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    • 3. 发明授权
    • Horizontal slab gel electrophoresis
    • 水平平板凝胶电泳
    • US4234400A
    • 1980-11-18
    • US6826
    • 1979-01-26
    • Donald A. KaplanGary L. Wilcox
    • Donald A. KaplanGary L. Wilcox
    • G01N27/447G01N27/26G01N27/28
    • G01N27/44782G01N27/44756
    • An improved horizontal slab gel electrophoresis apparatus having compartments for vertical wicks at each end of a removable horizontal trough. Adjacent to the vertical wicks are vertical buffer compartments with built in electrodes. A partition separates the vertical wicks from the buffer compartment and includes a slot with a silicone plug which can be removed after curing or hardening of the vertical wicks to permit communication between the buffer and the gel slab. Flow adapters and an interconnecting tube permit constant circulation of the buffer to maintain equal volume and pH. The horizontal trough has slots for insertion of combs to form up to seventy-five sample wells and additional slots at each end for sealing the end of the horizontal slab when the gel slab is removed. The apparatus includes coils in a horizontal bed or plate beneath the trough for cooling the gel slab during a run and levelling screws for levelling the horizontal trough before pouring the gel slab.
    • 一种改进的水平平板凝胶电泳装置,其具有在可移除的水平槽的每一端处用于垂直芯的隔室。 与垂直灯芯相邻的是内置电极的垂直缓冲隔间。 隔板将垂直芯与缓冲隔间分开,并且包括具有硅胶塞的槽,其可以在固化或硬化垂直芯之后被去除以允许缓冲器和凝胶板之间的连通。 流动适配器和互连管允许缓冲液的恒定循环以保持相等的体积和pH。 水平槽具有用于插入梳子以形成多达七十五个样品孔的槽,并且在每个端部具有另外的槽,用于在去除凝胶板时密封水平板的端部。 该设备包括在槽下面的水平床或板中的线圈,用于在运行期间冷却凝胶板,并且在调整螺钉之前,在浇注凝胶板之前调平水平槽。
    • 6. 发明授权
    • Horizontal slab gel electrophoresis
    • 水平平板凝胶电泳
    • US4151065A
    • 1979-04-24
    • US873448
    • 1978-01-30
    • Donald A. KaplanGary L. Wilcox
    • Donald A. KaplanGary L. Wilcox
    • G01N27/447G01N27/26G01N27/28
    • G01N27/44756
    • An improved horizontal slab gel electrophoresis apparatus having compartments for vertical wicks at each end of a removable horizontal trough. Adjacent to the vertical wicks are vertical buffer compartments with built in electrodes. A partition separates the vertical wirks from the buffer compartment and includes a slot with a silicone plug which can be removed after curing or hardening of the vertical wicks to permit communication between the buffer and the gel slab. Flow adapters and an interconnecting tube permit constant circulation of the buffer to maintain equal volume and pH. The horizontal trough has slots for insertion of combs to form up to seventy-five sample wells and additional slots at each end for sealing the end of the horizontal slab when the gel slab is removed. The apparatus includes coils in a horizontal bed or plate beneath the trough for cooling the gel slab during a run and levelling screws for levelling the horizontal trough before pouring the gel slab.
    • 一种改进的水平平板凝胶电泳装置,其具有在可移除的水平槽的每一端处用于垂直芯的隔室。 与垂直灯芯相邻的是内置电极的垂直缓冲隔间。 隔板将垂直织带与缓冲隔间分开,并且包括具有硅胶塞的槽,其可以在固化或硬化垂直芯之后被去除,以允许缓冲器和凝胶板之间的连通。 流动适配器和互连管允许缓冲液的恒定循环以保持相等的体积和pH。 水平槽具有用于插入梳子以形成多达七十五个样品孔的槽,并且在每个端部具有另外的槽,用于在去除凝胶板时密封水平板的端部。 该设备包括在槽下面的水平床或板中的线圈,用于在运行期间冷却凝胶板,并且在调整螺钉之前,在浇注凝胶板之前调平水平槽。