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1. 发明申请

US20050289345A1 Method and system for providing a document which can be visually authenticated 审中-公开
标题翻译：提供可直观认证的文件的方法和系统
公开(公告)号：US20050289345A1
公开(公告)日：2005-12-29
申请号：US10996015
申请日：2004-11-23
申请人： David Haas , Robert Holt
发明人： David Haas , Robert Holt
IPC分类号： G07D7/12 , G07D7/20 , H04L9/00
CPC分类号： G09C5/00 , G07D7/003 , G07D7/206
摘要： A method of providing a document which can be visually authenticated includes providing a document that is valid for a predetermined period of validity; randomly selecting visual authentication indicia just prior to the period of validity, the visual authentication indicia providing visual indication of validity during the period of validity; and transferring the visual authentication indicia to the document to visually authenticate the validity of the document during the period of validity. The method can be implemented using a system for creating a document which can be visually authenticated disclosed herein. The system includes a plurality of possible visual authentication indicia; a computer capable of executing instructions for randomly selecting one of the plurality of possible visual authentication indicia just prior to a predetermined period of validity; and at least one document validation station for transferring the selected visual authentication indicia to a document to visually authenticate the validity of the document during the period of validity.
摘要翻译：提供可视觉认证的文档的方法包括提供在预定有效期内有效的文档; 在有效期之前随机选择视觉认证标记，视觉认证标记在有效期内提供有效性的视觉指示; 并将视觉认证标记转移到文档，以在有效期内视觉上验证文档的有效性。该方法可以使用用于创建可以在此公开的视觉认证的文档的系统来实现。该系统包括多个可能的视觉认证标记; 能够在预定有效期之前执行随机选择多个可能的视觉认证标记中的一个的指令的计算机; 以及至少一个文档验证站，用于将所选择的可视认证标记传送到文档以在有效期内视觉上验证文档的有效性。

2. 发明申请

US20050226101A1 Time indicator assembly 有权
标题翻译：时间指示器组合
公开(公告)号：US20050226101A1
公开(公告)日：2005-10-13
申请号：US10819791
申请日：2004-04-07
申请人： David Haas , Robert Holt
发明人： David Haas , Robert Holt
IPC分类号： G04F1/06 , G04F13/06 , G04C1/00
CPC分类号： G04F13/06
摘要： A time indicator assembly including a front part and a back part is disclosed that can easily pass through a printer. The time indicator assembly includes a base substrate forming at least part of the back part having a first surface and a second surface extending between edges. A migrating ink is applied over a portion of the first surface of the base substrate, and a release sheet overlies the migrating ink to prevent migration of the migrating ink toward the front part. In one embodiment, an adhesive is applied over the release sheet and onto the base substrate adjacent at least two of the base substrate edges. The adhesive joins a cover substrate forming at least part of the front part to the base substrate over at least a portion of the release sheet and along the at least two of the base substrate edges. Upon removal of the release sheet, the migrating ink migrates through the adhesive to form an image in the cover substrate. In another embodiment a lift strip is formed in at least one of the front part and back part for easily separating the front part and back part to expose and remove the release sheet.
摘要翻译：公开了一种包括前部和后部的时间指示器组件，其可以容易地通过打印机。时间指示器组件包括形成背部的至少一部分的基底基底，其具有第一表面和在边缘之间延伸的第二表面。将迁移的油墨施加在基底基材的第一表面的一部分上，并且脱模片覆盖迁移的油墨，以防止迁移的油墨向前部移动。在一个实施方案中，将粘合剂施加到隔离片上并附着在与基底基材边缘中的至少两个相邻的基底基板上。粘合剂将形成至少部分前部的覆盖基材连接到脱模片的至少一部分上并且沿着至少两个基底边缘的基底基底。在去除脱模片时，迁移的油墨通过粘合剂迁移以在盖基材中形成图像。在另一个实施例中，在前部和后部中的至少一个中形成提升带，用于容易地分离前部和后部以露出和移除剥离片。

3. 发明申请

US20050185520A1 Long term rapid color changing time indicator 失效
标题翻译：长期快速变色时间指示器
公开(公告)号：US20050185520A1
公开(公告)日：2005-08-25
申请号：US10786449
申请日：2004-02-25
申请人： David Haas , Robert Holt , Leonard Davis
发明人： David Haas , Robert Holt , Leonard Davis
IPC分类号： G04F1/00 , G04F1/06 , G04F13/06 , G04C1/00
CPC分类号： G04F13/06 , G04F1/06
摘要： A long term rapid color changing time indicator includes a front part and a back part. The front part includes a transparent layer, an opaque layer, a colorant layer, and a neutralizing layer. The colorant layer includes a matrix and a colorant having a non-migratory form that does not migrate in the matrix and having a migratory form that migrates in the matrix. The back part has a reactant. When the front part and the back part are placed in contact, the reactant migrates into the neutralizing layer and a portion of the reactant is depleted by a coreactant. The unreacted reactant migrates into the colorant layer and reacts with the non-migratory form of the colorant converting the non-migratory form to the migratory form such that the migratory form of the colorant migrates through the opaque layer to cause a visual color indication in the transparent layer.
摘要翻译：长期快速变色时间指示器包括前部和后部。前部包括透明层，不透明层，着色层和中和层。着色剂层包括基质和具有非迁移形式的着色剂，其不会在基质中迁移并具有在基质中迁移的迁移形式。后部具有反应物。当前部和后部接触时，反应物迁移到中和层中，并且一部分反应物被共反应物耗尽。未反应的反应物迁移到着色剂层中，并与非迁移形式的着色剂反应，将非迁移形式转化为迁移形式，使得着色剂的迁移形式通过不透明层迁移以引起视觉颜色指示透明层。

4. 发明授权

US09873118B2 Biologic fluid analysis cartridge with sample handling portion and analysis chamber portion 有权
公开(公告)号：US09873118B2
公开(公告)日：2018-01-23
申请号：US13341618
申请日：2011-12-30
申请人： John A. Verrant , Niten V. Lalpuria , Igor Nikonorov , Darryn W. Unfricht , Benjamin Ports , Stephen C. Wardlaw , Robert A. Levine , Robert Holt , Kyle Hukari
发明人： John A. Verrant , Niten V. Lalpuria , Igor Nikonorov , Darryn W. Unfricht , Benjamin Ports , Stephen C. Wardlaw , Robert A. Levine , Robert Holt , Kyle Hukari
IPC分类号： A61J1/06 , B01L3/00
CPC分类号： B01L3/502707 , B01L3/5027 , B01L3/50273 , B01L2200/027 , B01L2300/0861
摘要： A biological fluid analysis cartridge is provided. In certain embodiments, the cartridge includes a base plate extending between a sample handling portion and an analysis chamber portion. A handling upper panel is attached to the base plate within the sample handling portion. A collection port is at least partially formed with the handling upper panel. An initial channel and a secondary channel are formed between the handling upper panel and the base plate. The collection port and initial and secondary channels are in fluid communication with one another. A chamber upper panel is attached to the base plate within the analysis chamber portion. At least one analysis chamber is formed between the chamber upper panel and the base plate. The secondary channel and the analysis chamber are in fluid communication with one another.

5. 发明申请

US20130259899A1 DETECTION OF FUSOBACTERIUM IN A GASTROINTESTINAL SAMPLE TO DIAGNOSE GASTROINTESTINAL CANCER 审中-公开
标题翻译：检测胃肠道样品中胆固醇对胃肠道癌的诊断
公开(公告)号：US20130259899A1
公开(公告)日：2013-10-03
申请号：US13877421
申请日：2011-10-04
申请人： Emma Allen-Vercoe , Robert Holt , Richard Moore , Rene Warren
发明人： Emma Allen-Vercoe , Robert Holt , Richard Moore , Rene Warren
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6886 , A61K39/114 , C12Q1/689 , C12Q2600/118 , C12Q2600/136 , G01N33/56911 , G01N33/57419 , G01N33/57446 , G01N2333/195
摘要： Fusobacterium is a genus of gram-negative, filamentous, anaerobic bacteria found as normal flora in the mouth and large bowel, and often in necrotic tissue. A comparison of microbial ribonucleic acids (RNA) between colorectal carcinoma (CRC) tissue and adjacent normal control tissue found the over-representation of F. nucleatum in CRC tissue. RNA abundance was measured by polymerase chain reaction (PCR)-amplifying RNA from the tissue, constructing libraries, sequencing the RNA in the libraries, pairing sequences from CRC and normal tissue, and quantifying RNA abundance. Detection of Fusobacterium in a gastrointestinal sample is indicative of gastrointestinal cancer.
摘要翻译：梭菌属是革兰氏阴性，丝状，厌氧菌，属于口腔大肠正常菌群，常见于坏死组织。结肠直肠癌（CRC）组织和相邻正常对照组织中微生物核糖核酸（RNA）的比较发现CRC组织中F.核仁的过度表达。通过聚合酶链反应（PCR） - 从组织中扩增RNA，构建文库，对文库中的RNA进行测序，从CRC和正常组织配对序列以及定量RNA丰度来测量RNA丰度。胃肠道样品中的梭菌的检测是胃肠癌的指示。

6. 发明授权

US06942975B2 Methods and kits for obtaining DNA end sequence information for large cloned inserts and uses thereof 失效
标题翻译：用于获得大型克隆插入物的DNA末端序列信息的方法和试剂盒及其用途
公开(公告)号：US06942975B2
公开(公告)日：2005-09-13
申请号：US10243738
申请日：2002-09-16
申请人： Robert Holt , Hamilton O. Smith , Cynthia Pfannkoch
发明人： Robert Holt , Hamilton O. Smith , Cynthia Pfannkoch
IPC分类号： C12N15/65 , C12N15/66 , C12Q1/68 , G01N33/53
CPC分类号： C12N15/66 , C12N15/65 , C12Q1/6869
摘要： The present invention provides a method of producing a cloned insert that is representative of the ends of a large segment of DNA from the genome of an organism. Specifically, the present invention provides a clone insert strategy that comprising the steps of: a) isolating a nucleic acid molecule, or portion thereof, wherein said nucleic acid molecule is at least 20 kb; b) ligating said nucleic acid molecule into a vector molecule; c) cutting said nucleic acid molecule with a restriction endonuclease, wherein said restriction endonuclease cuts said isolated nucleic acid molecule of step (a) in at least two sites but does not cut said vector DNA, d) ligating said cut nucleic acid molecule; e) transforming a host cell with said ligated nucleic acid molecule to propagate said molecule; f) determining the sequence of the ends of the nucleic acid insert and the sequence from the restriction endonculease site.
摘要翻译：本发明提供了一种克隆插入物的方法，该插入物代表来自生物体的基因组DNA的大片段的末端。具体地说，本发明提供克隆插入策略，其包括以下步骤：a）分离其中所述核酸分子至少为20kb的核酸分子或其部分; b）将所述核酸分子连接到载体分子中; c）用限制性内切核酸酶切割所述核酸分子，其中所述限制性内切核酸酶在至少两个位点切割步骤（a）的所述分离的核酸分子，但不切割所述载体DNA，d）连接所述切割的核酸分子; e）用所述连接的核酸分子转化宿主细胞以繁殖所述分子; f）确定核酸插入物的末端序列和来自限制性内切酶位点的序列。

7. 发明授权

US06452873B1 Visually changing paper time indicator employing controllable barrier 失效
标题翻译：使用可控屏障视觉更换纸张时间指示器
公开(公告)号：US06452873B1
公开(公告)日：2002-09-17
申请号：US09593854
申请日：2000-06-14
申请人： Robert Holt , David J. Haas
发明人： Robert Holt , David J. Haas
IPC分类号： G04B1700
CPC分类号： G04F1/00
摘要： A time indicator rapidly changes color after a specific time interval. The indicator comprises “back part” that includes a base substrate with a colored dye or colorant deposited on a first surface. A colorant impermeable barrier layer overlays the colorant or colored dye. The indicator further comprises a “front part” that includes a substrate having an adhesive on a first surface thereof. When the back part and front part are put into adhesive contact with each other, the parts coact with each other to cause the colorant impermeable barrier layer to change to a colorant permeable layer to permit the dye to migrate through the layers to cause a color change visible through the front part.
摘要翻译：时间指示器在特定时间间隔后快速更改颜色。指示器包括“后部”，其包括具有沉积在第一表面上的着色染料或着色剂的基底。着色剂不可渗透阻挡层覆盖着色剂或着色染料。指示器还包括“前部”，其包括在其第一表面上具有粘合剂的基底。当后部和前部彼此粘合接触时，这些部分彼此共同作用，使着色剂不可渗透的阻隔层变成着色剂可渗透层，以使染料迁移通过层以引起颜色变化通过前部可见。

8. 发明申请

US20110129394A1 INSPECTION AND TESTING INDICATOR 有权
标题翻译：检验和测试指标
公开(公告)号：US20110129394A1
公开(公告)日：2011-06-02
申请号：US13012954
申请日：2011-01-25
申请人： Robert Holt , David J. Haas
发明人： Robert Holt , David J. Haas
IPC分类号： G01N21/78
CPC分类号： G04F1/00
摘要： An indicator is disclosed that detects the presence of a chemical residue. The indicator includes a substrate having an upper surface and a lower surface and a first portion and a second portion joined at a fold line. The first portion is smaller in area than the second portion. An adhesive coats the upper surface of at least the second portion of the substrate. A first reactant is adhered to the upper surface of the first portion of the substrate. When the second portion is contacted with a surface containing the chemical residue, the residue adheres to the adhesive and when the first portion is subsequently folded along the fold line so that the upper surfaces of the substrate contact each other, a portion of the adhesive on the second portion remains exposed and the first reactant and chemical residue react to provide the color indicia indicating the presence of the chemical residue.
摘要翻译：公开了一种检测化学残留物的存在的指示剂。指示器包括具有上表面和下表面的基底，以及在折叠线处连接的第一部分和第二部分。第一部分的面积小于第二部分。粘合剂涂覆至少衬底的第二部分的上表面。第一反应物粘附到基底的第一部分的上表面。当第二部分与含有化学残留物的表面接触时，残余物粘附到粘合剂上，并且当第一部分随后沿折叠线折叠，使得基材的上表面彼此接触时，粘合剂的一部分第二部分保持暴露，并且第一反应物和化学残留物反应以提供指示化学残留物的存在的颜色标记。

9. 发明授权

US07898907B1 Inspection and testing indicator 有权
标题翻译：检验检测指标
公开(公告)号：US07898907B1
公开(公告)日：2011-03-01
申请号：US10674882
申请日：2003-09-30
申请人： Robert Holt , David J. Haas
发明人： Robert Holt , David J. Haas
IPC分类号： G04B17/00 , G01D21/00
CPC分类号： G04F1/00
摘要： A time indicator that provides a color indicia after a predetermined period of time has passed after activation. The time indicator includes a substrate, e.g., a clear or transparent substrate, having an upper surface and a lower surface and a first portion and a second portion joined at a fold line. The first portion is of a smaller area than the second portion. An adhesive coats the upper surface of at least the second portion of the substrate. A first reactant is adhered to the upper surface of the first portion of the substrate and a second reactant is adhered to the upper surface of the second portion of the substrate. When the first portion is folded along the fold line so that the upper surfaces of the substrate contact each other, a portion of the adhesive on the second portion remains exposed. This exposed area may be used to attach the indicator to an article or documents. When so folded the first reactant and second reactant contact each other to activate the indicator and to provide the color indicia after the predetermined period of time after activation passes. In one embodiment, the indicator is used to detect the presence of a chemical residue, e.g., explosive compound residues.
摘要翻译：在激活之后经过预定时间段之后提供颜色标记的时间指示器已经过去。时间指示器包括具有上表面和下表面的基底，例如透明或透明基底，以及在折叠线处连接的第一部分和第二部分。第一部分的面积比第二部分小。粘合剂涂覆至少衬底的第二部分的上表面。第一反应物粘附到基材的第一部分的上表面，并且第二反应物粘附到基材的第二部分的上表面。当第一部分沿着折叠线折叠以使得基板的上表面彼此接触时，第二部分上的粘合剂的一部分保持暴露。该暴露区域可用于将指示器附接到物品或文件。当如此折叠时，第一反应物和第二反应物彼此接触以激活指示剂，并且在激活通过之后的预定时间段之后提供颜色标记。在一个实施方案中，指示剂用于检测化学残留物（例如爆炸性化合物残留物）的存在。

10. 发明申请

US20070243617A1 Modular genomes for synthetic biology and metabolic engineering 审中-公开
标题翻译：用于合成生物学和代谢工程的模块化基因组
公开(公告)号：US20070243617A1
公开(公告)日：2007-10-18
申请号：US11546609
申请日：2006-10-12
申请人： Robert Holt
发明人： Robert Holt
IPC分类号： C12N1/21 , C07H21/04 , C12N15/00 , C12N15/74
CPC分类号： C12N15/90 , C12N15/70 , C12N2800/30
摘要： The invention provides methods and compositions for assembling a modular replacement genome in a host microorganism. After such assembly, the host organism's genome is inactivated or ablated to permit full control of host cellular functions by the replacement genome. A modular replacement genome comprises an assembly of nucleic acid fragments, or segments, derived from one or more natural organisms or from synthetic polynucleotides or from a combination of both. Such an assembly, or set, of segments making up a replacement genome comprises a substantially complete set of genes and regulatory elements for carrying out minimal life functions under predefined culture conditions. The invention provides modular genomes having modules that are amenable to facile replacement, deletion, and/or additions. Such modules may be synthetic polynucleotides and may be designed for controlling gene content, excluding of genes that encode inhibitors or otherwise undesirable competing enzymes that divert a host cell from desired metabolic/synthetic processes; modifying codon usage to maximize or minimize protein production; modifying regulatory elements, including promoters, enhancers, repressors, activator, or the like, to modulate gene expression; balancing enzymatic and transport activities to optimize fluxes of substrates, intermediates, and products in metabolic pathways, and like objectives.
摘要翻译：本发明提供了用于在宿主微生物中组装模块替代基因组的方法和组合物。在这种装配之后，宿主生物体的基因组被灭活或消融以允许通过置换基因组完全控制宿主细胞功能。模块化替代基因组包含衍生自一种或多种天然生物体或来自合成多核苷酸或两者的组合的核酸片段或片段组合。构成替代基因组的片段或组合的组合包括用于在预定培养条件下进行最小寿命功能的基本完整的基因和调控元件。本发明提供了模块化基因组，其具有易于更换，删除和/或添加的模块。这样的模块可以是合成多核苷酸，并且可以设计用于控制基因含量，排除编码抑制剂的基因或以其它方式将宿主细胞从期望的代谢/合成过程转移的不希望的竞争性酶; 修改密码子使用以最大化或最小化蛋白质生产; 修饰调节元件，包括启动子，增强子，阻遏物，活化剂等，以调节基因表达; 平衡酶和运输活动以优化底物，中间体和代谢途径中的产物的通量以及类似目标。

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