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    • 3. 发明申请
    • Method for Optimization of Transgenic Efficacy Using Favorable Allele Variants
    • 使用有利等位基因变体优化转基因功效的方法
    • US20100162440A1
    • 2010-06-24
    • US12641825
    • 2009-12-18
    • Mei GuoMary Rupe
    • Mei GuoMary Rupe
    • A01H5/00C12N15/82C12N5/10
    • C12N15/8261C12N15/8216Y02A40/146
    • The present invention includes methods for using favorable functional allele variants to optimize transgene effects and achieve the maximum potential of transgene efficacy. The present invention provides heterozygous allelic combinations of the transgene by stacking alleles from different heterotic pools, methods to increase crop yield by driving genes (maize or other species) by using heterozygous promoter allele combinations that consist of differentially regulatory allelic elements from heterotic pools, methods to increase crop yield utilizing transgenic complementary paired alleles controlling plant growth and yield. Plants, plant progeny, seeds and tissues created by these methods are also described. Polynucleotides encoding the alleles are provided for expression in a plant of interest. Expression cassettes, plants, plant cells, plant parts and seeds comprising the sequences of the invention are further provided. In specific embodiments, the polynucleotide is operably linked to a native promoter and a transcriptional enhancer.
    • 本发明包括使用有利的功能性等位基因变体来优化转基因作用并达到转基因功效的最大潜力的方法。 本发明通过堆叠来自不同杂种优势池的等位基因来提供转基因的杂合等位基因组合,通过使用由异源池的差异调节等位基因元件组成的杂合启动子等位基因组合来驱动基因(玉米或其他物种)来增加作物产量的方法,方法 使用控制植物生长和产量的转基因互补配对等位基因来提高作物产量。 还描述了通过这些方法产生的植物,植物后代,种子和组织。 提供编码等位基因的多核苷酸用于在感兴趣的植物中表达。 还提供了包含本发明序列的表达盒,植物,植物细胞,植物部分和种子。 在具体实施方案中,多核苷酸可操作地连接到天然启动子和转录增强子。