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1. 发明申请

US20130122606A1 METHOD AND DEVICE FOR CHEMICAL AND/OR BIOLOGICAL ANALYSIS 审中-公开
标题翻译：化学和/或生物分析的方法和装置
公开(公告)号：US20130122606A1
公开(公告)日：2013-05-16
申请号：US13810998
申请日：2011-07-28
申请人： Elisabeth Cerrato , Sylvie Cheucle , Bruno Colin , Virginie Helleux , Cecile Paris
发明人： Elisabeth Cerrato , Sylvie Cheucle , Bruno Colin , Virginie Helleux , Cecile Paris
IPC分类号： G01N33/53
CPC分类号： G01N33/5306 , B01F11/0074 , B01L3/0293 , B01L2400/0433 , B01L2400/0487 , G01N2035/1055 , G01N2035/106
摘要： A method of chemical and/or biological analysis including: providing a container delimited by a wall including an inner surface on which there is deposited an active component intended to make possible a chemical and/or biological reaction with a solution disposed in the container; filling the container with a liquid solution containing an analyte which is to be detected; and in applying to the liquid a succession of movements of aspirating/discharging a volume in the container at a suitable frequency to bring about resonance of the liquid so as to deform the free surface thereof and to result in a preferential distribution of the fluid along the inner surface of the wall of the container.
摘要翻译：一种化学和/或生物分析方法，包括：提供由壁限定的容器，所述壁包括内表面，在该内表面上沉积有效成分，以使得与设置在容器中的溶液形成化学和/或生物反应; 用含有待检测分析物的液体溶液填充容器; 并且在液体中以适当的频率向液体中施加吸入/排出容器的一系列运动，以使液体的共振发生变化，从而使流体沿其自由表面发生优先分配容器壁的内表面。

2. 发明申请

US20080152541A1 Integrated Analysis Device Which Can Be Fitted To A Container Housing A Sample To Be Analyzed 有权
标题翻译：综合分析装置可以适用于集装箱住房要分析的样本
公开(公告)号：US20080152541A1
公开(公告)日：2008-06-26
申请号：US11597867
申请日：2005-06-24
申请人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
发明人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
IPC分类号： G01N30/00 , B01J19/00 , G01N35/10
CPC分类号： B01L3/505 , B01L2300/044 , B01L2300/0663 , B01L2300/0672 , B01L2300/0825 , B01L2400/0406 , Y10T436/110833 , Y10T436/112499
摘要： The present invention relates generally to the field of analysis, for example biological analysis. More specifically, the present invention relates to an analysis device (10, 40, 501, 60) designed to be associated with a container (22, 50, 70) containing a sample (28) to be analyzed, said device comprising essentially: a first impervious membrane (12, 42, 52, 62); a second impervious membrane (14, 44, 54, 64) superposed on the first membrane (12, 42, 52, 62); said first and second membranes being fixedly attached over at least a part of their surface, defining a peripheral zone, in order to define an interstitial space forming an internal reaction cavity (16, 46, 56, 66), with no means of fluid communication with the outside of said analysis device (10, 40, 501, 60); at least one analysis means (18, 47, 57, 67), placed inside said internal reaction cavity (16, 46, 56, 66), designed to be placed in contact with the sample (28); at least one means designed to create a way for the sample to enter said internal reaction cavity. The present invention also relates to a container (22, 50, 70) comprising such an analysis device.The present invention also relates to a method of analyzing a sample contained in a container, in which an analysis device according to the invention is used.
摘要翻译：本发明一般涉及分析领域，例如生物分析。更具体地说，本发明涉及一种设计成与容纳待分析样品（28）的容器（22,50,70）相关联的分析装置（10,40,501,60），所述装置基本上包括：第一不透水膜（12,42,52,62）; 叠置在第一膜（12,42,52,62）上的第二不透水膜（14,44,54,64）; 所述第一和第二膜固定地附接在其表面的至少一部分上，限定了周边区域，以便限定形成内部反应腔（16,46,56,66）的间隙空间，没有任何流体连通与所述分析装置的外部（10,40,501,60）; 放置在与所述样品（28）接触的所述内部反应腔（16,46,56,66）内的至少一个分析装置（18,47,57,67）; 至少一种设计用于创建样品进入所述内部反应腔的方式的装置。本发明还涉及包括这种分析装置的容器（22,50,70）。本发明还涉及一种分析包含在容器中的样品的方法，其中使用了根据本发明的分析装置。

3. 发明授权

US09562260B2 Nucleic acid amplification reaction station for disposable test devices 有权
标题翻译：一次性试验装置的核酸扩增反应站
公开(公告)号：US09562260B2
公开(公告)日：2017-02-07
申请号：US12924351
申请日：2010-09-24
申请人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catazariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacque Dachaud
发明人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catazariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacque Dachaud
IPC分类号： B01L3/00 , A01B1/00 , C12Q1/68 , B01L7/00 , G01N35/02 , G01N35/04
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
摘要翻译：用于在一次性测试装置中进行核酸扩增反应的仪器。测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。仪器包括接收测试装置的支撑结构。温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

4. 发明授权

US08518342B2 Integrated analysis device which can be fitted to a container housing a sample to be analyzed 有权
标题翻译：集成分析装置，其可以装配到容纳待分析样品的容器中
公开(公告)号：US08518342B2
公开(公告)日：2013-08-27
申请号：US11597867
申请日：2005-06-24
申请人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
发明人： David Mosticone , Pascal Montes , Jean-Claude Raymond , Bruno Colin , Cecile Paris
IPC分类号： G01N21/75
CPC分类号： B01L3/505 , B01L2300/044 , B01L2300/0663 , B01L2300/0672 , B01L2300/0825 , B01L2400/0406 , Y10T436/110833 , Y10T436/112499
摘要： The present invention relates generally to the field of analysis, for example biological analysis. More specifically, the present invention relates to an analysis device (10, 40, 501, 60) designed to be associated with a container (22, 50, 70) containing a sample (28) to be analyzed, said device comprising essentially: a first impervious membrane (12, 42, 52, 62); a second impervious membrane (14, 44, 54, 64) superposed on the first membrane (12, 42, 52, 62); said first and second membranes being fixedly attached over at least a part of their surface, defining a peripheral zone, in order to define an interstitial space forming an internal reaction cavity (16, 46, 56, 66), with no means of fluid communication with the outside of said analysis device (10, 40, 501, 60); at least one analysis means (18, 47, 57, 67), placed inside said internal reaction cavity (16, 46, 56, 66), designed to be placed in contact with the sample (28); at least one means designed to create a way for the sample to enter said internal reaction cavity. The present invention also relates to a container (22, 50, 70) comprising such an analysis device.The present invention also relates to a method of analyzing a sample contained in a container, in which an analysis device according to the invention is used.
摘要翻译：本发明一般涉及分析领域，例如生物分析。更具体地说，本发明涉及一种设计成与容纳待分析样品（28）的容器（22,50,70）相关联的分析装置（10,40,501,60），所述装置基本上包括：第一不透水膜（12,42,52,62）; 叠置在第一膜（12,42,52,62）上的第二不透水膜（14,44,54,64）; 所述第一和第二膜固定地附接在其表面的至少一部分上，限定了周边区域，以便限定形成内部反应腔（16,46,56,66）的间隙空间，没有任何流体连通与所述分析装置的外部（10,40,501,60）; 放置在与所述样品（28）接触的所述内部反应腔（16,46,56,66）内的至少一个分析装置（18,47,57,67）; 至少一种设计用于创建样品进入所述内部反应腔的方式的装置。本发明还涉及包括这种分析装置的容器（22,50,70）。本发明还涉及一种分析包含在容器中的样品的方法，其中使用了根据本发明的分析装置。

5. 发明授权

US07807449B2 Nucleic acid amplification reaction station for disposable test devices 有权
标题翻译：一次性试验装置的核酸扩增反应站
公开(公告)号：US07807449B2
公开(公告)日：2010-10-05
申请号：US11438938
申请日：2006-05-22
申请人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catazariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacque Dachaud
发明人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catazariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacque Dachaud
IPC分类号： C12M1/34 , C12M3/00 , C12M1/36 , C12M1/38
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
摘要翻译：用于在一次性测试装置中进行核酸扩增反应的仪器。测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。仪器包括接收测试装置的支撑结构。温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

6. 发明申请

US20130224884A1 DEVICE AND METHOD FOR IMMUNOASSAYS 审中-公开
标题翻译：用于免疫功能的装置和方法
公开(公告)号：US20130224884A1
公开(公告)日：2013-08-29
申请号：US13881960
申请日：2011-11-16
申请人： Helene Briand , Bruno Colin , Cecile Paris
发明人： Helene Briand , Bruno Colin , Cecile Paris
IPC分类号： G01N33/558
CPC分类号： G01N33/558 , G01N33/54386 , G01N33/56988 , G01N33/5764 , G01N33/6854 , G01N2333/02 , G01N2333/11 , G01N2333/16
摘要： A device for performing a test to determine the presence or the absence of at least one analyte in a liquid sample includes: a support and a matrix, fixed on the support. The matrix includes: a liquid sample application zone, a marking zone and at least one reaction zone. The one reaction zone includes: a test results display zone having at least a second immobilized binding partner which is able to bind to at least one analyte, and a monitoring zone downstream of the results display zone or parallel with the results display zone which allows monitoring of the correct functioning of the device and which includes at least one analogue of the at least one analyte which is able to bind to at least a first marked binding partner. The liquid sample application zone, marking zone and reaction zone are in fluid communication.
摘要翻译：用于进行测试以确定液体样品中至少一种分析物的存在或不存在的装置包括：固定在载体上的载体和基质。该基质包括：液体样品施加区域，标记区域和至少一个反应区域。一个反应区包括：测试结果显示区，其具有能够结合至少一种分析物的至少第二固定化结合配偶体，以及结果显示区域下游的监测区域，或者与结果显示区域平行，允许监测的装置的正确功能，并且其包括能够结合至少第一标记结合配偶体的至少一种分析物的至少一种类似物。液体样品应用区，标记区和反应区处于流体连通状态。

7. 发明申请

US20060078466A1 Device for implementing an analysis pack, analysis pack and method using same 失效
标题翻译：用于实现分析包，分析包和使用其的方法的设备
公开(公告)号：US20060078466A1
公开(公告)日：2006-04-13
申请号：US11251758
申请日：2005-10-18
申请人： Bruno Colin , Jacques Dachaud , Marie Privat , Cecile Paris
发明人： Bruno Colin , Jacques Dachaud , Marie Privat , Cecile Paris
IPC分类号： G01N15/06
CPC分类号： G01N35/1097 , B01L3/5025
摘要： The invention concerns a device for implementing a test card inside which sequential reactions and fluid transfer operations are performed under the effect of a control means integrated into the card; said card includes at least two sequential reaction systems arranged in parallel, each reaction system consisting of at least two fluid transfer systems arranged in series. The invention also concerns a test card and the method for implementing the device and the card. The device is characterized in that it contains at least one actuator per reaction line, and all the actuators are at a regular distance from one another, with the actuators preferably being equidistant. The invention is particularly applicable for the micromanipulation of fluids in biological applications.
摘要翻译：本发明涉及一种用于实现测试卡的设备，其中在集成到卡中的控制装置的作用下执行顺序反应和流体传递操作; 所述卡包括平行布置的至少两个连续的反应系统，每个反应系统由串联布置的至少两个流体传输系统组成。本发明还涉及测试卡和用于实现设备和卡的方法。该装置的特征在于，其每个反应管线包含至少一个致动器，并且所有致动器彼此间隔一定距离，致动器优选为等距离的。本发明特别适用于生物应用中流体的显微操作。

8. 发明申请

US20130273592A1 METHOD FOR ISOLATING A SAMPLE WELL OF A TEST CARD FOR ANALYSIS, AND RESULTING TEST CARD 有权
标题翻译：用于分析测试卡的样品的分析方法和分析测试卡
公开(公告)号：US20130273592A1
公开(公告)日：2013-10-17
申请号：US13879887
申请日：2011-10-19
申请人： Bruno Colin , Cecile Paris
发明人： Bruno Colin , Cecile Paris
IPC分类号： C12Q1/24
CPC分类号： B01L3/50853 , B01L3/502738 , B01L2200/0689 , B01L2300/0864 , B01L2300/0887 , B01L2400/0481 , B01L2400/0638 , B01L2400/0655 , C12Q1/24
摘要： The invention relates to a method for isolating at least one fluid sample well laid out in a test card for analyses, said test card including a plate (3) having at least one first main face from which is laid out at least one channel communicating with at least said well, this first face being coated with a membrane (17) provided with an adhesive (18) and which will cover said channel with a covering area (171), the method for isolation including a phase for introducing a fluid sample into at least said well and a phase for isolating said well consisting of exerting a force on at least one portion of the covering area (171) of the adhesive membrane (17) for ensuring displacement of the adhesive into an obturation area for the sample well.
摘要翻译：本发明涉及一种用于分离在测试卡中布置的至少一个流体样本的方法，用于分析，所述测试卡包括具有至少一个第一主面的板（3），所述板（3）布置在与至少所述井，该第一面涂覆有具有粘合剂（18）的膜（17），并且其将覆盖所述通道并具有覆盖区域（171），所述隔离方法包括用于将流体样品引入的相至少所述井以及用于隔离所述井的相，其包括在所述粘合膜（17）的覆盖区域（171）的至少一部分上施加力，以确保所述粘合剂移动到所述样品井的封闭区域。

9. 发明申请

US20110020921A1 Nucleic acid amplification reaction station for disposable test devices 有权
标题翻译：一次性试验装置的核酸扩增反应站
公开(公告)号：US20110020921A1
公开(公告)日：2011-01-27
申请号：US12924351
申请日：2010-09-24
申请人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachaud
发明人： Bryan W. Kluttz , Geoff A. McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachaud
IPC分类号： C12M1/34
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
摘要翻译：用于在一次性测试装置中进行核酸扩增反应的仪器。测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。仪器包括接收测试装置的支撑结构。温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

10. 发明申请

US20060263871A1 Nucleic acid applification reaction station for disposable test devices 有权
标题翻译：一次性试验装置的核酸应用反应站
公开(公告)号：US20060263871A1
公开(公告)日：2006-11-23
申请号：US11438938
申请日：2006-05-22
申请人： Bryan Kluttz , Geoff McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachuad
发明人： Bryan Kluttz , Geoff McKinley , Fabio Gennari , Michel Guy , Christopher Cotter , Luigi Catanzariti , Louis Graziano , Bruno Colin , Cecile Paris , Jacques Dachuad
IPC分类号： C12M1/36 , C12M1/34
CPC分类号： C12Q1/6848 , B01L3/502 , B01L7/52 , B01L7/525 , B01L2200/026 , B01L2200/16 , B01L2300/044 , B01L2300/0809 , B01L2300/0825 , B01L2300/0861 , B01L2300/087 , B01L2300/1822 , B01L2400/0487 , B01L2400/049 , B01L2400/0633 , B01L2400/0644 , B01L2400/0655 , G01N35/026 , G01N2035/0436
摘要： An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature. A pneumatic system is also provided that assists in fluid transfer of a reaction solution from the first chamber to the second chamber.
摘要翻译：用于在一次性测试装置中进行核酸扩增反应的仪器。测试装置包括含有第一核酸扩增试剂（例如引物和核苷酸）的第一反应室和含有或与第二核酸扩增试剂（例如，扩增酶如 RT）。仪器包括接收测试装置的支撑结构。温度控制系统将第一反应室保持在第一升高温度，同时将第二核酸扩增试剂保持在低于第一温度的第二温度，以便保留第二核酸扩增试剂。致动器在测试装置中的流体导管上操作，以在第一温度下在第一反应室中发生反应之后使第一和第二反应室彼此流体连通。还提供了一种有助于将反应溶液从第一室流体转移到第二室的气动系统。

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