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1. 发明授权

US07700832B2 Isolated nucleic acids encoding antipathogenic polypeptides and uses thereof 有权
标题翻译：编码抗病原体多肽的分离的核酸及其用途
公开(公告)号：US07700832B2
公开(公告)日：2010-04-20
申请号：US12265461
申请日：2008-11-05
申请人： Daniel J. Altier , Virginia C. Crane , Irina Ellanskaya , Natalia Ellanskaya, legal representative , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric J. Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Virginia C. Crane , Irina Ellanskaya , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric J. Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： C12N15/09 , C12N15/82 , A01H5/00 , A01H5/10
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。也提供了编码抗病原多肽的核酸。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

2. 发明授权

US07598346B1 Antifungal polypeptides 有权
标题翻译：抗真菌多肽
公开(公告)号：US07598346B1
公开(公告)日：2009-10-06
申请号：US11748994
申请日：2007-05-15
申请人： Daniel J. Altier , Virginia C. Crane , Irina Ellanskaya , Natalia Ellanskaya, legal representative , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric J. Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
发明人： Daniel J. Altier , Virginia C. Crane , Irina Ellanskaya , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric J. Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani
IPC分类号： C07K14/385 , A01N65/00 , C12N5/04
CPC分类号： C12N15/8282
摘要： The invention relates to compositions including amino acid sequences isolated from fungal fermentation broths and methods for protecting a plant from a pathogen, particularly a fungal pathogen.
摘要翻译：本发明涉及包含从真菌发酵肉汤中分离的氨基酸序列的组合物和用于保护植物免受病原体，特别是真菌病原体的方法。

3. 发明申请

US20090089895A1 ANTIFUNGAL POLYPEPTIDES 有权
标题翻译：抗菌多糖
公开(公告)号：US20090089895A1
公开(公告)日：2009-04-02
申请号：US12265461
申请日：2008-11-05
申请人： Daniel J. Altier , Virginia C. Crane , Irina Ellanskaya , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric J. Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani , Natalia Ellanskaya
发明人： Daniel J. Altier , Virginia C. Crane , Irina Ellanskaya , Jacob T. Gilliam , Jennie Hunter-Cevera , James K. Presnail , Eric J. Schepers , Carl R. Simmons , Tamas Torok , Nasser Yalpani , Natalia Ellanskaya
IPC分类号： A01H1/00 , C07H21/00 , C12N15/63 , C12N5/04 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for antipathogenic polypeptides that were isolated from fungal fermentation broths. Nucleic acids that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the nucleotide sequences disclosed herein is further provided. The method comprises introducing into a plant an expression cassette comprising a promoter operably linked to a nucleotide sequence that encodes an antipathogenic polypeptide of the invention. Compositions comprising an antipathogenic polypeptide or a transformed microorganism comprising a nucleic acid of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Transformed plants, plant cells, seeds, and microorganisms comprising a nucleotide sequence that encodes an antipathogenic polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括从真菌发酵液中分离的抗病原体多肽的氨基酸序列及其变体和片段。也提供了编码抗病原多肽的核酸。还提供使用本文公开的核苷酸序列在植物中诱导病原体抗性的方法。该方法包括向植物中引入包含可操作地连接到编码本发明的抗病原体多肽的核苷酸序列的启动子的表达盒。还提供包含抗病原体多肽或包含本发明的核酸与载体组合的转化微生物的组合物，以及使用这些组合物保护植物免受病原体的方法。还公开了包含编码本发明的抗病原体多肽的核苷酸序列的转化植物，植物细胞，种子和微生物。

4. 发明授权

US08802932B2 Antipathogenic proteins and methods of use 有权
标题翻译：抗病原性蛋白质及其使用方法
公开(公告)号：US08802932B2
公开(公告)日：2014-08-12
申请号：US13205731
申请日：2011-08-09
申请人： Daniel J. Altier , Jacob T. Gilliam , Eric J. Schepers , Nasser Yalpani
发明人： Daniel J. Altier , Jacob T. Gilliam , Eric J. Schepers , Nasser Yalpani
IPC分类号： C12N15/00 , A01N63/00 , C12N15/82 , C12N15/87 , A01H5/00 , A01H5/10
CPC分类号： C07K14/37 , A01N63/04 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for family members of a novel family of antipathogenic polypeptides. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polnucleotide or polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括氨基酸序列及其变体和片段，用于新型抗病原体多肽家族的家族成员。也提供了编码抗病原多肽的多核苷酸。还提供使用本文公开的多核苷酸在植物中诱导病原体抗性的方法。包含抗病原体多肽或包含本发明的抗病原体多核苷酸与载体组合的组合物以及使用这些组合物保护植物免受病原体的方法。还公开了包含本发明的抗病原性核苷酸或多肽的植物，植物细胞，种子和微生物。

5. 发明申请

US20120054910A1 Antipathogenic Proteins and Methods of Use 有权
标题翻译：抗病原体蛋白和使用方法
公开(公告)号：US20120054910A1
公开(公告)日：2012-03-01
申请号：US13205731
申请日：2011-08-09
申请人： Daniel J. Altier , Jacob T. Gilliam , Eric J. Schepers , Nasser Yalpani
发明人： Daniel J. Altier , Jacob T. Gilliam , Eric J. Schepers , Nasser Yalpani
IPC分类号： A01H1/00 , C07K14/37 , A61K38/16 , A01H5/10 , C12N1/21 , C12N1/19 , C12N5/10 , A01H5/00 , C12N15/31 , C12N1/00
CPC分类号： C07K14/37 , A01N63/04 , C12N15/8282
摘要： Compositions and methods for protecting a plant from a pathogen, particularly a fungal pathogen, are provided. Compositions include amino acid sequences, and variants and fragments thereof, for family members of a novel family of antipathogenic polypeptides. Polynucleotides that encode the antipathogenic polypeptides are also provided. A method for inducing pathogen resistance in a plant using the polynucleotides disclosed herein is further provided. Compositions comprising an antipathogenic polypeptide or a microorganism comprising an antipathogenic polynucleotide of the invention in combination with a carrier and methods of using these compositions to protect a plant from a pathogen are further provided. Plants, plant cells, seeds, and microorganisms comprising an antipathogenic polnucleotide or polypeptide of the invention are also disclosed.
摘要翻译：提供了用于保护植物免受病原体，特别是真菌病原体的组合物和方法。组合物包括氨基酸序列及其变体和片段，用于新型抗病原体多肽家族的家族成员。也提供了编码抗病原多肽的多核苷酸。还提供使用本文公开的多核苷酸在植物中诱导病原体抗性的方法。包含抗病原体多肽或包含本发明的抗病原体多核苷酸与载体组合的组合物以及使用这些组合物保护植物免受病原体的方法。还公开了包含本发明的抗病原性核苷酸或多肽的植物，植物细胞，种子和微生物。

6. 发明授权

US07462760B2 Genes encoding plant protease-resistant pesticidal proteins and method of their use 失效
标题翻译：编码植物蛋白酶抗性杀虫蛋白的基因及其使用方法
公开(公告)号：US07462760B2
公开(公告)日：2008-12-09
申请号：US10746914
申请日：2003-12-24
申请人： André Abad , Hua Dong , Rafael Herrmann , Albert Lu , Billy F. McCutchen , Janet A. Rice , Eric J. Schepers , James F. Wong
发明人： André Abad , Hua Dong , Rafael Herrmann , Albert Lu , Billy F. McCutchen , Janet A. Rice , Eric J. Schepers , James F. Wong
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C07K14/325 , C12N15/8286 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. The invention provides mutagenized nucleic acids that have been engineered to encode pesticidal polypeptides having increased resistance to proteolytic degradation by a plant protease. In particular, nucleic acid sequences encoding pesticidal polypeptides modified to comprise a proteolytic protection site that confers resistance to degradation or proteolytic inactivation by a plant protease are provided. Particular embodiments of the invention provide expression cassettes and transformed plants, plant cells, and seeds. These compositions find use in methods for protecting a plant from a pest.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。本发明提供已被工程化以编码具有增加的抗植物蛋白酶对蛋白水解降解的杀虫多肽的诱变的核酸。特别地，提供了编码杀虫多肽的核酸序列，其被修饰为包含赋予植物蛋白酶降解或蛋白水解灭活抗性的蛋白水解保护位点。本发明的具体实施方案提供表达盒和转化植物，植物细胞和种子。这些组合物用于保护植物免受害虫的方法。

7. 发明授权

US07473821B2 Nucleic acids encoding Cry8Bb1 endotoxins engineered to have insect-specific protease recognition sequences 有权
标题翻译：编码Cry8Bb1内毒素的核酸被改造为具有昆虫特异性蛋白酶识别序列
公开(公告)号：US07473821B2
公开(公告)日：2009-01-06
申请号：US11448266
申请日：2006-06-07
申请人： Andre R. Abad , Gary C. Chun , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Janet A. Rice , Eric J. Schepers
发明人： Andre R. Abad , Gary C. Chun , Ronald D. Flannagan , Rafael Herrmann , Albert L. Lu , Janet A. Rice , Eric J. Schepers
IPC分类号： A01H5/00 , A01H5/10 , C12N15/82 , C12N15/32
CPC分类号： C12N15/8286 , A01N63/02 , C07K14/325 , C12N9/6472 , Y02A40/162
摘要： Compositions and methods for protecting a plant from an insect pest are provided. Nucleic acid molecules encoding insect protoxins or insect toxins modified to comprise at least one proteolytic activation site that is sensitive to an insect gut protease are provided. Cleavage of a modified insect protoxin at the proteolytic activation site by an insect gut protease produces an active insect toxin in the gut of the insect pest. Cleavage of a modified insect toxin of the invention at a proteolytic activation site results in the production of an active insect toxin in the insect gut that displays improved pesticidal activity relative to the insect toxin that lacks the proteolytic activation site. Methods of using the modified insect protoxin and modified insect toxin nucleic acid sequences and the polypeptides they encode to protect a plant from an insect pest are provided. Particular embodiments of the invention further provide modified insect protoxin and modified insect toxin compositions and formulations, expression cassettes, and transformed plants, plant cells, and seeds. Insect gut proteases and the nucleic acid molecules that encode them are also disclosed herein.
摘要翻译：提供了保护植物免受害虫害虫的组合物和方法。提供编码昆虫原毒素或昆虫毒素的核酸分子，其被修饰为包含至少一个对昆虫肠道蛋白酶敏感的蛋白水解活化位点。通过昆虫肠蛋白酶在蛋白水解活化位点切割修饰的昆虫原毒素在害虫的肠道中产生活性昆虫毒素。在蛋白水解活化位点切割本发明的修饰的昆虫毒素导致在昆虫肠中产生活性昆虫毒素，其相对于缺乏蛋白水解活化位点的昆虫毒素显示出改善的杀虫活性。提供了使用修饰的昆虫原毒素和修饰的昆虫毒素核酸序列及其编码的多肽以保护植物免受害虫的方法。本发明的具体实施方案还提供改性的昆虫原毒素和修饰的昆虫毒素组合物和制剂，表达盒，以及转化的植物，植物细胞和种子。昆虫肠道蛋白酶和编码它们的核酸分子也在本文中公开。

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