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1. 发明申请

US20050060775A1 Production of human coagulation factor VIII from plant cells and whole plants 审中-公开
标题翻译：从植物细胞和整株植物生产人凝血因子VIII
公开(公告)号：US20050060775A1
公开(公告)日：2005-03-17
申请号：US10863969
申请日：2004-06-08
申请人： Brian Hooker , Daniel Anderson , Johnway Gao , Ziyu Dai
发明人： Brian Hooker , Daniel Anderson , Johnway Gao , Ziyu Dai
IPC分类号： A01H1/00 , C12N15/82
CPC分类号： C12N15/8257
摘要： The invention includes methods for production of a polypeptide having factor VIII activity by introduction of a polynucleotide construct into a plant cell. The construct includes an encoding sequence for a polypeptide of coagulation factor VIII or a functional variant thereof. The plant cell is cultured or regenerated into a plant and the polypeptide or functional variant of factor VIII is expressed therein. The invention also includes vectors, plant cells, plant tissues, plants and seeds containing a polynucleotide sequence encoding a functional variant of human coagulation factor VIII. The invention further includes a recombinant DNA molecule having a promoter which is functional in plants operably linked to a coding sequence which codes for a polynucleotide having coagulation factor VIII activity.
摘要翻译：本发明包括通过将多核苷酸构建体引入植物细胞来生产具有因子VIII活性的多肽的方法。该构建体包含凝血因子VIII多肽或其功能变体的编码序列。将植物细胞培养或再生成植物，并在其中表达因子VIII的多肽或功能变体。本发明还包括含有编码人凝血因子VIII功能变体的多核苷酸序列的载体，植物细胞，植物组织，植物和种子。本发明还包括具有启动子的重组DNA分子，该启动子在与可编码具有凝血因子VIII活性的多核苷酸的编码序列可操作地连接的植物中是功能性的。

2. 发明授权

US06783978B1 Isolation and characterization of the genomic DNA clones of ribosomal protein gene L25 in tobacco 失效
标题翻译：烟草中核糖体蛋白基因L25的基因组DNA克隆的分离和表征
公开(公告)号：US06783978B1
公开(公告)日：2004-08-31
申请号：US09714948
申请日：2000-11-20
申请人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
发明人： Lifang Shi , Johnway Gao , Brian S. Hooker , Ziyu Dai
IPC分类号： C12N1500
CPC分类号： C12N15/8234 , C12N15/8225
摘要： The promoter of the ribosomal protein gene L25 is operably linked to a structural gene. This chimeric gene is placed in an expression vector. The expression vector containing the chimeric gene is used to transform plants cells and plants. Seeds are obtained from the transformed plants. A product, such as a protein, encoded by the structural gene is isolated from the transformed plant cells and plants.
摘要翻译：核糖体蛋白基因L25的启动子与结构基因可操作地连接。将该嵌合基因置于表达载体中。含有嵌合基因的表达载体用于转化植物细胞和植物。从转化的植物获得种子。从转化的植物细胞和植物中分离由结构基因编码的产物，如蛋白质。

3. 发明授权

US06355864B1 Versatile rpL34 promoter elements and use thereof 失效
标题翻译：多功能rpL34启动子元件及其用途
公开(公告)号：US06355864B1
公开(公告)日：2002-03-12
申请号：US09417019
申请日：1999-10-12
申请人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
发明人： Lifang Shi , Ziyu Dai , Johnway Gao , Brian S. Hooker , Daniel B. Anderson
IPC分类号： C07H2104
CPC分类号： C12N15/8238 , C12N15/8216 , C12N15/8229 , C12N15/8237 , C12N15/8239
摘要： Synergistically functional, yet separable, cis-acting enhancer elements from the rpL34 promoter are disclosed. These enhancer elements of the instant invention may be used in combination with a plurality of promoters to increase gene expression without affecting the intrinsic specificity of the promoters. Also disclosed are methods for using the enhancer elements of the instant invention as well as vectors and transgenic plants comprising the enhancer elements.
摘要翻译：公开了来自rpL34启动子的协同作用的，但可分离的顺式作用的增强子元件。本发明的这些增强子元件可以与多个启动子组合使用以增加基因表达而不影响启动子的固有特异性。还公开了使用本发明的增强子元件以及包含增强子元件的载体和转基因植物的方法。

4. 发明申请

US20050005324A1 Gene promoters isolated from potato and use thereof 审中-公开
标题翻译：从马铃薯分离的基因启动子及其用途
公开(公告)号：US20050005324A1
公开(公告)日：2005-01-06
申请号：US10903948
申请日：2004-07-30
申请人： Ziyu Dai , LiFang Shi , Brian Hooker
发明人： Ziyu Dai , LiFang Shi , Brian Hooker
IPC分类号： C12N9/10 , C12N15/29 , C12N15/82 , C23C16/44 , C23C16/455 , C07H21/04 , C12N5/10 , C12N15/09 , C12N15/63 , C12N15/87
CPC分类号： C23C16/45565 , C12N9/1096 , C12N15/8237 , C12N15/8238 , C23C16/45519
摘要： There are disclosed novel pin1 gene promoter isoforms and amt gene promoter isoforms. The promoters and their functional elements may be used independently or in combination with one or more enhancer elements to increase or otherwise manipulate gene expression. The promoters disclosed may be used in Controlled Environment Agriculture for heterologous protein production. Also disclosed are methods for using the promoter and promoter elements of the instant invention, as well as vectors and transgenic plants comprising the same.
摘要翻译：已经公开了新的pin1基因启动子同种型和amt基因启动子同种型。启动子及其功能元件可以单独使用或与一种或多种增强子元件组合使用以增加或以其他方式操作基因表达。所公开的启动子可用于受控环境农业用于异源蛋白质生产。还公开了使用本发明的启动子和启动子元件以及包含其的载体和转基因植物的方法。

5. 发明授权

US07449569B2 Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 失效
标题翻译：分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法
公开(公告)号：US07449569B2
公开(公告)日：2008-11-11
申请号：US10920625
申请日：2004-08-17
申请人： Ziyu Dai , Linda L. Lasure , Jon K. Magnuson
发明人： Ziyu Dai , Linda L. Lasure , Jon K. Magnuson
IPC分类号： C07H21/04 , C12Q1/68 , C12N15/00 , C12N15/63 , C12N1/00
CPC分类号： C12P7/44 , C07K14/38 , C12N15/80 , C12P7/46 , C12P7/48 , C12P7/56 , C12P7/58 , C12P17/06 , C12P21/005 , C12P35/00 , C12P37/00
摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
摘要翻译：本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。本发明还包括利用真菌进行蛋白质或化学生产的方法。通过用重组多核苷酸分子转化真菌产生转化的真菌。重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

6. 发明申请

US20060040342A1 Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 失效
标题翻译：分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法
公开(公告)号：US20060040342A1
公开(公告)日：2006-02-23
申请号：US10920625
申请日：2004-08-17
申请人： Ziyu Dai , Linda Lasure , Jon Magnuson
发明人： Ziyu Dai , Linda Lasure , Jon Magnuson
IPC分类号： C12P37/00 , C12P21/06 , C12P17/06 , C12P7/62 , C12P7/46 , C12N1/18 , C12N15/74
CPC分类号： C12P7/44 , C07K14/38 , C12N15/80 , C12P7/46 , C12P7/48 , C12P7/56 , C12P7/58 , C12P17/06 , C12P21/005 , C12P35/00 , C12P37/00
摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
摘要翻译：本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。本发明还包括利用真菌进行蛋白质或化学生产的方法。通过用重组多核苷酸分子转化真菌产生转化的真菌。重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

7. 发明申请

US20090068723A1 Isolated Fungal Promoters and Gene Transcription Terminators and Methods of Protein and Chemical Production in a Fungus 有权
标题翻译：分离的真菌启动子和基因转录终止子和真菌中蛋白质和化学生产的方法
公开(公告)号：US20090068723A1
公开(公告)日：2009-03-12
申请号：US12257261
申请日：2008-10-23
申请人： Ziyu Dai , Linda L. Lasure , Jon K. Magnuson
发明人： Ziyu Dai , Linda L. Lasure , Jon K. Magnuson
IPC分类号： C12N1/21 , C12N15/11 , C12N15/00
CPC分类号： C12P7/44 , C07K14/38 , C12N15/80 , C12P7/46 , C12P7/48 , C12P7/56 , C12P7/58 , C12P17/06 , C12P21/005 , C12P35/00 , C12P37/00
摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
摘要翻译：本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。本发明还包括利用真菌进行蛋白质或化学生产的方法。通过用重组多核苷酸分子转化真菌产生转化的真菌。重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

8. 发明授权

US08735562B2 Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus 有权
标题翻译：分离的真菌启动子和基因转录终止子以及真菌中蛋白质和化学生产的方法
公开(公告)号：US08735562B2
公开(公告)日：2014-05-27
申请号：US12257261
申请日：2008-10-23
申请人： Ziyu Dai , Linda L. Lasure , Jon K. Magnuson
发明人： Ziyu Dai , Linda L. Lasure , Jon K. Magnuson
IPC分类号： C07H21/04 , C12N15/63
CPC分类号： C12P7/44 , C07K14/38 , C12N15/80 , C12P7/46 , C12P7/48 , C12P7/56 , C12P7/58 , C12P17/06 , C12P21/005 , C12P35/00 , C12P37/00
摘要： The present invention encompasses isolated gene regulatory elements and gene transcription terminators that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention also encompasses a method of utilizing a fungus for protein or chemical production. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to another molecule comprising a coding region of a gene of interest. The gene regulatory element and gene transcription terminator may temporally and spatially regulate expression of particular genes for optimum production of compounds of interest in a transgenic fungus.
摘要翻译：本发明包括在天然真菌中差异表达的分离的基因调控元件和基因转录终止子，其表现出相对于表现出第二形态的天然真菌的第一形态。本发明还包括利用真菌进行蛋白质或化学生产的方法。通过用重组多核苷酸分子转化真菌产生转化的真菌。重组多核苷酸分子含有与包含感兴趣的基因的编码区的另一个分子可操作地连接的分离的多核苷酸序列。基因调控元件和基因转录终止子可以在时间上和空间上调节特定基因的表达，以在转基因真菌中最佳产生感兴趣的化合物。

9. 发明申请

US20090042302A1 Isolated Polynucleotides and Methods of Promoting a Morphology in a Fungus 审中-公开
标题翻译：分离的多核苷酸和促进真菌形态学的方法
公开(公告)号：US20090042302A1
公开(公告)日：2009-02-12
申请号：US12237292
申请日：2008-09-24
申请人： Linda L. Lasure , Ziyu Dai
发明人： Linda L. Lasure , Ziyu Dai
IPC分类号： C12N15/74
CPC分类号： C07H21/04 , C07K14/38 , C12N1/14
摘要： The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
摘要翻译：本发明包括在表现出具有第二形态的天然真菌的第一形态的天然真菌中差异表达的分离的多核苷酸分子。本发明包括利用真菌增强生物过程的方法。通过用重组多核苷酸分子转化真菌产生转化的真菌。重组多核苷酸分子含有与启动子可操作地连接的分离的多核苷酸序列。表达多核苷酸序列以促进第一形态。转化真菌的第一种形态使用第二种形态增强了相对于生物过程的生物过程。

10. 发明授权

US07439067B2 Isolated polynucleotides and methods of promoting a morphology in a fungus 失效
标题翻译：分离的多核苷酸和促进真菌形态的方法
公开(公告)号：US07439067B2
公开(公告)日：2008-10-21
申请号：US10442017
申请日：2003-05-19
申请人： Linda L. Lasure , Ziyu Dai
发明人： Linda L. Lasure , Ziyu Dai
IPC分类号： C12N15/80 , C12P7/48
CPC分类号： C07H21/04 , C07K14/38 , C12N1/14
摘要： The invention includes isolated polynucleotide molecules that are differentially expressed in a native fungus exhibiting a first morphology relative to the native fungus exhibiting a second morphology. The invention includes a method of enhancing a bioprocess utilizing a fungus. A transformed fungus is produced by transforming a fungus with a recombinant polynucleotide molecule. The recombinant polynucleotide molecule contains an isolated polynucleotide sequence linked operably to a promoter. The polynucleotide sequence is expressed to promote a first morphology. The first morphology of the transformed fungus enhances a bioprocess relative to the bioprocess utilizing a second morphology.
摘要翻译：本发明包括在表现出具有第二形态的天然真菌的第一形态的天然真菌中差异表达的分离的多核苷酸分子。本发明包括利用真菌增强生物过程的方法。通过用重组多核苷酸分子转化真菌产生转化的真菌。重组多核苷酸分子含有与启动子可操作地连接的分离的多核苷酸序列。表达多核苷酸序列以促进第一形态。转化真菌的第一种形态使用第二种形态增强了相对于生物过程的生物过程。

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