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    • 3. 发明申请
    • Engineered Biological Matrices
    • 工程生物矩阵
    • US20070141105A1
    • 2007-06-21
    • US11539580
    • 2006-10-06
    • Thomas SteinMark PowersJonathan CooperSorin DamianPieter Johannes HuibertsKenneth Guiseley
    • Thomas SteinMark PowersJonathan CooperSorin DamianPieter Johannes HuibertsKenneth Guiseley
    • A61F2/02A61K9/14C12N5/02
    • A61L27/52A61K47/6903A61L27/50A61L27/54A61L2300/604C12N5/0068C12N2533/76
    • Biocompatible matrices or implants on which one or more specific cell-interactive molecules (“biomolecules”) can be immobilized have been developed. The matrices allow for the independent control of ligand concentration and matrix strength. In one embodiment, the matrix or implant is modified with one or more moieties capable of complexing bioconjugates prepared from one or more biomolecules. Suitable moieties include phenyl boronic acid complexing agents, such as salicylhydroxamic acid, which can complex to one or more biomolecules containing one or more phenyl boronic acid moieties. The biomolecules may be anchored to the matrix via a spacer molecule, which may allow for greater mobility of the biomolecules in aqueous solution. In one embodiment, the matrix is a hydrogel material which has been doubly-derivatized, wherein ligand concentration and matrix strength can be independently controlled. The matrices and implants can be used in vivo and in vitro applications including diagnostics, biosensors, bioprocess engineering, tissue engineering, regeneration and repair, and drug delivery.
    • 已经开发了可以固定有一个或多个特定细胞相互作用分子(“生物分子”)的生物相容性基质或植入物。 基质允许独立控制配体浓度和基质强度。 在一个实施方案中,基质或植入物用能够络合由一种或多种生物分子制备的生物缀合物的一个或多个部分修饰。 合适的部分包括苯基硼酸络合剂,例如水杨酸异羟肟酸,其可与含有一个或多个苯基硼酸部分的一种或多种生物分子络合。 生物分子可以通过间隔分子锚定到基质,这可以允许生物分子在水溶液中更大的迁移率。 在一个实施方案中,基质是双重衍生的水凝胶材料,其中配体浓度和基质强度可以独立控制。 基质和植入物可用于体内和体外应用,包括诊断,生物传感器,生物过程工程,组织工程,再生和修复以及药物递送。