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1. 发明申请

US20220250073A1 INTEGRATED AIR FILTERING AND CONDITIONING OF DROPLET CHAMBER IN A COMPACT CELL SORTER 有权
公开(公告)号：US20220250073A1
公开(公告)日：2022-08-11
申请号：US17665482
申请日：2022-02-04
申请人： CYTEK BIOSCIENCES, INC.
发明人： Glen Krueger , David Vrane , Kuncheng Wang , Qiuta Gu
IPC分类号： B01L3/00 , G01N15/14
摘要： A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment.
A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.

2. 发明公开

US20240278240A1 METHODS FOR CELL SORTERS WITH PIVOTAL SCUPPERS 审中-公开
公开(公告)号：US20240278240A1
公开(公告)日：2024-08-22
申请号：US18632183
申请日：2024-04-10
申请人： Cytek Biosciences, Inc.
发明人： Glen Krueger , David Vrane , Kuncheng Wang , Qiuta Gu
IPC分类号： B01L3/00 , C12M1/00 , G01N15/01 , G01N15/10 , G01N15/14 , G01N15/1409 , G01N15/1434 , G01N15/149
CPC分类号： B01L3/502761 , B01L3/502715 , C12M47/04 , G01N15/14 , G01N15/1436 , B01L2300/0654 , B01L2300/0663 , B01L2300/0681 , B01L2300/0829 , B01L2300/1805 , B01L2300/1894 , G01N15/01 , G01N2015/1006 , G01N2015/1028 , G01N15/1409 , G01N15/149
摘要： Methods for a sorting flow cytometer system are disclosed. The method includes forming a stream of a sample biological fluid; charging the stream of the sample biological fluid with a first voltage having a first voltage polarity; breaking a first charged drop off of the stream, wherein the first charged drop is charged with the first voltage; dropping the first charged drop between a first electrostatic charge plate and a second electrostatic charge plate opposite the first electrostatic charge plate, wherein the first electrostatic charge plate and the second electrostatic charge plate are positioned on an angle to form a progressively larger gap between each; deflecting the first charged drop off of a center stream path towards the first electrostatic charge plate to drop along a line with a first angle; and orienting a first pivotal scupper to allow the first charged drop to fall into a first test tube.

3. 发明申请

US20220371017A1 CELL SORTER WITH PIVOTAL SCUPPERS 有权
公开(公告)号：US20220371017A1
公开(公告)日：2022-11-24
申请号：US17829321
申请日：2022-05-31
申请人： CYTEK BIOSCIENCES, INC.
发明人： Glen Krueger , David Vrane , Kuncheng Wang , Qiuta Gu
IPC分类号： B01L3/00 , G01N15/14
摘要： A compact sorting flow cytometer system is disclosed. The system includes a fluidics system having a flow cell and a deflection chamber in communication with the flow cell to receive drops in a stream of a sample biological fluid with one or more biological cells or particles and selectively deflect the drops in the stream of the sample biological fluid with the one or more biological cells or particles; and a droplet deposition unit (DDU) system in communication with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid with the one or more biological cells or particles into one or more containers. The DDU system includes a case or a housing with an open face surround by edges of the case, the case forming a portion of a containment chamber, the case having a top side opening aligned with the deflection chamber to receive the selectively deflected drops in the stream of the sample biological fluid into one or more containers in the containment chamber, a seal mounted around edges of the case, one or more hinges coupled to a bottom portion of the case, and a door coupled to the one or more hinges to pivot the door about the one or more hinges, the door when closed to press against the seal and close off the containment chamber from an external environment.
A method for evacuation of air in a containment chamber of a flow cytometer is disclosed. The method includes turning off a return fan in a first tunnel between an air conditioning chamber and a containment chamber; turning on an evacuation fan in a second tunnel between the air conditioning chamber and the containment chamber, the evacuation fan pulling air out of the containment chamber into the air conditioning chamber, opening a valve in an evacuation vent, the evacuation fan pushing air out of the air conditioning chamber through the evacuation vent into the environment; and continuously running the evacuation fan for a predetermined period of time to evacuate air out of the containment chamber.

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