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1. 发明授权

US06936462B1 High throughput screen 有权
公开(公告)号：US06936462B1
公开(公告)日：2005-08-30
申请号：US09719236
申请日：1999-06-14
申请人： David Geraint Owen , Nicholas Gerard Byrne
发明人： David Geraint Owen , Nicholas Gerard Byrne
IPC分类号： G01N33/50 , G01N33/543 , G01N33/68 , G01N37/00 , C12M1/34 , C12M3/00
CPC分类号： G01N33/502 , B01L3/5025 , B01L2300/0829 , B01L2300/0851 , B01L2300/0893 , B01L2300/16 , G01N27/28 , G01N33/48728 , G01N33/5438 , G01N33/6872 , G01N2500/04 , G01N2500/10 , Y10T29/49826
摘要： The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.

2. 发明授权

US08449825B2 High throughput screen 有权
标题翻译：高通量屏幕
公开(公告)号：US08449825B2
公开(公告)日：2013-05-28
申请号：US12940564
申请日：2010-11-05
申请人： David Geraint Owen , Nicholas Gerard Byrne
发明人： David Geraint Owen , Nicholas Gerard Byrne
IPC分类号： G01N27/00
CPC分类号： G01N33/502 , B01L3/5025 , B01L2300/0829 , B01L2300/0851 , B01L2300/0893 , B01L2300/16 , G01N27/28 , G01N33/48728 , G01N33/5438 , G01N33/6872 , G01N2500/04 , G01N2500/10 , Y10T29/49826
摘要： The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.
摘要翻译：本发明涉及一种包括生物膜和多孔或穿孔基底，生物膜，基底，高通量筛选，结构膜和基底的制备方法以及筛选大量试验的方法的结构化合物在短时间内。更具体地说，涉及一种结构，该结构包括附着于多孔或多孔基底的生物膜，能够将高电阻密封粘附到诸如多孔玻璃的基底上的生物膜，以及形成主要具有离子通道或转运体兴趣，用于确定测试化合物对离子通道或转运蛋白活性的影响的高通量筛选，用于制造结构，膜和底物的方法，以及用于监测膜中的离子通道或转运蛋白活性的方法。

3. 发明申请

US20110048939A1 High Throughput Screen 有权
标题翻译：高通量屏幕
公开(公告)号：US20110048939A1
公开(公告)日：2011-03-03
申请号：US12940564
申请日：2010-11-05
申请人： David Geraint Owen , Nicholas Gerard Byrne
发明人： David Geraint Owen , Nicholas Gerard Byrne
IPC分类号： G01N27/403
CPC分类号： G01N33/502 , B01L3/5025 , B01L2300/0829 , B01L2300/0851 , B01L2300/0893 , B01L2300/16 , G01N27/28 , G01N33/48728 , G01N33/5438 , G01N33/6872 , G01N2500/04 , G01N2500/10 , Y10T29/49826
摘要： The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on ion channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.
摘要翻译：本发明涉及一种包括生物膜和多孔或穿孔基底，生物膜，基底，高通量筛选，结构膜和基底的制备方法以及筛选大量试验的方法的结构化合物在短时间内。更具体地说，涉及一种结构，该结构包括附着于多孔或多孔基底的生物膜，能够将高电阻密封粘附到诸如多孔玻璃的基底上的生物膜，以及形成主要具有离子通道或转运体兴趣，用于确定测试化合物对离子通道或转运蛋白活性的影响的高通量筛选，用于制造结构，膜和底物的方法，以及用于监测膜中的离子通道或转运蛋白活性的方法。

4. 发明授权

US07846389B2 High throughput screen 有权
标题翻译：高通量屏幕
公开(公告)号：US07846389B2
公开(公告)日：2010-12-07
申请号：US11133808
申请日：2005-05-20
申请人： David Geraint Owen , Nicholas Gerard Byrne
发明人： David Geraint Owen , Nicholas Gerard Byrne
IPC分类号： G01N33/567 , G01N27/00 , G01N27/07 , G01N27/08 , B01L3/00 , B01L9/00
CPC分类号： G01N33/502 , B01L3/5025 , B01L2300/0829 , B01L2300/0851 , B01L2300/0893 , B01L2300/16 , G01N27/28 , G01N33/48728 , G01N33/5438 , G01N33/6872 , G01N2500/04 , G01N2500/10 , Y10T29/49826
摘要： The present invention relates to a structure comprising a biological membrane and a porous or perforated substrate, a biological membrane, a substrate, a high throughput screen, methods for production of the structure membrane and substrate, and a method for screening a large number of test compounds in a short period. More particularly it relates to a structure comprising a biological membrane adhered to a porous or perforated substrate, a biological membrane capable of adhering with high resistance seals to a substrate such as perforated glass and the ability to form sheets having predominantly an ion channel or transporter of interest, a high throughput screen for determining the effect of test compounds on inn channel or transporter activity, methods for manufacture of the structure, membrane and substrate, and a method for monitoring ion channel or transporter activity in a membrane.
摘要翻译：本发明涉及一种包括生物膜和多孔或穿孔基底，生物膜，基底，高通量筛选，结构膜和基底的制备方法以及筛选大量试验的方法的结构化合物在短时间内。更具体地说，涉及一种结构，该结构包括附着于多孔或多孔基底的生物膜，能够将高电阻密封粘附到诸如多孔玻璃的基底上的生物膜，以及形成主要具有离子通道或转运体感兴趣的，用于确定测试化合物对宿主通道或转运蛋白活性的影响的高通量筛选，用于制造结构，膜和底物的方法以及用于监测膜中的离子通道或转运蛋白活性的方法。

5. 发明授权

US07384733B1 Interface patch clamping 失效
标题翻译：接口补片夹
公开(公告)号：US07384733B1
公开(公告)日：2008-06-10
申请号：US09857456
申请日：1999-12-06
申请人： Nicholas Gerard Byrne , David Geraint Owen
发明人： Nicholas Gerard Byrne , David Geraint Owen
IPC分类号： C12Q1/00 , C12Q1/02 , C12M1/34 , C12M3/00
CPC分类号： G01N33/48728 , C12M41/46
摘要： The invention provides a novel development of the conventional patch clamp technique for measurement of whole cell electrical activity. The invention provides for one or more cell or cells to be suspended in a liquid medium at a liquid/air interface (by virtue of the effect of surface tension at the interface) whereby the cell or cells are accessible at the interface to a microstructure electrode (such as a pipette tip) to which a cell can attach to form an electrical seal, for the purpose of whole cell voltage clamp recording. According to the invention the electrode can be caused to form a high resistance electrical seal with a cell suspended in the liquid at the liquid/air interface without the need to press the cell against a solid support surface. The invention also provides apparatus for carrying out the interface patch clamp technique and control logic for operating a computer to carry out the interface patch clamp technique.
摘要翻译：本发明提供了用于测量全细胞电活动的常规膜片钳技术的新发展。本发明提供一种或多种在液体/空气界面处悬浮在液体介质中的细胞或细胞（借助于界面处的表面张力的作用），由此在与微结构电极的界面处可接触细胞或细胞（例如移液管尖端），电池可以附接到其上以形成电气密封，用于全电池电压钳记录的目的。根据本发明，可以使电极与在液体/空气界面处悬浮在液体中的电池形成高电阻电气密封，而不需要将电池压在固体支撑表面上。本发明还提供了用于执行界面膜片钳技术的装置和用于操作计算机以执行界面膜片钳技术的控制逻辑。

6. 发明授权

US5965696A Potassium channel modulators 失效
标题翻译：钾通道调节剂
公开(公告)号：US5965696A
公开(公告)日：1999-10-12
申请号：US451371
申请日：1995-05-26
申请人： Roger Crossley , Albert Opalko , David Geraint Owen , Brian Robertson
发明人： Roger Crossley , Albert Opalko , David Geraint Owen , Brian Robertson
IPC分类号： A61K38/00 , C07K14/705 , C07K5/00 , C07K7/00
CPC分类号： C07K14/705 , A61K38/00
摘要： This invention concerns peptides (I) having intracellular potassium channel modulating activity comprising the amino acid sequence shown in SEQ ID No: 2:(N terminal function) (I) Met Ile Ser Ser Val Cys Val Ser Ser 1 5} Tyr Arg Gly Arg Lys Ser Gly Asn Lys 10 15 Pro Pro Ser Lys Thr Cys Leu Lys Glu Glu 20 25 (C terminal function) in which the cysteines are optionally linked via a disulphide bridge and wherein______________________________________ Met represents L-methionine Ile represents L-isoleucine Ser represents L-serine Val represents L-valine Cys represents L-cysteine Tyr represents L-tyrosine Arg represents L-arginine Gly represents glycine Lys represents L-lysine Asn represents L-asparagine Pro represents L-proline Thr represents L-threonine Leu represents L-leucine Glu represents L-glutamic acid ______________________________________ or a variant thereof,with the proviso that excluded is the 13-Lys variant (where 13-Arg is replaced by 13-Lys) in which the cysteines are not linked via a disulphide bridge, which are useful in a test method of screening for compounds having potassium channel modulating activity.
摘要翻译：本发明涉及具有包含SEQ ID No：2所示氨基酸序列的细胞内钾通道调节活性的肽（I）： - （N末端功能）（I） - - Met Ile Ser Ser Val Cys Val Ser Ser-15} - - Tyr Arg Gly Arg Lys Ser Gly Asn Lys - 10 15 - Pro Pro Ser Lys Thr Cys Leu Lys Glu Glu - 20 25 - - （C末端功能） - 其中半胱氨酸任选通过二硫键连接，其中 - Met表示L-甲硫氨酸 - Ile表示L-异亮氨酸 - Ser表示L-丝氨酸 - Val表示L-缬氨酸 - Cys表示L-半胱氨酸-Tyr表示L-酪氨酸-Arg表示L-精氨酸-Gly表示甘氨酸 - Lys表示L - 赖氨酸 - Asn代表L-天冬酰胺-Pro代表L-脯氨酸 - Thr代表L-苏氨酸-Leu表示L-亮氨酸-Glu表示L-谷氨酸 - 或其变体，条件是排除的是13-Lys变体（其中13-Arg被13-Lys替代），其中半胱氨酸不通过二硫键连接桥，其可用于筛选具有钾通道调节活性的化合物的测试方法。

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