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1. 发明授权

US5202267A Sol capture immunoassay kit and procedure 失效
标题翻译：溶胶捕获免疫测定试剂盒和程序
公开(公告)号：US5202267A
公开(公告)日：1993-04-13
申请号：US177114
申请日：1988-04-04
申请人： Charles C. Ditlow , L. Edward Cannon , Francis X. Cole , Gene A. Davis , Eric C. Sigillo , Alicia G. Danti
发明人： Charles C. Ditlow , L. Edward Cannon , Francis X. Cole , Gene A. Davis , Eric C. Sigillo , Alicia G. Danti
IPC分类号： G01N33/53 , G01N33/543 , G01N33/58
CPC分类号： G01N33/585 , Y10S436/824 , Y10S436/825 , Y10T436/25375 , Y10T436/255
摘要： An immunoassay procedure for detection of analytes in urine wherein an immunological reaction is conducted in an aqueous phase containing the urine and a filterable immunocomposite containing an inherently colored gold sol particle is formed if the assay is positive. The colored, gold sol particle containing immunocomposite is collected for direct visual observation on a filter element. False positive readings are minimized by (1) removing contaminants from the urine to prevent clogging of the filter element and non-specific binding of unreacted reactants thereto by contacting the urine with a porous contact element having a detergent material dispersed on its external and interstitial surfaces; (2) preventing non-specific binding of unreacted immunoreactive substances to the filter element by blocking the latter with polyvinyl pyrrolidone; and/or (3) including a green dye material in the aqueous reaction phase to improve the optical distinction after washing the collection filter between negative and positive results on the filter.
摘要翻译：用于检测尿液中分析物的免疫测定方法，其中在含有尿液的水相中进行免疫反应，并且如果测定为阳性，则形成含有固有着色的金溶胶颗粒的可过滤的免疫复合物。收集含有免疫复合物的彩色金溶胶颗粒，以便在过滤元件上进行直观目视观察。通过（1）从尿中除去污染物以防止过滤元件堵塞和未反应的反应物的非特异性结合使假阳性读数最小化，使尿与具有分散在其外部和间隙表面上的洗涤剂材料的多孔接触元件接触 ; （2）通过用聚乙烯吡咯烷酮封闭后者，防止未反应的免疫反应性物质与过滤元件的非特异性结合; 和/或（3）在水性反应相中包括绿色染料材料，以改善在过滤器上的负和正结果之后洗涤收集过滤器之后的光学区别。

2. 发明授权

US6083760A Sol capture immunoassay kit and procedure 失效
标题翻译：溶胶捕获免疫测定试剂盒和程序
公开(公告)号：US6083760A
公开(公告)日：2000-07-04
申请号：US998869
申请日：1992-12-30
申请人： Charles C. Ditlow , L. Edward Cannon , Francis X. Cole , Gene A. Davis , Eric C. Sigillo , Alicia G. Danti
发明人： Charles C. Ditlow , L. Edward Cannon , Francis X. Cole , Gene A. Davis , Eric C. Sigillo , Alicia G. Danti
IPC分类号： G01N33/53 , G01N33/543 , G01N33/58 , B01D37/02
CPC分类号： G01N33/585 , Y10S436/824 , Y10S436/825 , Y10T436/25375 , Y10T436/255
摘要： An immunoassay procedure for detection of analytes in urine wherein an immunological reaction is conducted in an aqueous phase containing the urine and a filterable immunocomposite containing an inherently colored gold sol particle is formed if the assay is positive. The colored, gold sol particle containing immunocomposite is collected for direct visual observation on a filter element. False positive readings are minimized by (1) removing contaminants from the urine to prevent clogging of the filter element and non-specific binding of unreacted reactants thereto by contacting the urine with a porous contact element having a detergent material dispersed on its external and interstitial surfaces; (2) preventing non-specific binding of unreacted immunoreactive substances to the filter element by blocking the latter with polyvinyl pyrrolidone; and/or (3) including a green dye material in the aqueous reaction phase to improve the optical distinction after washing the collection filter between negative and positive results on the filter.
摘要翻译：用于检测尿液中分析物的免疫测定方法，其中在含有尿液的水相中进行免疫反应，并且如果测定为阳性，则形成含有固有着色的金溶胶颗粒的可过滤的免疫复合物。收集含有免疫复合物的彩色金溶胶颗粒，以便在过滤元件上进行直观目视观察。通过（1）从尿中除去污染物以防止过滤元件堵塞和未反应的反应物的非特异性结合使假阳性读数最小化，通过使尿与具有分散在其外部和间隙表面上的洗涤剂材料的多孔接触元件接触 ; （2）通过用聚乙烯吡咯烷酮封闭后者，防止未反应的免疫反应性物质与过滤元件的非特异性结合; 和/或（3）在水性反应相中包括绿色染料材料，以改善在过滤器上的负和正结果之后洗涤收集过滤器之后的光学区别。

3. 发明授权

US5242804A Simultaneous dual analyte assay 失效
标题翻译：同时双重分析物测定
公开(公告)号：US5242804A
公开(公告)日：1993-09-07
申请号：US834990
申请日：1992-02-14
申请人： Izak Bahar , Francis X. Cole , L. Edward Cannon
发明人： Izak Bahar , Francis X. Cole , L. Edward Cannon
IPC分类号： G01N33/53 , G01N33/532 , G01N33/543 , G01N33/74
CPC分类号： G01N33/54306 , G01N33/743 , Y10S435/81 , Y10S435/972 , Y10S436/819
摘要： A simultaneous dual analyte assay for determining the fertile period of the human menstrual cycle. The assay utilizes a capture reaction component consisting of P-3-G immobilized on a microporous membrane, a blocking reaction component consisting of anti E.sub.1 -3-G antibody, a labelled reaction component consisting of gold particle labelled anti E.sub.1 -3-G antibody, and an ambifunctional reaction component consisting of a hybrid immunoreactive substance having an anti P-3-G antibody binding site and a plurality of E.sub.1 -3-G determinant binding sites. An aqueous sample containing P-3-G and E.sub.1 -3-G is contacted with the components and the assay is calibrated to provide a positive assay result only when the concentration of P-3-G in the sample is less than a predetermined concentration and the concentration of E.sub.1 -3-G in the sample is more than a preselected concentration whereby a visually inspectable quantity of an immunocomposite is produced that consists of both the P-3-G immobilized on the microporous membrane and the gold labelled anti E.sub.1 -3-G antibody.
摘要翻译：用于确定人类月经周期的可育期的同时双重分析物测定。该测定使用由固定在微孔膜上的P-3-G组成的捕获反应组分，由抗E1-3-G抗体组成的封闭反应组分，由金颗粒标记的抗E1-3-G抗体组成的标记反应组分，以及由具有抗P-3-G抗体结合位点和多个E1-3-G决定簇结合位点的杂交免疫反应物质组成的双功能反应组分。将含有P-3-G和E1-3-G的含水样品与组分接触，并且只有当样品中P-3-G的浓度小于预定浓度时，校准该测定以提供阳性测定结果并且样品中E1-3-G的浓度大于预选浓度，由此产生可视检测量的免疫复合物，其由固定在微孔膜上的P-3-G和标记为抗E1- 3-G抗体。

4. 发明授权

US4931385A Enzyme immunoassays and immunologic reagents 失效
标题翻译：酶免疫测定和免疫试剂
公开(公告)号：US4931385A
公开(公告)日：1990-06-05
申请号：US275656
申请日：1988-11-21
申请人： Elliott Block , Izak Bahar , Frank Cole , Cheryl A. Eaton , Wendy Jones , Eric Sigillo , Mary Coseo , Nancy J. Cicia , L. Edward Cannon , Walter Cantarow
发明人： Elliott Block , Izak Bahar , Frank Cole , Cheryl A. Eaton , Wendy Jones , Eric Sigillo , Mary Coseo , Nancy J. Cicia , L. Edward Cannon , Walter Cantarow
IPC分类号： G01N33/535 , G01N33/543
CPC分类号： G01N33/535 , G01N33/54393 , Y10S435/805 , Y10S435/81 , Y10S435/975 , Y10S436/808 , Y10S436/81 , Y10S436/814 , Y10S436/818 , Y10S436/826
摘要： Detection of bindable substances such as antibodies and antigens using enzyme linked immunosorbent assays having particular utility in home diagnostic applications. The preferred implementation of the invention is characterized by the steps of admixing a sample suspected of containing the bindable substance to be detected with an antibody-enzyme conjugate, immersing an antibody coated solid support into the mixture and then exposing the coated support to an activated chromogenic solution. The conjugate for use in the home diagnostic assay is preferably contained within a lyophilized mixture. The lyophilized mixture contains components which preserve the antibody-conjugate's reactivity and immunologic binding specificity even if the lyophilized mixture had been subjected to hot, humid environmental conditions. Active components in the lyophilized mixture include polyethylene glycol, sugars, and surfactant. The antibody coated support is treated with an improved blocking solution containing a blocking agent such as bovine serum albumin or milk protein in admixture with a sugar. The blocking solution prevents nonspecific binding of immunologic reagents to the support and protects the antibody on the support from loss of reactivity and immunologic binding specificity even if the antibody coated support had been subjected to hot, humid environmental conditions.
摘要翻译：使用在家庭诊断应用中具有特殊用途的酶联免疫吸附测定法检测可结合物质如抗体和抗原。本发明的优选实施方案的特征在于将怀疑含有待检测的可结合物质的样品与抗体 - 酶结合物混合，将抗体包被的固体支持物浸入混合物中，然后将包被的载体暴露于活化的显色解。用于家庭诊断测定的缀合物优选包含在冻干混合物中。冻干混合物含有保护抗体缀合物的反应性和免疫结合特异性的成分，即使冻干混合物已经经受炎热，潮湿的环境条件。冻干混合物中的活性组分包括聚乙二醇，糖和表面活性剂。抗体包被的载体用含有与糖混合的封闭剂如牛血清白蛋白或乳蛋白的改进的封闭溶液处理。阻断溶液防止免疫试剂与载体的非特异性结合，并保护载体上的抗体免受反应性和免疫结合特异性的损失，即使抗体包被的载体已经经受炎热，潮湿的环境条件。

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