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1. 发明授权

US10167483B2 Herbicide resistance genes 有权
公开(公告)号：US10167483B2
公开(公告)日：2019-01-01
申请号：US14491197
申请日：2014-09-19
申请人： Dow AgroSciences LLC
发明人： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald Merlo , Jayakumar Pon Samuel , Gaofeng Lin
IPC分类号： C12N15/82 , C12N9/02
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

2. 发明授权

US10947555B2 Herbicide resistance genes 有权
公开(公告)号：US10947555B2
公开(公告)日：2021-03-16
申请号：US15288406
申请日：2016-10-07
申请人： Dow AgroSciences LLC
发明人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
IPC分类号： C12N15/82 , C12N9/02
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

3. 发明授权

US08916752B2 Herbicide resistance genes 有权
标题翻译：除草剂抗性基因
公开(公告)号：US08916752B2
公开(公告)日：2014-12-23
申请号：US13647081
申请日：2012-10-08
申请人： Dow AgroSciences LLC
发明人： Terry R. Wright , Justin M. Lira , Terence Anthony Walsh , Donald Merlo , Jayakumar P. Samuel , Gaofeng Lin
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , C12N9/0071 , C12N15/8275
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to pyridyloxyacetate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-12 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
摘要翻译：本发明提供了不仅对2,4-D有抗性的新型植物，而且还提供了对吡啶氧基乙酸酯除草剂的抗性。迄今为止，没有任何期望或建议，通过引入单一基因可以产生具有这两种有利性质的植物。本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。根据本发明使用的优选酶和基因在本文中称为AAD-12（丙烯酰氧基烷酸酯加氧酶）。这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

4. 发明授权

US11149283B2 Herbicide resistance genes 有权
公开(公告)号：US11149283B2
公开(公告)日：2021-10-19
申请号：US17175966
申请日：2021-02-15
申请人： DOW AGROSCIENCES LLC
发明人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
IPC分类号： C12N15/82 , C12N9/02
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

5. 发明申请

US20160304900A1 SYNTHETIC GENES 审中-公开
标题翻译：合成基因
公开(公告)号：US20160304900A1
公开(公告)日：2016-10-20
申请号：US15194925
申请日：2016-06-28
申请人： Dow AgroSciences LLC
发明人： Ignacio M. Larrinua , Donald Merlo , Avutu Sambi Reddy , Arvind Kumar ThirumalaiswamySekhar , Aaron T. Woosley
IPC分类号： C12N15/82 , C07K14/325 , C07K14/37 , C07K14/38 , C07K14/415 , C07K14/435 , C12N9/02 , C07K14/195
CPC分类号： C12N15/8286 , A23D9/00 , A23L7/10 , A23L7/198 , A23L11/00 , A23L11/05 , A23L19/10 , C07K14/195 , C07K14/325 , C07K14/37 , C07K14/38 , C07K14/415 , C07K14/43595 , C11B1/00 , C12N9/0065 , C12N9/0069 , C12N9/0083 , C12Y111/01015 , C12Y113/00 , Y02A40/162
摘要： The invention provides synthetic nucleic acid sequences encoding proteins of interest that are particularly adapted to express well in plants. The claimed synthetic sequences utilize plant-optimized codons roughly in the same frequency at which they are utilized, on average, in genes naturally occurring in the plant species. The invention further includes synthetic DNA sequence for herbicide tolerance, water and/or heat stress tolerance, healthy oil modifications and for transformation marker genes and selectable marker genes are used. DNA construct and transgenic plants containing the synthetic sequences are taught as are methods and compositions for using the plants in agriculture.
摘要翻译：本发明提供编码感兴趣的蛋白质的合成核酸序列，其特别适于在植物中表达良好。所要求的合成序列利用植物优化的密码子，其平均来说在天然存在于植物物种中的基因中使用大致相同的频率。本发明还包括用于除草剂耐受性，水和/或热胁迫耐受性，健康油改性和用于转化标记基因和选择标记基因的合成DNA序列。教导含有合成序列的DNA构建体和转基因植物，以及在农业中使用植物的方法和组合物。

6. 发明授权

US11299745B1 Herbicide resistance genes 有权
公开(公告)号：US11299745B1
公开(公告)日：2022-04-12
申请号：US17403476
申请日：2021-08-16
申请人： DOW AGROSCIENCES LLC
发明人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
IPC分类号： C12N15/82 , C12N9/02
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

7. 发明授权

US10174337B2 Herbicide resistance genes 有权
公开(公告)号：US10174337B2
公开(公告)日：2019-01-08
申请号：US14820893
申请日：2015-08-07
申请人： Dow AgroSciences LLC
发明人： Terry Wright , Justin Lira , Donald Merlo , Nicole Arnold
IPC分类号： C12N15/82 , C12N9/02
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D and other phenoxy auxin herbicides, but also to aryloxyphenoxypropionate herbicides. Heretofore, there was no expectation or suggestion that a plant with both of these advantageous properties could be produced by the introduction of a single gene. The subject invention also includes plants that produce one or more enzymes of the subject invention alone or “stacked” together with another herbicide resistance gene, preferably a glyphosate resistance gene, so as to provide broader and more robust weed control, increased treatment flexibility, and improved herbicide resistance management options. More specifically, preferred enzymes and genes for use according to the subject invention are referred to herein as AAD (aryloxyalkanoate dioxygenase) genes and proteins. No α-ketoglutarate-dependent dioxygenase enzyme has previously been reported to have the ability to degrade herbicides of different chemical classes and modes of action. This highly novel discovery is the basis of significant herbicide tolerant crop trait opportunities as well as development of selectable marker technology. The subject invention also includes related methods of controlling weeds. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the formation of, and controlling, weeds that are resistant (or naturally more tolerant) to one or more herbicides such as glyphosate.

8. 发明授权

US09765350B2 Methods and compositions for recombination a gene-deficient strains of Agrobacterium tumefaciens 有权
公开(公告)号：US09765350B2
公开(公告)日：2017-09-19
申请号：US14844731
申请日：2015-09-03
申请人： Dow AgroSciences LLC
发明人： Manju Gupta , Sara Bennett , Sandeep Kumar , Donald Merlo , Nagesh Sardesai
IPC分类号： C12N15/82 , C12N15/74
CPC分类号： C12N15/8205 , C12N15/743
摘要： The present disclosure provides novel compositions and methods for the production and use of Agrobacterium tumefaciens strains (for example LBA4404) that are deficient in RecA activity relative to the parent strain. Combinations with other gene-deficient-strains of Agrobacterium tumefaciens are also disclosed. Specifically, two exemplary s recA minus strains, UIA777 where chloramphenicol resistant gene disrupting the recA gene and UIA770 where kanamycin resistant gene disrupting the recA gene are provided.

9. 发明申请

US20160002662A1 USES AND DETECTION OF HERBICIDE RESISTANCE GENES FOR RESISTANCE TO ARYLOXYALKANOATE HERBICIDES 有权
标题翻译：使用和检测抗药性杀草剂的抗药性基因
公开(公告)号：US20160002662A1
公开(公告)日：2016-01-07
申请号：US14725328
申请日：2015-05-29
申请人： DOW AGROSCIENCES LLC
发明人： Justin M. Lira , Erika Snodderley , Andrew E. Robinson , Terry R. Wright , Donald Merlo
IPC分类号： C12N15/82
CPC分类号： C12N15/8274 , C12N9/0004 , C12N15/8275
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.
摘要翻译：本发明提供了不仅对2,4-D有抗性的新型植物，而且还涉及吡啶氧基乙酸酯除草剂。本发明还包括与一种或多种其它除草剂抗性基因一起产生本发明一种或多种酶“堆叠”的植物。本发明能够以新的方式使用除草剂的新型组合。此外，本发明提供了防止对一种或多种除草剂如草甘膦具有抗性的杂草的发育和控制菌株的新方法。根据本发明使用的优选酶和基因在本文中称为AAD-13（丙烯酰氧基烷酸酯加氧酶）。这种高度新颖的发现是显着的除草剂耐受性作物性状和选择性标记机会的基础。

10. 发明授权

US09624505B2 Uses and detection of herbicide resistance genes for resistance to aryloxyalkanoate herbicides 有权
公开(公告)号：US09624505B2
公开(公告)日：2017-04-18
申请号：US14725328
申请日：2015-05-29
申请人： DOW AGROSCIENCES LLC
发明人： Justin M. Lira , Erika Snodderley , Andrew E. Robinson , Terry R. Wright , Donald Merlo
IPC分类号： C12N15/82 , C07H21/04 , C12N9/02
CPC分类号： C12N15/8274 , C12N9/0004 , C12N15/8275
摘要： The subject invention provides novel plants that are not only resistant to 2,4-D, but also to a pyridyloxyacetate herbicide. The subject invention also includes plants that produce one or more enzymes of the subject invention “stacked” together with one or more other herbicide resistance genes. The subject invention enables novel combinations of herbicides to be used in new ways. Furthermore, the subject invention provides novel methods of preventing the development of, and controlling, strains of weeds that are resistant to one or more herbicides such as glyphosate. The preferred enzyme and gene for use according to the subject invention are referred to herein as AAD-13 (AryloxyAlkanoate Dioxygenase). This highly novel discovery is the basis of significant herbicide tolerant crop trait and selectable marker opportunities.

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