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1. 发明授权

US08470592B2 Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1) 有权
标题翻译：裂殖壶菌纤维二糖水解酶I（Cbh 1）的分离和表征
公开(公告)号：US08470592B2
公开(公告)日：2013-06-25
申请号：US13003199
申请日：2009-07-07
申请人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
发明人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
IPC分类号： C12N15/00 , C12N9/00 , C07H21/04 , C07K14/00
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for the isolation and characterization of the cbh1 gene from Schizochytrium aggregatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbh1 in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbh1 gene can utilized in a consolidated bioprocessing system.
摘要翻译：本发明提供了来自裂殖壶菌的cbh1基因的分离和表征。特别地，本发明提供了裂殖壶菌综合征cbh1及其结构域，变体和衍生物的核酸和氨基酸序列。本发明还提供了在宿主细胞，包括酵母，例如酿酒酵母（Saccharomyces cerevisiae）的异源表达裂殖酵母凝集素Cbh1。裂殖酵母聚集体Cbh1在宿主细胞中的表达将增加纤维素消化并促进那些宿主细胞在纤维素基质上的乙醇生成。在某些实施方案中，酿酒酵母中的异源表达与其他已知或新鉴定的蔗糖分解酶的异源表达配合。因此，本发明还提供了新颖的裂殖壶菌聚集体Cbh1基因可以用于固结的生物处理系统中。

2. 发明申请

US20110312054A1 Isolation and Characterization of Schizochytrium Aggregatum Cellobiohydrolase I (CBH I) 有权
标题翻译：裂殖壶菌纤维二糖水解酶I（CBH I）的分离和表征
公开(公告)号：US20110312054A1
公开(公告)日：2011-12-22
申请号：US13003199
申请日：2009-07-07
申请人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
发明人： Elena E. Brevnova , Jim Flatt , Chhayal Gandhi , Vineet Rajgarhia , John McBride , Anne Warner
IPC分类号： C12P7/10 , C12N1/21 , C12N15/63 , C12N1/19 , C12N9/42 , C12N15/56
CPC分类号： C12N9/2437 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for the isolation and characterization of the cbh1 gene from Schizochytrium aggregatum. In particular, the present invention provides for the nucleic acid and amino acid sequences of Schizochytrium aggregatum cbh1, and domains, variants and derivatives thereof. The present invention further provides for the heterologous expression of Schizochytrium aggregatum Cbh1 in host cells, including yeast, e.g., Saccharomyces cerevisiae. Expression of Schizochytrium aggregatum Cbh1 in host cells will augment cellulose digestion and facilitate ethanol production by those host cells on cellulosic substrates. In certain embodiments, heterologous expression in Saccharomyces cerevisiae is in coordination with heterologous expression of other known, or newly identified saccharolytic enzymes. Therefore, the present invention also provides that the novel Schizochytrium aggregatum Cbh1 gene can utilized in a consolidated bioprocessing system.
摘要翻译：本发明提供了来自裂殖壶菌的cbh1基因的分离和表征。特别地，本发明提供了裂殖壶菌综合征cbh1及其结构域，变体和衍生物的核酸和氨基酸序列。本发明还提供了在宿主细胞，包括酵母，例如酿酒酵母（Saccharomyces cerevisiae）的异源表达裂殖酵母凝集素Cbh1。裂殖酵母聚集体Cbh1在宿主细胞中的表达将增加纤维素消化并促进那些宿主细胞在纤维素基质上的乙醇生成。在某些实施方案中，酿酒酵母中的异源表达与其他已知或新鉴定的蔗糖分解酶的异源表达配合。因此，本发明还提供了新颖的裂殖壶菌聚集体Cbh1基因可以用于固结的生物处理系统中。

3. 发明授权

US08658398B2 Heterologous expression of termite cellulases yeast 有权
标题翻译：白蚁纤维素酶酵母的异源表达
公开(公告)号：US08658398B2
公开(公告)日：2014-02-25
申请号：US13003200
申请日：2009-07-07
申请人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John McBride , Chhayal Gandhi , Erin Wiswall
发明人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John McBride , Chhayal Gandhi , Erin Wiswall
IPC分类号： C12P19/14
CPC分类号： C12N9/2437 , C12N15/81 , C12N15/815 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
摘要翻译：本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。纤维素酶可以例如密码子优化并在酵母宿主细胞中表达，例如酵母酿酒酵母（Saccharomyces cerevisiae）。纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。因此，这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

4. 发明申请

US20120003701A1 Heterologous Expression of Termite Cellulases Yeast 有权
标题翻译：白蚁纤维素酵母的异源表达
公开(公告)号：US20120003701A1
公开(公告)日：2012-01-05
申请号：US13003200
申请日：2009-07-07
申请人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John Mcbride , Chhayal Gandhi , Erin Wiswall
发明人： Elena E. Brevnova , Vineet Rajgarhia , Mark Mellon , Anne Warner , John Mcbride , Chhayal Gandhi , Erin Wiswall
IPC分类号： C12P7/10 , C12P19/14 , C12N1/19 , C12N15/56 , C12N15/81
CPC分类号： C12N9/2437 , C12N15/81 , C12N15/815 , C12P7/10 , C12P19/02 , C12P19/14 , C12Y302/01091 , Y02E50/16
摘要： The present invention provides for heterologous expression of termite and termite-associated symbiont cellulases. The cellulases can, for example, be codon-optimized and expressed in yeast host cells, such as the yeast Saccharomyces cerevisiae. The cellulases can also be co-expressed in host cells with other cellulases. The expression in such host cells of the termite and termite-associated symbiont cellulases, and variants and combinations thereof, result in yeast with improved cellulosic activity. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.
摘要翻译：本发明提供白蚁和白蚁相关共生体纤维素酶的异源表达。纤维素酶可以例如密码子优化并在酵母宿主细胞中表达，例如酵母酿酒酵母（Saccharomyces cerevisiae）。纤维素酶也可以与其他纤维素酶在宿主细胞中共表达。在白蚁和白蚁相关共生体纤维素酶的这种宿主细胞中的表达及其变体和组合导致具有改善的纤维素活性的酵母。因此，这些基因和表达系统对于有效和成本有效的整合生物处理系统是有用的。

5. 发明申请

US20110059485A1 Plasmids from Thermophilic Organisms, Vectors Derived Therefrom, and Uses Thereof 审中-公开
标题翻译：来自嗜热生物的质粒，衍生自其的载体及其用途
公开(公告)号：US20110059485A1
公开(公告)日：2011-03-10
申请号：US12677428
申请日：2008-09-10
申请人： Nicky Caiazza , Anne Warner , Chris Herring
发明人： Nicky Caiazza , Anne Warner , Chris Herring
IPC分类号： C12P21/02 , C07H21/04 , C12N15/63 , C12N15/70 , C12N15/74 , C12N1/21 , C12N1/19 , C12P19/34 , C12N15/64 , C07K14/00 , C12N9/16
CPC分类号： C12N15/70 , C12N15/74 , C12N15/81
摘要： The present invention is directed to a replicative, thermostable plasmid. In particular, the present invention is directed to a replicative, thermostable plasmid comprising a sequence derived from the pB6A plasmid and at least one functional unit comprising a sequence that is not found in plasmid pB6A.
摘要翻译：本发明涉及一种复制的热稳定性质粒。特别地，本发明涉及包含源自pB6A质粒的序列和包含在质粒pB6A中未发现的序列的至少一个功能单元的复制热稳定质粒。

6. 发明授权

US4551165A Mycorrhizal seed pellets 失效
标题翻译：菌根种子颗粒
公开(公告)号：US4551165A
公开(公告)日：1985-11-05
申请号：US486192
申请日：1983-04-18
申请人： Anne Warner
发明人： Anne Warner
IPC分类号： A01C1/06 , A01G1/04 , C05F11/02
CPC分类号： A01C1/06 , A01G1/048 , Y10S47/09
摘要： It has been a problem to pellet seeds with inoculum of the beneficial VA mycorrhizal fungus. Hitherto, clay or soil pellets have been tried but they are difficult to adjust to a suitable moisture content and the pellets are too heavy, and there is a problem in placement of the seed so as to ensure that the radicle will grow through the pellet and thereby pick up the desired fungal infection. It has now been found that satisfactory pellets can be made using a mixture of peat, preferably sphagnum moss peat (instead of soil), together with a binder, seed and the VA mycorrhizal fungus, and that even when the pellet is dried considerably the inoculum retains infectivity for at least 6 months, using sphagnum moss peat. The composition in compacted, sowing unit, e.g. pellet, form and a method of growing plant from seed are claimed. The invention is useful for improving the condition of poor soil, e.g. filled-in gravel pits or coal mine spoil tips.
摘要翻译：用有益的VA菌根真菌的接种物对种子进行沉淀是一个问题。迄今为止，已经尝试了粘土或土壤颗粒，但是它们难以适应于适当的水分含量，并且颗粒太重，并且存在种子放置的问题，以确保胚根通过颗粒生长，从而吸收所需的真菌感染。现在已经发现，可以使用泥炭，优选泥炭泥（代替土壤）和粘合剂，种子和VA菌根真菌的混合物制备令人满意的颗粒，并且即使当沉淀物被大量干燥时，接种物使用泥炭泥炭泥保持至少6个月的感染性。压实，播种单位中的组合物，例如要求从种子中种植植物的颗粒，形式和方法。本发明可用于改善贫瘠土壤的状况，例如，填充的砾石坑或煤矿破坏点。

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