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1. 发明授权

US07238476B2 Methods and compositions for genotyping 失效
标题翻译：用于基因分型的方法和组合物
公开(公告)号：US07238476B2
公开(公告)日：2007-07-03
申请号：US10632393
申请日：2003-08-01
申请人： Brian McKeown , Roger Derbyshire , Paul Rowan , Robert Sung
发明人： Brian McKeown , Roger Derbyshire , Paul Rowan , Robert Sung
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6858 , C12Q2545/101 , C12Q2525/155
摘要： The invention provides compositions and methods for performing primer extension reactions, including employment of amplification primers having 5′ tags to incorporate into amplicons variant nucleotides of interest from target nucleic acids at known ratios, with or without sequences surrounding the variant nucleotides of interest. The invention provides identifying the variant nucleotides generated from the target nucleic acid and generated from the 5′ tags, comparing the results, evaluating the efficiency of the primer extension reactions, and monitoring the efficacy of such reactions. The invention accounts for DNA sequence and experimental variables that may affect efficiency of incorporation of nucleotides, and provides a reference point for the interpretation of polymorphisms. The invention also provides methods of breeding scrapie-resistant sheep populations.
摘要翻译：本发明提供了用于进行引物延伸反应的组合物和方法，包括使用具有5'标签的扩增引物，以具有或不具有围绕所关注的变体核苷酸的序列，以目标核酸以已知比例掺入感兴趣的扩增子变体核苷酸。本发明提供鉴定从靶核酸产生并从5'标签产生的变体核苷酸，比较结果，评估引物延伸反应的效率和监测这些反应的功效。本发明涉及可能影响核苷酸掺入效率的DNA序列和实验变量，并为多态性的解释提供参考。本发明还提供了育种抗瘟病羊种群的方法。

2. 发明授权

US07192700B2 Methods and compositions for conducting primer extension and polymorphism detection reactions 失效
标题翻译：用于进行引物延伸和多态性检测反应的方法和组合物
公开(公告)号：US07192700B2
公开(公告)日：2007-03-20
申请号：US10328150
申请日：2002-12-20
申请人： Brian McKeown
发明人： Brian McKeown
IPC分类号： C12Q1/68 , C12P19/34 , C07H21/04
CPC分类号： C12Q1/6858 , C12Q2525/185
摘要： The invention comprises compositions and methods for performing primer extension reactions, including employment of amplification primers having 5′ tags to incorporate into amplicons variant nucleotides of interest from target nucleic acids at known ratios, along with the sequences surrounding the variant nucleotides of interest. The invention comprises identifying the variant nucleotides generated from the target nucleic acid and generated from the 5′ tags, comparing the results, evaluating the efficiency of the primer extension reactions, and monitoring the efficacy of such reactions. The invention accounts for DNA sequence and experimental variables which may affect efficiency of incorporation of nucleotides, and provides a reference point for the interpretation of polymorphisms.
摘要翻译：本发明包括用于进行引物延伸反应的组合物和方法，包括使用具有5'标签的扩增引物以及已知比例的目的核酸的目标扩增子变体核苷酸以及围绕所关注的变体核苷酸的序列。本发明包括鉴定从靶核酸产生并从5'标签产生的变体核苷酸，比较结果，评估引物延伸反应的效率和监测这些反应的功效。本发明涉及可能影响核苷酸掺入效率的DNA序列和实验变量，为解释多态性提供参考。

3. 发明申请

US20060094010A1 Methods and compositions for analyzing comprised sample using single nucleotide polymorphism panels 审中-公开
标题翻译：使用单核苷酸多态性面板分析包含样品的方法和组合物
公开(公告)号：US20060094010A1
公开(公告)日：2006-05-04
申请号：US10519224
申请日：2003-06-26
申请人： Robert Giles , Jeanine Baisch , Brian McKeown
发明人： Robert Giles , Jeanine Baisch , Brian McKeown
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q1/6876 , C12Q1/6881 , C12Q2600/156 , C12Q2600/16
摘要： The present invention provides methods and compositions for analyzing compromised nucleic acid samples. The present invention also includes methods of selecting panels and panels of single nucleotide polymorphisms that are selected so as to be outside of tandem repeat regions, and are not genetically linked.
摘要翻译：本发明提供了用于分析受损核酸样品的方法和组合物。本发明还包括选择单核苷酸多态性的面板和面板的方法，所述单核苷酸多态性选择为不在串联重复区域之外，并且不是基因连接的。

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