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1. 发明授权

US4931385A Enzyme immunoassays and immunologic reagents 失效
标题翻译：酶免疫测定和免疫试剂
公开(公告)号：US4931385A
公开(公告)日：1990-06-05
申请号：US275656
申请日：1988-11-21
申请人： Elliott Block , Izak Bahar , Frank Cole , Cheryl A. Eaton , Wendy Jones , Eric Sigillo , Mary Coseo , Nancy J. Cicia , L. Edward Cannon , Walter Cantarow
发明人： Elliott Block , Izak Bahar , Frank Cole , Cheryl A. Eaton , Wendy Jones , Eric Sigillo , Mary Coseo , Nancy J. Cicia , L. Edward Cannon , Walter Cantarow
IPC分类号： G01N33/535 , G01N33/543
CPC分类号： G01N33/535 , G01N33/54393 , Y10S435/805 , Y10S435/81 , Y10S435/975 , Y10S436/808 , Y10S436/81 , Y10S436/814 , Y10S436/818 , Y10S436/826
摘要： Detection of bindable substances such as antibodies and antigens using enzyme linked immunosorbent assays having particular utility in home diagnostic applications. The preferred implementation of the invention is characterized by the steps of admixing a sample suspected of containing the bindable substance to be detected with an antibody-enzyme conjugate, immersing an antibody coated solid support into the mixture and then exposing the coated support to an activated chromogenic solution. The conjugate for use in the home diagnostic assay is preferably contained within a lyophilized mixture. The lyophilized mixture contains components which preserve the antibody-conjugate's reactivity and immunologic binding specificity even if the lyophilized mixture had been subjected to hot, humid environmental conditions. Active components in the lyophilized mixture include polyethylene glycol, sugars, and surfactant. The antibody coated support is treated with an improved blocking solution containing a blocking agent such as bovine serum albumin or milk protein in admixture with a sugar. The blocking solution prevents nonspecific binding of immunologic reagents to the support and protects the antibody on the support from loss of reactivity and immunologic binding specificity even if the antibody coated support had been subjected to hot, humid environmental conditions.
摘要翻译：使用在家庭诊断应用中具有特殊用途的酶联免疫吸附测定法检测可结合物质如抗体和抗原。本发明的优选实施方案的特征在于将怀疑含有待检测的可结合物质的样品与抗体 - 酶结合物混合，将抗体包被的固体支持物浸入混合物中，然后将包被的载体暴露于活化的显色解。用于家庭诊断测定的缀合物优选包含在冻干混合物中。冻干混合物含有保护抗体缀合物的反应性和免疫结合特异性的成分，即使冻干混合物已经经受炎热，潮湿的环境条件。冻干混合物中的活性组分包括聚乙二醇，糖和表面活性剂。抗体包被的载体用含有与糖混合的封闭剂如牛血清白蛋白或乳蛋白的改进的封闭溶液处理。阻断溶液防止免疫试剂与载体的非特异性结合，并保护载体上的抗体免受反应性和免疫结合特异性的损失，即使抗体包被的载体已经经受炎热，潮湿的环境条件。

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