigen in an immunological test.
摘要翻译：本发明涉及由免疫学试验中由丙型肝炎病毒的氨基酸1207±10至1488±10和小于20个外来氨基酸组成的多肽和该多肽作为抗原的用途。

10. 发明授权

US06645732B2 Antigen-specific IgG detection 有权
标题翻译：抗原特异性IgG检测
公开(公告)号：US06645732B2
公开(公告)日：2003-11-11
申请号：US09319080
申请日：1999-11-08
申请人： Elke Faatz , Urban Schmitt
发明人： Elke Faatz , Urban Schmitt
IPC分类号： G01N3353
CPC分类号： G01N33/543 , G01N33/6854 , Y10S435/965 , Y10S530/866
摘要： The invention concerns a method for the determination of antigen-specific antibodies of the immunoglobulin G class in the presence of immunoglobulins of the M class in body fluids by incubation with at least two different receptors R1 and R2 and optionally additional receptors, an essential component of R2 being a binding partner in monomeric form, a reagent for determining an antigen-specific antibody of the immunoglobulin G class as well as the use of binding partners in monomeric form for the determination of an antigen-specific antibody of the immunoglobulin G class.
摘要翻译：本发明涉及通过与至少两种不同的受体R 1和R 2以及任选的另外的受体（其中的另外的受体）的基本成分一起温育，在体液中在M类的免疫球蛋白存在下，测定免疫球蛋白G类的抗原特异性抗体的方法 R2是单体形式的结合配偶体，用于测定免疫球蛋白G类抗原特异性抗体的试剂以及单体形式的结合配偶体用于测定免疫球蛋白G类抗原特异性抗体的用途。

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