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1. 发明授权

US10087421B2 Stem cell suspension 有权
公开(公告)号：US10087421B2
公开(公告)日：2018-10-02
申请号：US13883371
申请日：2011-11-09
申请人： Eiji Kobayashi , Tamaki Wada , Yasutaka Fujita , Norihiro Yoshinaga , Masako Doi , Yasuhiro Fujimoto , Takumi Teratani
发明人： Eiji Kobayashi , Tamaki Wada , Yasutaka Fujita , Norihiro Yoshinaga , Masako Doi , Yasuhiro Fujimoto , Takumi Teratani
IPC分类号： C12N5/071 , C07H3/04 , C12N5/0775 , A01N1/02 , A61K9/00 , A61K47/26 , A61K47/36 , A61K35/28 , C08B31/04 , C08B37/02
摘要： The present invention provides a mammalian stem cell suspension containing mammalian stem cells and at least one polysaccharide such as trehalose, and the like; a mammalian stem cell aggregation inhibitor containing polysaccharide such as trehalose, and the like; a method of suppressing aggregation of mammalian stem cells, containing suspending the mammalian stem cells in an aqueous physiological solution containing polysaccharide; an inhibitor of a decrease in the survival rate of mammalian stem cells containing polysaccharide such as trehalose and the like; a method of suppressing a decrease in the survival rate of mammalian stem cells, containing suspending the mammalian stem cells in an aqueous physiological solution containing polysaccharides, and the like.

2. 发明申请

US20130260461A1 STEM CELL SUSPENSION 审中-公开
标题翻译：干细胞悬浮液
公开(公告)号：US20130260461A1
公开(公告)日：2013-10-03
申请号：US13883371
申请日：2011-11-09
申请人： Eiji Kobayashi , Tamaki Wada , Yasutaka Fujita , Norihiro Yoshinaga , Masako Doi , Yasuhiro Fujimoto , Takumi Teratani
发明人： Eiji Kobayashi , Tamaki Wada , Yasutaka Fujita , Norihiro Yoshinaga , Masako Doi , Yasuhiro Fujimoto , Takumi Teratani
IPC分类号： C12N5/071 , C08B31/04 , C08B37/02 , C07H3/04
CPC分类号： C12N5/0667 , A01N1/0226 , A61K9/0019 , A61K35/28 , A61K47/26 , A61K47/36 , C07H3/04 , C08B31/04 , C08B37/0021 , C12N5/0663 , C12N2500/34
摘要： The present invention provides a mammalian stem cell suspension containing mammalian stem cells and at least one polysaccharide such as trehalose, and the like; a mammalian stem cell aggregation inhibitor containing polysaccharide such as trehalose, and the like; a method of suppressing aggregation of mammalian stem cells, containing suspending the mammalian stem cells in an aqueous physiological solution containing polysaccharide; an inhibitor of a decrease in the survival rate of mammalian stem cells containing polysaccharide such as trehalose and the like; a method of suppressing a decrease in the survival rate of mammalian stem cells, containing suspending the mammalian stem cells in an aqueous physiological solution containing polysaccharides, and the like.
摘要翻译：本发明提供了含有哺乳动物干细胞和至少一种多糖如海藻糖等的哺乳动物干细胞悬浮液; 含有海藻糖等多糖的哺乳动物干细胞聚集抑制剂等; 一种抑制哺乳动物干细胞聚集的方法，其包含将哺乳动物干细胞悬浮在含有多糖的生理水溶液中; 是含有海藻糖等多糖的哺乳动物干细胞存活率下降的抑制剂; 抑制哺乳动物干细胞的存活率降低的方法，其包含将哺乳动物干细胞悬浮在含有多糖的生理水溶液等中。

3. 发明授权

US07736893B2 Nanobio device of imitative anatomy structure 有权
标题翻译：模拟解剖结构的Nanobio装置
公开(公告)号：US07736893B2
公开(公告)日：2010-06-15
申请号：US11353603
申请日：2006-02-14
申请人： Masanao Munekane , Hiroyuki Wada , Kouji Iwasaki , Toshiaki Fujii , Takahiro Ochiya , Yusuke Yamamoto , Takumi Teratani
发明人： Masanao Munekane , Hiroyuki Wada , Kouji Iwasaki , Toshiaki Fujii , Takahiro Ochiya , Yusuke Yamamoto , Takumi Teratani
IPC分类号： C12N5/00
CPC分类号： G01N33/5088 , Y10T436/11
摘要： Objects to be achieved by the invention are to provide a nanobio device in which cultured cells are organized at a high-level in a state near in vivo, and to provide a method of using the nanobio device of imitative anatomy structure. The nanobio device of imitative anatomy structure of the invention is obtained by manufacturing a substrate with a bio-compatible substance and arranging a plurality of types of cells thereon in a desired array. A method of manufacturing a nanobio device in the invention includes a step of manufacturing a substrate for a nanobio device by a micromachine processing technique and a step of arranging a plurality of cultured cells on the substrate in a desired array with laser optical tweezers.
摘要翻译：通过本发明实现的目的是提供一种纳米生物装置，其中培养细胞在体内接近的状态下组织在高水平，并提供使用模拟解剖结构的纳米装置的方法。本发明的模拟解剖结构的纳米装置通过用生物相容性物质制造衬底并以期望的阵列布置多种类型的细胞而获得。在本发明中制造纳米装置的方法包括通过微机械加工技术制造纳米装置用基板的步骤，以及使用激光光学镊子以期望的阵列在基板上排列多个培养细胞的步骤。

4. 发明授权

US07332336B2 Methods for inducing differentiation of pluripotent cells 失效
标题翻译：诱导多能细胞分化的方法
公开(公告)号：US07332336B2
公开(公告)日：2008-02-19
申请号：US10789159
申请日：2004-02-27
申请人： Takahiro Ochiya , Takumi Teratani
发明人： Takahiro Ochiya , Takumi Teratani
IPC分类号： C12N5/00 , C12N5/08
CPC分类号： C12N5/067 , C12N2501/113 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/235 , C12N2501/237 , C12N2506/02 , C12N2533/54
摘要： The present invention provides methods for inducing the differentiation of pluripotent cells. The present inventors succeeded in differentiating hepatocytes from ES cells without EB formation, using simple adherent monoculturing in media comprising several growth factors in culture dishes with two different matrices.
摘要翻译：本发明提供诱导多能细胞分化的方法。本发明人在具有两种不同的基质的培养皿中，使用简单的粘附单培养在包含几种生长因子的培养基中的简单粘附单培养，成功地将肝细胞与ES细胞分化而不形成EB。

5. 发明申请

US20070186293A1 Rat embryonic stem cell 有权
标题翻译：大鼠胚胎干细胞
公开(公告)号：US20070186293A1
公开(公告)日：2007-08-09
申请号：US10591407
申请日：2005-03-01
申请人： Takumi Teratani , Takahiro Ochiya
发明人： Takumi Teratani , Takahiro Ochiya
IPC分类号： A01K67/027 , C12N5/06 , C40B40/02 , C40B40/08
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A01K2267/0393 , C12N5/0606 , C12N15/873 , C12N2501/235 , G01N33/5073
摘要： The present invention provides a rat embryonic stem cell characterized by having the following properties of (a) expressing Oct3/4 gene and Nanog gene, (b) positive for alkaline phosphatase activity, (c) having an embryoid body forming ability, (d) expressing SSEA (Stage-Specific Embryonic Antigen)-1 and SSEA-4, (e) having the same number of chromosomes as does a normal rat cell, (f) capable of being subcultured and holding the undifferentiated state, (g) having in vitro pluripotency, (h) having a potential to differentiate for cells of three embryonic germ lineages, (i) having teratoma formation ability, and (j) having an ability to produce a chimeric rat, a method of establishing the aforementioned rat embryonic stem cell and the like.
摘要翻译：本发明提供一种大鼠胚胎干细胞，其特征在于具有（a）表达Oct3 / 4基因和Nanog基因的以下性质，（b）碱性磷酸酶活性阳性，（c）具有胚状体形成能力，（d）表达SSEA（阶段特异性胚胎抗原）-1和SSEA-4，（e）具有与正常大鼠细胞相同数量的染色体，（f）能够被传代培养并保持未分化状态，（g）具有（h）具有分化为三种胚胎胚芽谱系的细胞的潜力，（i）具有畸胎瘤形成能力和（j）具有产生嵌合大鼠的能力的方法，建立上述大鼠胚胎干细胞的方法等等。

6. 发明申请

US20050042748A1 Methods for inducing differentiation of pluripotent cells 失效
标题翻译：诱导多能细胞分化的方法
公开(公告)号：US20050042748A1
公开(公告)日：2005-02-24
申请号：US10789159
申请日：2004-02-27
申请人： Takahiro Ochiya , Takumi Teratani
发明人： Takahiro Ochiya , Takumi Teratani
IPC分类号： A61K35/407 , A61P1/16 , C12N5/02 , C12N5/071 , C12N5/08
CPC分类号： C12N5/067 , C12N2501/113 , C12N2501/115 , C12N2501/119 , C12N2501/12 , C12N2501/235 , C12N2501/237 , C12N2506/02 , C12N2533/54
摘要： The object of the present invention is to provide methods for identifying factors involved in inducing the differentiation of pluripotent cells, and methods for using those factors. Using cDNA microarray technology, variations in gene expression between placebo-treated and CCl4-treated mice livers were analyzed 24 hours after treatment, and several growth factors were selected. The effects of matrix and growth factors on hepatocyte differentiation rates of ES cells were investigated. As a result, the present inventors succeeded in differentiating hepatocytes from ES cells without EB formation, using simple adherent monoculturing in media comprising several growth factors in culture dishes with two different matrices.
摘要翻译：本发明的目的是提供用于鉴定诱导多能细胞分化的因素的方法和使用这些因子的方法。使用cDNA微阵列技术，处理后24小时分析安慰剂处理和CCl4处理的小鼠肝脏基因表达变化，并选择几种生长因子。研究了基质和生长因子对ES细胞肝细胞分化率的影响。结果，本发明人成功地在没有EB形成的情况下使肝细胞与ES细胞分化，在具有两种不同基质的培养皿中使用包含几种生长因子的培养基中的简单粘附单培养。

7. 发明授权

US08628957B2 Rat embryonic stem cell 有权
标题翻译：大鼠胚胎干细胞
公开(公告)号：US08628957B2
公开(公告)日：2014-01-14
申请号：US13370795
申请日：2012-02-10
申请人： Takumi Teratani , Takahiro Ochiya
发明人： Takumi Teratani , Takahiro Ochiya
IPC分类号： C12N5/06 , C12N5/00 , A01K67/027
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A01K2267/0393 , C12N5/0606 , C12N15/873 , C12N2501/235 , G01N33/5073
摘要： The present invention provides a rat embryonic stem cell characterized by having the following properties of (a) expressing Oct3/4 gene and Nanog gene, (b) positive for alkaline phosphatase activity, (c) having an embryoid body forming ability, (d) expressing SSEA (Stage-Specific Embryonic Antigen)-1 and SSEA-4, (e) having the same number of chromosomes as does a normal rat cell, (f) capable of being subcultured and holding the undifferentiated state, (g) having in vitro pluripotency, (h) having a potential to differentiate for cells of three embryonic germ lineages, (i) having teratoma formation ability, and (j) having an ability to produce a chimeric rat, a method of establishing the aforementioned rat embryonic stem cell and the like.
摘要翻译：本发明提供一种大鼠胚胎干细胞，其特征在于具有（a）表达Oct3 / 4基因和Nanog基因的以下性质，（b）碱性磷酸酶活性阳性，（c）具有胚状体形成能力，（d）表达SSEA（阶段特异性胚胎抗原）-1和SSEA-4，（e）具有与正常大鼠细胞相同数量的染色体，（f）能够被传代培养并保持未分化状态，（g）具有（h）具有分化为三种胚胎胚芽谱系的细胞的潜力，（i）具有畸胎瘤形成能力和（j）具有产生嵌合大鼠的能力的方法，建立上述大鼠胚胎干细胞的方法等等。

8. 发明申请

US20060154235A1 Human hepatocyte-like cells and uses thereof 审中-公开
标题翻译：人肝细胞样细胞及其用途
公开(公告)号：US20060154235A1
公开(公告)日：2006-07-13
申请号：US11210337
申请日：2005-08-23
申请人： Takahiro Ochiya , Takumi Teratani
发明人： Takahiro Ochiya , Takumi Teratani
IPC分类号： C12Q1/00 , C12N5/08
CPC分类号： C12N5/067 , C12N2501/39 , C12Q1/18 , G01N33/5014 , G01N33/5038 , G01N33/5067 , G01N2800/08
摘要： The present invention provides modified methods of producing human hepatocyte-like cells which exhibit phenotypes more similar to those of human hepatocytes. The present invention also provides the human hepatocyte-like cells and uses thereof. The methods of the present invention comprises a prolonged incubation period and addition of dexamethasone to the culture medium. The methods of the inventors produce human hepatocyte-like cells whose morphology is more similar to that of human hepatocytes, compared with the conventional system. The cells produced have both morphological and functional features of primary culture cells from normal human liver, including cytochrome P450 (CYP), multi-drug resistance-associated protein (MRP), and multi-drug protein (MDR). The use of human hepatocyte-like cells of the present invention enables, without using any animal model, assessment of metabolism and hepatotoxicity of test compounds, which are drug candidates, and screening for therapeutic agents for hepatic diseases, inhibitors to hepatitis virus infection, and therapeutic agents for viral hepatitis.
摘要翻译：本发明提供了生产人肝细胞样细胞的修饰方法，其表现出与人肝细胞更相似的表型。本发明还提供人肝细胞样细胞及其用途。本发明的方法包括延长的潜伏期，并向培养基中加入地塞米松。与常规系统相比，本发明人的方法产生的人类肝细胞样细胞的形态与人肝细胞的形态更相似。所产生的细胞具有来自正常人肝脏的原代培养细胞的形态和功能特征，包括细胞色素P450（CYP），多药耐药相关蛋白（MRP）和多药物蛋白（MDR）。使用本发明的人类肝细胞样细胞能够在不使用任何动物模型的情况下评估作为药物候选物的测试化合物的代谢和肝毒性，以及筛选肝脏疾病的治疗剂，肝炎病毒感染的抑制剂和病毒性肝炎治疗剂。

9. 发明授权

US08137966B2 Rat embryonic stem cell 有权
标题翻译：大鼠胚胎干细胞
公开(公告)号：US08137966B2
公开(公告)日：2012-03-20
申请号：US10591407
申请日：2005-03-01
申请人： Takumi Teratani , Takahiro Ochiya
发明人： Takumi Teratani , Takahiro Ochiya
IPC分类号： C12N5/06 , C12N5/02 , A01N63/00
CPC分类号： A01K67/0275 , A01K2217/05 , A01K2227/105 , A01K2267/0393 , C12N5/0606 , C12N15/873 , C12N2501/235 , G01N33/5073
摘要： The present invention provides a rat embryonic stem cell characterized by having the following properties of (a) expressing Oct3/4 gene and Nanog gene, (b) positive for alkaline phosphatase activity, (c) having an embryoid body forming ability, (d) expressing SSEA (Stage-Specific Embryonic Antigen)-1 and SSEA-4, (e) having the same number of chromosomes as does a normal rat cell, (f) capable of being subcultured and holding the undifferentiated state, (g) having in vitro pluripotency, (h) having a potential to differentiate for cells of three embryonic germ lineages, (i) having teratoma formation ability, and (j) having an ability to produce a chimeric rat, a method of establishing the aforementioned rat embryonic stem cell and the like.
摘要翻译：本发明提供一种大鼠胚胎干细胞，其特征在于具有（a）表达Oct3 / 4基因和Nanog基因的以下性质，（b）碱性磷酸酶活性阳性，（c）具有胚状体形成能力，（d）表达SSEA（阶段特异性胚胎抗原）-1和SSEA-4，（e）具有与正常大鼠细胞相同数量的染色体，（f）能够被传代培养并保持未分化状态，（g）具有（h）具有分化为三种胚胎胚芽谱系的细胞的潜力，（i）具有畸胎瘤形成能力和（j）具有产生嵌合大鼠的能力的方法，建立上述大鼠胚胎干细胞的方法等等。

10. 发明申请

US20060188946A1 Nanobio device of imitative anatomy structure 有权
标题翻译：模拟解剖结构的Nanobio装置
公开(公告)号：US20060188946A1
公开(公告)日：2006-08-24
申请号：US11353603
申请日：2006-02-14
申请人： Masanao Munekane , Hiroyuki Wada , Kouji Iwasaki , Toshiaki Fujii , Takahiro Ochiya , Yusuke Yamamoto , Takumi Teratani
发明人： Masanao Munekane , Hiroyuki Wada , Kouji Iwasaki , Toshiaki Fujii , Takahiro Ochiya , Yusuke Yamamoto , Takumi Teratani
IPC分类号： G01N33/567 , G01N33/53
CPC分类号： G01N33/5088 , Y10T436/11
摘要： objects to be achieved by the invention are to provide a nanobio device in which cultured cells are organized at a high-level in a state near in vivo, and to provide a method of using the nanobio device of imitative anatomy structure. The nanobio device of imitative anatomy structure of the invention is obtained by manufacturing a substrate with a bio-compatible substance and arranging a plurality of types of cells thereon in a desired array. A method of manufacturing a nanobio device in the invention includes a step of manufacturing a substrate for a nanobio device by a micromachine processing technique and a step of arranging a plurality of cultured cells on the substrate in a desired array with laser optical tweezers.
摘要翻译：本发明要实现的目的是提供一种纳米生物装置，其中培养细胞在体内接近的状态下组织成高水平，并且提供使用模拟解剖结构的纳米装置的方法。本发明的模拟解剖结构的纳米装置通过用生物相容性物质制造衬底并以期望的阵列布置多种类型的细胞而获得。在本发明中制造纳米装置的方法包括通过微机械加工技术制造纳米装置用基板的步骤，以及使用激光光学镊子以期望的阵列在基板上排列多个培养细胞的步骤。

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