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1. 发明授权

US08658398B2 Heterologous expression of termite cellulases yeast 有权
标题翻译：白蚁纤维素酶酵母的异源表达
公开(公告)号：US08658398B2
公开(公告)日：2014-02-25
申请号：US13003200
申请日：2009-07-07
申请人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John McBride , Chhayal Gandhi , Erin Wiswall
发明人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John McBride , Chhayal Gandhi , Erin Wiswall
IPC分类号： C12P19/14
CPC分类号： C12N9/2437 , C12N15/81 , C12N15/815 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
摘要翻译：本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。纤维素酶可以例如密码子优化并在酵母宿主细胞中表达，例如酵母酿酒酵母（Saccharomyces cerevisiae）。纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。因此，这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

2. 发明申请

US20120003701A1 Heterologous Expression of Termite Cellulases Yeast 有权
标题翻译：白蚁纤维素酵母的异源表达
公开(公告)号：US20120003701A1
公开(公告)日：2012-01-05
申请号：US13003200
申请日：2009-07-07
申请人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John Mcbride , Chhayal Gandhi , Erin Wiswall
发明人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John Mcbride , Chhayal Gandhi , Erin Wiswall
IPC分类号： C12P7/10 , C12P19/14 , C12N1/19 , C12N15/56 , C12N15/81
CPC分类号： C12N9/2437 , C12N15/81 , C12N15/815 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
摘要翻译：本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。纤维素酶可以例如密码子优化并在酵母宿主细胞中表达，例如酵母酿酒酵母（Saccharomyces cerevisiae）。纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。因此，这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

3. 发明授权

US08470592B2 Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1) 有权
标题翻译：裂殖壶菌纤维二糖水解酶I（Cbh 1）的分离和表征
公开(公告)号：US08470592B2
公开(公告)日：2013-06-25
申请号：US13003199
申请日：2009-07-07
申请人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
发明人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
IPC分类号： C12N15/00 , C12N9/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for the isolation and characterization of the cbh1 gene from Schizochytrium aggregatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbh1 in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbh1 gene can utilized in a consolidated bioprocessing system.
摘要翻译：本发明提供了来自裂殖壶菌的cbh1基因的分离和表征。特别地，本发明提供了裂殖壶菌综合征cbh1及其结构域，变体和衍生物的核酸和氨基酸序列。本发明还提供了在宿主细胞，包括酵母，例如酿酒酵母（Saccharomyces cerevisiae）的异源表达裂殖酵母凝集素Cbh1。裂殖酵母聚集体Cbh1在宿主细胞中的表达将增加纤维素消化并促进那些宿主细胞在纤维素基质上的乙醇生成。在某些实施方案中，酿酒酵母中的异源表达与其他已知或新鉴定的蔗糖分解酶的异源表达配合。因此，本发明还提供了新颖的裂殖壶菌聚集体Cbh1基因可以用于固结的生物处理系统中。

4. 发明申请

US20110312054A1 Isolation and Characterization of Schizochytrium Aggregatum Cellobiohydrolase I (CBH I) 有权
标题翻译：裂殖壶菌纤维二糖水解酶I（CBH I）的分离和表征
公开(公告)号：US20110312054A1
公开(公告)日：2011-12-22
申请号：US13003199
申请日：2009-07-07
申请人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
发明人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
IPC分类号： C12P7/10 , C12N1/21 , C12N15/63 , C12N1/19 , C12N9/42 , C12N15/56
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for the isolation and characterization of the cbh1 gene from Schizochytrium aggregatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbh1 in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbh1 gene can utilized in a consolidated bioprocessing system.
摘要翻译：本发明提供了来自裂殖壶菌的cbh1基因的分离和表征。特别地，本发明提供了裂殖壶菌综合征cbh1及其结构域，变体和衍生物的核酸和氨基酸序列。本发明还提供了在宿主细胞，包括酵母，例如酿酒酵母（Saccharomyces cerevisiae）的异源表达裂殖酵母凝集素Cbh1。裂殖酵母聚集体Cbh1在宿主细胞中的表达将增加纤维素消化并促进那些宿主细胞在纤维素基质上的乙醇生成。在某些实施方案中，酿酒酵母中的异源表达与其他已知或新鉴定的蔗糖分解酶的异源表达配合。因此，本发明还提供了新颖的裂殖壶菌聚集体Cbh1基因可以用于固结的生物处理系统中。

5. 发明授权

US09315833B2 Yeast cells expressing an exogenous cellulosome and methods of using the same 有权
标题翻译：表达外源性纤维素酶的酵母细胞及其使用方法
公开(公告)号：US09315833B2
公开(公告)日：2016-04-19
申请号：US13201257
申请日：2010-02-18
申请人： John McBride , Mark Mellon , Vineet Rajgarhia , Elena E. Brevnova , Erin Wiswall , David A. Hogsett , Danie LaGrange , Shaunita Rose , Emile Van Zyl
发明人： John McBride , Mark Mellon , Vineet Rajgarhia , Elena E. Brevnova , Erin Wiswall , David A. Hogsett , Danie LaGrange , Shaunita Rose , Emile Van Zyl
IPC分类号： C12P7/10 , C07K14/33 , C12N15/81 , C12N9/24 , C12N9/42
CPC分类号： C12P7/10 , C07K14/33 , C07K2319/035 , C07K2319/70 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N15/81 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , Y02E50/16 , Y02P20/52
摘要： The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.
摘要翻译：本发明涉及在微生物中异源纤维素酶体的工程和表达，以促进生物质转化为有用的产物。在一些实施方案中，本发明涉及形成纤维素酶的核的骨架蛋白的表达。纤维素酶或其他生物降解酶可以通过泊松特蛋白结构域 - 凝聚素结构域相互作用而非共价连接至支架蛋白。

6. 发明申请

US20120142046A1 Yeast Cells Expressing An Exogenous Cellulosome And Methods of Using The Same 有权
标题翻译：表达外源性纤维素酶的酵母细胞及其使用方法
公开(公告)号：US20120142046A1
公开(公告)日：2012-06-07
申请号：US13201257
申请日：2010-02-18
申请人： John McBride , Mark Mellon , Vineet Rajgarhia , Elena E. Brevnova , Erin Wiswall , David A. Hogsett , Danie LaGrange , Shaunita Rose , Emile Van Zyl
发明人： John McBride , Mark Mellon , Vineet Rajgarhia , Elena E. Brevnova , Erin Wiswall , David A. Hogsett , Danie LaGrange , Shaunita Rose , Emile Van Zyl
IPC分类号： C12P7/10 , C12P39/00 , C12N9/96 , C12P1/00 , C12N1/19 , C12N1/13
CPC分类号： C12P7/10 , C07K14/33 , C07K2319/035 , C07K2319/70 , C12N9/2402 , C12N9/2437 , C12N9/2445 , C12N9/248 , C12N15/81 , C12Y302/01004 , C12Y302/01008 , C12Y302/01021 , C12Y302/01037 , C12Y302/01091 , Y02E50/16 , Y02P20/52
摘要： The present invention relates to the engineering and expression of heterologous cellulosomes in microorganisms in order to facilitate the conversion of biomass to useful products. In some embodiments, the invention relates to the expression of scaffoldin proteins which form the nucleus of a cellulosome. Cellulases or other biomass-degrading enzymes can be non-covalently linked to the scaffoldin protein by virtue of a dockerin domain-cohesin domain interaction.
摘要翻译：本发明涉及在微生物中异源纤维素酶体的工程和表达，以促进生物质转化为有用的产物。在一些实施方案中，本发明涉及形成纤维素酶的核的骨架蛋白的表达。纤维素酶或其他生物降解酶可以通过泊松特蛋白结构域 - 凝聚素结构域相互作用而非共价连接至支架蛋白。

7. 发明授权

US09102955B2 Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose 有权
标题翻译：酵母表达纤维素酶用于同时糖化和发酵使用纤维素
公开(公告)号：US09102955B2
公开(公告)日：2015-08-11
申请号：US13130549
申请日：2009-11-23
申请人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
发明人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
IPC分类号： C12N1/19 , C12P7/10 , C12N15/80 , C12P7/14 , C12P39/00 , C12N9/42
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.
摘要翻译：包含表达异源纤维素酶的克氏杆菌的宿主细胞从纤维素产生乙醇此外，可以将表达不同杂种纤维素酶的多个宿主细胞共同共培养并用于从纤维素产生乙醇可以使用酵母菌株的重组酵母菌株和共培养物自己生产乙醇，也可以与外加纤维素酶组合使用，以提高发酵和发酵过程的效率。

8. 发明申请

US20120129229A1 Yeast Expressing Cellulases for Simultaneous Saccharification and Fermentation Using Cellulose 有权
标题翻译：酵母表达纤维素酶用于同时糖化和发酵使用纤维素
公开(公告)号：US20120129229A1
公开(公告)日：2012-05-24
申请号：US13130549
申请日：2009-11-23
申请人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
发明人： John McBride , Elena Brevnova , Mark Mellon , Allan Froehlich , Kristen Deleault , Vineet Rajgarhia , Riaan Den Haan , Merja Penttila , Marja Ilmen , Matti Siika-Aho , Jaana Uusitalo , Emily A. Stonehouse , Alan Gilbert , Haowen Xu , Deidre Willes , John Bardsley , Anu Koivula , Sanni Voutilainen
IPC分类号： C12N1/19 , C12P19/02
CPC分类号： C12P7/10 , C12N9/2437 , C12N9/2445 , C12N15/80 , C12P7/14 , C12P39/00 , C12Y302/01004 , C12Y302/01021 , C12Y302/01091 , Y02E50/16 , Y02E50/17
摘要： Host cells, comprising Kluveryomyces expressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes
摘要翻译：包含表达异源纤维素酶的克氏杆菌的宿主细胞从纤维素产生乙醇此外，可以将表达不同杂种纤维素酶的多个宿主细胞共同共培养并用于从纤维素产生乙醇可以使用酵母菌株的重组酵母菌株和共培养物自己生产乙醇，也可以与外加的纤维素酶组合使用，以提高发酵和发酵过程的效率

9. 发明申请

US20120149077A1 Mesophilic and Thermophilic Organisms Modified to Produce Acrylate, and Methods of Use Thereof 审中-公开
标题翻译：修饰生产丙烯酸酯的嗜温嗜热生物及其使用方法
公开(公告)号：US20120149077A1
公开(公告)日：2012-06-14
申请号：US13256297
申请日：2010-03-12
申请人： Arthur J. Shaw, IV , Vineet Rajgarhia
发明人： Arthur J. Shaw, IV , Vineet Rajgarhia
IPC分类号： C12P7/40 , C12N15/74 , C12N1/21
CPC分类号： C12P7/40 , C12N9/0006 , C12N9/1029 , C12N9/1217 , C12N15/63 , C12P1/04 , C12R1/01
摘要： The present invention provides for novel metabolic pathways leading to acrylate formation in a consolidated bio-processing system (CBP) where lignocellulosic biomass is efficiently converted to acrylate. In one such metabolic pathway, pyruvate is converted to lactate, which is converted to lactoyol-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In another such metabolic pathway, pyruvate is converted to L-α-alanine, which is converted to L-aspartate, which is converted to β-alanine, which is converted to β-alanyl-CoA, which is converted to acryloyl-CoA, and which is finally converted to acrylate. In yet another metabolic pathway, pyruvate is converted to lactate, and then lactate is converted directly to acrylate. In certain aspects, the invention provides for heterologous expression of one or more enzymes in a mesophilic or thermophilic organism, such as Thermoanaerobacterium saccharolyticum or Clostridium thermocellutn, where the one or more enzymes functions within a novel metabolic pathway as described above to convert pyruvate to acrylate via lactate, or via β alanine and acryloyl-CoA.
摘要翻译：本发明提供了导致在固体生物处理系统（CBP）中形成丙烯酸酯的新型代谢途径，其中木质纤维素生物质被有效地转化成丙烯酸酯。在一种这样的代谢途径中，将丙酮酸转化为乳酸盐，将其转化为乳糖醇CoA，将其转化为丙烯酰辅酶A，最后转化为丙烯酸酯。在另一个这样的代谢途径中，丙酮酸转化成L-谷氨酸，转化为L-天冬氨酸，转化为β-丙氨酸，转化为β-丙氨酰辅酶A，将其转化为丙烯酰 - CoA，最终转化为丙烯酸酯。在另一种代谢途径中，将丙酮酸转化成乳酸，然后将乳酸酯直接转化成丙烯酸酯。在某些方面，本发明提供了一种或多种酶在嗜温或嗜热生物体中的异源表达，所述生物体如热解酵母分解酶或热梭菌（Clostridium thermocellutn），其中一种或多种酶在如上所述的新型代谢途径中起作用以将丙酮酸转化为丙烯酸酯通过乳酸盐，或通过＆bgr; 丙氨酸和丙烯酰辅酶A。

10. 发明授权

US06485947B1 Production of lactate using crabtree negative organisms in varying culture conditions 有权
标题翻译：在不同的培养条件下使用crabtree负面生物生产乳酸盐
公开(公告)号：US06485947B1
公开(公告)日：2002-11-26
申请号：US09574873
申请日：2000-05-19
申请人： Vineet Rajgarhia , Vassily Hatzimanikatis , Stacey Olson , Ting Carlson , John N. Starr , Jeffrey J. Kolstad , Aharon Eyal
发明人： Vineet Rajgarhia , Vassily Hatzimanikatis , Stacey Olson , Ting Carlson , John N. Starr , Jeffrey J. Kolstad , Aharon Eyal
IPC分类号： C12P756
CPC分类号： C12N1/16 , C12P7/40 , C12P7/48 , C12P7/56 , C12P7/60 , Y02E50/17
摘要： Crabtree negative organisms such as Kluyveromyces, Pichia, Hansenula and Candida, are used to make selected organic products such as lactic acid. The organisms are cultured in a first culture medium that includes glucose, under conditions that promote cellular respiration. The organisms are then cultured under a second set of conditions that promote production of the selected organic product. The organisms preferably contain an exogenous lactate dehydrogenase gene.
摘要翻译： Crubtree阴性生物如克鲁维酵母，毕赤酵母，汉逊酵母和念珠菌用于制备选择的有机产物如乳酸。在促进细胞呼吸的条件下，生物体在包括葡萄糖的第一培养基中培养。然后在促进所选有机产物生产的第二组条件下培养生物体。生物体优选含有外源乳酸脱氢酶基因。

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