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1. 发明授权

US08334112B2 Medium for detecting and/or identifying bacteria 有权
标题翻译：用于检测和/或识别细菌的培养基
公开(公告)号：US08334112B2
公开(公告)日：2012-12-18
申请号：US12448895
申请日：2008-02-07
申请人： Daniel Monget , Sylvain Orenga , John Perry , Michel Peyret , Celine Roger-Dalbert
发明人： Daniel Monget , Sylvain Orenga , John Perry , Michel Peyret , Celine Roger-Dalbert
IPC分类号： C12Q1/10 , C12Q1/04 , C12Q1/02 , C12N1/20
CPC分类号： C12Q1/10
摘要： The invention relates to a method for detecting and/or identifying Escherichia coli (E. coli) in a biological sample, that comprises inoculating the biological sample liable to contain E. coli on a detection medium that comprises tryptophan and a substrate for an enzyme A, expressed by the majority of E. coli, in order to obtain bacterial colonies; detecting the colonies expressing the activity of the enzyme A and identifying them as being E. coli; and detecting the colonies that do not express the activity of the enzyme A, carrying out an indole test, and identifying the colonies having a positive indole test as being E. coli.
摘要翻译：本发明涉及用于在生物样品中检测和/或鉴定大肠杆菌（E.coli）的方法，其包括在包含色氨酸和酶A底物的检测培养基上接种容易含有大肠杆菌的生物样品，由大多数大肠杆菌表达，以获得细菌菌落; 检测表达酶A活性的菌落，并将其鉴定为大肠杆菌; 并检测不表达酶A的活性的菌落，进行吲哚试验，并鉴定具有阳性吲哚试验的菌落为大肠杆菌。

2. 发明申请

US20100062467A1 Medium For Detecting And/Or Identifying Bacteria 有权
标题翻译：用于检测和/或识别细菌的培养基
公开(公告)号：US20100062467A1
公开(公告)日：2010-03-11
申请号：US12448895
申请日：2008-02-07
申请人： Daniel Monget , Sylvain Orenga , John Perry , Michel Peyret , Celine Roger-Dalbert
发明人： Daniel Monget , Sylvain Orenga , John Perry , Michel Peyret , Celine Roger-Dalbert
IPC分类号： C12Q1/34 , C12Q1/44 , C12Q1/37 , C12Q1/04
CPC分类号： C12Q1/10
摘要： The invention relates to a method for detecting and/or identifying Escherichia coli (E. coli) in a biological sample, that comprises inoculating the biological sample liable to contain E. coli on a detection medium that comprises tryptophan and a substrate for an enzyme A, expressed by the majority of E. coli, in order to obtain bacterial colonies; detecting the colonies expressing the activity of the enzyme A and identifying them as being E. coli; and detecting the colonies that do not express the activity of the enzyme A, carrying out an indole test, and identifying the colonies having a positive indole test as being E. coli.
摘要翻译：本发明涉及用于在生物样品中检测和/或鉴定大肠杆菌（E.coli）的方法，其包括在包含色氨酸和酶A底物的检测培养基上接种容易含有大肠杆菌的生物样品，由大多数大肠杆菌表达，以获得细菌菌落; 检测表达酶A活性的菌落，并将其鉴定为大肠杆菌; 并检测不表达酶A的活性的菌落，进行吲哚试验，并鉴定具有阳性吲哚试验的菌落为大肠杆菌。

3. 发明申请

US20100255530A1 BACTERIA DETECTION AND/OR IDENTIFICATION MEDIUM 审中-公开
标题翻译：细菌检测和/或鉴定中
公开(公告)号：US20100255530A1
公开(公告)日：2010-10-07
申请号：US12448823
申请日：2008-02-07
申请人： Daniel Monget , Sylvain Orenga , Michel Peyret , Celine Roger-Dalbert
发明人： Daniel Monget , Sylvain Orenga , Michel Peyret , Celine Roger-Dalbert
IPC分类号： C12Q1/10
CPC分类号： C12Q1/045 , C12Q1/10 , C12Q1/34 , C12Q2334/50 , G01N2333/245
摘要： The invention relates to a method for detecting and/or identifying E. coli in a urine sample, that comprises: a) inoculating the urine sample liable to contain E. coli on a detection medium that comprises a first substrate selected from a beta-glucuronidase substrate, beta-galactosidase substrate and alpha-galactosidase substrate, and a substrate for a lactose acidification enzyme, beta-ribosidase, phosphatase, L-alanine aminopeptidase and L-leucine aminopeptidase, and a second substrate, different from said first substrate and selected from a beta-glucuronidase substrate, beta-galactosidase substrate and alpha-galactosidase substrate, and a substrate for a lactose acidification enzyme, beta-ribosidase, phosphatase, L-alanine aminopeptidase and L-leucine aminopeptidase, or obtaining bacterial colonies; b) identifying the colonies that react with the first substrate and/or the second substrate as being colonies of E. coli.
摘要翻译：本发明涉及一种用于检测和/或鉴定尿液样本中大肠杆菌的方法，其包括：a）将含有大肠杆菌的尿液样本接种在检测培养基上，所述检测培养基包含选自β-葡糖苷酸酶底物，β-半乳糖苷酶底物和α-半乳糖苷酶底物，以及用于乳糖酸化酶，β-核糖酶，磷酸酶，L-丙氨酸氨基肽酶和L-亮氨酸氨基肽酶的底物，以及与所述第一底物不同的第二底物， β-葡糖醛酸酶底物，β-半乳糖苷酶底物和α-半乳糖苷酶底物，以及用于乳糖酸化酶，β-核糖酶，磷酸酶，L-丙氨酸氨基肽酶和L-亮氨酸氨基肽酶的底物，或获得细菌菌落; b）鉴定作为大肠杆菌菌落与第一底物和/或第二底物反应的菌落。

4. 发明申请

US20090017481A1 Method For Identifying at Least Two Groups of Microorganisms 有权
标题翻译：用于鉴定至少两组微生物的方法
公开(公告)号：US20090017481A1
公开(公告)日：2009-01-15
申请号：US12223025
申请日：2007-02-23
申请人： Sylvain Orenga , Celine Roger-Dalbert , Arthur James , John Perry
发明人： Sylvain Orenga , Celine Roger-Dalbert , Arthur James , John Perry
IPC分类号： C12Q1/44 , C12Q1/04 , C12Q1/37 , C12Q1/34
CPC分类号： C12Q1/04 , C12Q1/045
摘要： The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps: a) incubating said groups of microorganisms in a reaction medium comprising a first enzyme substrate and a second enzyme substrate, said first and second enzyme substrates being metabolized by the same enzymatic activity; b) identifying said groups of microorganisms.
摘要翻译：本发明涉及用于鉴定表达相同酶活性的至少两组微生物的方法，包括以下步骤：a）将所述微生物组在包含第一酶底物和第二酶底物的反应介质中孵育，所述第一并且第二酶底物被相同的酶活性代谢; b）鉴定所述微生物组。

5. 发明申请

US20060257966A1 Medium for detection and identification of micro-organisms 有权
标题翻译：用于微生物检测和鉴定的培养基
公开(公告)号：US20060257966A1
公开(公告)日：2006-11-16
申请号：US10552508
申请日：2004-04-02
申请人： Sylvain Orenga , Celine Roger-Dalbert , John Perry , Arthur James
发明人： Sylvain Orenga , Celine Roger-Dalbert , John Perry , Arthur James
IPC分类号： C12Q1/04
CPC分类号： C12Q1/045 , C12Q1/10
摘要： The invention relates to a medium for detecting and/or identifying microorganisms present in a sample, comprising a culture medium and at least one substrate that can be hydrolysed to a labelled product by at least a first enzyme not free in the sample, and specific for the microorganisms, characterized in that it also comprises at least one inhibitor of at least a second enzyme, different from the first enzyme or identical to it, but free in said sample and not originating from a microorganism. The present invention also finds a preferred application in the field of biomedical diagnosis or in food microbiology, and more particularly in bacteriology and mycology.
摘要翻译：本发明涉及一种用于检测和/或鉴定样品中存在的微生物的培养基，其包含培养基和至少一种可通过样品中不含游离的至少第一种酶水解成标记产物的底物，并且特异于所述微生物的特征在于，其还包含至少一种与第一种酶不同的第二种酶的至少一种抑制剂，或与之相同，但在所述样品中游离并且不来自微生物。本发明还发现在生物医学诊断或食品微生物学领域中的优选应用，更特别地在细菌学和真菌学领域。

6. 发明授权

US08420345B2 Method for identifying at least two groups of microorganisms 有权
标题翻译：用于鉴定至少两组微生物的方法
公开(公告)号：US08420345B2
公开(公告)日：2013-04-16
申请号：US12223025
申请日：2007-02-23
申请人： Sylvain Orenga , Celine Roger-Dalbert , Arthur James , John Perry
发明人： Sylvain Orenga , Celine Roger-Dalbert , Arthur James , John Perry
IPC分类号： C12Q1/37 , C12Q1/34 , C12Q1/44 , C12Q1/42
CPC分类号： C12Q1/04 , C12Q1/045
摘要： The present invention relates to a method for identifying at least two groups of microorganisms expressing the same enzymatic activity, comprising the following steps: a) incubating said groups of microorganisms in a reaction medium comprising a first enzyme substrate and a second enzyme substrate, said first and second enzyme substrates being metabolized by the same enzymatic activity; b) identifying said groups of microorganisms.
摘要翻译：本发明涉及用于鉴定表达相同酶活性的至少两组微生物的方法，包括以下步骤：a）将所述微生物组在包含第一酶底物和第二酶底物的反应介质中孵育，所述第一并且第二酶底物被相同的酶活性代谢; b）鉴定所述微生物组。

7. 发明授权

US07691601B2 Medium for detecting and/or identifying of microorganisms 有权
标题翻译：用于检测和/或识别微生物的培养基
公开(公告)号：US07691601B2
公开(公告)日：2010-04-06
申请号：US10552508
申请日：2004-04-02
申请人： Sylvain Orenga , Celine Roger-Dalbert , John Perry , Arthur James
发明人： Sylvain Orenga , Celine Roger-Dalbert , John Perry , Arthur James
IPC分类号： C12Q1/04 , C12Q1/10
CPC分类号： C12Q1/045 , C12Q1/10
摘要： The invention relates to a medium for detecting and/or identifying microorganisms present in a sample, comprising a culture medium and at least one substrate that can be hydrolysed to a labelled product by at least a first enzyme not free in the sample, and specific for the microorganisms, characterized in that it also comprises at least one inhibitor of at least a second enzyme, different from the first enzyme or identical to it, but free in said sample and not originating from a microorganism. The present invention also finds a preferred application in the field of biomedical diagnosis or in food microbiology, and more particularly in bacteriology and mycology.
摘要翻译：本发明涉及一种用于检测和/或鉴定样品中存在的微生物的培养基，其包含培养基和至少一种可通过样品中不含游离的至少第一种酶水解成标记产物的底物，并且特异于所述微生物的特征在于，其还包含至少一种与第一种酶不同的第二种酶的至少一种抑制剂，或与之相同，但在所述样品中游离并且不来自微生物。本发明还发现在生物医学诊断或食品微生物学领域中的优选应用，更特别地在细菌学和真菌学领域。

8. 发明申请

US20080145879A1 Medium for the Specific Detection of Resistant Microorganisms 审中-公开
标题翻译：用于特异性检测抗性微生物的培养基
公开(公告)号：US20080145879A1
公开(公告)日：2008-06-19
申请号：US11794907
申请日：2006-02-09
申请人： Sylvain Orenga , Celine Roger-Dalbert , John Perry , Vanessa Chanteperdrix , Gilles Zambardi , Nathalie Bal
发明人： Sylvain Orenga , Celine Roger-Dalbert , John Perry , Vanessa Chanteperdrix , Gilles Zambardi , Nathalie Bal
IPC分类号： C12Q1/54 , C12Q1/04 , C12M1/34 , C12Q1/20 , C12Q1/10 , C12Q1/42
CPC分类号： C12Q1/34 , C12Q1/04 , C12Q1/045 , C12Q1/10 , C12Q1/14 , C12Q1/37 , C12Q1/527
摘要： The invention relates to the use of a combination of two culture media for distinguishing at least three groups of microorganisms in a biological sample, comprising: a first group of microorganisms, belonging to a first taxon of microorganisms and comprising at least a first mechanism of resistance to a first treatment; a second group of microorganisms, belonging to a second taxon of microorganisms and comprising at least a second mechanism of resistance to a second treatment; a third group of microorganisms, that are not resistant to said first and second treatments, said combination of two culture media comprising: a. at least a first substrate for detecting at least a first enzymatic or metabolic activity of said first group of microorganisms; b. at least two markers for differentiating the first group of microorganisms and the second group of microorganisms; c. at least one antimicrobial that is active on said third group of microorganisms.
摘要翻译：本发明涉及两种培养基的组合用于区分生物样品中的至少三组微生物的组合，其包括：属于微生物的第一分类群的第一组微生物，并且至少包含第一抗性机制第一次治疗; 属于微生物的第二分类单元的第二组微生物，并且包含至少第二种抵抗第二种处理的机制; 第三组微生物，其对所述第一和第二处理不具有抗性，所述两种培养基的组合包括：a。至少第一底物，用于检测所述第一组微生物的至少第一酶促或代谢活性; b。用于分化第一组微生物和第二组微生物的至少两种标记物; C。至少一种在所述第三组微生物上具有活性的抗微生物剂。

9. 发明申请

US20100062466A1 MEDIUM FOR THE DETECTION AND/OR IDENTIFICATION OF BACTERIA 有权
标题翻译：用于检测和/或鉴定细菌的介质
公开(公告)号：US20100062466A1
公开(公告)日：2010-03-11
申请号：US12448851
申请日：2008-02-07
申请人： Marine Casse , Sylvain Orenga , Celine Roger-Dalbert
发明人： Marine Casse , Sylvain Orenga , Celine Roger-Dalbert
IPC分类号： C12Q1/34 , C12N1/20
CPC分类号： C12Q1/04 , C12N1/20 , C12Q1/10 , G01N33/569
摘要： A detection medium including a substrate for a metabolic activity specific for a group of Gram-negative bacteria a beta-glucosidase or cellobiosidase substrate and a beta-glucosidase or cellobiosidase inducer. The inducer is a carbohydrate constituted of a carbohydrate linked in the β-position to glucose, or a carbohydrate with a β glucoside subunit, preferably selected from cellobiose, cellulose, starch, cellotriose and trehalose.
摘要翻译：一种检测介质，其包括用于革兰氏阴性细菌一组β-葡糖苷酶或纤维二糖苷酶底物和β-葡糖苷酶或纤维二糖苷酶诱导物的代谢活性的底物。诱导剂是由与葡萄糖连接的碳水化合物或具有β-葡萄糖的碳水化合物构成的碳水化合物。葡糖苷亚基，优选选自纤维二糖，纤维素，淀粉，纤维三糖和海藻糖。

10. 发明申请

US20050014215A1 Method and medium for the detecting/identifying microorganisms with esterase and/or r osidase and/or peptidase and/or sulfatase and/or phosphatase activity 有权
标题翻译：用酯酶和/或rOSI酶和/或肽酶和/或硫酸酯酶和/或磷酸酶活性检测/鉴定微生物的方法和培养基
公开(公告)号：US20050014215A1
公开(公告)日：2005-01-20
申请号：US10416591
申请日：2001-11-16
申请人： Sandra Gilbert , Celine Roger-Dalbert , Sylvain Orenga
发明人： Sandra Gilbert , Celine Roger-Dalbert , Sylvain Orenga
IPC分类号： C12N1/00 , C12N1/20 , C12Q1/04 , C12Q1/10 , C12Q1/26 , C12Q1/34 , C12Q1/37 , C12Q1/42 , C12Q1/44 , C12R1/01
CPC分类号： C12Q1/44 , C12N1/20 , C12Q1/045 , C12Q1/10 , C12Q1/34 , C12Q1/37 , C12Q1/42 , G01N2333/255 , G01N2496/25
摘要： The invention relates to a medium for biological analysis by biochemical means involving chromogenic or fluorogenic substrates that react with enzymes (esterases and/or osidases and/or peptidases and/or sulfatases and/or phosphatases) specific for the target strains. The object of the invention is to improve the sensitivity, initial translucence, stability and ease of use of such identification/detection media. This object is achieved by the medium according to the invention, which is in a stable, ready-to-use liquid or gel form and contains at least one emulsifying stabilizer of the fatty acid sorbitan ester/fatty acid type and at least one solvent (dimethyl sulfoxide). This medium is obtained by mixing a stock solution of esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase substrates in the solvent, and the emulsifying stabilizer, with some of the constituents of the culture medium. The invention further relates to the preparation of such a medium and to the use of a fatty acid sorbitan ester/fatty acids as an emulsifying stabilizer in a medium for the detection/identification of microorganisms, especially bacteria or yeasts, with esterase and/or osidase and/or peptidase and/or sulfatase and/or phosphatase activities.
摘要翻译：本发明涉及通过生物化学方法进行生物分析的介质，所述生物化学方法涉及与靶标菌株特异的酶（酯酶和/或糖苷酶和/或肽酶和/或硫酸酯酶和/或磷酸酶）反应的显色或荧光底物。本发明的目的是提高这种鉴定/检测介质的灵敏度，初始半透明度，稳定性和易用性。该目的是通过本发明的介质来实现的，该介质是稳定的即用液体或凝胶形式，并含有至少一种脂肪酸脱水山梨醇酯/脂肪酸型乳化稳定剂和至少一种溶剂（二甲基亚砜）。该培养基通过将溶剂中的酯酶和/或糖苷酶和/或肽酶和/或硫酸酯酶和/或磷酸酶底物的储备溶液与乳化稳定剂与培养基的一些成分混合而获得。本发明还涉及这种培养基的制备，以及在用于通过酯酶和/或氧化酶检测/鉴定微生物特别是细菌或酵母的培养基中使用脂肪酸脱水山梨糖醇酯/脂肪酸作为乳化稳定剂和/或肽酶和/或硫酸酯酶和/或磷酸酶活性。

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