专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US08604181B2 Promoter variants for expressing genes in a fungal cell 有权
标题翻译：用于在真菌细胞中表达基因的启动子变体
公开(公告)号：US08604181B2
公开(公告)日：2013-12-10
申请号：US13424018
申请日：2012-03-19
申请人： Debbie Yaver , Peter Nham
发明人： Debbie Yaver , Peter Nham
IPC分类号： C07H21/04 , C12N15/63 , C12N15/00
CPC分类号： C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/58 , C12N9/93 , C12N15/113 , C12P1/02 , C12P21/00 , C12Y107/01003 , C12Y301/01003 , C12Y304/21004 , C12Y603/04002
摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.
摘要翻译：本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列与包含选自SEQ ID NO：2,3,4,5,6,7,8,9,10,11和12的启动子变体的第二核酸序列连接; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

2. 发明申请

US20080274500A1 Promoter variants for expressing genes in a fungal cell 有权
标题翻译：用于在真菌细胞中表达基因的启动子变体
公开(公告)号：US20080274500A1
公开(公告)日：2008-11-06
申请号：US12054125
申请日：2008-03-24
申请人： Debbie Yaver , Peter Nham
发明人： Debbie Yaver , Peter Nham
IPC分类号： C12P21/06 , C07H21/04 , C12N15/00 , C12N1/15
CPC分类号： C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/58 , C12N9/93 , C12N15/113 , C12P1/02 , C12P21/00 , C12Y107/01003 , C12Y301/01003 , C12Y304/21004 , C12Y603/04002
摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.
摘要翻译：本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

3. 发明申请

US20120190067A1 Promoter Variants For Expressing Genes In A Fungal Cell 有权
标题翻译：在真菌细胞中表达基因的启动子变体
公开(公告)号：US20120190067A1
公开(公告)日：2012-07-26
申请号：US13424018
申请日：2012-03-19
申请人： Debbie Yaver , Peter Nham
发明人： Debbie Yaver , Peter Nham
IPC分类号： C12P21/00 , C12N1/19 , C12N15/63 , C12N1/15 , C12N9/00 , C12N15/113
CPC分类号： C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/58 , C12N9/93 , C12N15/113 , C12P1/02 , C12P21/00 , C12Y107/01003 , C12Y301/01003 , C12Y304/21004 , C12Y603/04002
摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NOs: 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, and 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.
摘要翻译：本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列与包含选自SEQ ID NO：2,3,4,5,6,7,8,9,10,11和12的启动子变体的第二核酸序列连接; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

4. 发明授权

US08138325B2 Promoter variants for expressing genes in a fungal cell 有权
标题翻译：用于在真菌细胞中表达基因的启动子变体
公开(公告)号：US08138325B2
公开(公告)日：2012-03-20
申请号：US12054125
申请日：2008-03-24
申请人： Debbie Yaver , Peter Nham
发明人： Debbie Yaver , Peter Nham
IPC分类号： C07H21/04 , C12P21/00 , C12N1/15
CPC分类号： C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/58 , C12N9/93 , C12N15/113 , C12P1/02 , C12P21/00 , C12Y107/01003 , C12Y301/01003 , C12Y304/21004 , C12Y603/04002
摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.
摘要翻译：本发明涉及生物体的制造方法，其特征在于，包括：（a）在有助于生物生物的培养基中培养真菌宿主细胞，其中所述真菌宿主细胞可操作地包含编码生物物质的第一核酸序列连接到第二核酸序列，其包含选自SEQ ID NO：2，SEQ ID NO：3，SEQ ID NO：4，SEQ ID NO：5，SEQ ID NO：6，SEQ ID NO：：7，SEQ ID NO：8，SEQ ID NO：9，SEQ ID NO：10，SEQ ID NO：11和SEQ ID NO：12; 及其后果; 及其杂交和串联启动子; 和（b）从培养基中分离生物物质。本发明还涉及分离的启动子变体和构建体，载体和真菌宿主细胞，其包含可操作地连接于编码生物物质的核酸序列的启动子变体。

5. 发明授权

US07368262B2 Promoter variants for expressing genes in a fungal cell 有权
公开(公告)号：US07368262B2
公开(公告)日：2008-05-06
申请号：US10716793
申请日：2003-11-18
申请人： Debbie Yaver , Peter Nham
发明人： Debbie Yaver , Peter Nham
IPC分类号： C12N15/00 , C12N1/00 , C12N15/63 , C12Q1/68 , C12N1/20
CPC分类号： C12N15/80 , C12N9/0044 , C12N9/20 , C12N9/58 , C12N9/93 , C12N15/113 , C12P1/02 , C12P21/00 , C12Y107/01003 , C12Y301/01003 , C12Y304/21004 , C12Y603/04002
摘要： The present invention relates to methods for producing a biological substance, comprising: (a) cultivating a fungal host cell in a medium conducive for the production of the biological substance, wherein the fungal host cell comprises a first nucleic acid sequence encoding the biological substance operably linked to a second nucleic acid sequence comprising a promoter variant selected from the group consisting of SEQ ID NO: 2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO: 5, SEQ ID NO: 6, SEQ ID NO: 7, SEQ ID NO: 8, SEQ ID NO: 9, SEQ ID NO: 10, SEQ ID NO: 11, and SEQ ID NO: 12; and a subsequence thereof; and hybrid and tandem promoters thereof; and (b) isolating the biological substance from the cultivation medium. The present invention also relates to the isolated promoter variants and to constructs, vectors, and fungal host cells comprising the promoter variants operably linked to nucleic acid sequences encoding biological substances.

6. 发明授权

US09284588B2 Promoters for expressing genes in a fungal cell 有权
标题翻译：在真菌细胞中表达基因的启动子
公开(公告)号：US09284588B2
公开(公告)日：2016-03-15
申请号：US13885145
申请日：2011-12-15
申请人： Sandra Merino , Debbie Yaver
发明人： Sandra Merino , Debbie Yaver
IPC分类号： C12P21/00 , C12N9/08 , C12N15/80
CPC分类号： C12N15/80 , C12N9/0065 , C12P21/00
摘要： The present invention relates to isolated promoters and constructs, vectors, and fungal host cells comprising such promoters operably linked to polynucleotides encoding polypeptides. The present invention also relates to methods for producing such polypeptides.
摘要翻译：本发明涉及分离的启动子和构建体，载体和包含与编码多肽的多核苷酸可操作地连接的此类启动子的真菌宿主细胞。本发明还涉及生产这种多肽的方法。

7. 发明申请

US20140212977A1 Methods For Producing Multiple Recombinant Polypeptides In A Filamentous Fungal Host Cell 有权
标题翻译：在丝状真菌宿主细胞中产生多种重组多肽的方法
公开(公告)号：US20140212977A1
公开(公告)日：2014-07-31
申请号：US14238691
申请日：2012-08-23
申请人： Debbie Yaver , Qiming Jin
发明人： Debbie Yaver , Qiming Jin
IPC分类号： C12N15/87 , C12N9/42 , C12N15/80
CPC分类号： C12N9/2437 , C12N9/2402 , C12N15/80 , C12N15/87 , C12Y302/01091 , C12Y302/0115
摘要： The present invention relates to methods for constructing a filamentous fungal strain for production of multiple recombinant polypeptides having biological activity. The present invention also relates to methods for producing multiple recombinant polypeptides having biological activity in a filamentous fungal strain. The present invention also relates to filamentous fungal strains expressing multiple recombinant polypeptides having biological activity.
摘要翻译：本发明涉及构建用于生产具有生物活性的多重重组多肽的丝状真菌菌株的方法。本发明还涉及在丝状真菌菌株中生产具有生物活性的多重重组多肽的方法。本发明还涉及表达具有生物活性的多种重组多肽的丝状真菌菌株。

8. 发明授权

US08741611B2 Methods for improving malic acid production in filamentous fungi 失效
公开(公告)号：US08741611B2
公开(公告)日：2014-06-03
申请号：US12870523
申请日：2010-08-27
申请人： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
发明人： Stephen Brown , Sheryl Luttringer , Debbie Yaver , Alan Berry
IPC分类号： C12P7/46 , C12N1/00
CPC分类号： C12P7/46 , C07K14/38 , C12N15/80
摘要： The present invention relates to methods of producing a C4 dicarboxylic acid, comprising: (a) cultivating a filamentous fungal host cell comprising a polynucleotide selected from the group consisting of a heterologous first polynucleotide encoding a C4 dicarboxylic acid transporter, a heterologous second polynucleotide encoding a malate dehydrogenase, and a heterologous third polynucleotide encoding a pyruvate carboxylase; wherein the filamentous fungal host cell is capable of secreting increased levels of the C4 dicarboxylic acid compared to the filamentous fungal host cell without the heterologous polynucleotide when cultivated under the same conditions; and (b) recovering the C4 dicarboxylic acid. The present invention also relates to methods for increasing C4 dicarboxylic acid production, filamentous fungal host cells and malate dehydrogenase variants.

9. 发明申请

US20120192316A1 POLYPEPTIDES HAVING LIPASE ACTIVITY AND POLYNUCLEOTIDES ENCODING SAME 有权
公开(公告)号：US20120192316A1
公开(公告)日：2012-07-26
申请号：US13440626
申请日：2012-04-05
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： A01H5/00 , C07H21/04 , C12N15/63 , C12N9/20 , C12N5/10
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.

10. 发明授权

US07955830B2 Polypeptides having lipase activity and polynucleotides encoding same 有权
标题翻译：具有脂肪酶活性的多肽和编码该多肽的多核苷酸
公开(公告)号：US07955830B2
公开(公告)日：2011-06-07
申请号：US12944557
申请日：2010-11-11
申请人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
发明人： Debbie Yaver , Suzanne Otani , Janine Lin , Christopher Amolo , Kim Borch , Shamkant Anant Patkar , Michael Lamsa , Barbara Cherry
IPC分类号： C11D3/386 , C07K1/00 , C12N9/10
CPC分类号： C12N9/20
摘要： The present invention relates to isolated polypeptides having lipase activity and isolated polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods for producing and using the polypeptides.
摘要翻译：本发明涉及具有脂肪酶活性的分离的多肽和编码多肽的分离的多核苷酸。本发明还涉及包含多核苷酸的核酸构建体，载体和宿主细胞以及生产和使用多肽的方法。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式