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1. 发明授权

US08519099B2 Methods for production of proteins 有权
标题翻译：生产蛋白质的方法
公开(公告)号：US08519099B2
公开(公告)日：2013-08-27
申请号：US13006354
申请日：2011-01-13
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

2. 发明申请

US20070190606A1 Methods for Production of Proteins 有权
标题翻译：蛋白质生产方法
公开(公告)号：US20070190606A1
公开(公告)日：2007-08-16
申请号：US11618723
申请日：2006-12-29
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C12N9/02 , C07H21/04 , C12N1/21 , C12N15/74
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子梯度的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子量梯。

3. 发明申请

US20070178489A1 Compositions and Methods for Enhanced Sensitivity and Specificity of Nucleic Acid Synthesis 审中-公开
标题翻译：增强的核酸合成灵敏度和特异性的组合物和方法
公开(公告)号：US20070178489A1
公开(公告)日：2007-08-02
申请号：US11539347
申请日：2006-10-06
申请人： Mekbib Astatke , Deb Chatterjee , Harini Shadilya
发明人： Mekbib Astatke , Deb Chatterjee , Harini Shadilya
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12Y207/07007
摘要： The present invention relates to polypeptides, compositions and methods for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses polypeptides having affinity for double-stranded and/or single-stranded nucleic acid molecules and/or single-stranded/double-stranded nucleic acid complexes (e.g., primer/template complexes, double-stranded templates, single-stranded templates or single-stranded primers) for use in such enhanced synthesis and more particularly to polymerases having reduced polymerase and optionally reduced exonuclease activities (3′ to 5′ and/or 5′ to 3′ exonuclease activity), and to nucleases having reduced nuclease activity. The polypeptides of the invention are capable of inhibiting nonspecific nucleic acid synthesis at ambient temperature. Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents non-specific nucleic acid synthesis at low temperatures, for example during reaction set up. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the polypeptides or compositions of the invention. The invention also relates to compositions prepared for carrying out the methods of the invention and to compositions made after or during such methods. The invention also generally relates to polypeptides and compositions useful for inhibiting or preventing degradation of various nucleic acid molecules.
摘要翻译：本发明涉及用于增强核酸分子合成的多肽，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体地，本发明公开了对双链和/或单链核酸分子和/或单链/双链核酸复合物（例如，引物/模板复合物，双链模板，单链/ 双链模板或单链引物），更具体地涉及具有降低的聚合酶和任选地减少的核酸外切酶活性（3'至5'和/或5'至3'外切核酸酶活性）的聚合酶，以及具有降低的核酸酶核酸酶活性。本发明的多肽能够在环境温度下抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止在低温下的非特异性核酸合成，例如在反应设置期间。本发明还涉及用于合成，扩增，逆转录或测序包含一种或多种本发明多肽或组合物的核酸分子的试剂盒。本发明还涉及制备用于实施本发明方法的组合物以及在这些方法之后或期间制备的组合物。本发明还通常涉及可用于抑制或预防各种核酸分子降解的多肽和组合物。

4. 发明申请

US20050089922A1 Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis 审中-公开
标题翻译：增强核酸合成灵敏度和特异性的组合物和方法
公开(公告)号：US20050089922A1
公开(公告)日：2005-04-28
申请号：US11007184
申请日：2004-12-09
申请人： Mekbib Astatke , Deb Chatterjee , Gary Gerard
发明人： Mekbib Astatke , Deb Chatterjee , Gary Gerard
IPC分类号： A61K48/00 , C12N9/22 , C12P19/34 , C12Q1/68 , G01N33/53
CPC分类号： C12N15/11 , A61K31/7052 , C12N9/1247 , C12N9/1252 , C12N9/1276 , C12N2310/122 , C12N2310/315 , C12P19/34 , C12Q1/6844 , C12Q1/6869 , C12Y207/07006 , C12Y207/07007 , C12Y207/07049 , C12Q2527/125
摘要： The present invention relates to nucleic acid inhibitors, compositions and method for enhancing synthesis of nucleic acid molecules. In a preferred aspect, the invention relates to inhibition or control of nucleic acid synthesis, sequencing or amplification. Specifically, the present invention discloses nucleic acids having affinity for polypeptides with polymerase activity for use in such synthesis, amplification or sequencing reactions. The nucleic acid inhibitors are capable of inhibiting nonspecific nucleic acid synthesis under certain conditions (e.g., at ambient temperatures). Thus, in a preferred aspect, the invention relates to “hot start” synthesis of nucleic acid molecules. Accordingly, the invention prevents, reduces or substantially reduces nonspecific nucleic acid synthesis. The invention also relates to kits for synthesizing, amplifying, reverse transcribing or sequencing nucleic acid molecules comprising one or more of the nucleic acid inhibitors or compositions of the invention. The invention also relates to using the inhibitors of the invention to prevent viral replication or treat viral infections in a subject. Thus, the invention relates to therapeutic methods and pharmaceutical compositions using the inhibitors of the invention. The invention thus may be used for in vivo and in vitro inhibition of nucleic acid synthesis and/or inhibition of polymerase activity.
摘要翻译：本发明涉及用于增强核酸分子合成的核酸抑制剂，组合物和方法。在优选的方面，本发明涉及抑制或控制核酸合成，测序或扩增。具体而言，本发明公开了对于在这种合成，扩增或测序反应中使用具有聚合酶活性的多肽具有亲和力的核酸。核酸抑制剂能够在某些条件下（例如在环境温度下）抑制非特异性核酸合成。因此，在优选的方面，本发明涉及核酸分子的“热启动”合成。因此，本发明防止，减少或显着降低非特异性核酸合成。本发明还涉及用于合成，扩增，逆转录或测序核酸分子的试剂盒，所述核酸分子包含一种或多种本发明的核酸抑制剂或组合物。本发明还涉及使用本发明的抑制剂来预防病毒复制或治疗受试者的病毒感染。因此，本发明涉及使用本发明的抑制剂的治疗方法和药物组合物。因此，本发明可用于体内和体外抑制核酸合成和/或抑制聚合酶活性。

5. 发明授权

US06703484B2 Methods for production of proteins 失效
公开(公告)号：US06703484B2
公开(公告)日：2004-03-09
申请号：US09004068
申请日：1998-01-08
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K100
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

6. 发明申请

US20110189159A1 METHODS AND COMPOSITIONS FOR PROTEIN DELIVERY 有权
标题翻译：蛋白质递送的方法和组合物
公开(公告)号：US20110189159A1
公开(公告)日：2011-08-04
申请号：US13122513
申请日：2009-10-02
申请人： Deb Chatterjee , Stanislaw Jan Kaczmarcyk
发明人： Deb Chatterjee , Stanislaw Jan Kaczmarcyk
IPC分类号： A61K38/48 , C12N9/48 , C07H21/00 , C12P21/00 , C12N5/10 , C12N15/63 , C07K14/00 , C07K7/06 , A61P35/00
CPC分类号： C07K14/005 , A61K47/6901 , C07K14/00 , C07K14/245 , C07K2319/09 , C07K2319/10 , C07K2319/50 , C07K2319/705 , C07K2319/735 , C12N7/00 , C12N2740/13043 , C12N2760/20222 , C12N2760/20223 , C12N2810/6072 , C12N2810/6081
摘要： The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
摘要翻译：本发明提供蛋白质递送的方法和组合物。本发明具有病毒样颗粒，制备病毒样颗粒的方法和使用病毒样颗粒将蛋白质递送至细胞的方法，以提供蛋白质治疗和治疗疾病或病症。本发明还涉及将蛋白质靶向细胞的方法，蛋白质治疗方法以及使用从全长TUS鉴定的TUS蛋白，NLS或NES来治疗疾病或病症的方法。

7. 发明申请

US20070087356A1 In situ assembling of protein microarrays 有权
标题翻译：蛋白质微阵列的原位组装
公开(公告)号：US20070087356A1
公开(公告)日：2007-04-19
申请号：US11252735
申请日：2005-10-19
申请人： Deb Chatterjee , Kalavathy Sitaraman , James Hartley , Cassio Baptista , David Munroe
发明人： Deb Chatterjee , Kalavathy Sitaraman , James Hartley , Cassio Baptista , David Munroe
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6837 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00725 , G01N33/6845
摘要： The invention provides a microarray and methods for producing a protein microarray. The array comprises multiple nucleic acid molecules immobilized on a substrate, each comprising (i) a protein-binding domain and (ii) a nucleic acid sequence encoding a fusion protein comprising a polypeptide of interest and a DNA-binding protein that binds the protein-binding domain, and one or more fusion proteins produced from the multiple nucleic acid molecules. Each fusion protein is immobilized on the substrate via binding to a nucleic acid sequence comprising the protein-binding domain present on the nucleic acid molecule from which the fusion protein is produced or on the substrate. The invention also provides a method of analyzing protein interactions with, for example, other proteins, lipids and drugs.
摘要翻译：本发明提供了用于产生蛋白质微阵列的微阵列和方法。该阵列包含固定在底物上的多个核酸分子，每个核酸分子包含（i）蛋白质结合结构域和（ii）编码融合蛋白的核酸序列，所述融合蛋白包含目的多肽和结合蛋白质结合蛋白的DNA结合蛋白，结合结构域和由多个核酸分子产生的一种或多种融合蛋白。每个融合蛋白通过与包含存在于产生融合蛋白的核酸分子上存在的蛋白质结合结构域或底物上的核酸序列结合而固定在底物上。本发明还提供了分析与例如其它蛋白质，脂质和药物的蛋白质相互作用的方法。

8. 发明申请

US20060286633A1 Methods for production of proteins 有权
公开(公告)号：US20060286633A1
公开(公告)日：2006-12-21
申请号：US11329401
申请日：2006-01-11
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C07K14/47 , C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

9. 发明申请

US20060269992A1 Methods of production of proteins 有权
标题翻译：蛋白质生产方法
公开(公告)号：US20060269992A1
公开(公告)日：2006-11-30
申请号：US11329418
申请日：2006-01-11
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C07K14/47
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

10. 发明申请

US20050112637A1 Mutant DNA polymerases and uses thereof 审中-公开
标题翻译：突变DNA聚合酶及其用途
公开(公告)号：US20050112637A1
公开(公告)日：2005-05-26
申请号：US10947352
申请日：2004-09-23
申请人： Deb Chatterjee
发明人： Deb Chatterjee
IPC分类号： C12N9/12 , C12Q1/68 , C07H21/04 , C12N1/21 , C12N9/22 , C12N15/74
CPC分类号： C12N9/1252
摘要： The present invention relates to mutant DNA polymerases which incorporate dideoxynucleotides with about the same efficiency as deoxynucleotides. The present invention also related to mutant DNA polymerases which also have substantially reduced 5′-to-3′ exonuclease activity or 3′-to-5′ exonuclease activity. The invention also relates to DNA molecules coding for the mutant DNA polymerases, and hosts containing the DNA molecules.
摘要翻译：本发明涉及掺入与脱氧核苷酸大致相同效力的双脱氧核苷酸的突变型DNA聚合酶。本发明还涉及也具有基本上降低的5'至3'核酸外切酶活性或3'至5'核酸外切酶活性的突变型DNA聚合酶。本发明还涉及编码突变型DNA聚合酶的DNA分子和含有DNA分子的宿主。

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