专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20130130377A1 NOVEL SIRNA STRUCTURE FOR MINIMIZING OFF-TARGET EFFECTS CAUSED BY ANTISENSE STRANDS, AND USE THEREOF 有权
标题翻译：用于最小化抗反射结果引起的非目标效应的新SIRNA结构及其使用
公开(公告)号：US20130130377A1
公开(公告)日：2013-05-23
申请号：US13505965
申请日：2010-11-04
申请人： Dong Ki Lee , Dua Pooja
发明人： Dong Ki Lee , Dua Pooja
IPC分类号： C07H21/02
CPC分类号： C07H21/02 , C12N15/111 , C12N15/113 , C12N2310/14 , C12N2310/533 , C12N2320/53
摘要： The present invention relates to a novel siRNA structure and the use thereof, and more particularly to a double-stranded siRNA molecule comprising an antisense strand and a sense strand, wherein the siRNA molecule has at least one single nucleotide bulge formed by introducing a single nucleotide into the antisense strand, particularly at position 2 from the 5′ end, and to a method of using the same to silence a target gene. The siRNA molecule of the invention shows high target gene silencing efficiency while minimizing off-target effects caused by the antisense strand, and thus has improved target selectivity. Accordingly, the siRNA molecule of the invention can be substituted for conventional siRNA molecules and can be widely be used in siRNA-based gene silencing techniques, including gene therapy.
摘要翻译：本发明涉及新型siRNA结构及其用途，更具体地涉及包含反义链和有义链的双链siRNA分子，其中siRNA分子具有通过引入单个核苷酸形成的至少一个单核苷酸突出进入反义链，特别是在5'末端的2位，以及使用该反义链沉默靶基因的方法。本发明的siRNA分子显示出高的靶基因沉默效率，同时最小化由反义链引起的脱靶效应，从而提高目标选择性。因此，本发明的siRNA分子可以代替常规的siRNA分子，可广泛用于基于siRNA的基因沉默技术，包括基因治疗。

2. 发明申请

US20120142013A1 NUCLEIC ACID APTAMER CAPABLE OF BINDING SPECIFICALLY TO PANCREATIC CANCER CELLS OR TISSUES AND USE THEREOF 有权
标题翻译：具有特异性结合胰腺癌细胞或组织的核酸APTAMER及其用途
公开(公告)号：US20120142013A1
公开(公告)日：2012-06-07
申请号：US13375347
申请日：2010-06-01
申请人： Dong Ki Lee , Dua Pooja
发明人： Dong Ki Lee , Dua Pooja
IPC分类号： C12Q1/68 , C07H21/04 , C07H21/02
CPC分类号： C12N15/115 , A61K48/00 , C12N2310/16 , G01N33/57438
摘要： The present invention relates to a nucleic acid aptamer which can specifically recognize and bind to pancreatic cancer cells or tissues. The nucleic acid aptamer can bind specifically only to pancreatic cancer cells or tissues without binding to normal pancreatic cancer tissue, and thus can be effectively used as a composition for diagnosing and treating pancreatic cancer. In addition, the nucleic acid aptamer can detect not only the terminal pancreatic cancer cell line Capan-1, but also the early pancreatic cancer cell line Panc-1, and thus can be used for early diagnosis of pancreatic cancer, thereby contributing to increasing the survival rate of pancreatic cancer patients.
摘要翻译：本发明涉及可以特异性识别并结合胰腺癌细胞或组织的核酸适体。核酸适体可以仅与胰腺癌细胞或组织结合而不与正常胰腺癌组织结合，因此可以有效地用作诊断和治疗胰腺癌的组合物。此外，核酸适配体不仅可以检测末端胰腺癌细胞系Capan-1，还可以检测早期胰腺癌细胞系Panc-1，从而可以用于胰腺癌的早期诊断，从而有助于增加胰腺癌患者生存率。

3. 发明授权

US09260470B2 SiRNA structure for minimizing off-target effects caused by antisense strands, and use thereof 有权
标题翻译：用于最小化由反义链引起的脱靶效应的SiRNA结构及其用途
公开(公告)号：US09260470B2
公开(公告)日：2016-02-16
申请号：US13505965
申请日：2010-11-04
申请人： Dong Ki Lee , Dua Pooja
发明人： Dong Ki Lee , Dua Pooja
IPC分类号： C07H21/04 , C07H21/02 , C12N15/11 , C12N15/113
CPC分类号： C07H21/02 , C12N15/111 , C12N15/113 , C12N2310/14 , C12N2310/533 , C12N2320/53
摘要： The present invention relates to a novel siRNA structure and the use thereof, and more particularly to a double-stranded siRNA molecule comprising an antisense strand and a sense strand, wherein the siRNA molecule has at least one single nucleotide bulge formed by introducing a single nucleotide into the antisense strand, particularly at position 2 from the 5′ end, and to a method of using the same to silence a target gene. The siRNA molecule of the invention shows high target gene silencing efficiency while minimizing off-target effects caused by the antisense strand, and thus has improved target selectivity. Accordingly, the siRNA molecule of the invention can be substituted for conventional siRNA molecules and can be widely be used in siRNA-based gene silencing techniques, including gene therapy.
摘要翻译：本发明涉及新型siRNA结构及其用途，更具体地涉及包含反义链和有义链的双链siRNA分子，其中siRNA分子具有通过引入单个核苷酸形成的至少一个单核苷酸突出进入反义链，特别是在5'末端的2位，以及使用该反义链沉默靶基因的方法。本发明的siRNA分子显示出高的靶基因沉默效率，同时最小化由反义链引起的脱靶效应，从而提高目标选择性。因此，本发明的siRNA分子可以代替常规的siRNA分子，可广泛用于基于siRNA的基因沉默技术，包括基因治疗。

4. 发明授权

US08563711B2 Nucleic acid aptamer capable of binding specifically to pancreatic cancer cells or tissues and use thereof 有权
标题翻译：能够特异性结合胰腺癌细胞或组织的核酸适体及其用途
公开(公告)号：US08563711B2
公开(公告)日：2013-10-22
申请号：US13375347
申请日：2010-06-01
申请人： Dong Ki Lee , Dua Pooja
发明人： Dong Ki Lee , Dua Pooja
IPC分类号： C07H21/04 , C12Q1/68
CPC分类号： C12N15/115 , A61K48/00 , C12N2310/16 , G01N33/57438
摘要： The present invention relates to a nucleic acid aptamer which can specifically recognize and bind to pancreatic cancer cells or tissues. The nucleic acid aptamer can bind specifically only to pancreatic cancer cells or tissues without binding to normal pancreatic cancer tissue, and thus can be effectively used as a composition for diagnosing and treating pancreatic cancer. In addition, the nucleic acid aptamer can detect not only the terminal pancreatic cancer cell line Capan-1, but also the early pancreatic cancer cell line Panc-1, and thus can be used for early diagnosis of pancreatic cancer, thereby contributing to increasing the survival rate of pancreatic cancer patients.
摘要翻译：本发明涉及可以特异性识别和结合胰腺癌细胞或组织的核酸适体。核酸适体可以仅与胰腺癌细胞或组织结合而不与正常胰腺癌组织结合，因此可以有效地用作诊断和治疗胰腺癌的组合物。此外，核酸适配体不仅可以检测末端胰腺癌细胞系Capan-1，还可以检测早期胰腺癌细胞系Panc-1，从而可以用于胰腺癌的早期诊断，从而有助于增加胰腺癌患者生存率。

5. 发明授权

US09637742B2 Nucleic acid molecules inducing RNA interference, and uses thereof 有权
公开(公告)号：US09637742B2
公开(公告)日：2017-05-02
申请号：US13880670
申请日：2011-09-07
申请人： Dong Ki Lee
发明人： Dong Ki Lee
IPC分类号： C12N15/113 , A61K48/00 , A61P35/00 , A61K31/7088 , C12N15/11 , A61K31/7105 , A61K31/711
CPC分类号： C12N15/1135 , A61K31/7088 , A61K31/7105 , A61K31/711 , C12N15/111 , C12N15/113 , C12N2310/12 , C12N2310/14 , C12N2310/3519 , C12N2310/50
摘要： The present invention relates to an RNAi-inducing nucleic acid molecule having a new structure and the use thereof, and more particularly to a novel nucleic acid molecule having a structure comprising a first strand, which is 24-121 nt in length and comprises a region complementary to a target nucleic acid, and a second strand which is 13-21 nt in length and has a region that binds complementarily to the region of the first strand, which is complementary to the target nucleic acid, so that the nucleic acid molecule inhibits the expression of a target gene with increased efficiency, and to a method of inhibiting the expression of a target gene using the nucleic acid molecule. The nucleic acid molecule structure of the present invention increases the efficiency with which the nucleic acid molecule inhibits the target gene. Alternatively, the nucleic acid molecule of the present invention can either increase the ability of the siRNA to bind to the target gene or cause synergistic cleavage, by introduction of antisense DNA, antisense RNA, ribozyme or DNAzyme, thereby increasing the efficiency with which the nucleic acid molecule inhibits the target gene. In addition, when the nucleic acid molecule according to the present invention is used, the efficiency with which the target gene is inhibited can be maintained for an extended period of time. Accordingly, the RNAi-inducing nucleic acid molecule of the present invention can be effectively used for the treatment of cancer or viral infection in place of conventional siRNA molecules.

6. 发明申请

US20130273657A1 NUCLEIC ACID MOLECULES INDUCING RNA INTERFERENCE, AND USES THEREOF 有权
标题翻译：诱导RNA干扰的核酸分子及其用途
公开(公告)号：US20130273657A1
公开(公告)日：2013-10-17
申请号：US13880670
申请日：2011-09-07
申请人： Dong Ki Lee
发明人： Dong Ki Lee
IPC分类号： C12N15/113
CPC分类号： C12N15/1135 , A61K31/7088 , A61K31/7105 , A61K31/711 , C12N15/111 , C12N15/113 , C12N2310/12 , C12N2310/14 , C12N2310/3519 , C12N2310/50
摘要： The present invention relates to an RNAi-inducing nucleic acid molecule having a new structure and the use thereof, and more particularly to a novel nucleic acid molecule having a structure comprising a first strand, which is 24-121 nt in length and comprises a region complementary to a target nucleic acid, and a second strand which is 13-21 nt in length and has a region that binds complementarily to the region of the first strand, which is complementary to the target nucleic acid, so that the nucleic acid molecule inhibits the expression of a target gene with increased efficiency, and to a method of inhibiting the expression of a target gene using the nucleic acid molecule. The nucleic acid molecule structure of the present invention increases the efficiency with which the nucleic acid molecule inhibits the target gene. Alternatively, the nucleic acid molecule of the present invention can either increase the ability of the siRNA to bind to the target gene or cause synergistic cleavage, by introduction of antisense DNA, antisense RNA, ribozyme or DNAzyme, thereby increasing the efficiency with which the nucleic acid molecule inhibits the target gene. In addition, when the nucleic acid molecule according to the present invention is used, the efficiency with which the target gene is inhibited can be maintained for an extended period of time. Accordingly, the RNAi-inducing nucleic acid molecule of the present invention can be effectively used for the treatment of cancer or viral infection in place of conventional siRNA molecules.
摘要翻译：本发明涉及具有新结构的RNAi诱导核酸分子及其用途，更具体地涉及具有包含长度为24-121nt的第一链的结构的新型核酸分子，其包含区域与靶核酸互补，第二链长度为13-21nt，并且具有与靶核酸互补的与第一链的区域互补结合的区域，使得核酸分子抑制提高效率的靶基因的表达，以及使用该核酸分子抑制靶基因表达的方法。本发明的核酸分子结构提高了核酸分子抑制靶基因的效率。或者，通过引入反义DNA，反义RNA，核酶或DNA酶，本发明的核酸分子可以增加siRNA与靶基因结合的能力或引起协同切割，从而提高核酸的效率酸分子抑制靶基因。此外，当使用根据本发明的核酸分子时，可以长时间保持靶基因被抑制的效率。因此，本发明的RNAi诱导型核酸分子可以有效地用于治疗癌症或病毒感染来代替常规的siRNA分子。

7. 发明申请

US20180127747A1 SIRNA STRUCTURE FOR MINIMIZING OFF-TARGET EFFECTS AND RELAXING SATURATION OF RNAI MACHINERY AND THE USE THEREOF 审中-公开
公开(公告)号：US20180127747A1
公开(公告)日：2018-05-10
申请号：US15678823
申请日：2017-08-16
申请人： Dong Ki Lee , Chan Il Chang
发明人： Dong Ki Lee , Chan Il Chang
IPC分类号： C12N15/11
CPC分类号： C12N15/111 , C12N2310/14 , C12N2320/50
摘要： The present invention relates to a siRNA structure and the use thereof. More particularly, the invention relates to a double-stranded small interfering RNA molecule (siRNA molecule) comprising a 19-21 nucleotide (nt) antisense strand and a 15-19 nt sense strand having a sequence complementary to the antisense sequence, wherein the 5′ end of the antisense strand has a blunt end and the 3′ end of the antisense strand has an overhang, and to a method for silencing the expression of a target gene using the siRNA molecule.

8. 发明申请

US20120168451A1 DRINKING CUP THAT ROTATABLY ATTACHES TO A PLASTIC BOTTLE FOR CLOSURE AND PROTECTION 审中-公开
标题翻译：饮料杯，可旋转连接到塑料瓶上，用于封闭和保护
公开(公告)号：US20120168451A1
公开(公告)日：2012-07-05
申请号：US12980332
申请日：2010-12-29
申请人： Dong Ki Lee
发明人： Dong Ki Lee
IPC分类号： A47G19/22
CPC分类号： A47G19/2205 , B65D41/26
摘要： A drinking cup with a tubular protrusion molded to the bottom and having an internal thread that allows it to be rotatably attached to and detached from a bottle with a small cylindrical neck. The internal thread cooperates with an external thread that is molded on the small cylindrical neck of the bottle so that the drinking cup can function as a cap to close and seal the bottle when it is inverted and placed over the neck of the bottle.
摘要翻译：具有模制到底部并具有内螺纹的管状突起的饮杯，其允许其可旋转地附接到具有小圆柱形颈部的瓶子上并从瓶子上拆下。内螺纹与模制在瓶的小圆柱形颈部上的外螺纹配合，使得饮杯可以用作盖子，以在瓶子倒置并放置在瓶颈上时关闭和密封瓶子。

9. 发明申请

US20120006835A1 DRINKING CUP THAT ATTACHES TO A BEVERAGE CAN FOR CLOSURE AND PROTECTION 审中-公开
标题翻译：饮用水可以连接到饮料上，可以进行封闭和保护
公开(公告)号：US20120006835A1
公开(公告)日：2012-01-12
申请号：US12833983
申请日：2010-07-11
申请人： Dong Ki Lee
发明人： Dong Ki Lee
IPC分类号： B65D90/02
CPC分类号： A47G19/2205 , B65D1/265
摘要： A drinking cup with a gripping mechanism that allows it to attach to a beverage can for closure to minimize the amount of dissolved gases escape from a carbonated beverage and for protection to minimize the amount of beverage spillage when the can is inadvertently dropped or tilted sideways. The drinking cup is designed with thick and thin sections that extend vertically around the outside of the cup to provide sufficient rigidity and flexibility to allow it to operate as a drinking cup and as a cover that snaps on to and off from the top of a beverage can.
摘要翻译：具有夹持机构的饮用杯，其允许其附接到饮料罐以封闭，以使溶解的气体从碳酸饮料中排出的量最小化，并且用于保护当罐被不经意地掉落或侧向倾斜时最小化饮料溢出的量。饮用杯被设计成具有厚而薄的部分，其围绕杯的外部垂直延伸，以提供足够的刚性和柔性，以允许其作为饮用杯和作为从饮料顶部抵接和离开的盖子操作能够。

10. 发明申请

US20170298358A1 TREATMENT OF IDIOPATHIC PULMONARY FIBROSIS USING RNA COMPLEXES THAT TARGET CONNECTIVE TISSUE GROWTH FACTOR 审中-公开
公开(公告)号：US20170298358A1
公开(公告)日：2017-10-19
申请号：US15483305
申请日：2017-04-10
申请人： Dong Ki Lee , Sun Woo Hong , Tae Yeon Lee , Sae Lo Oom Lee , Ji Hyun Kim , Yu Ran Na , Young-Dong Kim
发明人： Dong Ki Lee , Sun Woo Hong , Tae Yeon Lee , Sae Lo Oom Lee , Ji Hyun Kim , Yu Ran Na , Young-Dong Kim
IPC分类号： C12N15/113 , A61K9/00
CPC分类号： C12N15/1136 , A61K9/007 , C12N2310/14 , C12N2310/315 , C12N2310/321 , C12N2310/3515 , C12N2310/50
摘要： In certain aspects, provided herein are RNA complexes (e.g., asymmetric RNA complexes, such as asiRNAs or cell penetrating asiRNAs) that inhibit CTGF expression and are therefore useful for treating idiopathic pulmonary fibrosis.

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式