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    • 1. 发明授权
    • Two-dimensional microfluidics for protein separations and gene analysis
    • 用于蛋白质分离和基因分析的二维微流体
    • US07641780B2
    • 2010-01-05
    • US11086400
    • 2005-03-23
    • Cheng Sheng LeeDon DeVoe
    • Cheng Sheng LeeDon DeVoe
    • G01N27/453G01N30/02B01L3/00
    • C07K1/26C07K1/285
    • The invention provides a microfluidic apparatus for performing 2-D biomolecular separations. The microfluidic 2-D device may include first and second planar substrates which include at least a first dimension microchannel extending in a first direction and an array of second dimension microchannels extending in a second direction, preferably, orthogonal to the first dimension. The ends of at least some of the microchannels are in fluid communication with a plurality of reservoirs. The substrates may further include a number of microchannels and reservoirs. The reservoirs are in electrical communication with a plurality of electrodes and voltage power sources. The device enables two dimensional separations of proteins, DNA and other biomolecules. According to another aspect of the invention, an array of tertiary microchannels extending in a third direction may be utilized.
    • 本发明提供了用于进行2-D生物分子分离的微流体装置。 微流体2-D器件可以包括第一和第二平面衬底,其包括至少第一维度微通道,其沿第一方向延伸,并且第二维微通道阵列沿第二方向延伸,优选地与第一维度正交。 至少一些微通道的端部与多个储存器流体连通。 基板还可以包括多个微通道和储存器。 储存器与多个电极和电压电源电连通。 该装置能够对蛋白质,DNA和其他生物分子进行二维分离。 根据本发明的另一方面,可以利用沿第三方向延伸的三级微通道阵列。
    • 2. 发明授权
    • Plastic microfluidics enabling two-dimensional protein separations in proteome analysis
    • 塑料微流体使蛋白质组分析中的二维蛋白质分离
    • US06974526B2
    • 2005-12-13
    • US10135386
    • 2002-05-01
    • Cheng Sheng LeeDon DeVoe
    • Cheng Sheng LeeDon DeVoe
    • B81B1/00G01N27/447G01N27/453
    • G01N27/44795G01N27/44773G01N27/44791
    • The invention provides a microfluidic apparatus for performing 2-D biomolecular separations. The microfluidic 2-D device may include first and second planar substrates which include at least a first dimension microchannel extending in a first direction and an array of second dimension microchannels extending in a second direction, preferably, orthogonal to the first dimension. The ends of at least some of the microchannels are in fluid communication with a plurality of reservoirs. The substrates may further include a number of microchannels and reservoirs. The reservoirs are in electrical communication with a plurality of electrodes and voltage power sources. The device enables two dimensional separations of proteins and other biomolecules. According to another aspect of the invention, an isoelectric point based separation is enabled in a first dimension, and a size based separation in a second dimension.
    • 本发明提供了用于进行2-D生物分子分离的微流体装置。 微流体2-D器件可以包括第一和第二平面衬底,其包括至少第一维度微通道,其沿第一方向延伸,并且第二维微通道阵列沿第二方向延伸,优选地与第一维度正交。 至少一些微通道的端部与多个储存器流体连通。 基板还可以包括多个微通道和储存器。 储存器与多个电极和电压电源电连通。 该装置能够进行蛋白质和其他生物分子的二维分离。 根据本发明的另一方面,基于等电点的分离在第一维度和基于尺寸的第二维度的分离中得以实现。
    • 3. 发明申请
    • Two-dimensional microfluidics for protein separations and gene analysis
    • 用于蛋白质分离和基因分析的二维微流体
    • US20060054504A1
    • 2006-03-16
    • US11086400
    • 2005-03-23
    • Cheng LeeDon DeVoe
    • Cheng LeeDon DeVoe
    • C07K1/26G01N27/447
    • C07K1/26C07K1/285
    • The invention provides a microfluidic apparatus for performing 2-D biomolecular separations. The microfluidic 2-D device may include first and second planar substrates which include at least a first dimension microchannel extending in a first direction and an array of second dimension microchannels extending in a second direction, preferably, orthogonal to the first dimension. The ends of at least some of the microchannels are in fluid communication with a plurality of reservoirs. The substrates may further include a number of microchannels and reservoirs. The reservoirs are in electrical communication with a plurality of electrodes and voltage power sources. The device enables two dimensional separations of proteins, DNA and other biomolecules. According to another aspect of the invention, an array of tertiary microchannels extending in a third direction may be utilized.
    • 本发明提供了用于进行2-D生物分子分离的微流体装置。 微流体2-D器件可以包括第一和第二平面衬底,其包括至少第一维度微通道,其沿第一方向延伸,并且第二维微通道阵列沿第二方向延伸,优选地与第一维度正交。 至少一些微通道的端部与多个储存器流体连通。 基板还可以包括多个微通道和储存器。 储存器与多个电极和电压电源电连通。 该装置能够对蛋白质,DNA和其他生物分子进行二维分离。 根据本发明的另一方面,可以利用沿第三方向延伸的三级微通道阵列。