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1. 发明授权

US08519099B2 Methods for production of proteins 有权
标题翻译：生产蛋白质的方法
公开(公告)号：US08519099B2
公开(公告)日：2013-08-27
申请号：US13006354
申请日：2011-01-13
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

2. 发明申请

US20110189159A1 METHODS AND COMPOSITIONS FOR PROTEIN DELIVERY 有权
标题翻译：蛋白质递送的方法和组合物
公开(公告)号：US20110189159A1
公开(公告)日：2011-08-04
申请号：US13122513
申请日：2009-10-02
申请人： Deb Chatterjee , Stanislaw Jan Kaczmarcyk
发明人： Deb Chatterjee , Stanislaw Jan Kaczmarcyk
IPC分类号： A61K38/48 , C12N9/48 , C07H21/00 , C12P21/00 , C12N5/10 , C12N15/63 , C07K14/00 , C07K7/06 , A61P35/00
CPC分类号： C07K14/005 , A61K47/6901 , C07K14/00 , C07K14/245 , C07K2319/09 , C07K2319/10 , C07K2319/50 , C07K2319/705 , C07K2319/735 , C12N7/00 , C12N2740/13043 , C12N2760/20222 , C12N2760/20223 , C12N2810/6072 , C12N2810/6081
摘要： The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
摘要翻译：本发明提供蛋白质递送的方法和组合物。本发明具有病毒样颗粒，制备病毒样颗粒的方法和使用病毒样颗粒将蛋白质递送至细胞的方法，以提供蛋白质治疗和治疗疾病或病症。本发明还涉及将蛋白质靶向细胞的方法，蛋白质治疗方法以及使用从全长TUS鉴定的TUS蛋白，NLS或NES来治疗疾病或病症的方法。

3. 发明申请

US20070087356A1 In situ assembling of protein microarrays 有权
标题翻译：蛋白质微阵列的原位组装
公开(公告)号：US20070087356A1
公开(公告)日：2007-04-19
申请号：US11252735
申请日：2005-10-19
申请人： Deb Chatterjee , Kalavathy Sitaraman , James Hartley , Cassio Baptista , David Munroe
发明人： Deb Chatterjee , Kalavathy Sitaraman , James Hartley , Cassio Baptista , David Munroe
IPC分类号： C12Q1/68 , C12M1/34
CPC分类号： C12Q1/6837 , B01J2219/00605 , B01J2219/0061 , B01J2219/00612 , B01J2219/00637 , B01J2219/00659 , B01J2219/00725 , G01N33/6845
摘要： The invention provides a microarray and methods for producing a protein microarray. The array comprises multiple nucleic acid molecules immobilized on a substrate, each comprising (i) a protein-binding domain and (ii) a nucleic acid sequence encoding a fusion protein comprising a polypeptide of interest and a DNA-binding protein that binds the protein-binding domain, and one or more fusion proteins produced from the multiple nucleic acid molecules. Each fusion protein is immobilized on the substrate via binding to a nucleic acid sequence comprising the protein-binding domain present on the nucleic acid molecule from which the fusion protein is produced or on the substrate. The invention also provides a method of analyzing protein interactions with, for example, other proteins, lipids and drugs.
摘要翻译：本发明提供了用于产生蛋白质微阵列的微阵列和方法。该阵列包含固定在底物上的多个核酸分子，每个核酸分子包含（i）蛋白质结合结构域和（ii）编码融合蛋白的核酸序列，所述融合蛋白包含目的多肽和结合蛋白质结合蛋白的DNA结合蛋白，结合结构域和由多个核酸分子产生的一种或多种融合蛋白。每个融合蛋白通过与包含存在于产生融合蛋白的核酸分子上存在的蛋白质结合结构域或底物上的核酸序列结合而固定在底物上。本发明还提供了分析与例如其它蛋白质，脂质和药物的蛋白质相互作用的方法。

4. 发明申请

US20060286633A1 Methods for production of proteins 有权
公开(公告)号：US20060286633A1
公开(公告)日：2006-12-21
申请号：US11329401
申请日：2006-01-11
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C07K14/47 , C07K1/00
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.

5. 发明申请

US20060269992A1 Methods of production of proteins 有权
标题翻译：蛋白质生产方法
公开(公告)号：US20060269992A1
公开(公告)日：2006-11-30
申请号：US11329418
申请日：2006-01-11
申请人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
发明人： Deb Chatterjee , Mary Longo , Elizabeth Flynn , Robert Oberfelder
IPC分类号： C12P21/06 , C07K14/47
CPC分类号： C07K1/26 , C07K1/1077 , C07K1/13 , C07K14/001 , C07K2319/00 , C07K2319/01 , C12N9/0036 , C12P21/02 , Y02P20/52
摘要： The current invention provides methods for producing a polypeptide as inclusion bodies in bacterial host cells. The present methods are carried out by forming a gene construct comprising the genetic sequence encoding a polypeptide operatively linked to that of an inclusion partner protein, such as E. coli thioredoxin or a modified E. coli thioredoxin, such that host cells comprising the gene construct produce the polypeptide as intracellular inclusion bodies. The methods of the present invention facilitate the rapid isolation and purification of recombinant proteins. In addition, the present methods may be useful for producing polypeptides or proteins which are small and are typically difficult to express, as well as those proteins that are toxic to host cells such as E. coli. The present invention also provides plasmids, vectors and host cells to be used in the present invention for production of polypeptides, and methods of production of polypeptides using these vectors and host cells. The invention further provides methods for producing protein molecular weight ladders for use in protein gel electrophoresis, as well as proteins and protein molecular weight ladders produced by these methods.
摘要翻译：本发明提供了在细菌宿主细胞中产生作为包涵体的多肽的方法。本发明的方法通过形成包含编码与包含配对蛋白例如大肠杆菌硫氧还蛋白或修饰的大肠杆菌硫氧还蛋白的内含子配偶体蛋白可操作地连接的多肽的遗传序列来进行，使得包含该基因构建体的宿主细胞产生多肽作为细胞内包涵体。本发明的方法促进重组蛋白的快速分离和纯化。此外，本发明的方法可用于生产小而通常难以表达的多肽或蛋白质，以及对宿主细胞如大肠杆菌毒性的那些蛋白质。本发明还提供了用于本发明中用于产生多肽的质粒，载体和宿主细胞，以及使用这些载体和宿主细胞产生多肽的方法。本发明进一步提供了用于蛋白质凝胶电泳的蛋白质分子级梯子的制备方法，以及通过这些方法制备的蛋白质和蛋白质分子级梯子。

6. 发明申请

US20050112637A1 Mutant DNA polymerases and uses thereof 审中-公开
标题翻译：突变DNA聚合酶及其用途
公开(公告)号：US20050112637A1
公开(公告)日：2005-05-26
申请号：US10947352
申请日：2004-09-23
申请人： Deb Chatterjee
发明人： Deb Chatterjee
IPC分类号： C12N9/12 , C12Q1/68 , C07H21/04 , C12N1/21 , C12N9/22 , C12N15/74
CPC分类号： C12N9/1252
摘要： The present invention relates to mutant DNA polymerases which incorporate dideoxynucleotides with about the same efficiency as deoxynucleotides. The present invention also related to mutant DNA polymerases which also have substantially reduced 5′-to-3′ exonuclease activity or 3′-to-5′ exonuclease activity. The invention also relates to DNA molecules coding for the mutant DNA polymerases, and hosts containing the DNA molecules.
摘要翻译：本发明涉及掺入与脱氧核苷酸大致相同效力的双脱氧核苷酸的突变型DNA聚合酶。本发明还涉及也具有基本上降低的5'至3'核酸外切酶活性或3'至5'核酸外切酶活性的突变型DNA聚合酶。本发明还涉及编码突变型DNA聚合酶的DNA分子和含有DNA分子的宿主。

7. 发明授权

US09296790B2 Methods and compositions for protein delivery 有权
标题翻译：用于蛋白质递送的方法和组合物
公开(公告)号：US09296790B2
公开(公告)日：2016-03-29
申请号：US13122513
申请日：2009-10-02
申请人： Deb Chatterjee , Stanislaw Jan Kaczmarcyk
发明人： Deb Chatterjee , Stanislaw Jan Kaczmarcyk
IPC分类号： C12N7/00 , C07K14/005 , A61K47/48 , C07K14/00 , C07K14/245
CPC分类号： C07K14/005 , A61K47/6901 , C07K14/00 , C07K14/245 , C07K2319/09 , C07K2319/10 , C07K2319/50 , C07K2319/705 , C07K2319/735 , C12N7/00 , C12N2740/13043 , C12N2760/20222 , C12N2760/20223 , C12N2810/6072 , C12N2810/6081
摘要： The present invention provides methods and compositions for protein delivery. The invention features virus like particles, methods of making virus like particles and methods of using virus like particles to deliver proteins to a cell, to provide protein therapy and to treat diseases or disorders. The invention also features methods of targeting a protein to a cell, methods of protein therapy and methods of treating diseases or disorders using a TUS protein, a NLS or NES identified from full length TUS.
摘要翻译：本发明提供蛋白质递送的方法和组合物。本发明具有病毒样颗粒，制备病毒样颗粒的方法和使用病毒样颗粒将蛋白质递送至细胞的方法，以提供蛋白质治疗和治疗疾病或病症。本发明还涉及将蛋白质靶向细胞的方法，蛋白质治疗方法以及使用从全长TUS鉴定的TUS蛋白，NLS或NES来治疗疾病或病症的方法。

8. 发明申请

US20070269878A1 COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTION OF NUCLEIC ACID MOLECULES 审中-公开
标题翻译：反向转录核酸分子的组合物和方法
公开(公告)号：US20070269878A1
公开(公告)日：2007-11-22
申请号：US11690715
申请日：2007-03-23
申请人： Gary Gerard , Michael Smith , Deb Chatterjee
发明人： Gary Gerard , Michael Smith , Deb Chatterjee
IPC分类号： C12N9/00
CPC分类号： C12N15/1096 , C12N9/1276 , C12N2740/10011 , C12N2740/11011 , C12Q1/6848 , C12Q2527/101 , C12Q2521/301 , C12Q2521/107
摘要： The present invention is generally related to compositions and methods for the reverse transcription of nucleic acid molecules, especially messenger RNA molecules. Specifically, the invention relates to compositions comprising mixtures of polypeptides having reverse transcriptase (RT) activity, and to methods of producing, amplifying or sequencing nucleic acid molecules (particularly cDNA molecules) using these compositions or polypeptides, particularly at temperatures above about 55° C. The invention also relates to nucleic acid molecules produced by these methods, to vectors and host cells comprising these nucleic acid molecules, and to the use of such nucleic acid molecules to produce desired polypeptides. The invention also relates to methods for producing Rous Sarcoma Virus (RSV) and Avian Myeloblastosis Virus (AMV) RTs or other Avian Sarcoma-Leukosis Virus (ASLV) RTs (α and/or β subunits thereof), to isolated nucleic acid molecules encoding such RSV RT, AMV RT or other ASLV RT subunits, to vectors and host cells comprising these isolated nucleic acid molecules and to RSV RT, AMV RT and other ASLV RT subunits produced by these methods. The invention further relates to nucleic acid molecules encoding recombinant heterodimeric RT holoenzymes, particularly heterodimeric RSV RTs, AMV RTs or other ASLV RTs (which may be αβ RTs, ββ RTs, or α RTs), vectors (particularly baculovirus vectors) and host cells (particularly insect and yeast cells) comprising these nucleic acid molecules, methods for producing these heterodimeric RTs and heterodimeric RTs produced by these methods. The invention also relates to kits comprising the compositions, polypeptides, or RSV RTs, AMV RTs or other ASLV RTs of the invention.
摘要翻译：本发明通常涉及核酸分子，特别是信使RNA分子的逆转录的组合物和方法。具体地，本发明涉及包含具有逆转录酶（RT）活性的多肽的混合物的组合物，以及使用这些组合物或多肽，特别是在高于约55℃的温度下产生，扩增或测序核酸分子（特别是cDNA分子）的方法本发明还涉及通过这些方法产生的核酸分子，涉及包含这些核酸分子的载体和宿主细胞，以及使用这种核酸分子产生所需多肽。本发明还涉及用于生产分离的编码这样的核酸分子的Rous肉瘤病毒（RSV）和禽成髓细胞病毒（AMV）RT或其他禽类肉瘤 - 白血病病毒（ASLV）RT（其α和/或β亚基））的方法 RSV RT，AMV RT或其他ASLV RT亚单位，包含这些分离的核酸分子的载体和宿主细胞以及通过这些方法产生的RSV RT，AMV RT和其他ASLV RT亚基。本发明还涉及编码重组异源二聚体RT全酶，特别是异二聚体RSV RT，AMV RT或其他ASLV RT（其可以是字母表RT，betabeta RT或αRT），载体（特别是杆状病毒载体）和宿主细胞（特别是昆虫和酵母细胞），通过这些方法产生的这些异源二聚体RT和异二聚体RT的制备方法。本发明还涉及包含本发明的组合物，多肽或RSV RT，AMV RT或其他ASLV RT的试剂盒。

9. 发明申请

US20060134739A1 Synthesis of proteins by cell-free protein expression 审中-公开
标题翻译：通过无细胞蛋白表达合成蛋白质
公开(公告)号：US20060134739A1
公开(公告)日：2006-06-22
申请号：US10548634
申请日：2004-03-10
申请人： Deb Chatterjee
发明人： Deb Chatterjee
IPC分类号： C12P21/06 , C12N1/21
CPC分类号： C12P21/02 , C12P19/34
摘要： In Vitro peptide/protein or biological macromolecule synthesis systems, and methods and kits thereof improve efficiency of in vitro protein or biological macromolecule synthesis and related compositions.
摘要翻译：体外肽/蛋白质或生物大分子合成系统及其方法和试剂盒提高体外蛋白质或生物大分子合成和相关组合物的效率。

10. 发明申请

US20050123905A1 Methods and compositions for cloning amplified nucleic acid molecules 有权
标题翻译：用于克隆扩增的核酸分子的方法和组合物
公开(公告)号：US20050123905A1
公开(公告)日：2005-06-09
申请号：US10960510
申请日：2004-10-08
申请人： Donna Fox , Deb Chatterjee
发明人： Donna Fox , Deb Chatterjee
IPC分类号： A61K38/00 , C07K16/40 , C12N15/10 , C12N15/70 , C12Q1/70 , C12P19/34 , C12Q1/68
CPC分类号： C07K16/40 , A61K38/00 , C12N15/10 , C12N15/70
摘要： The present invention is directed generally to methods facilitating the cloning of nucleic acid molecules. In particular, the invention relates to the use of polymerase inhibitors, including but not limited to anti-polymerase antibodies (such as anti-Taq antibodies) and fragments thereof, to inactivate residual polymerase activity remaining after the amplification (particularly via PCR) of a target nucleic acid molecule. The invention further provides compositions, particularly storage-stable compositions, comprising one or more components, such as one or more restriction endonucleases and one or more polymerase inhibitors, that are useful in cloning amplified or synthesized nucleic acid molecules by the above-described methods. The invention also relates to nucleic acid molecules produced by these methods, and to genetic constructs (such as vectors) and host cells comprising these nucleic acid molecules.
摘要翻译：本发明一般涉及促进核酸分子克隆的方法。特别地，本发明涉及使用聚合酶抑制剂，包括但不限于抗聚合酶抗体（例如抗Taq抗体）及其片段，以使经扩增（特别是经PCR）后残留的残留聚合酶活性失活靶核酸分子。本发明还提供组合物，特别是储存稳定的组合物，其包含一种或多种组分，例如一种或多种限制性内切核酸酶和一种或多种聚合酶抑制剂，其可用于通过上述方法克隆扩增或合成的核酸分子。本发明还涉及通过这些方法产生的核酸分子以及包含这些核酸分子的遗传构建体（例如载体）和宿主细胞。

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