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1. 发明申请

US20060191831A1 NANOPUMP DEVICES AND METHODS 有权
公开(公告)号：US20060191831A1
公开(公告)日：2006-08-31
申请号：US11279515
申请日：2006-04-12
申请人： Derek Hansford , Robbie Walczak , Anthony Boiarski
发明人： Derek Hansford , Robbie Walczak , Anthony Boiarski
IPC分类号： B01D63/00
CPC分类号： F04B19/006 , B01D57/02 , B01D61/56 , B01L3/5027 , F04B17/00
摘要： Disclosed is a nanopump for pumping small volumes of electrolyte solution under the control of a voltage source. The device includes a chamber and a nanopore membrane which partitions the chamber into upstream and downstream regions. When a voltage potential is applied across the membrane, electroosmotic flow through the membrane channels produces a precise-volume flow between the two chamber regions. Also disclosed is a method for precis-volume pumping employing the nanopump. Also disclosed is device for determining the lengths of nucleic acid fragments in an electrolyte solution of different-length fragments is disclosed. The device includes a chamber having disposed therein, a nanopore channel extending between upstream and downstream chamber regions. By applying a voltage potential across upstream and downstream electrodes in the chamber regions, individual nucleic acid fragments contained in the solution are moved through the channel. A current detector detects time-dependent current flow across said membrane, and from the measured flow times, fragments lengths can be estimated. Also disclosed is a device for separating macromolecules in a solution of macromolecules having different molecular sizes. The device includes a separation chamber having upstream and downstream ends, and one or more nanopore membranes disposed in the chamber between said upstream and downstream ends, partitioning the chamber into two or more chamber regions, respectively. Upstream and downstream electrodes are disposed at the upstream and downstream ends of the chamber, respectively. A controller in the device has a power source operatively connected to the electrodes for applying a selected voltage potential across said channel, to pump solution through each of said membranes, in an upstream-to-downstream direction, wherein macromolecules contained in the solution are filtered at each successive membrane, to concentrate successively smaller macromolecules in successively more downstream chamber regions.

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