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1. 发明授权

US4880749A Analytical element and its use in a whole blood hemoglobin assay 失效
标题翻译：分析元素及其在全血血红蛋白测定中的应用
公开(公告)号：US4880749A
公开(公告)日：1989-11-14
申请号：US617453
申请日：1984-06-05
申请人： Brent A. Burdick , Tai-Wing Wu , David A. Hilborn , Richard W. Spayd
发明人： Brent A. Burdick , Tai-Wing Wu , David A. Hilborn , Richard W. Spayd
IPC分类号： G01N21/78 , G01N31/22 , G01N33/52 , G01N33/72
CPC分类号： G01N33/525 , Y10S435/805
摘要： Disclosed herein is an assay useful for the quantitative determination of hemoglobin in whole blood. This assay utilizes a multizone element comprising a registration zone comprising particulate barium sulfate distributed in a "blush" polymer, and a spreading zone having a void volume and an average pore size (e.g. greater than 5 microns) effective to accommodate whole blood. The element also contains an interactive composition capable of converting substantially all forms of hemoglobin into a detectable end product. One particularly useful interactive composition comprises a hemoglobin oxidizing agent (e.g. ferricyanide) and thiocyanate. Another useful interactive composition comprises an ionic surfactant, e.g. sodium octyl sulfate or sodium dodecyl sulfate.
摘要翻译：本文公开了用于定量测定全血中血红蛋白的测定法。该测定法利用包含分布在“腮红”聚合物中的颗粒状硫酸钡的配准区的多区域元件，以及有效地适应全血的空隙体积和平均孔径（例如大于5微米）的铺展区。该元件还包含能够将基本上所有形式的血红蛋白转化成可检测的最终产物的交互式组合物。一种特别有用的相互作用组合物包含血红蛋白氧化剂（例如铁氰化物）和硫氰酸盐。另一种有用的交互组合物包括离子表面活性剂，例如辛基硫酸钠或十二烷基硫酸钠。

2. 发明授权

US5616299A Dispenser for dried biological reagent spheres 失效
标题翻译：干生物试剂球的分配器
公开(公告)号：US5616299A
公开(公告)日：1997-04-01
申请号：US469050
申请日：1995-06-06
申请人： David W. Walker , Brent A. Burdick , James F. Jolly , Daniel D. Zender
发明人： David W. Walker , Brent A. Burdick , James F. Jolly , Daniel D. Zender
IPC分类号： B01L99/00 , G01N35/00 , B01L11/00 , B65G29/00
CPC分类号： B01L99/00 , B01J2219/00468 , G01N2035/00574
摘要： An apparatus for dispensing reagent spheres from a vial includes a housing with an inlet through which the reagent spheres are received from the vial. Within the housing is a dispensing tube which forms a passageway for the reagent spheres and which has a first relatively large section in communication with the inlet and a funnel section in which the passageway reduces in cross section to a narrower linearizing section. The cross sectional area of the linearizing section is only slightly larger than one of the reagent spheres being dispensed. A metering shaft extends across and blocks the linearizing section of the passageway. The metering shaft has a depression for conveying one reagent sphere at a time between portions of linearizing section when the metering shaft is rotated 180 degrees. A gear on the metering shaft meshes with teeth on a plunger which is slidably mounted to the housing. A user pressing the plunger causes the metering shaft to rotate transferring a reagent sphere to a portion of the passageway connected to the outlet of the housing and thereby ejects that reagent sphere from the housing.
摘要翻译：用于从小瓶分配试剂球的装置包括具有入口的壳体，试剂球从小瓶接收。在壳体内部是分配管，其形成用于试剂球的通道，并且其具有与入口连通的第一相对大的部分和漏斗部分，其中通道在横截面上减小到较窄的线性化部分。线性化部分的横截面面积仅略微大于被分配的一种试剂球体。计量轴延伸穿过并阻塞通道的线性化部分。计量轴具有用于当计量轴旋转180度时一次在线性化部分的部分之间输送一个试剂球的凹陷。计量轴上的齿轮与可滑动地安装在壳体上的柱塞上的齿啮合。按压柱塞的使用者使得计量轴旋转，将试剂球转移到连接到壳体出口的通道的一部分，从而将该试剂球从壳体喷出。

3. 发明授权

US5702715A Reinforced biological sealants 失效
标题翻译：强化生物密封胶
公开(公告)号：US5702715A
公开(公告)日：1997-12-30
申请号：US549365
申请日：1995-10-27
申请人： Victor V. Nikolaychik , Brent A. Burdick , Leonid V. Nikolaychik
发明人： Victor V. Nikolaychik , Brent A. Burdick , Leonid V. Nikolaychik
IPC分类号： A61L24/00 , A61L24/10 , A01N25/34
CPC分类号： A61L24/106 , A61L24/0073
摘要： A reinforced biological sealant is disclosed. The sealant includes a fibrinogen composition adapted to be applied to a wound, and a solid thrombin composition layered on the fibrinogen film. At least one of the fibrinogen or thrombin compositions comprises a reinforcement filler. Means are provided for activating formation of a fibrin mesh from the fibrinogen and thrombin compositions, thereby effecting hermetization and hemostasis of the wound.
摘要翻译：公开了一种增强的生物密封剂。密封剂包括适于施用于伤口的纤维蛋白原组合物和层叠在纤维蛋白原膜上的固体凝血酶组合物。至少一种纤维蛋白原或凝血酶组合物包含增强填料。提供了用于激活纤维蛋白原和凝血酶组合物形成纤维蛋白网的手段，从而实现伤口的呼气和止血。

4. 发明授权

US5334499A Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction 失效
$Methods of extracting, amplifying and detecting a nucleic acid from whole blood or PBMC fraction$
标题翻译：从全血或PBMC级分提取，扩增和检测核酸的方法
公开(公告)号：US5334499A
公开(公告)日：1994-08-02
申请号：US406222
申请日：1989-09-12
申请人： Brent A. Burdick , Tobias D. Ekeze
发明人： Brent A. Burdick , Tobias D. Ekeze
IPC分类号： C07H1/06 , C07H21/00 , C07K14/16 , C07K14/74 , C07K14/805 , C12N15/10 , C12Q1/68 , G01N33/50 , G01N33/58 , G01N1/10
CPC分类号： C12N15/1003 , C07K14/005 , C07K14/70539 , C07K14/805 , C12Q1/6806 , C12N2740/16022 , C12Q1/703 , Y10S435/803 , Y10T436/25375
摘要： A nucleic acid is rapidly extracted from whole blood or a peripheral blood mononuclear cell (PBMC) fraction thereof. Extraction from the PBMC fraction is accomplished by heating the fraction at or near the boiling point of water for a few minutes and recovering the extracted nucleic acid. This rapid method is particularly useful for extracting DNA for the detection of genetic diseases or infectious agents, such as HIV-I. Whole blood can likewise be heated after it is mixed with a salt solution containing a polysaccharide, such as dextran. The extracted nucleic acid is then recovered from the heated mixture. Nucleic acids extracted in this way are available for amplification using a polymerase chain reaction. Where the presence of a specific gene is to be determined for diagnostic purposes, it can be extracted as described above and subjected to suitable amplification and detection steps.
摘要翻译：从全血或其外周血单核细胞（PBMC）部分快速提取核酸。从PBMC级分提取通过在水沸点附近加热馏分几分钟并回收提取的核酸来完成。这种快速方法对于提取用于检测遗传性疾病或感染因子如HIV-1的DNA特别有用。在与含有多糖（例如葡聚糖）的盐溶液混合后，同样可以加热全血。然后从加热的混合物中回收提取的核酸。以这种方式提取的核酸可用于使用聚合酶链反应进行扩增。如果要确定特定基因的存在用于诊断目的，则可以如上所述进行提取并进行适当的扩增和检测步骤。

5. 发明授权

US5599660A Method and preparation for sequential delivery of wax-embedded, inactivated biological and chemical reagents 失效
标题翻译：蜡嵌入的，灭活的生物和化学试剂的顺序递送的方法和准备
公开(公告)号：US5599660A
公开(公告)日：1997-02-04
申请号：US349115
申请日：1994-12-02
申请人： Rama P. Ramanujam , Brent A. Burdick , Ulf D. Landegren , Pierre Sevigny
发明人： Rama P. Ramanujam , Brent A. Burdick , Ulf D. Landegren , Pierre Sevigny
IPC分类号： C12N11/04 , C12Q1/68 , G01N33/543 , C10G73/36 , C12P19/34
CPC分类号： C12Q1/6848 , C12N11/04 , C12Q1/6806 , C12Q1/686 , G01N33/5436 , B01J2219/00504 , Y10T436/10 , Y10T436/143333
摘要： A method and preparation for the storage and delivery of purified reagents is disclosed. In one aspect, the preparation comprises an amount of a first wax carrier, the first wax carrier having a first melting point, and an amount of a first reagent, wherein first reagent is a substantially purified preparation of at least one biological or chemical reagent. The first wax carrier and the first reagent are combined to form a solid first reagent portion. The solid mixture is combined with a second reagent portion comprised of at least one biological or chemical reagent in an inactive form.
摘要翻译：公开了用于储存和递送纯化试剂的方法和制备方法。一方面，该制剂包含一定量的第一蜡载体，第一蜡载体具有第一熔点和第一试剂的量，其中第一试剂是至少一种生物或化学试剂的基本上纯化的制剂。将第一蜡载体和第一试剂合并形成固体第一试剂部分。将固体混合物与由至少一种非活性形式的生物或化学试剂组成的第二试剂部分组合。

6. 发明授权

US5132215A Method of making double-stranded DNA sequences 失效
标题翻译：制作双链DNA序列的方法
公开(公告)号：US5132215A
公开(公告)日：1992-07-21
申请号：US244871
申请日：1988-09-15
申请人： Krishna Jayaraman , Brent A. Burdick , Fred T. Oakes
发明人： Krishna Jayaraman , Brent A. Burdick , Fred T. Oakes
IPC分类号： C12P21/00 , C07K14/80 , C12N15/10 , C12Q1/68
CPC分类号： C12N15/10 , C07K14/80 , C12Q1/6806 , C12Q1/686
摘要： A method of synthesizing double-stranded DNA sequences is disclosed. The method comprises the steps of:(a) preparing a first series of oligodeoxyribonucleotide fragments which, when joined in proper sequence, form a DNA coding strand;(b) preparing a second series of oligodeoxyribonucleotide fragments which, when joined in proper sequence, form a DNA strand complementary to the coding strand;(c) compelling hydrogen bonding and ligation in proper sequence between the first and second series of oligodeoxyribonucleotide fragments prepared in steps (a) and (b) in a single reaction to produce the entire double-stranded DNA sequence;(d) treating the double-stranded DNA sequence with one oligonucleotide primer for each strand under hybridizing conditions;(e) polymerizing an extension product of each primer that is complementary to each strand of the double-stranded DNA sequence which is a template for forming the primer extension product;(f) denaturing the product of step (e) to separate the primer extension products from their respective templates to form four separate single-stranded DNA sequences;(g) treating the denatured product of (f) with oligonucleotide primers, such that a primer extension product is synthesizing using each of the single strands produced in step (f) as a template resulting in amplification of the double-stranded DNA sequence; and(h) repeating steps (d), (e), (f) and (g) until the desired quantity of the double-stranded DNA sequence is formed.

7. 发明授权

US5045477A Analytical methods utilizing reducible components 失效
标题翻译：利用可还原成分的分析方法
公开(公告)号：US5045477A
公开(公告)日：1991-09-03
申请号：US344870
申请日：1989-04-28
申请人： Robert T. Belly , Albert J. Mura , Theodore W. Esders , Brent A. Burdick
发明人： Robert T. Belly , Albert J. Mura , Theodore W. Esders , Brent A. Burdick
IPC分类号： C12Q1/00 , C12Q1/04 , G01N31/22 , G01N33/52
CPC分类号： G01N31/221 , C12Q1/00 , C12Q1/04 , G01N31/22 , G01N33/52 , C12Q2304/26
摘要： Certain reducible compounds are useful in analytical compositions, elements and methods, e.g. for assays of bacterial cells. These compounds comprise a moiety which provides a detectable species (e.g. a dye) when released from the compound in an environment of pH 9 or less (i.e. physiological pH). Structurally, the reducible compounds are aromatic derivatives or quinones having suitable substituents which promote varying amounts of moiety release at physiological pH. When reduced at about pH 7, the preferred compounds release at least 50% of the available detectable species within 30 minutes.
摘要翻译：某些可还原的化合物可用于分析组合物，元素和方法，例如，用于细菌细胞的测定。这些化合物包括当在pH 9或更低（即生理pH）的环境中从化合物中释放时提供可检测物质（例如染料）的部分。在结构上，可还原的化合物是具有合适取代基的芳香族衍生物或醌，其在生理pH下促进不同量的部分释放。当在约pH7下降低时，优选的化合物在30分钟内释放至少50％的可检测物质。

8. 发明授权

US4801504A Fluorescent labels having a polysaccharide bound to polymeric particles 失效
标题翻译：具有多糖结合聚合物颗粒的荧光标记
公开(公告)号：US4801504A
公开(公告)日：1989-01-31
申请号：US100513
申请日：1987-09-24
申请人： Brent A. Burdick , Susan J. Danielson
发明人： Brent A. Burdick , Susan J. Danielson
IPC分类号： C08F8/00 , C08G81/00 , C08G81/02 , G01N33/52 , G01N33/533 , G01N33/543 , G01N33/545 , G01N33/58 , B32B5/16
CPC分类号： G01N33/582 , G01N33/525 , G01N33/533 , G01N33/54386 , G01N33/585 , Y10S436/80 , Y10S436/805 , Y10S436/808 , Y10T428/2991
摘要： Fluorescent labels comprise a polysaccharide bound to a polymeric particle which contains a fluorescent rare earth chelate. These labels can be attached to any of a variety of physiologically reactive species to provide labeled species which have improved stability in aqueous solutions. The labeled species are particularly useful in specific binding assays to determine an immunologically reactive ligand, e.g. a hapten, in either solution or dry analytical techniques.
摘要翻译：荧光标记物包含与含有荧光稀土螯合物的聚合物颗粒结合的多糖。这些标签可以连接到各种生理反应性物质中的任一种，以提供在水溶液中具有改善的稳定性的标记物质。标记的物种在特异性结合测定中特别有用，以确定免疫反应性配体，例如，半抗原，溶液或干法分析技术。

9. 发明授权

US4788140A Analytical element containing photosensitive compound and filter layer and method of use 失效
标题翻译：含有光敏化合物和过滤层的分析元素及使用方法
公开(公告)号：US4788140A
公开(公告)日：1988-11-29
申请号：US830036
申请日：1986-02-18
申请人： John B. Findlay , Brent A. Burdick , Ronald A. Wellman , Mark E. Shafer
发明人： John B. Findlay , Brent A. Burdick , Ronald A. Wellman , Mark E. Shafer
IPC分类号： C12Q1/00 , C12Q1/44 , C12Q1/50 , G01N33/52 , C12Q1/28
CPC分类号： G01N33/525 , C12Q1/50 , Y10S435/805
摘要： A multilayer analytical element for the determination of a clinically significant enzyme analyte comprises a photosensitive compound (e.g. a photosensitive dye or dye precursor) and a filter layer containing one or more filter dyes. The filter layer is situated in the element such that incident radiation used to detect a density change resulting from interaction of the analyte and the photosensitive compound passes through the filter layer before it reaches the photosensitive compound. The use of the filter layer inhibits premature changes in the photosensitive compound caused by incident radiation. This element is particularly useful for the determination of creatine kinase or one of its isoenzymes, e.g. creatine kinase-MB.

10. 发明授权

US5374516A Avidin-and biotin immobilized reagents and methods of use 失效
标题翻译：抗生物素蛋白和生物素固定化试剂及使用方法
公开(公告)号：US5374516A
公开(公告)日：1994-12-20
申请号：US315086
申请日：1989-02-24
申请人： Richard C. Sutton , Brent A. Burdick , Susan J. Danielson , Harold C. Warren, III , Brian A. Snyder , Gregory J. McClune , Annie L. Wu
发明人： Richard C. Sutton , Brent A. Burdick , Susan J. Danielson , Harold C. Warren, III , Brian A. Snyder , Gregory J. McClune , Annie L. Wu
IPC分类号： G01N33/543 , G01N33/545 , G01N33/546
CPC分类号： G01N33/54306 , G01N33/54313 , G01N33/545 , Y10T428/2991 , Y10T428/2998
摘要： Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.
摘要翻译：试剂由水不溶性聚合物颗粒制备，共价连接的抗生物素蛋白，生物素或抗生物素蛋白或生物素衍生物。聚合物颗粒在至少外表面上包含衍生自至少一种具有反应性活化的2-取代的乙基磺酰基，乙烯基磺酰基或活性卤素原子的烯属不饱和单体的聚合物。亲和素，生物素或抗生物素蛋白或生物素衍生物的共价连接通过这些反应性基团直接或间接地实现。所得试剂可用于分析元件和各种分析方法，包括凝集和夹心测定。固定的抗生物素蛋白，生物素或衍生物可用于与可能与生物学兴趣的化合物缀合的相应生物素或抗生物素蛋白分子复合。

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