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1. 发明申请

US20080248589A1 Sample Presentation Device 审中-公开
标题翻译：样品展示装置
公开(公告)号：US20080248589A1
公开(公告)日：2008-10-09
申请号：US11596285
申请日：2005-05-19
申请人： Christopher M. Belisle , John A. Walker , Sarah M. Ngola , Douglas P. Greiner , Mark J. Levy , Xiaoxia Zhao , Irene Y. Chen , Mark L. Stolowitz , Donald P. Paquin
发明人： Christopher M. Belisle , John A. Walker , Sarah M. Ngola , Douglas P. Greiner , Mark J. Levy , Xiaoxia Zhao , Irene Y. Chen , Mark L. Stolowitz , Donald P. Paquin
IPC分类号： G01N33/543 , B01J19/00
CPC分类号： B01L3/5085 , B01L3/502707 , B01L3/502792 , B01L3/5088 , B01L2200/12 , B01L2300/087 , B01L2300/089 , B82Y15/00 , B82Y30/00 , C23C26/00 , C23C30/00 , Y10T436/24 , Y10T436/25
摘要： The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.
摘要翻译：本发明涉及可用于执行分析测量的样本呈现装置。这些装置已被配置成能够实现液体处理的各个方面，例如：保留，储存，运输，集中，定位和转移。此外，这些设备可以增强分析物的检测和表征。本发明的样品展示装置由具有不同润湿性的多个区域的一个或多个基材组成。公开了使用本发明的样品呈现装置分析样品的方法以及制备样品显示装置的方法。

2. 发明申请

US20110070659A1 SAMPLE PRESENTATION DEVICE 审中-公开
标题翻译：样品演示装置
公开(公告)号：US20110070659A1
公开(公告)日：2011-03-24
申请号：US12786133
申请日：2010-05-24
申请人： Christopher M. Belisle , John A. Walker, II , Sarah M. Ngola , Douglas P. Greiner , Mark J. Levy , Xiaoxia Zhao , Irene Y. Chen , Mark L. Stolowitz , Donald P. Paquin
发明人： Christopher M. Belisle , John A. Walker, II , Sarah M. Ngola , Douglas P. Greiner , Mark J. Levy , Xiaoxia Zhao , Irene Y. Chen , Mark L. Stolowitz , Donald P. Paquin
IPC分类号： G01N33/543 , G01N1/00 , G01N33/50 , B01L9/00 , G01N21/00 , G01N21/76 , G01N24/00 , G01N30/96
CPC分类号： B01L3/5085 , B01L3/502707 , B01L3/502792 , B01L3/5088 , B01L2200/12 , B01L2300/087 , B01L2300/089 , B82Y15/00 , B82Y30/00 , C23C26/00 , C23C30/00 , Y10T436/24 , Y10T436/25
摘要： The present invention relates to sample presentation devices useful in performing analytical measurements. These devices have been configured to enable various aspects of liquid handling such as: retention, storage, transport, concentration, positioning, and transfer. Additionally, these devices can enhance the detection and characterization of analytes. The sample presentation devices of the present invention are comprised of one or more substrates having a plurality of zones of differing wettability. Methods of analyzing samples using the sample presentation device of the invention, as well as methods of making the sample presentation devices are disclosed.
摘要翻译：本发明涉及可用于执行分析测量的样本呈现装置。这些装置已被配置成能够实现液体处理的各个方面，例如：保留，储存，运输，集中，定位和转移。此外，这些设备可以增强分析物的检测和表征。本发明的样品展示装置由具有不同润湿性的多个区域的一个或多个基材组成。公开了使用本发明的样品呈现装置分析样品的方法，以及制备样品显示装置的方法。

3. 发明授权

US06818760B1 Removal of dye-labeled dideoxy terminators from DNA sequencing reactions 失效
标题翻译：从DNA测序反应中去除染料标记的双脱氧终止子
公开(公告)号：US06818760B1
公开(公告)日：2004-11-16
申请号：US09680889
申请日：2000-10-06
申请人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
发明人： Douglas A. Spicer , Karin A. Hughes , Robert J. Kaiser , James E. Mahoney , Amy L. Springer , Mark L. Stolowitz , Carl H. D. Weissman
IPC分类号： C07H2102
CPC分类号： C12Q1/6869 , C12Q1/6844 , C12Q2563/107 , C12Q2563/149 , C12Q2565/137
摘要： This invention provides methods for removing unincorporated dye-labeled molecules from a mixture that includes dye-labeled polynucleotides or other polymers and the unincorporated dye-labeled molecules. The methods involve adsorbing the unincorporated dye-labeled molecules into a plurality of particles that are made up of one or more porous hydrophobic materials that are encapsulated in a hydrophilic matrix.
摘要翻译：本发明提供了从包含染料标记的多核苷酸或其它聚合物的混合物和未掺入的染料标记分子的混合物中除去未结合的染料标记的分子的方法。所述方法包括将未结合的染料标记的分子吸附到多个由包封在亲水基质中的一种或多种多孔疏水性材料构成的颗粒中。

4. 发明授权

US6031117A Boronic acid containing phosphoramidite reagents 失效
标题翻译：含有亚磷酰胺试剂的硼酸
公开(公告)号：US6031117A
公开(公告)日：2000-02-29
申请号：US272978
申请日：1999-03-19
申请人： Robert J. Kaiser , Mark L. Stolowitz
发明人： Robert J. Kaiser , Mark L. Stolowitz
IPC分类号： C07F9/6596 , C07H21/00 , C07F5/04
CPC分类号： C07H21/00 , C07F9/6596
摘要： The present invention provides arylboronic acid reagents useful for incorporation into modified oligonucleotides and polynucleotides. The modified oligonucleotides and polynucleotides thus produced are useful in bioconjugation reactions for the immobilization and purification of macromolecules.
摘要翻译：本发明提供可用于掺入修饰的寡核苷酸和多核苷酸的芳基硼酸试剂。由此产生的修饰的寡核苷酸和多核苷酸可用于大分子的固定和纯化的生物缀合反应。

5. 发明授权

US5876938A Use of boron-containing polynucleotides as diagnostic agents 失效
标题翻译：使用含硼多核苷酸作为诊断剂
公开(公告)号：US5876938A
公开(公告)日：1999-03-02
申请号：US837340
申请日：1997-04-11
申请人： Mark L. Stolowitz , Robert J. Kaiser
发明人： Mark L. Stolowitz , Robert J. Kaiser
IPC分类号： C12N15/09 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C12P19/34 , C12Q1/68 , C07H19/00 , C07H21/02
CPC分类号： C07H23/00 , C07H21/00 , C12P19/34
摘要： Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
摘要翻译：提供了可用于检测靶基因的杂交测定中的修饰的核苷酸和多核苷酸。修饰的多核苷酸含有至少一个硼酸部分，其在不影响双链体形成期间该碱基的氢键能力的位置处连接到核苷酸碱基。修饰的多核苷酸通常由天然存在的核苷酸和一个或多个修饰的核苷酸形成。

6. 发明授权

US5831046A Boronic acid-contaning nucleic acid monomers 失效
标题翻译：含硼酸的核酸单体
公开(公告)号：US5831046A
公开(公告)日：1998-11-03
申请号：US692429
申请日：1996-08-05
申请人： Mark L. Stolowitz , Robert J. Kaiser
发明人： Mark L. Stolowitz , Robert J. Kaiser
IPC分类号： C12N15/09 , C07H19/06 , C07H19/10 , C07H19/14 , C07H19/16 , C07H19/20 , C07H21/00 , C07H23/00 , C12P19/34 , C12Q1/68 , C07C51/10 , C07D213/00 , C07H19/00
CPC分类号： C07H23/00 , C07H21/00 , C12P19/34
摘要： Modified nucleotides and polynucleotides which are useful in hybridization assays for the detection of target genes are provided. The modified polynucleotides contain at least one boronic acid moiety which is attached to a nucleotide base in a position which does not interfere with the hydrogen bonding capabilities of that base during duplex formation. The modified polynucleotides are typically formed from naturally occurring nucleotides and one or more modified nucleotides.
摘要翻译：提供了可用于检测靶基因的杂交测定中的修饰的核苷酸和多核苷酸。修饰的多核苷酸含有至少一个硼酸部分，其在不影响双链体形成期间该碱基的氢键能力的位置处连接到核苷酸碱基。修饰的多核苷酸通常由天然存在的核苷酸和一个或多个修饰的核苷酸形成。

7. 发明授权

US5688928A Phenylboronic acid complexing reagents derived from aminosalicylic acid 失效
标题翻译：衍生自氨基水杨酸的苯硼酸络合试剂
公开(公告)号：US5688928A
公开(公告)日：1997-11-18
申请号：US480970
申请日：1995-06-07
申请人： Mark L. Stolowitz
发明人： Mark L. Stolowitz
IPC分类号： C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/44 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/02 , C07F5/04 , C07K5/02 , G01N33/543
CPC分类号： C07D207/452 , C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/54353
摘要： The present invention relates to a novel class of phenylboronic acid complexing reagents useful for the preparation of bioconjugates, and the method of making and using such reagents. In the present invention, in the place of prior art Avidin-Biotin and Digoxigenin-anti-Digoxigenin systems, phenylboronic acid complexing reagents are utilized in conjunction with phenylboronic acid reagents (many of which are known in the prior art) to facilitate chemical conjugation without the use of intermediary biological macromolecules. Reagents suitable for the modification of bioactive species for the purpose of incorporating a phenylboronic acid complexing moiety for subsequent conjugation to a different bioactive species having pendant phenylboronic acid moieties are of General Formula I or General Formula II. ##STR1## wherein group X is selected from either H, OH, NH.sub.2, NHCH.sub.3, NHOH and NHOCH.sub.3, wherein group Y is selected from either O, S, and NH, and wherein group Z comprises a spacer which either separates the boronic acid complexing moiety from group R, as in General Formula I, or seperates two boronic acid complexing moieties, as in General Formula II. Group R is a reactive electrophilic or nucleophilic moiety suitable for reaction of the phenylboronic acid complexing reagent with a bioactive species.
摘要翻译：本发明涉及可用于制备生物缀合物的新一类苯基硼酸络合剂，以及制备和使用这些试剂的方法。在本发明中，代替现有技术的抗生物素蛋白 - 生物素和地高辛 - 抗地高辛系统，苯硼酸络合试剂与苯硼酸试剂（许多已知在现有技术中）一起使用，以促进化学共轭而没有使用中间生物大分子。适于修饰生物活性物质的试剂为通式I或通式II，其用于将苯硼酸络合部分并入用于随后缀合至具有侧苯基硼酸部分的不同生物活性物质的目的。通式I其中基团X选自H，OH，NH 2，NHCH 3，NHOH和NHOCH 3，其中基团Y选自O，S和NH，并且其中基团Z包括如通式I所示，将硼酸络合部分与基团R分开的间隔物，或分离两个硼酸络合部分，如通式II所示。基团R是适用于苯基硼酸络合试剂与生物活性物质反应的反应性亲电或亲核部分。

8. 发明授权

US5677431A Phenylboronic acid complexes for bioconjugate preparation 失效
标题翻译：苯硼酸复合物用于生物缀合物的制备
公开(公告)号：US5677431A
公开(公告)日：1997-10-14
申请号：US482886
申请日：1995-06-07
申请人： Mark L. Stolowitz
发明人： Mark L. Stolowitz
IPC分类号： C07F5/02 , G01N33/543
CPC分类号： C07F5/025 , G01N33/54353
摘要： The present invention relates to a novel class of phenylboronic acid bioconjugate complexes derived from aminosalicylic acid, and the method of making and using such bioconjugate complexes. The bioconjugate complexes are in the form of the following general formulas; ##STR1## wherein group X is selected from the group consisting of O, NH, N-alkyl, NC.sub.6 H.sub.5, N-aryl, NCH.sub.2 -aryl, NCH.sub.2 CH.sub.2 OH, NCOCH.sub.2 CH.sub.2 OH, NOH, NO-alkyl and NOCH.sub.2 -aryl, wherein alkyl denotes a hydrocarbon moiety of from 1 to 4 carbons in length and which may be linear or branched, wherein aryl is selected from the group consisting of an aromatic ring, a substituted aromatic ring and a fused aromatic ring, wherein group Y is selected from the group consisting of O, S, NH, N-alkyl, N-aryl and NCH.sub.2 -aryl, wherein groups Z and Z* comprise a spacer selected from an alkyl and a polyethyleneglycol chain, of from 1 to 16 carbon equivalents in length, wherein the chain may contain intermediate amide and disulfide bonds, and and wherein groups BAS and BAS* are bioactive species.
摘要翻译：本发明涉及一类新型的衍生自氨基水杨酸的苯基硼酸生物缀合物复合物，以及制备和使用这种生物缀合物复合物的方法。生物共轭配合物为下列通式的形式：通用化学式I GE>通式III + TR 通式IV + TR 其中基团X选自O，NH，N-烷基，NC6H5，N-芳基，NCH 2 - 芳基，NCH 2 CH 2 OH，NCOCH 2 CH 2 OH，NOH，NO-烷基和NOCH 2 - 芳基，其中烷基表示长度为1至4个碳的烃部分，其可以是直链或支链的，其中芳基选自芳香环，取代芳环和稠合芳环，其中基团Y选自O，S，NH，N-烷基，N-芳基和NCH 2 - 芳基，其中基团Z和Z *包含选自烷基和聚乙二醇链的间隔长度为1至16碳当量的链，其中该链可含有中间体酰胺和二硫键，并且其中gro

9. 发明授权

US5594151A Phenylboronic acid complexing reagents derived from aminosalicylic acid 失效
标题翻译：衍生自氨基水杨酸的苯硼酸络合试剂
公开(公告)号：US5594151A
公开(公告)日：1997-01-14
申请号：US188531
申请日：1994-01-28
申请人： Mark L. Stolowitz
发明人： Mark L. Stolowitz
IPC分类号： C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/44 , C07D207/452 , C07D207/46 , C07D213/71 , C07F5/02 , C07F5/04 , C07K5/02 , G01N33/543
CPC分类号： C07D207/452 , C07C229/38 , C07C233/49 , C07C233/51 , C07C237/12 , C07C237/22 , C07C255/13 , C07C259/10 , C07C271/22 , C07C281/02 , C07D207/46 , C07D213/71 , C07F5/025 , C07K5/0207 , G01N33/54353
摘要： The present invention relates to a novel class of phenylboronic acid complexing reagents useful for the preparation of bioconjugates, and the method of making and using such reagents. In the present invention, in the place of prior art Avidin-Biotin and Digoxigenin-anti-Digoxigenin systems, phenylboronic acid complexing reagents are utilized in conjunction with phenylboronic acid reagents (many of which are known in the prior art) to facilitate chemical conjugation without the use of intermediary biological macromolecules. Reagents suitable for the modification of a bioactive species for the purpose of incorporating a phenylboronic acid complexing moiety for subsequent conjugation to a different bioactive species having pendant phenylboronic acid moleties are of General Formula I or General Formula II. ##STR1## wherein group X is selected from either H, OH, NH.sub.2, NHCH.sub.3, NHOH and NHOCH.sub.3, wherein group Y is selected from either O, S and NH, and wherein group Z comprises a spacer which either separates the boronic acid complexing moiety from group R, as in General Formula I, or seperates two boronic acid complexing moieties, as in General Formula II. Group R is a reactive electrophilic or nucleophilic moiety suitable for reaction of the phenylboronic acid complexing reagent with a bioactive species.
摘要翻译：本发明涉及可用于制备生物缀合物的新一类苯基硼酸络合剂，以及制备和使用这些试剂的方法。在本发明中，代替现有技术的抗生物素蛋白 - 生物素和地高辛 - 抗地高辛系统，苯硼酸络合试剂与苯硼酸试剂（许多已知在现有技术中）一起使用，以促进化学共轭而没有使用中间生物大分子。适于修饰生物活性物质的试剂为通式I或通式II，其用于将苯硼酸络合部分并入用于随后与不同具有侧苯基硼酸汽车侧生物活性物质的缀合物。通式I其中基团X选自H，OH，NH 2，NHCH 3，NHOH和NHOCH 3，其中基团Y选自O，S和NH，并且其中基团Z包含如通式I那样将硼酸络合部分与基团R分开的间隔物，或分离两个硼酸络合部分，如通式II所示。基团R是适用于苯基硼酸络合试剂与生物活性物质反应的反应性亲电或亲核部分。

10. 发明授权

US07208322B2 Sensor surfaces for detecting analytes 失效
标题翻译：用于检测分析物的传感器表面
公开(公告)号：US07208322B2
公开(公告)日：2007-04-24
申请号：US10116013
申请日：2002-04-02
申请人： Mark L. Stolowitz , Jean P. Wiley , Guisheng Li , Kevin Lund
发明人： Mark L. Stolowitz , Jean P. Wiley , Guisheng Li , Kevin Lund
IPC分类号： G01N33/553 , G01N27/00 , G01N33/567 , G01N33/566
CPC分类号： G01N33/54373 , B82Y15/00 , B82Y30/00 , G01N21/553 , G01N33/54353 , G01N33/553 , G01N2610/00 , Y10S436/806 , Y10S436/829 , Y10T436/143333
摘要： The present invention provides a sensor surface comprising: a substrate coated with a free electron metal; and a matrix layer disposed on the free electron metal, wherein the matrix layer comprises an organic compound having a boronic acid complexing moiety. The matrix is preferably a self-assembled monolayer (SAM), a mixed self-assembled monolayer (mSAM), or combinations thereof.
摘要翻译：本发明提供了一种传感器表面，包括：涂覆有自由电子金属的基底; 以及设置在所述自由电子金属上的基质层，其中所述基质层包含具有硼酸络合部分的有机化合物。基质优选为自组装单层（SAM），混合自组装单层（mSAM）或其组合。

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