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    • 3. 发明申请
    • BACTERIA DETECTION AND/OR IDENTIFICATION MEDIUM
    • 细菌检测和/或鉴定中
    • US20100255530A1
    • 2010-10-07
    • US12448823
    • 2008-02-07
    • Daniel MongetSylvain OrengaMichel PeyretCeline Roger-Dalbert
    • Daniel MongetSylvain OrengaMichel PeyretCeline Roger-Dalbert
    • C12Q1/10
    • C12Q1/045C12Q1/10C12Q1/34C12Q2334/50G01N2333/245
    • The invention relates to a method for detecting and/or identifying E. coli in a urine sample, that comprises: a) inoculating the urine sample liable to contain E. coli on a detection medium that comprises a first substrate selected from a beta-glucuronidase substrate, beta-galactosidase substrate and alpha-galactosidase substrate, and a substrate for a lactose acidification enzyme, beta-ribosidase, phosphatase, L-alanine aminopeptidase and L-leucine aminopeptidase, and a second substrate, different from said first substrate and selected from a beta-glucuronidase substrate, beta-galactosidase substrate and alpha-galactosidase substrate, and a substrate for a lactose acidification enzyme, beta-ribosidase, phosphatase, L-alanine aminopeptidase and L-leucine aminopeptidase, or obtaining bacterial colonies; b) identifying the colonies that react with the first substrate and/or the second substrate as being colonies of E. coli.
    • 本发明涉及一种用于检测和/或鉴定尿液样本中大肠杆菌的方法,其包括:a)将含有大肠杆菌的尿液样本接种在检测培养基上,所述检测培养基包含选自β-葡糖苷酸酶 底物,β-半乳糖苷酶底物和α-半乳糖苷酶底物,以及用于乳糖酸化酶,β-核糖酶,磷酸酶,L-丙氨酸氨基肽酶和L-亮氨酸氨基肽酶的底物,以及与所述第一底物不同的第二底物, β-葡糖醛酸酶底物,β-半乳糖苷酶底物和α-半乳糖苷酶底物,以及用于乳糖酸化酶,β-核糖酶,磷酸酶,L-丙氨酸氨基肽酶和L-亮氨酸氨基肽酶的底物,或获得细菌菌落; b)鉴定作为大肠杆菌菌落与第一底物和/或第二底物反应的菌落。