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1. 发明授权

US09664619B2 Microfluidic device for storage and well-defined arrangement of droplets 有权
公开(公告)号：US09664619B2
公开(公告)日：2017-05-30
申请号：US12990102
申请日：2009-04-28
申请人： Christian Boehm , Amy Rowat , Sarah Koester , Jeremy Agresti , David A. Weitz
发明人： Christian Boehm , Amy Rowat , Sarah Koester , Jeremy Agresti , David A. Weitz
IPC分类号： B01L3/00 , G01N21/64 , B01L7/00 , G01N33/50
CPC分类号： G01N21/6452 , B01L3/502784 , B01L7/525 , B01L2200/0621 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1827 , B01L2400/0487 , B01L2400/086 , G01N33/5008
摘要： The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time-resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.

2. 发明申请

US20110190146A1 MICROFLUIDIC DEVICE FOR STORAGE AND WELL-DEFINED ARRANGEMENT OF DROPLETS 有权
标题翻译：用于储存和定义错误布置的微流体装置
公开(公告)号：US20110190146A1
公开(公告)日：2011-08-04
申请号：US12990102
申请日：2009-04-28
申请人： Christian Boehm , Amy Rowat , Sarah Koester , Jeremy Agresti , David A. Weitz
发明人： Christian Boehm , Amy Rowat , Sarah Koester , Jeremy Agresti , David A. Weitz
IPC分类号： C40B30/00 , C40B50/00 , C40B60/14
CPC分类号： G01N21/6452 , B01L3/502784 , B01L7/525 , B01L2200/0621 , B01L2300/0816 , B01L2300/0819 , B01L2300/087 , B01L2300/1827 , B01L2400/0487 , B01L2400/086 , G01N33/5008
摘要： The present invention relates to systems and methods for the arrangement of droplets in pre-determined locations. Many applications require the collection of time- resolved data. Examples include the screening of cells based on their growth characteristics or the observation of enzymatic reactions. The present invention provides a tool and related techniques which addresses this need, and which can be used in many other situations. The invention provides, in one aspect, a tool that allows for stable storage and indexing of individual droplets. The invention can interface not only with microfluidic/microscale equipment, but with macroscopic equipment to allow for the easy injection of liquids and extraction of sample droplets, etc.
摘要翻译：本发明涉及用于在预定位置中布置液滴的系统和方法。许多应用程序需要收集时间分辨数据。实例包括基于其生长特征或观察酶反应来筛选细胞。本发明提供了一种解决该需求的工具和相关技术，并且可以在许多其它情况下使用。本发明在一个方面提供一种允许单个液滴的稳定储存和分度的工具。本发明不仅可以与微流体/微型设备连接，而且可以与宏观设备相接合，以便容易地注入液体和提取样品液滴等。

3. 发明申请

US20100252118A1 MANIPULATION OF FLUIDS, FLUID COMPONENTS AND REACTIONS IN MICROFLUIDIC SYSTEMS 有权
标题翻译：微流控系统中流体，流体组分和反应的操作
公开(公告)号：US20100252118A1
公开(公告)日：2010-10-07
申请号：US12595107
申请日：2008-04-18
申请人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
发明人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
IPC分类号： F15C1/02
CPC分类号： B01L3/502784 , B01L3/502746 , B01L2200/0673 , B01L2300/0861 , B01L2300/087 , B01L2300/0877 , B01L2400/0487 , B01L2400/0688 , B01L2400/0694 , B01L2400/082 , F17D1/12 , G01N1/28 , G01N15/0272 , G01N15/1484 , G01N2015/0092 , Y10T137/0324 , Y10T137/0391 , Y10T137/0396 , Y10T137/2082 , Y10T137/218 , Y10T436/2575
摘要： Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.
摘要翻译：提供了用于操纵流体，流体组分和反应的微流体结构和方法。在一个方面，这种结构和方法可以允许产生精确体积的液滴，其可被存储/保持在装置的精确区域。在另一方面，本文所述的微流体结构和方法被设计用于在组件中容纳和定位组件，使得即使在操作之后也可以操纵组件并随后进行跟踪。例如，细胞可以以本文所述的微流体结构的排列约束，以便于在其生长期间和/或在它们繁殖之后进行跟踪。

4. 发明授权

US09029085B2 Assays and other reactions involving droplets 有权
标题翻译：测定和其他涉及液滴的反应
公开(公告)号：US09029085B2
公开(公告)日：2015-05-12
申请号：US12529926
申请日：2008-03-07
申请人： Jeremy Agresti , Liang-Yin Chu , David A. Weitz , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George Church
发明人： Jeremy Agresti , Liang-Yin Chu , David A. Weitz , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George Church
IPC分类号： C12Q1/68 , C12P19/34 , B01J13/00 , B01F3/08 , B01F13/00
CPC分类号： C12P19/34 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , B01L3/502784 , C12Q1/6834 , C12Q1/6848 , C12Q1/686 , G01N15/1404 , G01N2015/1006
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明通常涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可用于测定中，并且在某些实施方案中，液滴或乳液可被硬化以形成凝胶。在一些方面，可以使用凝胶进行异质测定。例如，液滴可以被硬化以形成凝胶，其中液滴包含细胞，DNA或其它合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以扩散通过凝胶，或者硬化的颗粒可以液化以形成液体状态，允许反应物与细胞相互作用。作为具体实例，包含在凝胶颗粒内的DNA可以进行PCR（聚合酶链式反应）扩增，例如通过使用能够在形成凝胶时结合凝胶的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

5. 发明授权

US08592221B2 Manipulation of fluids, fluid components and reactions in microfluidic systems 有权
公开(公告)号：US08592221B2
公开(公告)日：2013-11-26
申请号：US12595107
申请日：2008-04-18
申请人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
发明人： Seth Fraden , Hakim Boukellal , Yanwei Jia , Seila Selimovic , Amy Rowat , Jeremy Agresti , David A. Weitz
IPC分类号： G01F11/00
CPC分类号： B01L3/502784 , B01L3/502746 , B01L2200/0673 , B01L2300/0861 , B01L2300/087 , B01L2300/0877 , B01L2400/0487 , B01L2400/0688 , B01L2400/0694 , B01L2400/082 , F17D1/12 , G01N1/28 , G01N15/0272 , G01N15/1484 , G01N2015/0092 , Y10T137/0324 , Y10T137/0391 , Y10T137/0396 , Y10T137/2082 , Y10T137/218 , Y10T436/2575
摘要： Microfluidic structures and methods for manipulating fluids, fluid components, and reactions are provided. In one aspect, such structures and methods can allow production of droplets of a precise volume, which can be stored/maintained at precise regions of the device. In another aspect, microfluidic structures and methods described herein are designed for containing and positioning components in an arrangement such that the components can be manipulated and then tracked even after manipulation. For example, cells may be constrained in an arrangement in microfluidic structures described herein to facilitate tracking during their growth and/or after they multiply.

6. 发明申请

US20100136544A1 ASSAYS AND OTHER REACTIONS INVOLVING DROPLETS 有权
标题翻译：测量和其他涉及倾销的反应
公开(公告)号：US20100136544A1
公开(公告)日：2010-06-03
申请号：US12529926
申请日：2008-03-07
申请人： Jeremy Agresti , Liang-Yin Chu , David A. Weitz , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George Church
发明人： Jeremy Agresti , Liang-Yin Chu , David A. Weitz , Jin-Woong Kim , Amy Rowat , Morten Sommer , Gautam Dantas , George Church
IPC分类号： C12Q1/68 , C12Q1/04
CPC分类号： C12P19/34 , B01F3/0807 , B01F3/0811 , B01F13/0062 , B01F13/0071 , B01F2215/0037 , B01J13/0052 , B01J13/0065 , B01L3/502784 , C12Q1/6834 , C12Q1/6848 , C12Q1/686 , G01N15/1404 , G01N2015/1006
摘要： The present invention generally relates to droplets and/or emulsions, such as multiple emulsions. In some cases, the droplets and/or emulsions may be used in assays, and in certain embodiments, the droplet or emulsion may be hardened to form a gel. In some aspects, a heterogeneous assay can be performed using a gel. For example, a droplet may be hardened to form a gel, where the droplet contains a cell, DNA, or other suitable species. The gel may be exposed to a reactant, and the reactant may interact with the gel and/or with the cell, DNA, etc., in some fashion. For example, the reactant may diffuse through the gel, or the hardened particle may liquefy to form a liquid state, allowing the reactant to interact with the cell. As a specific example, DNA contained within a gel particle may be subjected to PCR (polymerase chain reaction) amplification, e.g., by using PCR primers able to bind to the gel as it forms. As the DNA is amplified using PCR, some of the DNA will be bound to the gel via the PCR primer. After the PCR reaction, unbound DNA may be removed from the gel, e.g., via diffusion or washing. Thus, a gel particle having bound DNA may be formed in one embodiment of the invention.
摘要翻译：本发明通常涉及液滴和/或乳液，例如多重乳液。在一些情况下，液滴和/或乳液可用于测定中，并且在某些实施方案中，液滴或乳液可被硬化以形成凝胶。在一些方面，可以使用凝胶进行异质测定。例如，液滴可以被硬化以形成凝胶，其中液滴包含细胞，DNA或其它合适的物质。凝胶可以暴露于反应物，并且反应物可以以某种方式与凝胶和/或与细胞，DNA等相互作用。例如，反应物可以扩散通过凝胶，或者硬化的颗粒可以液化以形成液体状态，允许反应物与细胞相互作用。作为具体实例，包含在凝胶颗粒内的DNA可以进行PCR（聚合酶链式反应）扩增，例如通过使用能够在形成凝胶时结合凝胶的PCR引物。当使用PCR扩增DNA时，一些DNA将通过PCR引物与凝胶结合。 PCR反应后，未结合的DNA可以从凝胶中去除，例如通过扩散或洗涤。因此，可以在本发明的一个实施方案中形成具有结合DNA的凝胶颗粒。

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