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    • 1. 发明申请
    • CYTOPLASM TO ORGANELLE DELIVERY SYSTEM AND ASSOCIATED METHODS
    • CYTOPLASM到ORGANELLE交付系统和相关方法
    • US20130177988A1
    • 2013-07-11
    • US13681260
    • 2012-11-19
    • Brigham Young University
    • Quentin T. AtenRegis A. DavidSandra H. BurnettBrian D. JensenLarry L. Howell
    • C12N13/00
    • C12N15/8775C12N13/00C12N15/89
    • Systems, devices, and methods for delivering a biological material into an organelle of a cell are provided. In one aspect, for example, a method for introducing biological material into an organelle of a cell can include bringing into proximity outside of a cell a lance and a preselected biological material, charging the lance with a polarity and a charge sufficient to electrically associate the preselected biological material with a tip portion of the lance, and penetrating an outer portion of the cell with the lance and directing and inserting the lance into the cell but outside of the organelle. The method can further include discharging the lance to release at least a portion of the biological material, charging the lance with an opposite polarity and charge sufficient to electrophoretically drive at least a portion of the biological material into the organelle, and withdrawing the lance from the cell.
    • 提供了将生物材料递送到细胞的细胞器中的系统,装置和方法。 在一个方面,例如,将生物材料引入细胞的细胞器的方法可以包括使细胞附着在喷枪和预选的生物材料的外部,用足够的极性和电荷对喷枪进行充电以电连接 预先选择的具有喷枪的尖端部分的生物材料,并且用喷枪穿透细胞的外部部分并且将喷枪引导并插入细胞中,但是在细胞器外部。 该方法还可以包括排出喷枪以释放生物材料的至少一部分,以相反的极性对喷枪进行充电,并充入足以将至少一部分生物材料电泳驱动到细胞器中,并将枪从 细胞。
    • 2. 发明申请
    • CYTOPLASMA TO ORGANELLE DELIVERY SYSTEM AND ASSOCIATED METHODS
    • CYTOPLASMA到ORGANELLE交付系统和相关方法
    • US20140127813A1
    • 2014-05-08
    • US14058025
    • 2013-10-18
    • Brigham Young University
    • Quentin T. AtenRegis A. DavidSandra H. BurnettBrian D. JensenLarry L. Howell
    • C12N15/877
    • C12N15/8775C12N13/00C12N15/89
    • Systems, devices, and methods for delivering a biological material into an organelle of a cell are provided. In one aspect, for example, a method for introducing biological material into an organelle of a cell can include bringing into proximity outside of a cell a lance and a preselected biological material, charging the lance with a polarity and a charge sufficient to electrically associate the preselected biological material with a tip portion of the lance, and penetrating an outer portion of the cell with the lance and directing and inserting the lance into the cell but outside of the organelle. The method can further include discharging the lance to release at least a portion of the biological material, charging the lance with an opposite polarity and charge sufficient to electrophoretically drive at least a portion of the biological material into the organelle, and withdrawing the lance from the cell.
    • 提供了将生物材料递送到细胞的细胞器中的系统,装置和方法。 在一个方面,例如,将生物材料引入细胞的细胞器的方法可以包括使细胞附着在喷枪和预选的生物材料的外部,用足够的极性和电荷对喷枪进行充电以电连接 预先选择的具有喷枪的尖端部分的生物材料,并且用喷枪穿透细胞的外部部分并且将喷枪引导并插入细胞中,但是在细胞器外部。 该方法还可以包括排出喷枪以释放生物材料的至少一部分,以相反的极性对喷枪进行充电,并充入足以将至少一部分生物材料电泳驱动到细胞器中,并将枪从 细胞。