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1. 发明授权

US09205465B2 High throughput root washing method, apparatus, and system 有权
标题翻译：高通量根洗方法，仪器和系统
公开(公告)号：US09205465B2
公开(公告)日：2015-12-08
申请号：US13154141
申请日：2011-06-06
申请人： Daniel M. Goldman , Xiaomu Niu
发明人： Daniel M. Goldman , Xiaomu Niu
IPC分类号： B08B3/02 , B08B3/14 , B08B3/04 , B08B3/00 , A01G7/00
CPC分类号： B08B3/024 , A01G7/00 , B08B3/00 , B08B3/02 , B08B3/04 , B08B3/14
摘要： The present invention provides a method, apparatus, and system for efficient, high throughput, consistent cleaning of soil from the root system of a plant. In one embodiment, the method generally comprises positioning a plant adjacent a sprayer assembly having at least one nozzle angled so as to impact the root system at a predetermined position, providing a stream of water from a reservoir through the nozzle of the sprayer assembly, and rotating at least one of the root system and the nozzle relative to the other to remove the soil from the root system. The present invention further describes a recycling system adapted to recycle water expended during the cleaning process to minimize losses and to capture and return dislodged soil to the field from which the plant is extracted.
摘要翻译：本发明提供了一种用于从植物根系中有效，高通量，一致地清洁土壤的方法，装置和系统。在一个实施例中，该方法通常包括将具有至少一个喷嘴的喷雾器组件相邻的设备定位成角度以便在预定位置冲击根系，从储存器通过喷雾器组件的喷嘴提供水流;以及使根系和喷嘴中的至少一个相对于另一个旋转，以从根系中去除土壤。本发明进一步描述了一种循环系统，其适于循环在清洁过程中消耗的水以使损失最小化并捕获并返回移出的土壤到植物被提取的田地。

2. 发明申请

US20110297191A1 HIGH THROUGHPUT ROOT WASHING METHOD, APPARATUS, AND SYSTEM 有权
标题翻译：高通量洗涤方法，装置和系统
公开(公告)号：US20110297191A1
公开(公告)日：2011-12-08
申请号：US13154141
申请日：2011-06-06
申请人： Daniel M. Goldman , Xiaomu Niu
发明人： Daniel M. Goldman , Xiaomu Niu
IPC分类号： B08B3/02
CPC分类号： B08B3/024 , A01G7/00 , B08B3/00 , B08B3/02 , B08B3/04 , B08B3/14
摘要： The present invention provides a method, apparatus, and system for efficient, high throughput, consistent cleaning of soil from the root system of a plant. In one embodiment, the method generally comprises positioning a plant adjacent a sprayer assembly having at least one nozzle angled so as to impact the root system at a predetermined position, providing a stream of water from a reservoir through the nozzle of the sprayer assembly, and rotating at least one of the root system and the nozzle relative to the other to remove the soil from the root system. The present invention further describes a recycling system adapted to recycle water expended during the cleaning process to minimize losses and to capture and return dislodged soil to the field from which the plant is extracted.
摘要翻译：本发明提供了一种用于从植物根系中有效，高通量，一致地清洁土壤的方法，装置和系统。在一个实施例中，该方法通常包括将具有至少一个喷嘴的喷雾器组件相邻的设备定位成角度以便在预定位置冲击根系，从储存器通过喷雾器组件的喷嘴提供水流;以及使根系和喷嘴中的至少一个相对于另一个旋转，以从根系中去除土壤。本发明进一步描述了一种循环系统，其适于循环在清洁过程中消耗的水以使损失最小化并捕获并返回移出的土壤到植物被提取的田地。

3. 发明授权

US09677084B2 Down-regulation of a homeodomain-leucine zipper I-class homeobox gene for improved plant performance 有权
公开(公告)号：US09677084B2
公开(公告)日：2017-06-13
申请号：US13453020
申请日：2012-04-23
申请人： Mei Guo , Xiaomu Niu , Mary Rupe , Jeffrey Schussler
发明人： Mei Guo , Xiaomu Niu , Mary Rupe , Jeffrey Schussler
IPC分类号： C12N15/113 , C12N15/82 , C07K14/415
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/113 , C12N15/8282 , Y02A40/146
摘要： Methods for modulating plants using optimized ZmME293 down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear number and/or reduction in time to scenescence.

4. 发明申请

US20120278947A1 DOWN-REGULATION OF A HOMEODOMAIN-LEUCINE ZIPPER I-CLASS HOMEOBOX GENE FOR IMPROVED PLANT PERFORMANCE 有权
标题翻译：家庭用品拉链器一级家禽基因的下调调节植物性能
公开(公告)号：US20120278947A1
公开(公告)日：2012-11-01
申请号：US13453020
申请日：2012-04-23
申请人： Mei Guo , Xiaomu Niu , Mary Rupe , Jeffrey R. Schussler
发明人： Mei Guo , Xiaomu Niu , Mary Rupe , Jeffrey R. Schussler
IPC分类号： C12N15/82 , C12N5/10 , A01H5/10 , C07H21/04
CPC分类号： C12N15/8261 , C07K14/415 , C12N15/113 , C12N15/8282 , Y02A40/146
摘要： Methods for modulating plants using optimized ZmME293 down-regulation constructs are disclosed. Also disclosed are nucleotide sequences, constructs, vectors, and modified plant cells, as well as transgenic plants displaying increased seed and/or biomass yield, improved tolerance to abiotic stress such as drought or high plant density, improved nitrogen utilization efficiency, increased ear number and/or reduction in time to scenescence.
摘要翻译：公开了使用优化的ZmME293下调构建体调节植物的方法。还公开了核苷酸序列，构建体，载体和修饰的植物细胞，以及显示增加的种子和/或生物量产量的转基因植物，对非生物胁迫如干旱或高植物密度的改善的耐受性，改善的氮利用效率，增加的耳数和/或减少到场景的时间。

5. 发明申请

US20120124697A1 PLANTS WITH ALTERED ROOT ARCHITECTURE, RELATED CONSTRUCTS AND METHODS INVOLVING GENES ENCODING NUCLEOSIDE DIPHOSPHATASE KINASE (NDK) POLYPEPTIDES AND HOMOLOGS THEREOF 审中-公开
标题翻译：具有改变的根结构的植物，涉及编码核苷酸二磷酸酶激酶（NDK）多肽的基因的相关构建体和方法及其同源物
公开(公告)号：US20120124697A1
公开(公告)日：2012-05-17
申请号：US12671808
申请日：2008-08-29
申请人： Graziana Taramino , Scott V. Tingey , Stephen M. Allen , Dwight Tomes , Stanley Luck , Hajime Sakai , Xiaomu Niu
发明人： Graziana Taramino , Scott V. Tingey , Stephen M. Allen , Dwight Tomes , Stanley Luck , Hajime Sakai , Xiaomu Niu
IPC分类号： A01H5/00 , C12N15/82 , C12N15/54 , A01H5/10
CPC分类号： C12N15/8273 , C12N9/1229 , C12N15/8262
摘要： Isolated polynucleotides and polypeptides and recombinant DNA constructs particularly useful for altering root structure of plants, compositions (such as plants or seeds) comprising these recombinant DNA constructs, and methods utilizing these recombinant DNA constructs. The recombinant DNA construct comprises a polynucleotide operably linked to a promoter functional in a plant, wherein said polynucleotide encodes a polypeptide useful for altering plant root architecture.
摘要翻译：分离的多核苷酸和多肽以及特别可用于改变植物的根结构，包含这些重组DNA构建体的组合物（例如植物或种子）以及利用这些重组DNA构建体的方法的重组DNA构建体。重组DNA构建体包含可操作地连接到在植物中起作用的启动子的多核苷酸，其中所述多核苷酸编码可用于改变植物根结构的多肽。

6. 发明授权

US08049000B2 Early endosperm promoter eep2 失效
标题翻译：早期胚乳启动子eep2
公开(公告)号：US08049000B2
公开(公告)日：2011-11-01
申请号：US12463450
申请日：2009-05-11
申请人： Timothy G Helentjaris , Xiaomu Niu
发明人： Timothy G Helentjaris , Xiaomu Niu
IPC分类号： C12N15/29
CPC分类号： C12N15/8234 , C12N9/0032 , C12N9/1085 , C12N15/8243 , C12N15/8261 , C12N15/8271 , C12N15/8295 , Y02A40/146
摘要： This invention relates generally to the field of plant molecular biology. More specifically, this invention relates to methods and reagents for the temporally- and/or spatially-regulated expression of genes, particularly in plant seeds and related female reproductive tissue. Compositions comprise novel nucleotide sequences for a seed-preferred promoter known as eep2. A method for expressing a heterologous nucleotide sequence in a plant using the promoter sequences disclosed herein is provided. The method comprises transforming a plant cell to comprise a heterologous nucleotide sequence operably linked to one of the promoters of the present invention and regenerating a stably transformed plant from the transformed plant cell.
摘要翻译：本发明一般涉及植物分子生物学领域。更具体地，本发明涉及用于时间和/或空间调节基因表达的方法和试剂，特别是在植物种子和相关的雌性生殖组织中。组合物包含称为eep2的种子优选启动子的新型核苷酸序列。提供了使用本文公开的启动子序列在植物中表达异源核苷酸序列的方法。该方法包括转化植物细胞以包含与本发明的启动子之一可操作地连接的异源核苷酸序列，并从转化的植物细胞再生稳定转化的植物。

7. 发明授权

US08044263B2 Cytokinin oxidase promoter from maize 失效
标题翻译：来自玉米的细胞分裂素氧化酶启动子
公开(公告)号：US08044263B2
公开(公告)日：2011-10-25
申请号：US12819888
申请日：2010-06-21
申请人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugière
发明人： Xiaomu Niu , Haiyin Wang , Dwight T. Tomes , Sabine Hantke , Norbert Brugière
IPC分类号： C12N15/82 , A01H5/00 , A01H1/00
CPC分类号： C07K14/415 , C12N9/0032 , C12N15/8223 , C12N15/8295
摘要： The present invention provides compositions and methods for regulating expression of nucleotide sequences in a plant. Compositions and methods include expression cassettes and transformed plants and provide for downregulation of cytokinin oxidase in a plant.
摘要翻译：本发明提供调节植物中核苷酸序列表达的组合物和方法。组合物和方法包括表达盒和转化植物，并提供植物中细胞分裂素氧化酶的下调。

8. 发明申请

US20070044176A1 Nitrate transport components 有权
标题翻译：硝酸盐运输组件
公开(公告)号：US20070044176A1
公开(公告)日：2007-02-22
申请号：US11504116
申请日：2006-08-15
申请人： Stephen Allen , Kanwarpal Dhugga , Kevin Fengler , Howard Hershey , Victor Llaca , Dale Loussaert , Lu Liu , Xiaomu Niu , Haiyin Wang
发明人： Stephen Allen , Kanwarpal Dhugga , Kevin Fengler , Howard Hershey , Victor Llaca , Dale Loussaert , Lu Liu , Xiaomu Niu , Haiyin Wang
IPC分类号： A01H5/00 , C12Q1/68 , C07H21/04 , C12N15/82 , C07K14/415 , C12N5/04
CPC分类号： C12N15/8243 , C07K14/415 , C12N15/8227 , C12N15/8238 , C12N15/8261 , Y02A40/146
摘要： This invention relates to isolated nucleic acid fragments encoding high affinity nitrate transport components. The invention also relates to the construction of recombinant DNA constructs encoding all or a portion of nitrate transport components, in sense or antisense orientation, wherein expression of the recombinant DNA construct may alter levels of the nitrate transport components in a transformed host cell.
摘要翻译：本发明涉及编码高亲和力硝酸盐转运组分的分离的核酸片段。本发明还涉及在正义或反义方向上构建编码全部或部分硝酸盐转运组分的重组DNA构建体，其中重组DNA构建体的表达可改变转化的宿主细胞中硝酸盐转运组分的水平。

9. 发明申请

US20050204432A1 Br2 and Dw3 promoters and methods of use 有权
标题翻译： Br2和Dw3启动子和使用方法
公开(公告)号：US20050204432A1
公开(公告)日：2005-09-15
申请号：US10931077
申请日：2004-08-31
申请人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
发明人： Dilbag Multani , Xiaomu Niu , Dwight Tomes , Haiyin Wang , Deping Xu
IPC分类号： A01H5/00 , C12N15/82
CPC分类号： C07K14/415 , C12N15/8222 , C12N15/8223
摘要： The invention relates to the regulation of gene expression, particularly to methods and compositions for regulating gene expression in plants. Expression cassettes comprising a polynucleotide molecule of interest operably linked to the Brachytic2 (Br2) promoter, the Dwarf3 (Dw3) promoter, or a functional fragment or variant thereof, are provided. Methods of using such expression cassettes, as well as plant cells, plant tissues, plants, and seeds transformed with these expression cassettes, are additionally provided. The methods and compositions find use in regulating the expression of a polynucleotide molecule of interest in a plant.
摘要翻译：本发明涉及基因表达的调节，特别涉及用于调节植物中基因表达的方法和组合物。提供了包含与Brachytic2（Br2）启动子，Dw3（Dw3）启动子或其功能片段或变体可操作连接的目的多核苷酸分子的表达盒。另外提供了使用这些表达盒以及用这些表达盒转化的植物细胞，植物组织，植物和种子的方法。所述方法和组合物用于调节植物中感兴趣的多核苷酸分子的表达。

10. 发明授权

US08173867B2 Methods and compositions for modulating flowering and maturity in plants 有权
标题翻译：用于调节植物开花和成熟的方法和组合物
公开(公告)号：US08173867B2
公开(公告)日：2012-05-08
申请号：US12276961
申请日：2008-11-24
申请人： Dwight T. Tomes , Silvio Salvi , Michele Morgante , Giorgio Sponza , Edward Bruggemann , Xiaomu Niu , Bailin Li , Roberto Tuberosa
发明人： Dwight T. Tomes , Silvio Salvi , Michele Morgante , Giorgio Sponza , Edward Bruggemann , Xiaomu Niu , Bailin Li , Roberto Tuberosa
IPC分类号： C12N15/82
CPC分类号： C12N15/827 , C07K14/415
摘要： The present invention provides compositions and methods for modulating flowering time in plants. Maize RAP2.7 nucleotide sequences are disclosed which upon overexpression cause later flowering and when inhibited cause earlier flowering. Also disclosed is a DNA sequence which acts as a regulator/enhancer of RAP2.7, termed VGT1. This sequence does not code for any known protein, but acts as either a RNAi element or a regulatory DNA or RNA element that either directly regulates expression of flowering genes such as Rap2.7 or specifically targets expression of other genes which control flowering genes such as Rap2.7. This element this can be used as a sequence-based marker to identify inbred and hybrids which have altered maturity. Methods for expressing these nucleotide sequences in a plant for modifying maturity and flowering in plants are provided as well as expression constructs, vectors, transformed cells and plants.
摘要翻译：本发明提供调节植物开花时间的组合物和方法。公开了玉米RAP2.7核苷酸序列，其在过表达导致后期开花并且当被抑制时引起早期开花。还公开了作为RAP2.7调节/增强子的DNA序列，称为VGT1。该序列不编码任何已知的蛋白质，而是作为直接调节开花基因如Rap2.7的表达的RNAi元件或调节DNA或RNA元件，或者特异性靶向控制开花基因的其他基因的表达，例如 Rap2.7。这个元素可以用作基于序列的标记来鉴定已经改变成熟度的近交系和杂交种。提供用于在植物中表达这些核苷酸序列以修饰植物中的成熟和开花的方法以及表达构建体，载体，转化的细胞和植物。

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