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1. 发明授权

US5374516A Avidin-and biotin immobilized reagents and methods of use 失效
标题翻译：抗生物素蛋白和生物素固定化试剂及使用方法
公开(公告)号：US5374516A
公开(公告)日：1994-12-20
申请号：US315086
申请日：1989-02-24
申请人： Richard C. Sutton , Brent A. Burdick , Susan J. Danielson , Harold C. Warren, III , Brian A. Snyder , Gregory J. McClune , Annie L. Wu
发明人： Richard C. Sutton , Brent A. Burdick , Susan J. Danielson , Harold C. Warren, III , Brian A. Snyder , Gregory J. McClune , Annie L. Wu
IPC分类号： G01N33/543 , G01N33/545 , G01N33/546
CPC分类号： G01N33/54306 , G01N33/54313 , G01N33/545 , Y10T428/2991 , Y10T428/2998
摘要： Reagents have been prepared from water-insoluble polymeric particles to which are covalently attached avidin, biotin or an avidin or biotin derivative. The polymeric particles comprise a polymer on at least the outer surface which is derived from at least one ethylenically unsaturated monomer having a reactive activated 2-substituted ethylsulfonyl, vinylsulfonyl or active halogen atom. Covalent attachment of avidin, biotin or an avidin or biotin derivative is effected either directly or indirectly through these reactive groups. The resulting reagent is useful in analytical elements and various analytical methods including agglutination and sandwich assays. The immobilized avidin, biotin or derivative can be used to complex with the corresponding biotin or avidin molecule which may be conjugated to a compound of biological interest.
摘要翻译：试剂由水不溶性聚合物颗粒制备，共价连接的抗生物素蛋白，生物素或抗生物素蛋白或生物素衍生物。聚合物颗粒在至少外表面上包含衍生自至少一种具有反应性活化的2-取代的乙基磺酰基，乙烯基磺酰基或活性卤素原子的烯属不饱和单体的聚合物。亲和素，生物素或抗生物素蛋白或生物素衍生物的共价连接通过这些反应性基团直接或间接地实现。所得试剂可用于分析元件和各种分析方法，包括凝集和夹心测定。固定的抗生物素蛋白，生物素或衍生物可用于与可能与生物学兴趣的化合物缀合的相应生物素或抗生物素蛋白分子复合。

2. 发明授权

US4885240A Use of organic buffers to reduce dehydroascorbic acid interference in analytical methods 失效
标题翻译：在分析方法中使用有机缓冲液来减少脱氢抗坏血酸的干扰
公开(公告)号：US4885240A
公开(公告)日：1989-12-05
申请号：US903532
申请日：1986-09-04
申请人： Annie L. Wu
发明人： Annie L. Wu
IPC分类号： G01N33/52 , C12Q1/00 , C12Q1/04 , C12Q1/28
CPC分类号： C12Q1/28 , C12Q1/04 , C12Q2304/26 , Y10S435/805 , Y10S435/81 , Y10S436/805 , Y10S436/904 , Y10T436/142222
摘要： The interference of dehydroascorbic acid, an oxidation product of ascorbic acid, in analytical methods is reduced with the use of certain nitrogen-containing organic buffers. Dehydroascorbic acid tends to cause premature reduction of reducible compounds which are used to provide detectable species in the presence of an analyte. These organic buffers, as opposed to inorganic buffers, prevent premature reduction of the reducible compounds. An analytical method can be carried out in solution or in a dry analytical element.
摘要翻译：脱氢抗坏血酸（抗坏血酸的氧化产物）在分析方法中的干扰通过使用某些含氮有机缓冲液而降低。脱氢抗坏血酸倾向于引起可还原化合物的过早还原，其用于在分析物存在下提供可检测的物质。与无机缓冲液相反，这些有机缓冲剂防止可还原化合物的过早还原。分析方法可以在溶液或干燥分析元件中进行。

3. 发明授权

US5358852A Use of calcium in immunoassay for measurement of C-reactive protein 失效
标题翻译：在免疫测定中使用钙来测定C-反应蛋白
公开(公告)号：US5358852A
公开(公告)日：1994-10-25
申请号：US993569
申请日：1992-12-21
申请人： Annie L. Wu
发明人： Annie L. Wu
IPC分类号： G01N33/543 , G01N33/68 , G01N33/531 , G01N30/91
CPC分类号： G01N33/6863 , G01N33/54393 , G01N2333/4737
摘要： A specific binding immunoassay method for reducing the "hook" effect for the measurement of C-Reactive Protein has been discovered for both solution and dry analytical elements comprising contacting a liquid sample containing C-reactive protein in the presence of calcium ions with (a) a first antibody Ab1 specific for C-reactive protein, Ab1 being immobilized on a water-insoluble substrate and (b) a labeled, unbound second antibody Ab2 specific for C-reactive protein to obtain a water-insoluble complex of Ab1, ligand, and Ab2; (2) separating the water-insoluble complex from the liquid sample and unreacted Ab2; and (3) measuring either the amount of Ab2 associated with said water-insoluble complex or the amount of unreacted Ab2 as an indication of the amount of C-reactive protein in the sample.
摘要翻译：已经发现用于降低C反应蛋白测量的“钩”效应的特异性结合免疫测定方法用于溶液和干分析元件，包括在钙离子存在下使含有C反应蛋白的液体样品与（a）对C反应蛋白特异的第一抗体Ab1，Ab1固定在水不溶性底物上，（b）对C反应蛋白特异性的标记的未结合的第二抗体Ab2，以获得Ab1，配体和 Ab2; （2）从液体样品和未反应的Ab2分离水不溶性络合物; 和（3）测量与所述水不溶性络合物相关的Ab2的量或未反应的Ab2的量作为样品中C-反应蛋白的量的指示。

4. 发明授权

US4713327A Determination of total creatine kinase or an isoenzyme with a multilayer analytical element 失效
标题翻译：用多层分析元素测定总肌酸激酶或同工酶
公开(公告)号：US4713327A
公开(公告)日：1987-12-15
申请号：US729331
申请日：1985-05-01
申请人： John B. Findlay , Annie L. Wu , Gary E. Norton
发明人： John B. Findlay , Annie L. Wu , Gary E. Norton
IPC分类号： G01N33/70 , C12Q1/50 , G01N33/52 , G01N33/573 , C12M1/38 , G01N33/50
CPC分类号： C12Q1/50 , G01N33/526 , G01N33/573
摘要： A multilayer analytical element for the determination of creatine kinase comprises a support having thereon, in order and in fluid contact, a registration layer and an isotropically porous spreading layer. The registration layer contains a binder material at a coverage of at least about 8 g/m.sup.2, which coverage improves the stability and keeping properties of the element in high humidity environments. This element is useful in an analytical method for determining total creatine kinase or any one of its isoenzymes, e.g. creatine kinase-MB.
摘要翻译：用于测定肌酸激酶的多层分析元件包括其上依次并且以流体接触的载体，其配准层和各向同性多孔扩散层。配准层包含至少约8g / m 2的覆盖层的粘合剂材料，该覆盖物提高元件在高湿度环境中的稳定性和保持性能。该元件可用于确定总肌酸激酶或其任何一种同工酶的分析方法，例如，肌酸激酶-MB。

5. 发明授权

US4703002A Method for preparing coating compositions containing an immunologically reactive species and elements containing same 失效
标题翻译：制备含有免疫反应性物质和含有其的元件的涂料组合物的方法
公开(公告)号：US4703002A
公开(公告)日：1987-10-27
申请号：US729332
申请日：1985-05-01
申请人： John B. Findlay , Annie L. Wu
发明人： John B. Findlay , Annie L. Wu
IPC分类号： G01N33/543 , A61K39/00 , C07K17/02 , G01N33/545 , G01N33/573 , G01N33/548 , G01N33/549
CPC分类号： G01N33/545 , G01N33/573 , Y10S435/808 , Y10S436/81
摘要： A method for preparing a blush polymer coating composition containing an immunologically reative species comprises milling the species and the other materials used in the composition to uniformly disperse the species therein. This coating composition can be used to prepare analytical elements for determining analytes (e.g. creating kinase-MB) whereby the effects of potential interferents are immunochemically removed. By milling the immunologically reactive species, e.g. antisera, into the coating composition, the resulting element has improved stability resulting in improved keeping in high humidity environments.
摘要翻译：制备含有免疫学物种的腮红聚合物涂料组合物的方法包括研磨组合物中使用的物质和其它材料以均匀地分散其中的物质。该涂料组合物可用于制备用于测定分析物的分析元件（例如产生激酶-MB），由此潜在干扰物的作用被免疫化学去除。通过研磨免疫反应性物质，例如抗血清进入涂料组合物中，所得到的元件具有改善的稳定性，从而改善在高湿度环境中的保持性。

6. 发明授权

US5387510A Detection of amplified nucleic acid using secondary capture oligonucleotides and test kit 失效
标题翻译：使用二次捕获寡核苷酸和测试试剂盒检测扩增的核酸
公开(公告)号：US5387510A
公开(公告)日：1995-02-07
申请号：US769624
申请日：1991-10-02
申请人： Annie L. Wu
发明人： Annie L. Wu
IPC分类号： C12N15/09 , C12Q1/68 , G01N33/50 , G01N33/58 , C07H17/00 , C12P19/34
CPC分类号： C12Q1/6813 , C12Q1/6832 , Y10S435/81
摘要： A target nucleic acid can be amplified and detected using a water-insoluble capture probe having a short oligonucleotide (15-20 nucleotides) covalently attached to a polymeric particle, and one or more secondary oligonucleotides. The capture probe and secondary oligonucleotides are complementary to the target nucleic acid, although the sequences of complementarity are different. Moreover, each of the secondary oligonucleotides has a length which is equal to or greater than the length of the capture probe oligonucleotide. The secondary oligonucleotides are hybridized to the target nucleic acid either before or during hybridization of the target to the capture probe. The result is improved hybridization efficiency of capture probe and target nucleic acid.
摘要翻译：可以使用共价连接到聚合物颗粒的短寡核苷酸（15-20个核苷酸）和一种或多种二级寡核苷酸的水不溶性捕获探针扩增和检测靶核酸。捕获探针和次级寡核苷酸与靶核酸互补，尽管互补序列不同。此外，每个二级寡核苷酸的长度等于或大于捕获探针寡核苷酸的长度。在目标与捕获探针的杂交之前或期间，将二级寡核苷酸与靶核酸杂交。结果是提高了捕获探针和靶核酸的杂交效率。

7. 发明授权

US5387505A Preparation and isolation of single-stranded biotinylated nucleic acids by heat avidin-biotin cleavage 失效
标题翻译：通过热抗生物素蛋白 - 生物素切割制备和分离单链生物素化核酸
公开(公告)号：US5387505A
公开(公告)日：1995-02-07
申请号：US519533
申请日：1990-05-04
申请人： Annie L. Wu
发明人： Annie L. Wu
IPC分类号： C12N15/09 , C07K14/00 , C12Q1/68 , G01N33/53 , C12D1/08 , C07H5/04 , C07H17/00
CPC分类号： C12Q1/6806 , C12Q1/686
摘要： A biotinylated target nucleic acid can be isolated from a mixture of nucleic acids using a capture reagent comprising avidin. The targeted nucleic acid is complexed with avidin on a substrate and separated from uncomplexed materials. The complex is heated at above about 65.degree. C. for a suitable time to cleave the avidin-biotin bonds to release the desired targeted nucleic acid. Upon release, the targeted nucleic acid can be collected or detected in a suitable manner, such as with a complementary probe. This method is also useful for preparing single-stranded DNA for a number of uses.
摘要翻译：可以使用包含抗生物素蛋白的捕获试剂从核酸的混合物中分离生物素化的靶核酸。靶向核酸与底物上的抗生物素蛋白复合，并与未复合材料分离。将复合物在高于约65℃加热适宜的时间以切割抗生物素蛋白 - 生物素键以释放所需的靶向核酸。释放后，靶向核酸可以以合适的方式收集或检测，例如用互补探针。该方法也可用于制备用于多种用途的单链DNA。

8. 发明授权

US4952495A Hydrolyzable compounds which release electron transfer agents and analytical use of same 失效
标题翻译：释放电子转移剂的水解化合物及其分析用途
公开(公告)号：US4952495A
公开(公告)日：1990-08-28
申请号：US59667
申请日：1987-06-08
申请人： Robert T. Belly , Patricia M. Scensny , Annie L. Wu , Chung-yuan Chen
发明人： Robert T. Belly , Patricia M. Scensny , Annie L. Wu , Chung-yuan Chen
IPC分类号： C07D241/46 , C07H15/203 , C12Q1/34 , G01N33/58
CPC分类号： C07H15/203 , C07D241/46 , C12Q1/34 , G01N33/581 , C12Q2304/26 , C12Q2334/00 , G01N2333/924
摘要： Hydrolyzable substrates comprise blocked moieties which, when cleaved from the substrate during hydrolysis, provide electron transfer agents. The released electron transfer agents can be recycled between a reductant and a reducible compound that upon reduction provides a detectable species. Alternatively, they can be recycled between an oxidant and an oxidizable compound that upon oxidation provides a detectable species. These substrates are useful in analytical compositions, elements and methods for the determination of hydrolytic analytes, such as hydrolytic enzymes or biological cells containing such enzymes.

9. 发明授权

US4803160A Use of polymeric mordants to increase the intensity of rigid fluorescent dyes 失效
标题翻译：使用聚合物媒染剂来增加刚性荧光染料的强度
公开(公告)号：US4803160A
公开(公告)日：1989-02-07
申请号：US824755
申请日：1986-01-31
申请人： Annie L. Wu
发明人： Annie L. Wu
IPC分类号： C09B3/78 , C07C69/017 , C09B3/00 , C09B57/00 , C09B59/00 , C12Q1/04 , G01N21/76 , G01N33/04 , G01N33/50 , G01N33/52 , C12Q1/02
CPC分类号： C12Q1/04 , C09B3/00 , C09B57/00 , G01N33/5005 , G01N33/525 , C12Q2304/20 , C12Q2304/26 , G01N33/04 , Y10S435/968 , Y10S436/80 , Y10S436/805
摘要： Polymeric mordants are used with certain rigid fluorescent dyes or precursors thereof to increase fluorescence intensity. Compositions and analytical elements containing the mordants and dyes or dye precursors are useful in biological studies, such as cell and tissue staining and cell cytometry, and in analytical determinations for various analytes, e.g. living organisms.
摘要翻译：聚合媒染剂与某些刚性荧光染料或其前体一起使用以增加荧光强度。含有媒染剂和染料或染料前体的组合物和分析元件可用于生物学研究，例如细胞和组织染色和细胞计数，以及各种分析物的分析测定，例如，生物体。

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