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1. 发明申请

US20130344550A1 METHODS OF BIOPLASTIC PRODUCTION 审中-公开
标题翻译：生物生产方法
公开(公告)号：US20130344550A1
公开(公告)日：2013-12-26
申请号：US13914461
申请日：2013-06-10
申请人： Charles Miller , Asif Rahman , Ronald Sims , Ashik Sathish , Renil Anthony
发明人： Charles Miller , Asif Rahman , Ronald Sims , Ashik Sathish , Renil Anthony
IPC分类号： C12P7/62
CPC分类号： C12P7/625 , C12N1/20 , C12N1/32
摘要： A method of producing bioplastics from algae, the method including processing algae to yield an aqueous phase containing glycerol, and fermenting the aqueous phase with a bioplastic-producing bacteria to yield bioplastics.
摘要翻译：一种从藻类生产生物塑料的方法，该方法包括处理藻类以产生含有甘油的水相，并用产生生物塑料的细菌发酵水相以产生生物塑料。

2. 发明申请

US20140011246A1 Methods for Harvesting and Processing Biomass 审中-公开
标题翻译：收获和加工生物质的方法
公开(公告)号：US20140011246A1
公开(公告)日：2014-01-09
申请号：US13915612
申请日：2013-06-11
申请人： Ronald Sims , Charles Miller , Joshua T. Ellis , Ashik Sathish , Renil Anthony , Asif Rahman
发明人： Ronald Sims , Charles Miller , Joshua T. Ellis , Ashik Sathish , Renil Anthony , Asif Rahman
IPC分类号： C12P7/64 , C12P5/02 , C12P7/28 , C12P7/16 , C12P7/62 , C12P7/06
CPC分类号： C12P7/649 , C12N1/02 , C12P5/023 , C12P7/065 , C12P7/16 , C12P7/28 , C12P7/625 , Y02E50/13 , Y02E50/17 , Y02E50/343
摘要： A system and method for harvesting and processing algae, the system and method including harvesting algae by mechanical or chemical system and processing the harvested algae to produce at least one of biodiesel, biosolvents, bioplastics, biogas, or fertilizer.
摘要翻译：用于收获和处理藻类的系统和方法，所述系统和方法包括通过机械或化学系统收获藻类并处理收获的藻类以产生生物柴油，生物溶剂，生物塑料，沼气或肥料中的至少一种。

3. 发明授权

US09169300B2 Methods, chimeric polypeptides, polynucleotides and cells for producing bioplastics 有权
标题翻译：方法，嵌合多肽，多核苷酸和用于生产生物塑料的细胞
公开(公告)号：US09169300B2
公开(公告)日：2015-10-27
申请号：US12907572
申请日：2010-10-19
申请人： Charles Miller , Ronald Sims , Elisabeth Linton
发明人： Charles Miller , Ronald Sims , Elisabeth Linton
IPC分类号： C12P7/62 , C07K19/00 , C07H21/00 , C12N15/63 , C07K14/195
CPC分类号： C07K14/195 , C07K2319/02 , C12P7/625
摘要： Methods of producing a bioplastic are disclosed. Furthermore, chimeric polypeptides useful to transport polyhydroxyalkanoates (PHAs) are disclosed, as are polynucleotides that code for the chimeric polypeptides, cells harboring the chimeric polypeptides and methods of transporting PHAs.
摘要翻译：公开生产生物塑料的方法。此外，公开了可用于转运聚羟基链烷酸酯（PHA）的嵌合多肽，以及编码嵌合多肽的多核苷酸，携带嵌合多肽的细胞和转运PHA的方法。

4. 发明申请

US20070134683A2 PROBES AND METHODS FOR IDENTIFYING POLYCYCLIC AROMATIC HYDROCARBON-DEGRADING MYCOBACTERIA 审中-公开
标题翻译：鉴定多环芳烃降解马铃薯的探索与方法
公开(公告)号：US20070134683A2
公开(公告)日：2007-06-14
申请号：US11446411
申请日：2006-06-02
申请人： Charles Miller , Anne Anderson , Ronald Sims
发明人： Charles Miller , Anne Anderson , Ronald Sims
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689
摘要： A method for determining whether a microorganism is capable of biodegrading a contaminant can include the following: providing a first set of DNA molecules consisting of fragments of genomic DNA of a contaminant-degrading mycobacterium; contacting under hybridizing conditions, the first set of DNA molecules with a second set of DNA molecules consisting of genomic DNA of a microorganism, wherein it is not known whether or not the microorganism biodegrades a contaminant; and detecting hybridization between the first set of DNA molecules and the second set of DNA molecules, wherein the hybridization between the first and second sets is an indication that the microorganism is capable of biodegrading a contaminant.
摘要翻译：用于确定微生物是否能够生物降解污染物的方法可以包括以下：提供由污染物降解分枝杆菌的基因组DNA片段组成的第一组DNA分子; 在杂交条件下接触第一组DNA分子与由微生物的基因组DNA组成的第二组DNA分子，其中不知道微生物是否生物降解污染物; 以及检测第一组DNA分子与第二组DNA分子之间的杂交，其中第一组和第二组之间的杂交是微生物能够生物降解污染物的指示。

5. 发明申请

US20060275887A1 Mycobacteria compositions and methods of use in bioremediation 审中-公开
标题翻译：分枝杆菌组合物和生物修复中使用的方法
公开(公告)号：US20060275887A1
公开(公告)日：2006-12-07
申请号：US11446274
申请日：2006-06-02
申请人： Charles Miller , Anne Anderson , Ronald Sims
发明人： Charles Miller , Anne Anderson , Ronald Sims
IPC分类号： C12N1/21
CPC分类号： C12N1/20 , B09C1/10 , B09C1/105 , C12N9/0071
摘要： The present invention includes a contaminant-degrading composition for use in remediation of contaminated soil having a selected contaminant. Such a composition can include a seed for a plant capable of growing in the presence of the selected contaminant, and a contaminant-degrading mycobacteria on the seed. Additionally, the present invention includes a contaminant-degrading system for use in remediation of contaminated soil having a selected contaminant. Such a system can include a plant growing in the contaminated soil, and contaminant-degrading mycobacteria colonized on a root of the plant, wherein the mycobacteria is capable of degrading the selected contaminant. The mycobacteria can be capable of degrading the selected contaminant, such as PAHs, PCPs, MTBEs, and the like. Additionally, the contaminant-degrading mycobacteria can be at least one of M. KMS, M. JLS, or M MCS. Also, the contaminant-degrading mycobacteria can have nid dioxygenase genes, which can further include a nidB-nidA sequence motif.
摘要翻译：本发明包括用于修复具有选定污染物的污染土壤的污染物降解组合物。这样的组合物可以包括能够在所选择的污染物存在下生长的植物的种子和种子上的污染物降解分枝杆菌。另外，本发明包括污染物降解系统，其用于修复具有选定污染物的污染土壤。这样的系统可以包括在受污染的土壤中生长的植物和定植在植物根部的污染降解分枝杆菌，其中分枝杆菌能够降解所选择的污染物。分枝杆菌能够降解所选择的污染物，例如PAH，PCP，MTBE等。此外，污染降解分枝杆菌可以是M.KMS，M.JLS或M MCS中的至少一种。此外，降解污染物的分枝杆菌可以具有nid双加氧酶基因，其可以进一步包括nidB-nidA序列基序。

6. 发明申请

US20060275814A1 Probes and methods for identifying polycyclic aromatic hydrocarbon-degrading mycobacteria 审中-公开
标题翻译：用于鉴定多环芳烃降解分枝杆菌的探针和方法
公开(公告)号：US20060275814A1
公开(公告)日：2006-12-07
申请号：US11446411
申请日：2006-06-02
申请人： Charles Miller , Anne Anderson , Ronald Sims
发明人： Charles Miller , Anne Anderson , Ronald Sims
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/689
摘要： A method for determining whether a microorganism is capable of biodegrading a contaminant can include the following: providing a first set of DNA molecules consisting of fragments of genomic DNA of a contaminant-degrading mycobacterium; contacting under hybridizing conditions, the first set of DNA molecules with a second set of DNA molecules consisting of genomic DNA of a microorganism, wherein it is not known whether or not the microorganism biodegrades a contaminant; and detecting hybridization between the first set of DNA molecules and the second set of DNA molecules, wherein the hybridization between the first and second sets is an indication that the microorganism is capable of biodegrading a contaminant.
摘要翻译：用于确定微生物是否能够生物降解污染物的方法可以包括以下：提供由污染物降解分枝杆菌的基因组DNA片段组成的第一组DNA分子; 在杂交条件下接触第一组DNA分子与由微生物的基因组DNA组成的第二组DNA分子，其中不知道微生物是否生物降解污染物; 以及检测第一组DNA分子与第二组DNA分子之间的杂交，其中第一组和第二组之间的杂交是微生物能够生物降解污染物的指示。

7. 发明申请

US20130337548A1 Rotating Bioreactor 审中-公开
标题翻译：旋转生物反应器
公开(公告)号：US20130337548A1
公开(公告)日：2013-12-19
申请号：US13952469
申请日：2013-07-26
申请人： Ronald Sims , Reese Thompson , Steven T. Goldsmith , Brad Hansen , Terence Smith , Logan Christenson
发明人： Ronald Sims , Reese Thompson , Steven T. Goldsmith , Brad Hansen , Terence Smith , Logan Christenson
IPC分类号： C12N1/12
CPC分类号： C12N1/12 , C12M21/02 , C12M25/02 , C12M27/10 , C12M33/20
摘要： A bioreactor, including at least one rotating drum, a substrate on the drum whereon a biofilm may grow, a rotational device rotating the drum and substrate, and a harvesting device.
摘要翻译：生物反应器，包括至少一个旋转鼓，生物膜可能生长的滚筒上的基底，旋转滚筒和基底的旋转装置和收获装置。

8. 发明申请

US20110217764A1 Rotating Bioreactor and Spool Harvester Apparatus for Biomass Production 审中-公开
标题翻译：用于生物质生产的旋转生物反应器和卷轴收获机
公开(公告)号：US20110217764A1
公开(公告)日：2011-09-08
申请号：US13040364
申请日：2011-03-04
申请人： Logan Christenson , Ronald Sims
发明人： Logan Christenson , Ronald Sims
IPC分类号： C12M1/10
CPC分类号： C12M1/10
摘要： An apparatus that exposes a biofilm growth surface to liquid media as it rotates. A biofilm growth substratum is wound around a rotatable body in the form of a non-rigid material capable of supporting biofilm growth. A harvester receives the biofilm laden substratum, collects the biofilm as a biomass and reloads the substratum onto the rotatable body.
摘要翻译：一种将生物膜生长表面暴露于液体介质旋转的装置。生物膜生长基底以能够支持生物膜生长的非刚性材料的形式缠绕在可旋转体上。收割机接收载有生物膜的底层，收集生物膜作为生物质，并将底物重新装载到可旋转体上。

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