专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06423834B1 KDO aldolase and condensation reactions employed therewith 失效
标题翻译： KDO醛缩酶和与其一起使用的缩合反应
公开(公告)号：US06423834B1
公开(公告)日：2002-07-23
申请号：US09247164
申请日：1999-02-09
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H1500
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

2. 发明授权

US5869316A Biologically pure culture of aureobacterium barkeri KDO-37-2 失效
标题翻译：生物纯培养的巴氏杆菌KDO-37-2
公开(公告)号：US5869316A
公开(公告)日：1999-02-09
申请号：US767182
申请日：1996-12-16
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12N1/20
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkeri strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了分离自巴斯德氏菌杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

3. 发明授权

US5358859A KDO Aldolase and condensation reactions employed therewith 失效
标题翻译： KDO醛缩酶和与其一起使用的缩合反应
公开(公告)号：US5358859A
公开(公告)日：1994-10-25
申请号：US993140
申请日：1992-12-18
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The DKDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield. Additionally, protected forms of the KDO aldolase products, e.g. hexaacetyl 2-keto-3-deoxy-nonulosonic acid and pentaacetyl 2-keto-3-deoxy-octulosonic acid, may be decarboxylated to form the corresponding 2-deoxy-aldoses, e.g. 2-deoxy-octulose and 2-deoxy-heptulose respectively.
摘要翻译：公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）KDO醛缩酶（EC 4.1.2.23）。进一步公开了DKDO醛缩酶对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。另外，保护形式的KDO醛缩酶产物，例如，六乙酰基2-酮-3-脱氧 - 非酮酸和五乙酰基2-酮-3-脱氧 - 八环酸可以脱羧以形成相应的2-脱氧醛糖，例如， 2-脱氧 - 八酮糖和2-脱氧 - 七氟醚。

4. 发明授权

US5585261A KDO aldolase 失效
标题翻译： KDO醛缩酶
公开(公告)号：US5585261A
公开(公告)日：1996-12-17
申请号：US328739
申请日：1994-10-25
申请人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Takeshi Sugai , Gwo-Jenn Shen
IPC分类号： C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , C12P19/02 , C12N9/86 , C12N1/20
CPC分类号： C12P19/02 , C07H7/027 , C12N9/88 , C12P7/58 , C12P9/00 , Y10S435/822
摘要： Aureobacterium barkerei strain KDO-37-2 (ATCC 49977) and KDO aldolase (EC 4.1.2.23) isolated therefrom are disclosed. The KDO aldolase is further disclosed to have a broad substrate specificity with respect to its reverse reaction, i.e. the condensation of aldoses with pyruvate to form a wide range of 2-keto-3-deoxy-onic acids, including 2-keto-3-deoxy-nonulosonic acid, 2-keto-3-deoxy-octulosonic acid, 2-keto-3-deoxy-heptulosonic acid, and 2-keto-3-deoxy-hexulosonic acid. In particular, 3-deoxy-D-manno-2-octulosonic acid (D-KDO), a vital component of lipopolysaccharides found in the bacterial outer membrane may be synthesized from D-arabinose and pyruvate in 67% yield.
摘要翻译：公开了从其分离的巴氏杆菌菌株KDO-37-2（ATCC 49977）和KDO醛缩酶（EC 4.1.2.23）。进一步公开KDO醛缩酶相对于其逆反应具有广泛的底物特异性，即醛糖与丙酮酸的缩合形成宽范围的2-酮-3-脱氧ic酸，包括2-酮-3- 脱氧 - 非酮酸，2-酮-3-脱氧 - 八环酮酸，2-酮-3-脱氧 - 庚酮酸和2-酮-3-脱氧 - 己酸。特别地，在D-细菌外膜中发现的脂多糖的重要组分3-脱氧-D-壬烯-2-辛酮酸（D-KDO）可由D-阿拉伯糖和丙酮酸合成，产率67％。

5. 发明授权

US5278299A Method and composition for synthesizing sialylated glycosyl compounds 失效
标题翻译：合成唾液酸化糖基化合物的方法和组成
公开(公告)号：US5278299A
公开(公告)日：1994-01-11
申请号：US670701
申请日：1991-03-18
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
IPC分类号： C12N1/21 , C12N9/12 , C12P19/04 , C12P19/18 , C12P19/26 , C07H1/00 , C12N9/24
CPC分类号： C12N9/1241 , C12P19/04 , C12P19/18 , C12P19/26
摘要： The present invention provides a method for synthesizing a sialylated glycosyl compound comprising reacting in the presence of each other a sialic acid, a glycosyl compound, a CMP-sialic acid regenerating system, a pyrophosphate scavenger and catalytic amounts of a CMP-sialic acid synthetase and a sialyl transferase having substrate specificity for the glycosyl compound. The present invention also provides a composition for sialylating glycosyl compounds comprising a sialic acid, CMP-sialic acid regenerating system, a pyrophosphate scavenger and a catalytic amount of CMP-sialic acid synthetase. The composition can further comprise an aqueous solvent having a suitable buffer and enzyme cofactors as well as a catalytic amount of a sialyl transferase having substrate specificity for the glycosyl compound. A phagemid-transformed E. coli. that overproduces CMP-sialic acid synthetase is also disclosed.
摘要翻译：本发明提供了合成唾液酸化糖基化合物的方法，包括在唾液酸，糖基化合物，CMP-唾液酸再生系统，焦磷酸盐清除剂和催化量的CMP-唾液酸合成酶的存在下彼此反应，以及具有对糖基化合物具有底物特异性的唾液酸转移酶。本发明还提供了用于唾液酸化糖基化合物的组合物，其包含唾液酸，CMP-唾液酸再生系统，焦磷酸盐清除剂和催化量的CMP-唾液酸合成酶。组合物还可以包含具有合适的缓冲液和酶辅因子的水性溶剂以及催化量的对糖基化合物具有底物特异性的唾液酸转移酶。噬菌粒转化的大肠杆菌。也公开了过度生产CMP-唾液酸合成酶。

6. 发明授权

US06168934A Oligosaccharide enzyme substrates and inhibitors: methods and compositions 失效
标题翻译：寡糖酶底物和抑制剂：方法和组合物
公开(公告)号：US06168934A
公开(公告)日：2001-01-02
申请号：US09072958
申请日：1998-05-05
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
IPC分类号： C12P1918
CPC分类号： C12N9/1241 , C12P19/04 , C12P19/18 , C12P19/26
摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
摘要翻译：公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。还公开了糖基化的方法。用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

7. 发明授权

US07335500B2 Production of fucosylated carbohydrates by enzymatic fucosylation synthesis of sugar nucleotides; and in situ regeneration of GDP-fucose 失效
标题翻译：通过糖核苷酸的酶解岩藻糖基化合成生产岩藻糖基化碳水化合物; 和GDP-岩藻糖的原位再生
公开(公告)号：US07335500B2
公开(公告)日：2008-02-26
申请号：US09992680
申请日：2001-11-19
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen , Kun-Chin Liu
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen , Kun-Chin Liu
IPC分类号： C12P19/22
CPC分类号： C07H5/02 , C07H3/06 , C07H11/04 , C07H13/04 , C07H13/08 , C07H15/18 , C07H19/20 , C12P19/18 , C12P19/26
摘要： This invention contemplates improved methods of enzymatic production of carbohydrates especially fucosylated carbohydrates. Improved syntheses of glycosyl 1- or 2-phosphates using both chemical and enzymatic means are also contemplated. The phosphorylated glycosides are then used to produce sugar nucleotides that are in turn used as donor sugars for glycosylation of acceptor carbohydrates. Especially preferred herein is the use of a disclosed method for fucosylation.
摘要翻译：本发明考虑了碳水化合物，特别是岩藻糖基化碳水化合物的酶促生产的改进方法。还考虑了使用化学和酶学方法改进的1或2-磷酸糖基酯的合成。然后将磷酸化糖苷用于产生糖核苷酸，其又被用作供体糖用于受体碳水化合物的糖基化。本文特别优选使用所公开的岩藻糖基化方法。

8. 发明授权

US5759823A Ogligosaccharide enzyme substrates and inhibitors: methods and compostions 失效
标题翻译：寡糖酶底物和抑制剂：方法和组合物
公开(公告)号：US5759823A
公开(公告)日：1998-06-02
申请号：US472877
申请日：1995-06-07
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
IPC分类号： C07H3/06 , C07H7/027 , C07H13/06 , C12N1/21 , C12N9/12 , C12N15/09 , C12P19/04 , C12P19/18 , C12P19/26 , C12R1/19 , C12P19/12
CPC分类号： C12N9/1241 , C12P19/04 , C12P19/18 , C12P19/26
摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
摘要翻译：公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。还公开了糖基化的方法。用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

9. 发明授权

US5461143A Oligosaccharide enzyme substrates and inhibitors: methods and compositions 失效
标题翻译：寡糖酶底物和抑制剂：方法和组合物
公开(公告)号：US5461143A
公开(公告)日：1995-10-24
申请号：US219242
申请日：1994-03-29
申请人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Yoshitaka Ichikawa , Gwo-Jenn Shen
IPC分类号： C12N9/12 , C12P19/04 , C12P19/18 , C12P19/26 , C07H5/04 , C07H5/10
CPC分类号： C12N9/1241 , C12P19/04 , C12P19/18 , C12P19/26
摘要： Oligosaccharide compounds that are substrates and inhibitors of glycosyltransferase and glycosidase enzymes and compositions containing such compounds are disclosed. A method of glycosylation is also disclosed. An E. coli transformed with phagemid CMPSIL-1, which phagemid comprises a gene for a modified CMP-sialic acid synthetase enzyme, which transformed E. coli has the ATCC accession No. 68531 is also provided.
摘要翻译：公开了作为底物的寡糖化合物和糖基转移酶和糖苷酶的抑制剂以及含有这些化合物的组合物。还公开了糖基化的方法。用噬菌粒CMPSIL-1转化的大肠杆菌，该噬菌粒包含修饰的CMP-唾液酸合成酶的基因，转化的大肠杆菌也具有ATCC登录号68531。

10. 发明授权

US5919673A One-pot enzymatic sulfation process using 3'-phosphoadenosine-5'-phosphosulfate and recycled phosphorylated adenosine intermediates 失效
标题翻译：使用3'-磷酸腺苷-5'-磷酸硫酸盐和再循环的磷酸化腺苷中间体的一锅酶硫酸化方法
公开(公告)号：US5919673A
公开(公告)日：1999-07-06
申请号：US408774
申请日：1995-03-22
申请人： Chi-Huey Wong , Chun-Hung Lin , Gwo-Jenn Shen
发明人： Chi-Huey Wong , Chun-Hung Lin , Gwo-Jenn Shen
IPC分类号： C12N9/10 , C12N9/12 , C12P11/00 , C12P19/26 , C12P19/30 , C12P19/04 , C12P19/32 , C12P33/00
CPC分类号： C12N9/13 , C12N9/1205 , C12N9/1241 , C12P11/00 , C12P19/26 , C12P19/30
摘要： A process for the enzymatic synthase of sulfate esters is disclosed in which intermediate phosphorylated adenosine compounds are recycled to minimize enzyme inhibition. Exemplary enzymes include ATP sulfurylase, APS kinase and a sulfotransferase.
摘要翻译：公开了一种硫酸酯酶合成酶的方法，其中中间磷酸化腺苷化合物被循环以使酶抑制最小化。示例性的酶包括ATP硫酸化酶，APS激酶和磺基转移酶。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式